scholarly journals Analysis of Unusual Sulfated Constituents and Anti-infective Properties of Two Indonesian Mangroves, Lumnitzera littorea and Lumnitzera racemosa (Combretaceae)

Separations ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 82
Author(s):  
Jeprianto Manurung ◽  
Jonas Kappen ◽  
Jan Schnitzler ◽  
Andrej Frolov ◽  
Ludger A. Wessjohann ◽  
...  

Lumnitzera littorea and Lumnitzera racemosa are mangrove species distributed widely along the Indonesian coasts. Besides their ecological importance, both are of interest owing to their wealth of natural products, some of which constitute potential sources for medicinal applications. We aimed to discover and characterize new anti-infective compounds, based on population-level sampling of both species from across the Indonesian Archipelago. Root metabolites were investigated by TLC, hyphenated LC-MS/MS and isolation, the internal transcribed spacer (ITS) region of rDNA was used for genetic characterization. Phytochemical characterization of both species revealed an unusual diversity in sulfated constituents with 3,3’,4’-tri-O-methyl-ellagic acid 4-sulfate representing the major compound in most samples. None of these compounds was previously reported for mangroves. Chemophenetic comparison of L. racemosa populations from different localities provided evolutionary information, as supported by molecular phylogenetic evidence. Samples of both species from particular locations exhibited anti-bacterial potential (Southern Nias Island and East Java against Gram-negative bacteria, Halmahera and Ternate Island against Gram-positive bacteria). In conclusion, Lumnitzera roots from natural mangrove stands represent a promising source for sulfated ellagic acid derivatives and further sulfur containing plant metabolites with potential human health benefits.

2018 ◽  
Vol 19 (4) ◽  
pp. 1303-1312
Author(s):  
RIGNOLDA DJAMALUDDIN

Djamaluddin R. 2018. The mangrove flora and their physical habitat characteristics in Bunaken National Park, NorthSulawesi, Indonesia. Biodiversitas 19: 1303-1312. The mangrove forests of Bunaken National Park are among the most distinctive andunusual in Southeast Asia because of the species that the forests contain. This study investigated the identity and diversity of mangroveplants as well as physiographic factors and major physical processes of every type of sub-habitats. Seven surveys were conducted tocollect and identify mangrove species of the park. Sub-habitats where specimen was found, aspects related to tidal inundation, nature ofsoil, freshwater influence and topography were observed as well as major physical processes influencing the condition of each subhabitat.The results suggested that the park was floristically rich with at least 27 plant species and they were distributed over tenrecognized sub-habitat types in different composition and diversity. Ceriops zippeliana Blume, Lumnitzera racemosa Willd, Lumnitzeralittorea (Jack) Voigt., Sonneratia ovata Backer, and Camptostemon philippinense (Vidal) Becc. were found in Bunaken National Parkand their presence confirmed the broader distribution limit of these species within Indo-Malesia region. A special notice was for C.philippinense as the distribution limit of this is rarely reported.


2010 ◽  
Vol 23 (4) ◽  
pp. 248 ◽  
Author(s):  
Jasmine K. Janes ◽  
Dorothy A. Steane ◽  
René E. Vaillancourt ◽  
Marco F. Duretto

In the past decade, two major classification schemes of the subtribe Pterostylidinae have resulted in taxonomic confusion because a single well known genus was split into a large number of new genera and subgenera, many of which are difficult to discriminate accurately. These classifications have not been accepted widely among systematists because of poor phylogenetic support for several genera. Analyses of the internal transcribed spacer (ITS) region of nuclear rDNA in a large number of species and samples facilitate further clarification of the relationships within the Pterostylidinae. The phylogenetic trees were reconstructed using parsimony and Bayesian methods. These phylogenetic trees indicate that subtribe Pterostylidinae is monophyletic, and support the concept of a single genus, Pterostylis R.Br. sensu lato within the Pterostylidinae. Two clades representing subgenera correlate with the morphology of the lateral sepals, whereas several of the previously erected genera consistently have poor support. The proposed subgenera are divided further into 10 sections. Several closely related taxa with identical ITS sequences require further scrutiny by population-level molecular techniques to determine their taxonomic status.


2016 ◽  
Vol 4 (6) ◽  
pp. 307-310
Author(s):  
Dhwani T. Dave ◽  
◽  
Gaurang B. Shah ◽  

Since ancient times, plants have been serving as a promising source of medicines and in recent times, extensive research has been made to isolate, characterize and screen a number of phytoconstituents/secondary plant metabolites for their pharmacological activities and safety in various disease models – both in vitro and in vivo. One such category of phytoconstituents is “polysaccharides”. These are found in different parts of the plant such as roots, leaves, stem and leaves and are extracted maximally in polar solvent – for eg. water extract of the afore mentioned plant parts contain the crude polysaccharide fraction. These are non-starch type in nature and can be a mixture of more than one type of polysaccharide too. In this review, an attempt has been made to discuss such therapeutically active plant polysaccharides in terms of their wide pharmacologically active profile along with methods to isolate and characterize them in brief.


Author(s):  
Rupa Sanyal ◽  
◽  
Sohini Mallick ◽  
Asis Mazumder ◽  
◽  
...  

Kripa (Lumnitzera racemosa) is an evergreen branched tree of medicinal value found in the mangrove areas of the Indian subcontinent and traditionally used by local rural communities to treat various ailments and their symptoms. Kripa was identified as one of the many mangrove species that occur in the Sunderbans delta that are being used for its therapeutic properties. The traditional usage of Kripa leaves and bark were learnt through interaction with the locals. The plant parts were collected from the Medicinal Plant Conservation Area (MPCA) in Bonnie Camp and a preliminary phytochemical analysis was conducted in methanolic extraction by following standard methodology. The locals reported that the most common use of the plant were to treat itches, bites (inflammation) and occasionally even symptoms of diabetes. The preliminary phytochemical screening reveals that the leaves of Kripa contains glycosides, alkaloids, phenols, tannins, flavonoids, etc.


2020 ◽  
Vol 16 (3) ◽  
pp. 363-367
Author(s):  
Setiyo Gunawan ◽  
Safrina Hapsari ◽  
Hakun Wirawasista Aparamarta ◽  
Kuswandi Kuswandi ◽  
Raden Darmawan

Calophyllum inophyllum is one of the most notable mangrove species that grows a lot in the coastal areas of the Indonesian archipelago. Despite its long lifespan (50 years) and abundant seed oil production, this plant has not been utilized optimally. Wax is reported as a constituent of C. inophyllum seed oil, but the quantity has not been discovered yet. Wax has been commonly used as ingredient in coating, cosmetic, food, and pharmaceutical industries. The aims of this work were to separate wax from C. inophyllum seed oil in high purity and investigate the effects of crude C. inophyllum to silica gel mass ratio and the stages number on wax isolation. Silica gel was employed to adsorb crude C. inophyllum seed oil. Mass ratios of C. inophyllum seed oil to silica gel used in this work were 1:4, 1:2, and 1:1 (g/g). After that, the seed oil adsorbed onto silica gel was extracted by soxhlet extraction with hexane as the solvent. Wax was separated by putting the hexane extract in cold storage at 4 ºC for 24 h. Furthermore, wax obtained was analyzed by High-Temperature Gas Chromatography (HT-GC) and confirmed by Thin-Layer Chromatography (TLC). It was found that wax (purity 93.2 % and yield 0.4 %) was best isolated by employing two-stage of adsorption-extraction, with crude C. inophyllum seed oil to silica gel mass ratio of 1:1 (g/g), followed by crystallization in cold acetone for 24 h. Moreover, wax content in crude C. inophyllum seed oil was 0.43%.


Author(s):  
Tetiana VORONIUK

Abstract Introduction. The high level of the restaurant business profitability along with its riskiness makes it possible to consider it as a promising sector of development, especially in regions where there are free niches. Significant dynamism of changes in the external environment of this industry in comparison with others necessitates continuous information monitoring. The purpose of the article is to determine the current state of the restaurant industry of Chernivtsi region and to outline perspective directions of its development in the current conditions. Results. The contents of the «restaurant industry» concept and its evolution are considered. It is determined that the modern tendencies of its development are caused by the formation of a market economy with a focus on leisure organization. An dynamics analysis of the restaurant services market in Ukraine is presented. Market analysis of Chernivtsi region has been given. The role of this branch in the region economy is determined. The employment dynamics in the field of temporary accommodation and catering of Chernivtsi region is analyzed, and the forecast of the considered indicator till 2020 is developed. The dynamics analysis of gross value added and output of the research industry for the years 2014-2017 is carried out, which allows to confirm the stable growth, which for the whole period amounted to 41.1% of output and 37.5% of value added. Conclusions. The key development tendencies of the restaurants in the Chernivtsi region are identified: unsaturation of the market and positive trends of growth make it possible to consider it as promising, despite the low population level, trends in employment dynamics make it possible to consider it as a promising source of new jobs, dynamics of output and gross value added testify to the growing demand for restaurant business services, despite the relatively low standard of living. Proposed directions of restaurant market development in Chernivtsi region are offered: price democratization, tourism orientation with preservation of authenticity, deepening of specialization, expansion of entertaining and cognitive services, catering, automation of business processes and online sales. Key words: restaurant industry, restaurant, restaurant market, restaurant business, coffee shop, types of coffee shops, coffee market.


2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Zahra Rahgo ◽  
Hamid reza Samadlouie ◽  
Shideh Mojerlou ◽  
Kambiz Jahanbin

Morchella fungi are considered a good source of protein. The ITS region was used to identify Morchella isolated in the northern region of Iran. The isolated fungus was very similar to Morchella fluvialis. M. fluvialis was first isolated in Iran. Dried biomass of M. fluvialis contained 9% lipids and 50% polysaccharides. Fatty acid profiles of lipids of M. fluvialis are mainly made up of linoleic acid (C18:2) (62%), followed by palmitic acid (C16:0) (12%). Testosterone (TS) was also detected (0.732 ng/dry weight biomass (DWB)) in the hormone profile of this new isolated species. Then, various protein and carbon sources as variable factors were applied to identify the key substrates, which stimulated protein production using the one-factor-at-a-time method. Key substrates (glucose and soybean) were statistically analyzed to determine the optimum content of the protein and DWB accumulation using response surface methods. The highest protein content (38% DWB) was obtained in the medium containing 80 g/l glucose and 40 g/l soybean powder. Total nutritionally indispensable amino acids and conditionally indispensable amino acids constitute 55.7% crude protein. That is to say, these adequate quantities of essential amino acids in the protein of M. fluvialis make it a good and promising source of essential amino acids for human diet.


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