scholarly journals Short-term exercise training increases plasma levels of klotho and total antioxidant capacity in male Wistar rats

2019 ◽  
Vol 21 (1) ◽  
pp. 25-30
Author(s):  
Maral Ramez ◽  
Farinaz Nasirinezhad ◽  
Hamid Rajabi ◽  
Nahid Aboutaleb ◽  
Nasim Naderi
Keyword(s):  
Exercise Training ◽  
Antioxidant Capacity ◽  
Plasma Levels ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Moderate Intensity ◽  
Short Term ◽  
Vo2 Max ◽  
Total Antioxidant ◽  
Male Wistar Rats

Background and aims: Soluble or circulating form of Klotho (i.e., anti-aging and longevity protein) has biological effects on various body cells and tissues. Considering the role of exercise training on protective proteins, the present study aimed to investigate and compare the effects of short-term high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on plasma levels of Klotho, total antioxidant capacity (TAC), and malondialdehyde (MDA). Methods: In this study, 24 male Wistar rats with a weight range of 250-300 g and 8-10 weeks old were randomly divided into control, HIIT, and MICT groups. Training included five consecutive days on the treadmill. HIIT including 6×2 minutes high intermittence with 85%-90% Vo2 max and 5×2 minutes slow intermittence (active recovery) with 55%-60% Vo2 max. MICT program was performed at 70% Vo2 max that total distance running was the same for the HIIT group. Then, the plasma levels of Klotho, TAC, and MDA were measured by ELISA assay. Finally, one-way ANOVA and post-hoc Tukey tests were used for statistical analysis. Results: Based on the results, a significant increase was observed in plasma levels of Klotho and TAC in both HIIT and MICT groups compared to the control group (P<0.001) while these changes were significantly more dominant in the HIIT group compared to MICT group (P=0.024). However, the results demonstrated no significant difference between the groups regarding MDA (P=0.109). Contrarily, the finding revealed a significant positive correlation between Klotho and TAC (r=0.79, 0.83, 0.79 in control, HIIT, and MICT groups, respectively, P<0.05), whereas a significant negative association between Klotho with MDA within all the groups (r= -0.78, - 0.86, and -0.81). Conclusion: In general, even short-term aerobic exercise training, especially HIIT increases circulating Klotho and TAC, therefore, this can confirm the positive effects of the training-induced exercise.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

scholarly journals Short-term Effects of Composted Cattle Manure or Cotton Burr on Growth, Physiology, and Phytochemical of Spinach

HortScience ◽  
2016 ◽  
Vol 51 (12) ◽  
pp. 1517-1523
Author(s):  
Chenping Xu ◽  
Beiquan Mou
Keyword(s):  
Amino Acid ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Spinacia Oleracea ◽  
Cattle Manure ◽  
Total Phenolic ◽  
Short Term ◽  
Growth Physiology ◽  
Compost Amendment ◽  
Total Antioxidant

Compost is increasingly used in horticultural crop production as soil conditioner and fertilizer because of its contribution to agriculture sustainability. The short-term (35 days after transplanting) effects of composted cattle manure or cotton burr on growth, physiology, and phytochemical of spinach (Spinacia oleracea L.) were evaluated in a greenhouse. Composted cattle manure at 5% or 10% mix rate (5Ca or 10Ca) greatly enhanced spinach growth as indicated by increased leaf number, area, fresh and dry weights (FW and DW), shoot FW and DW, and root DW. They also increased water use efficiency (WUE) and shoot:root ratio, and improved the photochemistry of mature leaves. Chlorophyll content also increased under 10Ca treatment. Composted cotton burr also improved spinach growth but only at 10% amendments (10Co), and was less efficient than composted cattle manure. Specific leaf area (SLA) decreased and succulence increased under all compost amendment indicating that compost could improve spinach quality. All soil amendments reduced the content of total phenolic and anthocyanin, while only 10Co and 5Ca treatments decreased flavonoid content and total antioxidant capacity. The content of carotenoid and protein increased in 10Ca treatment and amino acid content increased under both 5Ca and 10Ca treatments. The results indicated that compost, especially composted cattle manure mixed at 10%, improved spinach production and quality, and with proper application rate enhanced nutritional value by increasing carotenoid, protein, and amino acid contents while having little effect on total antioxidant capacity.

scholarly journals Effects of Exercise Training Intensity on Plasma Levels of Creatinine Kinas After a Myocardial Infarction in Male Wistar Rats

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Abdolhamid Zokaei ◽  
Mehran Ghahramani
Keyword(s):  
Myocardial Infarction ◽  
Exercise Training ◽  
Plasma Levels ◽  
Wistar Rats ◽  
Interval Training ◽  
Cardiac Cells ◽  
Repeated Measure ◽  
Control Group ◽  
Male Wistar Rats ◽  
Intensity Of Exercise

Background: The creatine phosphokinase-MB (CPK-MB) isoenzyme level is useful in determining the myocardial infarction (MI) extent and time. Evidence indicates the possible effect of exercise training on reducing the amount of CPK-MB, but the proper intensity of exercise is still unclear. Objectives: In this study the effect of intensity of exercise training on creatinine kinas after a myocardial infarction in plasma levels of male Wistar rats was comparing. Methods: Thirty two male Wistar rats at 10 weeks of age were divided in to four groups [low (N = 8), moderate (N = 8), and high (N = 8) intensity interval training and a control (N = 8) group (no exercise)]. Six weeks later, rats became MI patients through surgery. CPK-MB plasma levels were investigated before training, immediately after training for six weeks as well as 12 hours after MI. The data were analyzed by ANOVA with repeated measure and Tukey (α ≥ 0.05). Results: The results showed that changes in CK enzyme at 12 hours after MI in LIIT (113.88 ± 19.25), MIIT (135.88 ± 35.58) and HIIT (105.75 ± 19.05) were statistically significant compared to the control group (124.62 ± 23.41) only in 12 hours after MI and this reduction in the low-intensity interval training group was significant than the other two groups (P = 0.0001). Conclusions: In this study, six weeks of interval training with each intensity was likely to make cardiac cells resistant to necrosis resulting in low levels of the CPK-MB enzyme compared to the control group after the myocardial infarction event.

scholarly journals Beneficial Effects of Apple Vinegar on Hyperglycemia and Hyperlipidemia in Hypercaloric-Fed Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Driss Ousaaid ◽  
Hassan Laaroussi ◽  
Meryem Bakour ◽  
Asmae ElGhouizi ◽  
Abderrazak Aboulghazi ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Lipid Profile ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Radical Scavenging ◽  
Total Acidity ◽  
Scavenging Activity ◽  
Total Antioxidant ◽  
Hypercaloric Diet

The main objective of this study is to determine the effects of apple vinegar on the metabolic changes caused by hypercaloric diet in Wistar rats. Apple vinegar was first analyzed to find out the total acidity, the polyphenolic and flavonoid contents, the total antioxidant capacity, and the free radical scavenging activity. In vivo study on adult male and female Wistar rats was conducted by administering a drink containing either 10% D-glucose or water (control) for five weeks. Apple vinegar is administered daily by gavage (2 mL/kg) to rats fed D-glucose for 5 weeks. The results showed that the polyphenolic content in apple vinegar was 148.02±10.16 mg GAE/100 mL, flavonoid content was 22.93±0.73 QE/100 mL, and total antioxidant capacity was 13.4±0.47 mg AAE/100 mL. Free radical IC50 apple vinegar scavenging activity (DPPH) was 0.74±0.154 μL/mL. The total acidity was (3.24±0.02 mg AAE/100 mL). The treatment during five weeks with D-glucose leads to increased plasma glucose, lipid profile, hepatic enzyme levels, urea, and creatinine. Simultaneous treatment with apple vinegar improves the parameters studied. These results clearly show that the daily consumption of vinegar can reduce the rise in blood sugar and lipid profile induced by hypercaloric diet in rats. Therefore, the use of apple vinegar would have a very beneficial effect in the prevention of metabolic disorders caused by high-caloric food.

scholarly journals Acute moderate-intensity exercise increases total antioxidant capacity and anti-inflammatory responses in competitive cyclists: The role of adiponectin

2021 ◽  
Vol 19 ◽  
pp. 205873922199889
Author(s):  
Tadeja Jakus ◽  
Mihaela Jurdana ◽  
Lovro Žiberna ◽  
Zala J Pražnikar
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Inflammatory Markers ◽  
Inflammatory Responses ◽  
The Body ◽  
Moderate Intensity ◽  
Moderate Intensity Exercise ◽  
Total Antioxidant ◽  
Competitive Cyclists ◽  
Anti Inflammatory

High-intensity exercise can elicit acute changes in the biochemical and physiological processes in the body of an athlete, including increased oxidative stress and inflammation. The purpose of this study was to explore the effect of acute moderate-intensity exercise on total antioxidant capacity (TAC) and serum levels of anti-inflammatory adiponectin (APN), and inflammatory markers in competitive cyclists. Ten male cyclists (age 15–26 years, body mass index 19.4–24.7 kg/m2) participated in this study. Each subject performed the maximal oxygen uptake test (VO2peak) and completed a 10-min cycling exercise at a workload of 50% of the peak of VO2peak. Blood samples were collected on three different occasions: after an overnight fasting and at the exercise workloads of 50% and 100% VO2peak. We measured APN, TAC, inflammatory markers as well as assessed nutrient and energy intake for each participant. Baseline concentration of serum APN (10.92 µg/mL) significantly increased at 50% and at 100% VO2peak. In addition, TAC also increased after acute exercise (0.079 vs 0.093 nmol/µL). The concentration of APN at 50% VO2peak positively correlated with the CRP ( r = 0.640, p = 0.046) and negatively correlated with TNF-α ( r = −0.696, p = 0.025). This test showed that short (10 min) and medium-intensity (50% VO2peak) exercise activity in trained athletes evoked beneficial antioxidant and anti-inflammatory responses. Importantly, this response correlates with the increase in APN levels thereby showing that highly trained individuals have beneficial responses originating from adipose tissue. Our observations show that a short training at moderate activity can be an important preservative strategy during the recovery training period.

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