scholarly journals Antibacterial activity of Costus pulverulentus (Costaceae) C. Presl

2020 ◽  
pp. 1-13
Author(s):  
Luis Rodrigo Ramiro-Bautista ◽  
Alejandro Hernández-Morales ◽  
Candy Carranza-Álvarez ◽  
Juan José Maldonado-Miranda
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Ethanolic Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Bacillus Sp ◽  
Extract Fraction ◽  
Plant Stem ◽  
Agar Method ◽  
New Compounds

Antimicrobial resistance is a great concern in public health. Therefore, it is necessary to obtain new compounds to treat diseases caused by bacteria. Medicinal plants are an alternative to search natural compounds to improve human health, including antioxidants, anti-inflammatory, and antimicrobials compounds. Costus pulverulentus (Costaceae) C. Presl is a plant used traditionally in Huasteca Potosina to treat bacterial infections. However, the compounds involved in this activity remain poorly understood. To determine the antibacterial activity of C. pulverulentus, an ethanolic extract was obtained. Plant stem was macerated in ethanol and then was fractionated with hexane, chloroform, ethyl acetate, acetone, ethanol, and methanol. Ethanolic extract and derived fractions were tested against bacteria by the disk-diffusion agar method. The results showed that the ethanolic extract of C. pulverulentus exerted activity against Chromobacterium violaceum CV026 and Bacillus sp. at 10 and 30 μg/disk, whereas only the methanolic fraction showed similar activity to complete extract. Fraction 8 obtained from methanolic fraction showed inhibitory activity against Bacillus sp., S. aureus, and S. aureus Oxacillin resistant. Gas Chromatography-Mass Spectrometry characterization of active fraction 8 showed that it contains vanillic acid and pcoumaric acid suggesting that they are involved in the antibacterial activity of C. pulverulentus.

scholarly journals Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections

2021 ◽  
Vol 5 (1) ◽  
pp. 020-028
Author(s):  
Fernandes Laura Silva ◽  
da Costa Ygor Ferreira Garcia ◽  
de Bessa Martha Eunice ◽  
Ferreira Adriana Lucia Pires ◽  
do Amaral Corrêa José Otávio ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Metabolic Profile ◽  
Bacterial Infections ◽  
Ethanolic Extract ◽  
Multidrug Resistant ◽  
Gas Chromatography Mass Spectrometry ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
New Antibiotics

Morbidity and mortality of the infected patients by multidrug-resistant bacteria have increased, emphasizing the urgency of fight for the discovery of new innovative antibiotics. In this sense, natural products emerge as valuable sources of bioactive compounds. Among the biodiversity, Eryngium pristis Cham. & Schltdl. (Apiaceae Lindl.) is traditionally used to treat thrush and ulcers of throat and mouth, as diuretic and emmenagogue, but scarcely known as an antimicrobial agent. With this context in mind, the goals of this study were to investigate the metabolic profile and the antibacterial activity of ethanolic extract (EE-Ep) and hexane (HF-Ep), dichloromethane (DF-Ep), ethyl acetate (EAF-Ep) and butanol (BF-Ep) fractions from E. pristis leaves. Gas Chromatography-Mass Spectrometry (GC-MS) was performed to stablish the metabolic profile and revealed the presence of 12 and 14 compounds in EAF-Ep and HF-Ep, respectively. β-selinene, spathulenol, globulol, 2-methoxy-4-vinylphenol, α-amyrin, β-amyrin, and lupeol derivative were some of phytochemicals identified. The antibacterial activity was determined by Minimal Inhibitory Concentration (MIC) using the broth micro-dilution against eight ATCC® and five methicillin-resistant Staphylococcus aureus (MRSA) clinical strains. HF-Ep was the most effective (MIC ≤ 5,000 µg/µL), being active against the largest part of tested Gram-positive and Gram-negative bacterial strains, including MRSA, with exception of Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 9027) and (ATCC 27853). These results suggest that E. pristis is a natural source of bioactive compounds for the search of new antibiotics which can be an interesting therapeutic approach to recover patients mainly infected by MRSA strains.

scholarly journals Antibacterial Activity of Endostemon tereticaulis (Poir.) M. Ashby Essential Oil and Ethanolic Extract Against Resistant Pathogenic Bacteria

2020 ◽  
Vol 15 (9) ◽  
pp. 1934578X2095325
Author(s):  
Bala Namata Abba ◽  
Abderrahmane Romane ◽  
Amadou Tidjani Ilagouma
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Traditional Medicine ◽  
Pathogenic Bacteria ◽  
Ethanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Minimal Bactericidal Concentration

Endostemon tereticaulis (poir.) M.Ashby is a species of the Lamiaceae family present in Niger. This plant is used in traditional medicine due to its various biological potentialities. The present study investigated the chemical composition of the essential oil and the antibacterial activity of the essential oil and ethanolic extract of Endostemon tereticaulis against resistant pathogenic bacteria. Gas chromatography-mass spectrometry analysis of the essential oil led to the identification of 43 compounds representing 99.55% of the total essential oil. The major components were caryophyllene oxide (15.17%) followed by α-humulene (13.96%), α-copaene (11.75%), ( E)-β-caryophyllene (8.44%), and δ-cadinene (6.78%). The antibacterial activity was tested against multiresistant Acinetobacter baumannii P1483, Salmonella spp. H1548, extended-spectrum β-lactamase- Escherichia coli Bu8566, Enterobacter cloacae Bu147, Proteus mirabilis Bu190 , Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25922), Enterococcus faecium H3434, methicillin-resistant Staphylococcus aureus P1123, and Staphylococcus aureus (ATCC 25923). The antibacterial assays revealed that the essential oil was more active than the ethanolic extract against the studied bacteria with minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values ranging from 0.06 to 2 mg/mL. Also, the ethanolic extract was effective against the bacteria tested with MIC and MBC values ranging from 0.12 to 3 mg/mL. This study showed that Endostemon tereticaulis essential oil is rich in bioactive compounds. Ethanolic extract and essential oil exhibited potential antibacterial activity. These results provide a scientific basis for the use of this plant in traditional medicine. The current study described for the first time the antibacterial activity of Endostemon tereticaulis.

scholarly journals Green Synthesis and Characterization of Silver Nanoparticles using Crude Extract of Crotalaria burhia

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Khalil Ahmad ◽  
Raeesa Noor ◽  
Muhammad Younus ◽  
Akram Chohan ◽  
Ume Habiba ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Zeta Potential ◽  
Green Synthesis ◽  
Crude Extract ◽  
Bacterial Infections ◽  
Synthesis And Characterization ◽  
Phytochemical Analysis ◽  
E Coli

Background: Appearance of antibiotic resistance has raised the demand to find alternative therapies and modified drug delivery system of medicinal plants to treat bacterial infections. Objective: The aim of this study is the green synthesis and characterization of silver nanoparticles by using crude extract of Crotalaria burhia and to evaluate their antibacterial potential. Methods: The roots and stems of plant were used to prepare the crude extract. The phytochemical analysis of different compounds in extract was performed. 1mM AgNO3 and different concentrations of plant extract were used for the green synthesis of silver nanoparticles. The particles size and zeta potential were measured by zeta sizer while surface morphology of silver nanoparticles was observed with Scanning Electron Microscope (SEM). The antibacterial activity of silver nanoparticles was performed by 96 well microdilution plate method. Results: The particle size and zeta potential of optimized formulation was 92 nm and -24.8 mV. The SEM analysis showed that silver nanoparticles are irregular and spherical shape. The antibacterial activity showed that MIC value of silver nanoparticles was lower for E. coli than S. aureus. Conclusion: Silver nanoparticles possess potent bactericidal activity against E. coli and moderate activity against S. aureus. It had been concluded that these nanoparticles can be used against multi-drug resistant bacterial infections.

scholarly journals Chemical Characterization of Metabolites from the Husk of Theobroma cacao by GC-MS in Cuba

2018 ◽  
Vol 6 (2) ◽  
pp. 39
Author(s):  
Danae Perez ◽  
José González ◽  
Enrique Gómez ◽  
Julio Perez ◽  
José L. Rodríguez ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Phenolic Compounds ◽  
Theobroma Cacao ◽  
Chemical Characterization ◽  
Ethanolic Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Phytochemical Screening

n ethanolic extract at 90% made by Soxhlet was analyzed using a Shimadzu spectrometer coupled to gas chromatography (GCMS-QP2010 Ultra) after derivatization with BSTFA from the husk of Theobroma cacao. The drug powdered was evaluated through a phytochemical screening to get information about the chemical components present in the sample. Ethereal, ethanolic and aqueous extract were analyzed by the same GCMS technique. Gas Chromatography Mass Spectrometry (GC-MS) analysis revealed the presence of 74 metabolites from this husk. According to NIST21 and NIST107 Libraries Databases some of the phyto-compounds screened were Caffeine, Theobromine, Catechine, Uridine, Stigmasterol, Cholesterol and Androstane-11,17-dione,3-[(trimethylsilyl)oxy]-,17-[O-(phenyl methyl)oxime]. Phytochemical screening revealed the possible presence of fats, alkaloids, triterpenes and steroids, catechins, reductants sugars, saponins, phenolic compounds, aminoacids or amines, quinones, anthocyanidins, flavonoids and bitter or astringent principles.

scholarly journals Citriquinones A and B, New Benzoquinones from Penicillium Citrinum

2013 ◽  
Vol 8 (10) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
P. K. Vinitha Ranji ◽  
S. Chandrani Wijeyaratne ◽  
K. Hector Jayawardana ◽  
G. M. Kamal B. Gunaherath
Keyword(s):  
Antibacterial Activity ◽  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Human Cancer ◽  
Cancer Cell Line ◽  
Soil Fungus ◽  
Penicillium Citrinum ◽  
Bacillus Sp ◽  
Human Cancer Cell ◽  
New Compounds

Two new benzoquinones, citriquinone A (1) and B (2), were isolated from the methanol extract of a soil fungus, Penicillium citrinum. The structures of the new compounds were determined on the basis of detailed spectroscopic analysis. Citriquinone A (1) exhibited antibacterial activity against Bacillus sp. and cell migration inhibitory activity (CMIA) against human cancer cell line HEp 2.

Extraction and characterization of sea anemones compound and its Anti bacterial and hemolytic studies

2020 ◽  
Vol 10 (4) ◽  
pp. 93-97
Author(s):  
Anil Kumar A ◽  
Raja Sheker K ◽  
Naveen B ◽  
Abhilash G ◽  
Akila CR
Keyword(s):  
Antibacterial Activity ◽  
Focal Point ◽  
Haemolytic Activity ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Sea Anemones ◽  
Marine Plants ◽  
Heteractis Magnifica ◽  
Trididemnum Solidum

Seas assets that give us a variety of characteristic items to control bacterial, contagious and viral ailment and mostly utilized for malignancy chemotherapy practically from spineless creatures, for example, bryozoans, wipes, delicate corals, coelenterates, ocean fans, ocean bunnies, molluscs and echinoderms. In the previous 30 - 40 years, marine plants and creatures have been the focal point of overall endeavours to characterize the regular results of the marine condition. Numerous marine characteristic items have been effectively exceptional to the last phases of clinical preliminaries, including dolastatin-10, a group of peptides disengaged from Indian ocean rabbit, Dollabella auricularia. Ecteinascidin-743 from mangrove tunicate Ecteinascidia turbinata, Didemnins was isolated from Caribbean tunicate Trididemnum solidum and Conopeptides from cone snails (Conus sp.), and a developing number of up-and-comers have been chosen as promising leads for expanded pre-clinical appraisals. Sea anemones possess numerous tentacles containing stinging cells or cnidocytes. The stinging cells are equipped with small organelles known as nematocysts. The two species of sea anemones namely, Heteractis magnificaandStichodactyla haddoni, were collected from Mandapam coastal waters of Ramanathapuram district, Tamilnadu, India. The Nematocyst was collected and centrifuged, and the supernatant was lyophilized and stored for further analysis. The amount of protein from Heteractis Magnifica and Stichodactyla haddoni was estimated. The crude extract has shown haemolytic activity on chicken blood and goat blood. In the antibacterial activity of the sea anemone against six bacterial strains Staphylococcus aureus, Salmonella typhii, Salmonella paratyphii, Klebsiella pneumonia, Vibrio cholerae, Pseudomonas aeruginosa. Antibacterial activity of H. Magnifica and S.haddoni was measured as the radius of the zone of inhibition.

A comparative study of the antibacterial activity of Quercus robur (Ethanolic extract) and Zinc oxide nanoparticles on Salmonella (In vitro)

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Hams H. H. Alfattli ◽  
Ghufran Zuhair Jiber ◽  
Ghaidaa Gatea Abbass
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Quercus Robur ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Oxide Nanoparticles ◽  
Inhibition Zones

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.

Sign in / Sign up

Export Citation Format

Share Document