scholarly journals Comparison of Momordica charantia effect on Mcl-1 gene expression in the livers of streptozotocin diabetic and healthy rats

2019 ◽  
Vol 21 (6) ◽  
pp. 265-270
Author(s):  
Mahsa Salehian-Dehkordi ◽  
Hossein Sazegar
Keyword(s):  
Gene Expression ◽  
Statistical Tests ◽  
Diabetic Control ◽  
Momordica Charantia ◽  
Control Group ◽  
Positive Effects ◽  
Diabetic Control Group ◽  
Male Wistar Rats ◽  
Healthy Control ◽  
Group B

Background and aims: The positive effects of medicinal herbs on diabetes have been proved in previous studies. The aim of the present study was to evaluate the effect of active Momordica Charantia on the treatment of liver diseases resulting from diabetes and the expression level of the Mcl-1 gene, which is a proapoptotic gene and becomes antiapoptotic in the event of damage. Methods: In this study, 42 adult male Wistar rats were randomly divided into 7 groups including healthy, diabetic, metformin, 150 mg/kg M. charantia controls, and three groups that received the active M. charantia with doses of 50, 100, and 150 mg/kg. All groups became diabetic with streptozotocin injected intraperitoneally except for the control and M. charantia. Afterward, they received the active M. charantia by gavage for four weeks (three times a week). Finally, the Kruskal-Wallis method was used for comparison among the groups. The statistical tests were analyzed using SPSS software, version 22. Results: The level of Mcl-1 expression in the diabetic control group (C) was significantly higher than that in the healthy control (A) and the M. charantia-receiving control group (B, P<0.05). The group receiving 150 mg/kg dose of M. charantia drug (G) had a better effect compared to the group that received 100 mg/kg (F), and this difference was significant (P<0.05). This increase indicated that the medication was dose-dependent. Conclusion: In general, a reduction in the level of Mcl-1 gene expression relied on the M. charantia dose. After the development of diabetes, this level significantly increased in the diabetic groups, but decreased after receiving M. charantia, leading to a decrease in the side effects and symptoms associated with diabetes.

scholarly journals Effects of Silymarin on Blood Glucose Concentration, Hepatic Histopathological Changes and FOXA2 and FOXA3 Gene Expression in Streptozotocin-Induced Diabetic Male Wistar Rats

2021 ◽  
Vol 5 (2) ◽  
pp. 17
Author(s):  
Sajad Nikkhah ◽  
Rahman Jafari Hafshejan ◽  
Farshid Gheibi Hajivar ◽  
Khalil Khashei ◽  
Sara Afzali
Keyword(s):  
Gene Expression ◽  
Blood Glucose ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Histopathological Changes ◽  
Diabetic Control Group ◽  
Male Wistar Rats

Since the liver is among the primary organs susceptible to the effects of hyperglycaemia, diabetes mellitus (DM) could be a risk factor for the development and progression of liver damage. In present study, since no side-effects from the herbal medicine have been reported, the effect of silymarin on blood glucose concentration, hepatic histopathological changes and FOXA2 and FOXA3 gene expression, which are key genes in liver regeneration, was investigated. In this fundamental with experimental approach study, 40 male Wistar rats weighing 180-220 g were used. Rats were kept under the standard conditions of temperature of 20-22°C and humidity of 50% and consecutive 12-hour periods of light and darkness. Rats were randomly divided into five different groups (n=8 each), including healthy control rats, diabetic control rats, diabetic rats receiving silymarin (50, 100 and 150 mg/kg). Diabetes was induced by injecting streptozotocin (50 mg/kg B.W., i.p.). For 4 weeks silymarin groups received the drug once every three days through gavage and fasting blood glucose concentration measured once every 10 days. At the end of a month experiment, livers were harvested for hepatic histopathological and FOXA2 and FOXA3 gene expression changes analysis. In the diabetic rats treated with silymarin (50, 100 and 150 mg/kg), by comparison with the diabetic control group (p<0.05), glucose levels decreased significantly. Moreover, FOXA2 and FOXA3 expression in diabetic groups treated with silymarin significantly increased compared to diabetic control group (p<0.05). Hepatic histopathological changes were improved in the treated groups.The present study indicates that silymarin significantly decreased blood glucose concentration and increased the FOXA2 and FOXA3 gene products level. Hence, silymarin is able to improve some of the symptoms associated with diabetes and possesses hepatoprotective effects in streptozotocin-induced diabetic rats.

scholarly journals Effect of Eight Weeks of Endurance Training and Stevia Supplemention on Atrial Natriuretic Peptide and β-Myosin Heavy Chain Expression Levels in Heart Tissue of Rats With Type 1 Diabetes

2020 ◽  
Vol 24 (4) ◽  
pp. 308-319
Author(s):  
Kobra Soleymani ◽  
◽  
Asieh Abbassi Daloii ◽  
Ali Reza Barari ◽  
Ayoub Saeidi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endurance Training ◽  
Cardiac Tissue ◽  
Diabetic Control ◽  
Heart Tissue ◽  
Control Group ◽  
Expression Levels ◽  
Diabetic Control Group ◽  
Healthy Control

Background: The effects of exercise and stevia extract on diabetes-related indicators have been already reported, but their cardiac benefits on Type 1 Diabetes (T1D) are unclear. Objective: This study aims to evaluate the effect of eight weeks of endurance training and stevia supplementation on gene expression levels of Atrial Natriuretic Peptide (ANP) and β-Myosin Heavy Chain (β-MHC) in the heart tissue of T1D rats. Methods: In this experimental study, 25 rats with the average weight of 250-300 g were divided into five groups; healthy control, diabetic control, diabetic+supplementation, diabetic+training, and diabetic+training+supplementation. T1D was induced by intraperitoneal injection of streptozotocin (55 mg/kg/ body weight). Endurance training was performed 5 days a week at a speed of 20-30 meters per minute on a surface with a zero slope for 8 weeks. Stevia was gavaged in a dose of 250 mg/kg/body weight. Rats were slaughtered 48 hours after the last training session. Cardiac tissue was used to measure the parameters. The gene expression of ANP and b-MHC in cardiac tissue was measured by real-time Polymerase Chain Reaction (PCR) method. Data were analyzed by using one-way ANOVA and Bonferroni post hoc test. Findings: The gene expression levels of ANP and β-MHC were significantly higher in the diabetic control group compared to the healthy control group (P=0.001), and significantly lower in the diabetic+training and diabetic+training+supplementation groups compared to the diabetic control group (P=0.001). Conclusion: Endurance training and stevia supplementation can have beneficial effects on the heart of T1D rats.

scholarly journals Comparative study of the effect of ethanolic extract of swietenia mahagoni seeds with rosiglitazone on experimentally induced diabetes mellitus in rats

2013 ◽  
Vol 39 (1) ◽  
pp. 6-10
Author(s):  
SMMMA Hasan ◽  
MI Khan ◽  
BU Kumar ◽  
MZ Sadeque
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Control Group ◽  
Group B ◽  
Group D ◽  
Swietenia Mahagoni ◽  
Experimentally Induced

The study was performed to compare the blood glucose lowering effect of Swietenia mahagoni seeds with an oral antidiabetic drug, rosiglitazone in experimentally induced diabetic rats. Twentyfour healthy Long Evans Norwegian strain of rats were included in the study and divided into four groups (A, B, C and D) comprising 6 rats each. Group A (control group) received standard rat food for 14 days. Diabetes was induced by a single intraperitoneal administration of alloxan 120mg/kg body weight in Group B, C and D. Group B was given standard food for 10 days and considered as diabetic control. Group C and D were treated with ethanolic extract of Swietenia mahagoni seeds 1000mg/kg and rosiglitazone 10mg/kg orally respectively. Administration of ethanolic extract of Swietenia mahagoni seeds in group C and rosiglitazone in group D produced a significant reduction in blood glucose level as compared to diabetic control (group B). Histological examination of pancreas showed destruction of beta cells in Islets of pancreas in group B whereas retaining of islets and few degranulations of beta cells of pancreas found in group C and group D. The observations and results of the present study provide information that ethanolic extract of Swietenia mahagoni seeds has hypoglycaemic effect in experimentally induced diabetic rats which requires further investigation. DOI: http://dx.doi.org/10.3329/bmrcb.v39i1.15790 Bangladesh Med Res Counc Bull 2013; 39: 6-10

scholarly journals The Protective Effect of Marigold Hydroalcoholic Extract in STZ-Induced Diabetic Rats: Evaluation of Cardiac and Pancreatic Biomarkers in the Serum

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Esmaeel Ebrahimi ◽  
Saeed Shirali ◽  
Rahman Talaei
Keyword(s):  
Diabetes Mellitus ◽  
Protective Effect ◽  
Antioxidant Property ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Dependent Manner ◽  
Hydroalcoholic Extract ◽  
Diabetic Control Group ◽  
Male Wistar Rats

Diabetes mellitus is the most common endocrine disorder worldwide and it is usually along with complications such as retinopathy, neuropathy, nephropathy, and cardiovascular disease. The prevalence of diabetes is increasing and its treatment has created concerns in society. The use of herbal medicine can be helpful in the management of diabetes mellitus. The aim of this study was to investigate the protective effect of marigold hydroalcoholic extract under diabetes condition. A total of 36 male Wistar rats were randomly divided into four groups: normal control group, diabetic control group, and groups treated with 250 or 500 mg/kg hydroalcoholic extract of marigold flower during four weeks. At the end of the study, the rats were anesthetized with ketamine/xylazine, and sampling was performed through cardiac puncture. The results showed that treatment with marigold improved body weight. In addition, we determined that marigold normalized the level of CK-MB, total CK, amylase, and lipase in a dose-dependent manner. Probably these effects resulted from antioxidant property of marigold; thus we suggest that marigold flower can be useful for reduction of diabetes complication.

scholarly journals Effect of Aerobic Exercise Combined With Stevia Extract Supplementation on Selected Liver Enzymes in Diabetic Rats

2020 ◽  
Vol 10 (3) ◽  
pp. 244-257
Author(s):  
Abdollah Akbari ◽  
◽  
Vahid Tadibi ◽  
Naser Behpoor ◽  
◽  
...  
Keyword(s):  
Aerobic Exercise ◽  
Liver Enzymes ◽  
Exercise Program ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Control Group ◽  
Wide Range ◽  
Healthy Control ◽  
Post Hoc

Objective: Diabetes is a chronic disease associated with a wide range of liver diseases. The levels of liver enzymes such as Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) are changed following a liver disease The purpose of this study is to investigate the effect of aerobic exercise combined with Stevia extract supplementation on the levels of ALT and AST enzymes in diabetic rats. Methods: In this experimental study, 40 males wistar rats weighing 150-220 g were used. Animals were randomly divided into five groups; healthy control , diabetic control, diabetic + exercise, diabetic + stevia, and diabetic + exercise + stevia. The extract was administered by oral gavage for 6 weeks. The aerobic exercise program was performed for six weeks, 5 days per week. At the end of the intervention, blood samples were collected from the heart of rats to measure the levels of ALT and AST. Data were analyzed by one-way ANOVA and Tukey’s post hoc test. Results: The levels of ALT and AST enzymes decreased significantly in all experimental groups compared to diabetic control group (P<0.05). Conclusion: Aerobic exercise combined with stevia extract supplementation can improve the liver function in streptozotocin-induced diabetic rats

scholarly journals Effect of Fenugreek (Methi) on the Thymic volume in Streptozotocin-induced Diabetic Rats

1970 ◽  
Vol 16 (1) ◽  
pp. 1-4
Author(s):  
DK Mondal ◽  
MM Karim ◽  
S Mohal ◽  
BMA Yousuf ◽  
KM Shamim
Keyword(s):  
Body Weight ◽  
Treated Group ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Control Group ◽  
Final Body Weight ◽  
Diabetic Control Group ◽  
Group A ◽  
Group B

Effect of fenugreek (Trigonella foenumgraecum) on thymic volume was studied in 30 long Evansrats. Among the total, 10 were treated with only vehicle (non-diabetic control - group A); 10 weretreated with streptozotocin only (diabetic control - group B) and 10 were treated with fenugreek(fenugreek treated - group C). Mean relative thymic volumes were 0.22210.018 ml/100 gm ofcorresponding final body weight (Mean 1 SEM), 0.14710.015 and 0.19910.020 in group A, B and Crespectively. In non-diabetic control group the thymic volume was significantly higher (p=0.005)than that of diabetic control group. In fenugreek treated diabetic group the volume was alsosignificantly higher (p=0.05) from that of diabetic control group and very much nearer to nondiabeticcontrol group (p=0.398). Fenugreek may preserve the thymic volume considerably indiabetic rats.doi: 10.3329/taj.v16i1.3892TAJ June 2003; Vol.16(1): 1-4

scholarly journals Effect of ropivacaine on peripheral neuropathy in streptozocin diabetes-induced rats through TRPV1-CGRP pathway

2019 ◽  
Vol 39 (11) ◽  
Author(s):  
Nanwen Zhang ◽  
Haixiang Wei ◽  
Weifang Wu ◽  
Peimin Lin ◽  
Yuan Chen ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Peripheral Neuropathy ◽  
Conduction Velocity ◽  
Nerve Conduction Velocity ◽  
Diabetic Control ◽  
Control Group ◽  
Diabetic Control Group ◽  
Nondiabetic Control ◽  
Group A ◽  
Group B

Abstract Objective To determine the effect of ropivacaine on peripheral neuropathy in diabetic rats and its possible mechanism. Methods Forty-eight Sprague–Dawley rats were randomly divided into six groups: nondiabetic control group, nondiabetic group A (0.25% ropivacaine), nondiabetic group B (0.75% ropivacaine), diabetic control group (diabetic peripheral neuropathy (DPN) +artificial cerebrospinal fluid), diabetic group A (DPN+0.25% ropivacaine), and diabetic group B (DPN + 0.75% ropivacaine), with eight rats in each group. Within an hour of the last administration, the sciatic motor nerve conduction velocity (MNCV) of each group was measured, and the morphological changes of rat sciatic nerve were observed by HE, Weil’s staining and electron microscopy. The expression of transient receptor potential vanilloid (TRPV1) in the spinal cord dorsal horn of rats was analyzed by immunohistochemistry, and the expression of Calcitonin gene-related peptide (CGRP) protein in the spinal cord was analyzed by Western blot. Results Compared with the nondiabetic control group, elevated blood glucose, decreased weight and reduced average mechanical withdrawal threshold (MWT), additionally, the sciatic nerves showed significantly slowed conduction velocity (both P<0.001) and damaged pathological structure, the expression of TRPV1 and CGRP were decreased (both P<0.001) in the diabetic groups. Compared with the diabetic control group, down-regulation of TRPV1 and CGRP in spinal cord was significant for the diabetic groups A and B treated with 0.25 and 0.75% ropivacaine, the higher concentration of ropivacaine correlated with a greater change. Conclusion Ropivacaine can significantly block sciatic nerve conduction velocity in DPN rats in a concentration-dependent manner, which may be related to the expression of the TRPV1-CGRP pathway.

scholarly journals Antidiabetic effect of bitter melon/Kerala (Momordica charantia) in alloxan induced diabetic rat

2018 ◽  
Vol 5 (3) ◽  
pp. 373-379
Author(s):  
Rupaly Akhter ◽  
Imrul Hasan Rasel ◽  
Mohammad Saiful Islam
Keyword(s):  
Body Weight ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Momordica Charantia ◽  
Lipoprotein Cholesterol ◽  
Diabetic Rat ◽  
Control Group ◽  
Bitter Melon ◽  
Group B

This study aims at investigating the effect of Momordica charantia extract on glucose tolerance and some biochemical parameters in alloxan induced diabetes rat. A total of 150 rats (50 normal rats and 100 alloxan induced diabetic rats) were used for five trials. The rats were divided into three groups for each trial, each containing 10 individuals as follows: Group A: is the control group, Group B as diabetic control group and group C were diabetic rat received bitter melon (Momordica charantia). Then alloxan injection was injected at a dose rate of 150 mg/kg body weight through intra-peritoneal route to each rat to induce diabetes in groups B and C. Aqueous extract of bitter melon (Momordica charantia) were fed by gavage at a dose of 300 mg/kg body weight daily for 21 days in group C. On 15th day blood glucose level and the body weights, biochemical parameters were measured for the first time to ensure diabetic induction. Then all the rats of this group were kept for more 21 days for the treatment of diabetic condition. Alloxan produced a significant increase in serum glucose, triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), Alanine aminotransaminase (ALT) and Aspartate aminotransaminase (AST) and significant decrease in body weight and high density lipoprotein cholesterol (HDLC). Bitter melon treatment reduced non-fasting and fasting glucose level (p<0.05). In addition, administration of bitter melon juice was associated with a reduction in the serum levels of Alanine aminotransaminase (ALT) and Aspartate aminotransaminase (AST), Triglyceride (TG), Total Cholesterol (TC) and Low Density Lipoprotein Cholesterol (LDL-C) when compared with positive diabetic control (p<0.05). High density lipoprotein cholesterol (HDLC) and body weight level significantly increased in bitter melon treated group C compare to Group B (p<0.05). Res. Agric., Livest. Fish.5(3): 373-379, December 2018

scholarly journals Comparative Study of the Effect of Ethanol Extract of Psidium Guajava Linn Leaves with Glibenclamide on Experimentally Induced Diabetes Mellitus in Rats

2017 ◽  
Vol 5 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Azmary Momtaz ◽  
Khaleda Sharmin ◽  
Sharmin Rahman ◽  
Nashid Sultana ◽  
Rayhana Sharmin
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Ethanol Extract ◽  
Psidium Guajava ◽  
Diabetic Control ◽  
Control Group ◽  
Diabetic Control Group ◽  
Group A ◽  
Group B ◽  
Experimentally Induced

Background: Diabetes Mellitus is a major health problem not only in urban but also in the rural areas of Bangladesh. Regarding its treatment, a suitable drug is yet to be available which can permanently cure this disease. Over 400 traditional plants have been reported for the treatment of diabetes, but only a small number have received scientific and medical evaluation to assess their efficacy.Objective: The study was performed to compare the anti diabetic effect of Psidium guajava Linn leaves with an oral anti diabetic drug (Glibenclamide), in experimentally induced diabetic rats.Materials and method: The experiment was carried out in the department of Pharmacology & Therapeutics of Dhaka Medical College, Dhaka, Bangladesh from July 2012 to June 2013. Twenty four healthy rats of Long Evans Norwegian strain were divided into 4 groups (group A, B, C and D) comprising 6 rats in each. Group A (normal control) received standard rat food for 14 days. Diabetes was induced by administration of Alloxan 120 mg/kg/body weight in group B, C and D. Group B (diabetic control group) was given standard rat food. Group C and D was treated with ethanol extract of Psidium guajava leaves 100 mg/kg body weight and Glibenclamide 1.5 mg/kg/day orally respectively. Total duration of the experiment was 15 days.Results: Administration of ethanol extract of Psidium guajava leaves in group C and Glibenclamide in group D produced a significant reduction (p<0.001) in blood glucose level as compared to group B (diabetic control group).Conclusion: Ethanol extract of Psidium guajava leaves can be considered as a potential anti diabetic agent like Glibenclamide but it requires further investigations.Delta Med Col J. Jan 2017 5(1): 9-14

scholarly journals Beneficial Hypoglycemic, Hypolipidemic Effects of Aerial Branches and Roots Extract of Eryngium caeruleum M. Bieb on Streptozotocin-Induced Diabetes Model in Rats

2021 ◽  
Author(s):  
Aryaneh Sardarbandeh ◽  
Mohammad Reza Delnavazi ◽  
Mohammad Sharifzadeh ◽  
Mohammad Sharifzadeh ◽  
Masoumeh Ghajarieh Sepanlou ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Lipid ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Animal Blood ◽  
Hydroalcoholic Extract ◽  
Diabetic Control Group ◽  
Diabetes Model ◽  
Male Wistar Rats

Eryngium caeruleum (Apiacea) is native to the northern forests of Iran. The anti-diabetic effect of other species of the genus Eryngium has already been reported in previous studies. In this study, the anti-diabetic effect of this extract on animal blood lipid factors was investigated. Hydroalcoholic extract was obtained from different parts of the plant, including roots, leaves, and aerial branches with fruits were prepared by maceration with 70% ethanol. Oral acute toxicity of the extracts was assayed in different doses of 2000, 4000, and 8000 mg/kg in rats. To induce diabetes in the studied animals, 60-70 mg/kg of streptozotocin (STZ) was injected intraperitoneally (IP). For the purpose of this study, 72 male Wistar rats were randomly divided into different groups of normal, diabetic, and positive controls (metformin 500 mg/kg) as well as 9 diabetic groups that orally received 200, 400, and 800 mg/kg of extracts. The effects of the treatment with extracts for a 14-day period were investigated on weight, blood glucose, and lipid profile. By comparing the control groups with the groups of hydroalcoholic extracts of E. caeruleum showed that the most effective sample on weight gain and also on reducing blood glucose was the group receiving 800 mg/kg of the aerial branches extract (P < 0.01 and P < 0.001, respectively) after 14 days. As well, the most effective sample on lowering the blood lipid factors was the hydroalcoholic extract of the root of E. coareleum with a dose of 200 mg/kg, which showed a significant effect on lowering total cholesterol in diabetic rats compared to the diabetic controls (P < 0.05). Hydroalcoholic extract of leaves with 200 mg/kg also showed a better effect on lowering the LDL and VLDL levels compared to the diabetic control group (P < 0.001). The results of pancreatic histology in the samples showed that the extracts of the aerial branch and root (800 mg/kg) had significant effects on the regeneration of the islets of Langerhans compared to the diabetic control group. In conclusion, E. caeruleum could significantly improve glycemic and lipid profiles in diabetic rats.

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