The effectiveness of early local administration of hyaluronic acid in experimental traumatic ischemia of the limb muscles

2021 ◽  
Author(s):  
A.V. Shulepov ◽  
I.A. Shperling ◽  
Yu.V. Yurkevich ◽  
N.V. Shperling
Keyword(s):  
Oxygen Consumption ◽  
Hyaluronic Acid ◽  
Oxygen Saturation ◽  
Wistar Rats ◽  
Soft Tissues ◽  
Local Administration ◽  
Compression Injury ◽  
Doppler Flowmetry ◽  
Muscle Ischemia ◽  
Male Wistar Rats

The experiments were performed on 280 male Wistar rats weighing 300-340 g. The design of the study included: modeling of compression injury of the soft tissues of the thigh, local injection of the preparation of hyaluronic acid "Gialift 3.5" into the area of damage 3 hours after the cessation of compression, taking into account the results after 7, 14 and 28 days. The introduction of hyaluronic acid into the area of compression injury of soft tissues reduces the severity of myoglobinemia, increases the immunohistochemical density of VEGF-positive cells, improves the perfusion characteristics of microcirculation, oxygen saturation, and specific oxygen consumption by tissues. Keywords: traumatic muscle ischemia, hyaluronic acid, myoglobin, microcirculation, metabolism, laser Doppler flowmetry, morphometry.

scholarly journals SANOGENETIC AND PHARMACOLOGICAL EFFECTS OF LOCAL APPLICATION OF HYALURONIC ACID IN EXPERIMENTAL SOFT TISSUE COMPRESSION TRAUMA

2020 ◽  
Vol 10 (2) ◽  
pp. 53-60
Author(s):  
I. A. Shperling ◽  
A. V. Shulepov ◽  
N. V. Shperling ◽  
Yu. V. Yurkevich ◽  
O. Yu. Kuzmina ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Soft Tissue ◽  
Soft Tissues ◽  
Local Application ◽  
Control Group ◽  
Repair System ◽  
Pharmacological Effects ◽  
Muscle Injuries ◽  
Compression Injury ◽  
Tissue Compression

Regional activation of cellular repair systems, including local application of hyaluronic acid, is a promising direction for the sanogenesis of muscle injuries. Purpose: to identify the sanogenetic and pharmacological effects of local application of hyaluronic acid in experimental soft tissue compression trauma. Compression injury was simulated by mechanical compression of the right pelvic limb of a rat at the level of the lower leg for 7 hours with a pressure force of 10-12 kg/cm2. Further, the animals were divided into 2 groups: 3 hours after decompression, animals in the main group were injected with 3.5% hyaluronic acid (the drug «Hyalift 3.5») by fan-pricking with a sterile disposable syringe at 5-6 points to a depth of 0.5-0.8 cm in a volume of up to 0.1 ml at each point; rats in the control group were given a similar method and an equivalent volume of 0.9% sodium chloride solution. The level of myoglobin in the blood, microcirculation, histomorphological and immuno- histochemical characteristics of tissues in the compression area were studied. A comprehensive analysis of the results of the study allowed us to establish that early local administration of 3.5% hyaluronic acid to the area of compression damage of soft tissues (3 hours after decompression) has a muscle-protecting effect, activates the repair system of damaged skeletal muscles. It is concluded that the sanogenetic effect of hyaluronic acid is mediated by the mecha- nisms of its pharmacological action: activation of angiogenesis, improvement of in situ efficiency of microcirculation and metabolism, reduction of the severity of primary and secondary necrosis and about traumatic disorders, activation of regeneration of damaged muscle fibers with the formation of muscle tissue. Hyaluronic acid is an active stimulant of reparative processes in the perspective of pharmacotherapy and may be a promising component in the local correction of extensive traumatic injuries.

scholarly journals Analysis of microcirculatory disorders in men with more than 10 years’ experience on night work

2021 ◽  
Vol 102 (4) ◽  
pp. 474-478
Author(s):  
I A Gribanov ◽  
E G Zarubina
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Saturation ◽  
Healthy Volunteers ◽  
Work Experience ◽  
Oxygen Exchange ◽  
Night Work ◽  
Control Group ◽  
Doppler Flowmetry ◽  
Arterial Blood

Aim. To study the nature and extent of the effects of long-term sleep and wake phase disorders (working at night for more than 10 years) on the characteristics of microcirculation in men. Methods. Laser Doppler flowmetry and spectophotometry, optical tissue oximetry, and laser-induced fluorescence spectroscopy were performed in 34 male patients with a mean age of 40.30.9 years and more than 10 years experience of night work to assess the effectiveness of microcirculation. 25 men with a mean age of 40.21.2 without night work experience were used as a control group. Microcirculation was studied on the laser diagnostic complex LAKK-M (LAZMA, Russian Federation). The measurement was carried out on the skin of the palmar surface of the terminal phalanx of the second finger of the hand. The following indicators were analyzed in automatic mode: the mean perfusion value, the index of specific oxygen consumption in the tissue, capillary blood saturation, the relative volume of the red blood cell fraction, arterial blood oxygen saturation, the index of perfusion oxygen saturation in the microcirculation, the efficiency of oxygen exchange and the fluorescent indicator of oxygen consumption. Statistical processing of the results was carried out using the Student's t-test for independent samples. Results. In patients working at night for more than 10 years, a decrease in microcirculation efficiency was revealed. The index of specific oxygen consumption in the tissue was lower than that of healthy volunteers by 34.1% (p=0.000255), and the index of oxygen exchange efficiency by 56.3% (p 0.001). Long-term night work (10 years) can lead to violations of microcirculation parameters and an irreversible decrease in the efficiency of oxygen exchange compared with the control group by an average of 56.3% (18.00.5 for the group with night work experience, 41.20.6 for the group of healthy volunteers, p 0.001), the index of specific oxygen consumption in the tissue by an average of 34.1% (1.530.03 for the group with night work experience, 2.320.2 for the group of healthy volunteers, p=0.000255) and an increase in the index of perfusion oxygen saturation in the microcirculation by 2 times compared with the control group (6.20.05 for the group with night work experience, 3.670.09 for the group of healthy volunteers, p 0.001), which is accompanied by an increase in the saturation of mixed (and venous) blood. Conclusion. The nature of the revealed violations of microcirculatory parameters in the long-term night work suggests their significance in the development of diseases that are currently attributable to the so-called group of diseases of civilization.

scholarly journals Maximum oxygen consumption and quantification of exercise intensity in untrained male Wistar rats

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Fei Qin ◽  
Yanan Dong ◽  
Songtao Wang ◽  
Minxiao Xu ◽  
Zhongwei Wang ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Exercise Intensity ◽  
Wistar Rats ◽  
Maximum Oxygen Consumption ◽  
Male Wistar Rats

Some features of organism response on local administration of reactive oxygen

2015 ◽  
Vol 9 (4) ◽  
pp. 0-0
Author(s):  
Перетягин ◽  
P. Peretyagin ◽  
Ковалева ◽  
L. Kovaleva ◽  
Перетягин ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Wistar Rats ◽  
Reactive Oxygen ◽  
Local Administration ◽  
Oxygen Species ◽  
Animal Tissues ◽  
Local Effects ◽  
Doppler Flowmetry ◽  
Fourth Group ◽  
Made In

The purpose of this work is the study of microcirculation state at different local effects of reactive oxygen species on animal tissues. The experiment was performed on 60 healthy white Wistar rats. Control group (without any manipulations) included 20 Wistar rats. Also 4 main groups were formed (10 rats in each). Rats of the first group (n=10) were intracutaneously injected with 1 ml of oxygen (in the back), a local darsonvalization during 4 min. was carried out in animals of the second group (n=10). In third group (n=10) the injection of 1 ml of oxygen-ozone mixture was made, in the fourth group (n=10) - darsonvalization during 4 min. after a preliminary introduction of oxygen (1 ml). Microcirculation parameters were tested by laser Doppler flowmetry with LAKK-02 device. It was defined, that local effects of reactive oxygen species include microcirculation changes, depending from specialties of exposed factor.

FACTORS IN THE REDUCED SURVIVAL TIME DURING STARVATION IN THE COLD OF RATS PREVIOUSLY FED A LOW-PROTEIN DIET

1964 ◽  
Vol 42 (4) ◽  
pp. 533-545 ◽  
Author(s):  
J. R. Beaton ◽  
V. Feleki ◽  
J. A. F. Stevenson
Keyword(s):  
Oxygen Consumption ◽  
Food Consumption ◽  
Water Loss ◽  
Wistar Rats ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Sprague Dawley ◽  
Low Protein ◽  
Sprague Dawley Rats ◽  
Male Wistar Rats

Male Wistar rats fed a low-protein diet (5% by weight) did not withstand subsequent deprivation of food at 5 °C or 23 °C as long as controls that had been fed a 20% protein diet isocalorically. This was also true in Sprague–Dawley rats which were studied only at 5 °C. This effect of the low-protein diet was not attributable to differences in food consumption, or in "resting" oxygen consumption. The rats fed low-protein had somewhat less fat when deprived of food but, perhaps more importantly, appeared to use less of their reserves of energy during food deprivation. Apparent water loss and thus dehydration appeared to be greater in these animals.

Structural and functional renal alterations in five-sixth nephrectomized rats on unrestricted diet

1986 ◽  
Vol 44 ◽  
pp. 342-343
Author(s):  
I. Stachura ◽  
M. Pardo ◽  
J. Costello ◽  
D.M. Landwehr
Keyword(s):  
Wistar Rats ◽  
Renal Damage ◽  
Standard Diet ◽  
Experimental Conditions ◽  
Phosphate Intake ◽  
Dietary Restrictions ◽  
Severe Reduction ◽  
Male Wistar Rats ◽  
Unrestricted Diet ◽  
Progressive Renal Insufficiency

Under experimental conditions severe reduction of renal mass results in the hyperfiltration of the remaining nephrons leading to a progressive renal insufficiency. Similar changes are observed in patients with various renal disorders associated with a loss of the functioning nephrons. The progression of renal damage is accelerated by high protein and phosphate intake, and may be modified by the dietary restrictions.We studied 50 five-sixth nephrectarrized male Wistar rats on a standard diet (Rodent Laboratory Chow 5001 Ralston Purina Co., Richmond, Indiana; containing 23.4% protein) over a 20 week period.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

Augurin stimulates food intake in male Wistar rats

2013 ◽  
pp. 1-1
Author(s):  
Michael Patterson ◽  
John Tadross ◽  
Keisuke Suzuki ◽  
Kylie Beale ◽  
Charoltte Boughton ◽  
...  
Keyword(s):  
Food Intake ◽  
Wistar Rats ◽  
Male Wistar Rats
Sign in / Sign up

Export Citation Format

Share Document