scholarly journals POTENSI MAKROALGA PADINA AUSTRALIS (HAUCK 1887) DI BARAT SELATAN ACEH SEBAGAI AGEN INHIBITOR TIROSINASE

2018 ◽  
Vol 5 (1) ◽  
pp. 71
Author(s):  
Mohamad Gazali ◽  
Eri Safutra ◽  
Zulfadhli Zulfadhli
Keyword(s):  
Inhibitory Activity ◽  
Brown Algae ◽  
Methanol Extract ◽  
Bioactive Compound ◽  
Positive Control ◽  
Raw Material ◽  
Marine Macroalgae ◽  
Tyrosinase Inhibitor ◽  
Tyrosinase Inhibitory Activity ◽  
New Finding

Seaweeds are marine macro algae that can be found attach to the bottom shallow coastal waters. There are three major groups of seaweeds namely brown algae (Phaeophyta), red (Rhodophyta) and green (Chlorophyta). Padina australis is one of brown algae which mostly found in the West-South of Aceh coastal Area.  Seaweed contains bioactive compound which can serve as a defense from ultraviolet radiation that caused hyperpigmentation effect The purpose of this study is to analyse the tyrosinase inhibitory activity of P. australis extract from West-South Aceh. The results shown that the methanol extract of P. australis possess phytochemical properties such as flavonoids, tannin and saponin. tyrosinase inhibitory activity of P. australis  methanol extract  is the best extract which can be inhibit monophenolase with  IC50 : 293.520 µg/ml and IC50 = 13.571.067 µg/ml in diphenolase pathway with kojic acid as positive control. Moreover, chloroform and n-hexane extract have no activity of tyrosinase inhibitor. Therefore, new finding of tyrosinase inhibitor agent from marine macroalgae P. australis give the fruitfull information for cosmeceutical industry. P. australis parts could be complementary each other in providing the raw material of cosmetic product.

scholarly journals AKTIVITAS INHIBITOR TIROSINASE PADA EKSTRAK ALGA COKELAT SARGASSUM SP. AGARDH ASAL PESISIR LHOK BUBON, KABUPATEN ACEH BARAT

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Muhammad Gazali
Keyword(s):  
Inhibitory Activity ◽  
Brown Algae ◽  
Methanol Extract ◽  
Kojic Acid ◽  
Bioactive Compound ◽  
Positive Control ◽  
Marine Macroalgae ◽  
Tyrosinase Inhibitor ◽  
Tyrosinase Inhibitory Activity ◽  
New Finding

Seaweeds are marine macro algae that can be found attach to the bottom shallow coastal waters with subsrate as attached media. There are three major groups of seaweeds namely brown algae (Phaeophyta), red (Rhodophyta) and green (Chlorophyta). Sargassum sp is one of brown algae which mostly found in the Lhok Bubon Coastal West of Aceh. Recently, exploration of marine macroalgae as bioactive sources was investigated. Seaweed contains bioactive compound which can serve as a defense from ultraviolet radiation that caused hyperpigmentation effect. The aiming of this study is to analyse the tyrosinase inhibitory activity of Sargassum sp extract from Lhok Bubon Coastal Area, West of Aceh. The results shown that the methanol extract of Sargassum sp possess phytochemical properties such as fenol, alkaloid and triterpenoid. Tyrosinase inhibitory activity of Sargassum sp methanol extract  is the best extract which can be inhibit  monophenolase with  IC50 : 1111.49 µg/ml and IC50 = 1582.31 µg/ml in diphenolase pathway with kojic acid as positive control. Moreover, etyl asetate and n-hexane extract have no activity of tyrosinase inhibitor. Therefore, new finding of tyrosinase inhibitor agent from marine macroalgae Sargassum sp give the fruitfull information for cosmeceutical industry.Keywords : Lhok Bubon, Brown Algae, Sargassum sp, Tyrosinase Inhibitor

scholarly journals AKTIVITAS INHIBITOR TIROSINASERUMPUT LAUT Halimeda Spp DARI PESISIR ACEH BARAT

2018 ◽  
Vol 5 (2) ◽  
pp. 149 ◽  
Author(s):  
Mohamad Gazali
Keyword(s):  
Green Algae ◽  
Inhibitory Activity ◽  
Local Community ◽  
Methanol Extract ◽  
Bioactive Compound ◽  
Positive Control ◽  
Raw Material ◽  
Tyrosinase Inhibitory Activity ◽  
Traditional Drug ◽  
New Finding

Seaweeds have been utilized by local community as foodstuff, cosmeceutical, traditional drug and others. There are three major groups of seaweeds namely brown algae (Phaeophyta), red (Rhodophyta) and green (Chlorophyta). Halimeda sppis one of green algae which sufficiently dominant at the coastal of WestAceh. Seaweed contains bioactive compound which can serve as a defense from ultraviolet radiation that caused hyperpigmentation effect. The purpose of this study is to analyse the tyrosinase inhibitory activity of Halimeda sppextract from WestAceh. The results shown that the methanol extract of Halimeda spppossess phytochemical properties such as ekstrak Halimeda spp mengandung senyawa alkaloid, flavonoid, phenol, tannin and steroid. Tyrosinase inhibitory activity of Halimeda sppmethanol extract  is the best extract which can be inhibit monophenolase with  IC50 : 57300,660µg/ml and IC50 = 3981685,489µg/ml in diphenolase pathway with kojic acid as positive control. Moreover,etyl acetateand n-hexane extract have no activity of tyrosinaseinhibitor. Therefore, new finding of tyrosinase inhibitor agent from green algae Halimeda sppgive the fruitfull information forcosmeceutical industry. Halimeda sppparts could be complementaryeach other in providing the raw material of cosmetic product.

scholarly journals Evaluation of Antioxidant, Antimicrobial, Antityrosinase and Cytotoxic Potentials of Isatis cappadocica subsp. alyssifoli as a Potent Pharmaceutical Resource

2019 ◽  
pp. 1-12 ◽  
Author(s):  
Meltem Güner ◽  
Seyda Kanbolat ◽  
Nuriye Korkmaz ◽  
Rezzan Aliyazicioglu ◽  
Mahmoud Abudayyak ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Tyrosinase Inhibitor ◽  
Antioxidant Power ◽  
Tyrosinase Inhibitory Activity ◽  
Isatis Cappadocica

Isatis species, which are endemic across most of Turkey, exhibit antibacterial, anticancer and antiviral effects. The aim of this study was to determine of antioxidant, antimicrobial, tyrosinase inhibitor, cytotoxic activities, and phenolic profile of polar extracts of Isatis cappadocica Desv. We analyzed the antioxidant properties of extracts using total phenolic content (TPC), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), and 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging activity. The phenolic composition of methanolic extract of I. cappadocica was analyzed by reverse phase high performance liquid chromatography (RP-HPLC). Extracts’ antimicrobial properties were evaluated based on the agar well diffusion technique. Tyrosinase inhibitory activity was measured colorimetrically. Methanolic extract yielded better FRAP and CUPRAC results and aqueous extract yielded better DPPH activity. Benzoic acid, and sinapic acid were detected as major phenolic compounds. Methanolic extract was particularly effective against all the bacteria investigated, apart from Yersinia pseudotuberculosis. Methanol extract was exhibited tyrosinase inhibitory activity. The methanol extract has caused to death of cells by dosage in the high concentrations cytotoxic activity on the PC-3 and 3T3 cell lines. The results showed that I. cappadocica could be used as a natural source in the food, cosmetic, and drug industries due to their rich antioxidant, antimicrobial, cytotoxic and tyrosinase inhibitor activities.

Inhibition of Protein Tyrosine Phosphatase 1B by Lutein Derivatives isolated from Mexican Oregano (Lippia graveolens)

2018 ◽  
Vol 17 (3) ◽  
pp. 134-139
Author(s):  
R.M. Perez-Gutierrez
Keyword(s):  
Protein Tyrosine Phosphatase ◽  
Inhibitory Activity ◽  
Spectroscopic Data ◽  
Methanol Extract ◽  
Tyrosine Phosphatase ◽  
Positive Control ◽  
Protein Tyrosine Phosphatase 1B ◽  
Protein Tyrosine ◽  
Ic50 Value ◽  
Ic50 Values

Methanol extract from Lippia graveolens (Mexican oregano) was studied in order to identify inhibitory bioactives for protein tyrosine phosphatase 1B (PTP1B). Known flavone as lutein (1), and another flavone glycoside such as lutein-7-o-glucoside (2), 6-hydroxy-lutein-7-ohexoside (3) and lutein-7-o-ramnoide (4) were isolated from methanol extract of aerial parts of the Lippia graveolens. All isolates were identified based on extensive spectroscopic data analysis, including UV, IR, NMR, MS and compared with spectroscopic data previously reported. These flavones were evaluated for PTP1B inhibitory activity. Among them, compounds 1 and 3 displayed potential inhibitory activity against PTP1B with IC50 values of 7.01 ± 1.25 μg/ml and 18.4 μg/ml, respectively. In addition, compound 2 and 4 showed moderate inhibitory activity with an IC50 value of 23.8 ± 6.21 and 67.8 ± 5.80 μg/ml respectively. Among the four compounds, luteolin was found to be the most potent PTP1B inhibitor compared to the positive control ursolic acid, with an IC50 value of 8.12 ± 1.06 μg/ml. These results indicate that flavonoids constituents contained in Lippia graveolens can be considered as a natural source for the treatment of type 2 diabetes.

scholarly journals A New Phenolic Constituent From Carica papaya Flowers and Its Tyrosinase Inhibitory Activity

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1985098
Author(s):  
Giang Thi Kim Lien ◽  
Do Thi Thuy Van ◽  
Dao Hung Cuong ◽  
Pham Hai Yen ◽  
Bui Huu Tai ◽  
...  
Keyword(s):  
Spectral Data ◽  
Inhibitory Activity ◽  
Carica Papaya ◽  
Kojic Acid ◽  
Positive Control ◽  
Natural Source ◽  
Tyrosinase Inhibitory Activity ◽  
Esi Ms ◽  
Phenolic Constituent

A new phenolic (caricapapayol, 1) and 8 known compounds (2-9) were isolated from the flowers of Carica papaya. Their structures were determined by analysis of HR-ESI-MS, NMR spectral data, and comparison with the literature. Among known compounds, compound 2 has not been reported from natural source. Compounds 1, 2, and 4 exhibited tyrosinase inhibitory activity with IC50 values of 14.3 ± 2.7, 25.5 ± 1.9, and 19.8 ± 3.0 µM, respectively, in comparison with positive control kojic acid IC50 11.3 ± 1.6 µM.

Phytochemicals and Tyrosinase Inhibitory Activity from Piper caninum and Piper magnibaccum

2019 ◽  
Vol 25 (4) ◽  
pp. 358-363
Author(s):  
Nur Athirah Hashim ◽  
Farediah Ahmad ◽  
Wan Mohd Nuzul Hakimi Wan Salleh ◽  
Shamsul Khamis
Keyword(s):  
Inhibitory Activity ◽  
Kojic Acid ◽  
Biological Effects ◽  
Positive Control ◽  
Aromatic Plants ◽  
Tyrosinase Inhibitory Activity ◽  
Potential Source ◽  
Phytochemical Constituents ◽  
Reported Data ◽  
Ethyl Acetate Extracts

Background: Piper species are aromatic plants used as spices in the kitchen, but their secondary metabolites have also shown biological effects on human health. In traditional medicine, Piper species have been used worldwide to treat several diseases such as urological problems, skin, liver and stomach ailments, for wound healing, and as antipyretic and anti-inflammatory agents. In the present study, we attempted to isolate the phytochemicals from Piper caninum and Piper magnibaccum and evaluate their tyrosinase inhibitory activity. Methods: Phytochemical constituents of the extracts were investigated using various chromatographic and spectroscopic methods. The structures of the isolated phytochemicals were established by analysis of their spectroscopic data, as compared to that of reported data. Tyrosinase inhibitory activity was also tested on the extracts and selected compounds using mushroom tyrosinase as the enzyme. Results: Fractionation and purification of the extracts of Piper caninum and Piper magni­baccum afforded nine known compounds which were cepharanone A (1), cepharadione A (2), aristolactam AII (3), 5,7-dimethoxyflavone (4), 24-methylenecycloartan-3-one (5), β-sitosterol (6), piperumbellactam A (7), 24S-ethylcholesta-5,22,25-trien-3β-ol (8) and stigmast-3,6-dione (9). Ethyl acetate extracts from leaves of P. magnibaccum gave the highest inhibition value at 48.35%, while the tested compounds displayed weak tyrosinase activity compared to the positive control, kojic acid. Conclusion: These phytochemical results suggested that the extracts could assist as a potential source of bioactive compounds. Further research is needed in which the extract could possibly be exploited for pharmaceutical use.

scholarly journals Chemical constituents and theira-glucosidase inhibitory activity of the stem of Salacia chinensis L.

2021 ◽  
Vol 5 (3) ◽  
pp. first
Author(s):  
Huu Tho Le ◽  
Truc Thanh Thi Duong ◽  
Phu Hoang Dang ◽  
Truong Nhat Van Do ◽  
Hai Xuan Nguyen ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Back Pain ◽  
Inhibitory Activity ◽  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Positive Control ◽  
Ic50 Values ◽  
Treatment Of Diabetes ◽  
Chcl3 Fraction

Salacia chinensis L., known as “Chop mao” in Vietnam, is a climbing shrub that belongs to the Celastraceae family. The stem of S. chinensis L. is used as a traditional medicine for the treatment of diabetes, rheumatoid arthritis, back pain, … The dried powdered stem of S. chinensis L. was collected in Phu Yen province and was extracted with methanol to yield methanol extract. The methanol extract was suspended in H2O and partitioned successively with n-hexane, CHCl3, EtOAc to obtain n-hexane, CHCl3, EtOAc, and H2O fractions, respectively. The CHCl3 fraction was subjected to a series of chromatographic separation to afford four purified compounds including 3-oxolup-20(29)-en-30-al (1), betulin-3-caffeate (2), 2-(4-hydroxy-3,5-dimethoxylphenyl)ethanol (3), and acetosyringone (4). Their structures were elucidated on the basis of the spectroscopic analysis and comparison with literature data. The isolated compounds were tested for their α-glucosidase inhibitory activity. The result indicated that all compounds (1-4) possessed significant α-glucosidase at the testing concentration of 100 µM with the percent inhibition values of 9.5 ± 1.3, 70.89 ± 0.25, 44.2 ± 1.6, and 6.7 ± 1.7 %, respectively. In addition, betulin-3-caffeate (2) and 2-(4-hydroxy-3,5-dimethoxylphenyl)ethanol (3) show more potent α-glucosidase inhibitory activity, with IC50 values of 69.7 and 152.0 µM, respectively, than that of positive control acarbose (IC50 = 214.5 µM).

scholarly journals Camellia japonica Essential Oil Inhibits α-MSH-Induced Melanin Production and Tyrosinase Activity in B16F10 Melanoma Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Si Young Ha ◽  
Ji Young Jung ◽  
Jae-Kyung Yang
Keyword(s):  
Essential Oil ◽  
Inhibitory Activity ◽  
Treated Group ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Tyrosinase Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Camellia Japonica ◽  
Tyrosinase Inhibitory Activity ◽  
Natural Substances

Essential oils are aromatic oils extracted from the leaves, stems, peels, petals, and roots of aromatic plants grown in nature or grown in organic methods and have various medical effects as natural substances. The essential oil extracted from Camellia japonica seeds exhibits various functional properties; however, its tyrosinase inhibitory activity has not been investigated extensively. This study is performed to investigate the chemical composition and tyrosinase inhibitory activity of Camellia japonica seed essential oil (CJS-EO). Hexamethylcyclotrisiloxane (42.36%) and octamethylcyclotetrasiloxane (23.28%) are the two primary components of CJS-EO, as identified via gas chromatography-mass spectrometry. The inhibitory activities of CJS-EO and positive control arbutin are further evaluated against mushroom tyrosinase. The results show that CJS-EO and arbutin inhibit tyrosinase activity. Moreover, CJS-EO significantly inhibits melanogenesis in the α-melanocyte-stimulating hormone-treated group, and a significant amount of melanin is suppressed. To ascertain the cause of the CJS-EO tyrosinase inhibitory effect and melanin reduction effect, genetic and protein analyses are performed. Based on our results, we tentatively conclude that CJS-EO can inhibit melanocytes from harmful factors such as tyrosinase-related protein. These results demonstrate that CJS-EO possesses potent antityrosinase activity and may be a good skin-whitening agent.

scholarly journals Phenolic Constituents from the Heartwood of Artocapus Altilis and their Tyrosinase Inhibitory Activity

2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Mai Ha Khoa Nguyen ◽  
Hai Xuan Nguyen ◽  
Mai Thanh Thi Nguyen ◽  
Nhan Trung Nguyen
Keyword(s):  
Phenolic Compounds ◽  
Vanillic Acid ◽  
Inhibitory Activity ◽  
Kojic Acid ◽  
Positive Control ◽  
Coumaric Acid ◽  
Meoh Extract ◽  
Tyrosinase Inhibitory Activity ◽  
Phenolic Constituents ◽  
Inhibitory Activities

From the MeOH extract of the heartwood of Artocapus altilis, thirteen phenolic compounds have been isolated, namely curcumin (1), desmethoxycurcumin (2), retrodihydrochalcone (3), apigenin (4), tangeretin (5), nobiletin (6), O-methyldehydrodieugenol (7), dehydrodieugenol (8), β-hydroxypropiovanillone (9), p-coumaric acid (10), p-hydroxybenzaldehyde (11), vanillin (12), and vanillic acid (13). This is the first report on the presence of these compounds in the heartwood of A. altilis. Compounds 1, 2, and 10 showed more potent tyrosinase inhibitory activities, with IC50 values ranging from 2.3 to 42.0 μM, than the positive control kojic acid (IC50, 44.6 μM). The most active compound, p-coumaric acid (10) (IC50, 2.3 μM), was 22 times more active in tyrosinase inhibitory activity than kojic acid.

scholarly journals Tyrosinase Inhibitory Activity, 3D QSAR, and Molecular Docking Study of 2,5-Disubstituted-1,3,4-Oxadiazoles

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Ramesh L. Sawant ◽  
Prashant D. Lanke ◽  
Jyoti B. Wadekar
Keyword(s):  
Enzyme Inhibition ◽  
Inhibitory Activity ◽  
3D Qsar ◽  
Docking Study ◽  
Docking Studies ◽  
Maximum Activity ◽  
Tyrosinase Inhibitor ◽  
Molecular Docking Study ◽  
Tyrosinase Inhibitory Activity ◽  
Vlife Mds

In continuation with our research program, in search of potent enzyme tyrosinase inhibitor, a series of synthesized 2,5-disubstituted 1,3,4-oxadiazoles have been evaluated for enzyme tyrosinase inhibitory activity. Subsequently, 3D QSAR and docking studies were performed to find optimum structural requirements for potent enzyme tyrosinase inhibitor from this series. The synthesized 20 compounds of 2,5-disubstituted-1,3,4-oxadiazole series were screened for mushroom tyrosinase inhibitory activity at various concentrations by enzyme inhibition assay. The percentage enzyme inhibition was calculated by recording absorbance at 492 nm with microplate reader. 3D QSAR and docking studies were performed using VLife MDS 3.5 software. In the series 2,5-disubstituted-1,3,4-oxadiazoles enzyme tyrosinase inhibitory activity was found to be dose dependent with maximum activity for compounds4c,4h,4m, and4r. 3D QSAR and docking studies revealed that more electropositive and less bulky substituents if placed on 1,3,4-oxadiazole nucleus may result in better tyrosinase inhibitory activity in the series.

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