scholarly journals Deteksi Golongan Senyawa Ekstrak Kasar Metabolit Ekstrasel Mikroalga Laut Spirulina sp. Sebagai Agen Antioksidan

2021 ◽  
Vol 5 (1) ◽  
pp. 202
Author(s):  
Yulistia Anggraini ◽  
Diah Astika Winahyu
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ultraviolet Rays ◽  
Antioxidant Compounds ◽  
Extracellular Metabolite ◽  
Extracellular Metabolites ◽  
Component Identification ◽  
Dragendorff Reagent ◽  
Layer Chromatography

Microalgae excrete antioxidant compounds as a defense system to protect themselves from the danger of ultraviolet rays. These compounds also can be used as the organic materials of cosmetics or medicines. This study aimed to determine the antioxidant activity of marine microalgal extracellular metabolite extract of Spirulina sp.. Extracellular metabolites were extracted from the residual media filtrate from the harvesting. The qualitative antioxidant test’s results using the thin-layer chromatography technique and 2,2-diphenylpycrilhydrazil (DPPH) reagent showed antioxidant activity. Moreover, the component identification using ninhydrin and Dragendorff reagent in thin layer chromatography test showed alkaloid and peptide compounds. To support the results, the identification using infrared spectrum analysis showed the peaks at 1117 cm-1 (C-N and C-C stretching), 1458 cm-1 (C-H bending of methyl group), 1635 cm-1 (C=O stretching of amide group), and 3454 cm-1 (N-H stretching of amine and amide groups).

scholarly journals TLC analysis and antioxidant activity of garden egg leaves

2020 ◽  
Vol 12 (1) ◽  
pp. 443-448
Author(s):  
U.A. Umar ◽  
L.G. Hassan ◽  
K.L. Maradun
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Solanum Melongena ◽  
Solvent System ◽  
Antioxidant Compounds ◽  
Isolation And Characterization ◽  
Layer Chromatography

The Solanum melongena is used traditionally to treat different diseases such as cancer, atherosclerosis, and inflammation. This study is aimed at investigating the thin-layer chromatography analysis and antioxidant activity of n-hexane, acetone and methanol leaves extract of Solanum melongena. Sequential extraction with solvents of increasing polarity was carried out using the n-hexane, ethyl acetate and methanol. The thin-layer chromatography of the extracts carried out with different solvent system. Antioxidant activity was evaluated quantitatively using DPPH (2, 2-diphenyl-2-picrylhydrazyl) for its free radical scavenging ability. The results of thin-layer chromatography revealed some spots with Rf values in the respective extracts, n-hexane (0.31, 0.42, 0.59, 0.65, 0.72, 0.85, 0.92), acetone (0.97, 0.92, 0.88 and 0.59) and methanol (0.72, 0.83, 0.86, 0.94 and 0.96). The extracts exhibit strong antioxidant activities as radical scavengers, indicating that they have strong proton donating abilities. The results from this research show credence to the traditional application of the plant. Further research is recommended on the isolation and characterization of the antioxidant compounds from the plant. Key words: DPPH (2, 2-diphenyl-2-picrylhydrazyl), Thin layer chromatography, Reactive Oxygen Species (ROS)  

scholarly journals Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Margarita Cid-Hernández ◽  
Fernando Antonio López Dellamary-Toral ◽  
Luis Javier González-Ortiz ◽  
María Judith Sánchez-Peña ◽  
Fermín Paul Pacheco-Moisés
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Spirulina Platensis ◽  
Binary Systems ◽  
Experimental System ◽  
Biologically Active ◽  
Antioxidant Compounds ◽  
Two Dimensional ◽  
Layer Chromatography

Spirulina platensis contains several biologically active compounds, some of them with antioxidant activity. Nevertheless, not all of these compounds have been identified to date. As a first step to achieving such identification, a methodology to perform two-dimensional thin layer chromatography bioautographies on silica gel thin layer chromatography plates was proposed. Starting with a reference binary system, 5 other binary systems were tested, in which the relative polarity was systematically increased. To further improve the separation behavior, a phase modifier (NH4OH) was used. The best separation results were obtained with the isopropyl alcohol/ethyl acetate/NH4OH ternary system. This experimental system allowed four well-resolved spots showing antioxidant activity as well as two additional areas with mixtures containing antioxidant compounds. Although the proposed methodology was designed with a specific application, it would be predictable that its field of use could be considerably greater, making the convenient modifications on the solvent polarity and “masking level” produced by the ammonium derivatives.

scholarly journals Application of the thin-layer chromatography method for analysis of plant antioxidant activity

Food systems ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 26-30
Author(s):  
N. V. Kupaeva ◽  
E. R. Vasilevskaya ◽  
L. V. Fedulova ◽  
E. A. Kotenkova
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Chromatography Method ◽  
Physicochemical Methods ◽  
Ethanol Extracts ◽  
Layer Chromatography ◽  
Active Substances

Plants are a rich source of effective non-toxic biologically active substances. Various physicochemical methods of analysis are used for evaluation of plant antioxidant activity. Composition of ethanol extracts of red, yellow and white onion husks, dried rosemary, basil, and chaga were studied by high performance thin layer chromatography (HPTLC) method. The antioxidant activity of the obtained fractions on a chromatographic plate was assessed by subsequent DPPH screening. The extracts red and yellow onion husk and rosemary demonstrated the highest antioxidant activity, variability of the qualitative composition and similarity of antioxidant profiles, while extract of white onion husks did not contain any antioxidant classes. Intensive spots with Rf of 0.13-0.97 were observed along the whole chromatogram track corresponding to red onion husks. It was also found that all tested extract, excepting white onion husk and chaga, contained spots with varying degrees of intensity in the Rf range of 0.96-0.98, which corresponded quercetin Rf value.

scholarly journals Synthesis, Characterization and Antioxidant Activity of 2-Aryl Benzimidazole Derivatives

2020 ◽  
Vol 8 (2) ◽  
pp. 35-44
Author(s):  
Vineet Kumar Singh ◽  
Amrita Parle
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Radical Scavenging ◽  
Aromatic Aldehydes ◽  
Benzimidazole Derivatives ◽  
Benzoic Acids ◽  
Antioxidant Assay ◽  
Layer Chromatography

Objective: To synthesize benzimidazole derivatives, characterize them by 1HNMR and ATIR techniques and evaluate their antioxidant activity. Methods: In the present study 19 benzimidazole derivatives were synthesized by reacting O-phenylenediamine as the primary reactant with different aromatic aldehydes and benzoic acids. Reactions were monitored using thin layer chromatography technique, and the newly synthesized derivatives were characterized by ATIR and 1HNMR techniques. The antioxidant assay was performed using ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] method and DPPH [2,2-diphenyl-1-picrylhydrazyl] method. Results: Compounds BNZ-1, BNZ-2, BNZ-3, BNZ-9, and BNZ-10 showed comparable antioxidant activity to ascorbic acid at higher dose. Conclusion: The synthesized benzimidazole derivatives have significant radical scavenging potential.    

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF BIXIN PIGMENT FROM ANNATTO (Bixa orellana L.) SEEDS

2010 ◽  
Vol 7 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Pipin T. Kurniawati ◽  
H. Soetjipto ◽  
Leenawati Limantara
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bixa Orellana ◽  
Isolation And Identification ◽  
Double Beam ◽  
Layer Chromatography

Research on Bixa orellana L. have been done to isolate, identify and determine bixin percentage, the antioxidant and antibacterial activities of bixin from B. orellana seed.  Isolation and identification of bixin was done by thin layer chromatography (TLC), column chromatography, chemical test of bixin and UV-Vis double beam spectroscopy. Percentage of bixin was calculated by JECFA method, the antioxidant activity was determined by DPPH (1-1 diphynilpicrylhidrazil) method while antibacterial activity was analyzed by the use of agar diffusion method. Thin layer chromatography (TLC) for the crude extract contained 5 spot, where spot 5th was bixin. Bixa orellana has 75±3% of bixin. Antioxidant activity of bixin had IC50 548.5±20.0 ppm. Whereas the antibacterial activity of bixin against the Escherichia coli and Staphylococus aureus could be classified as weak inhibition category at 500-750 μg and medium inhibition category at 1500 μg.   Keywords: Bixa orellana L., bixin, antioxidant, antibacteria

scholarly journals Comparison of Antioxidant Activity and Main Active Compounds Among Different Parts of Alpinia officinarum Hance Using High-Performance Thin Layer Chromatography-Bioautography

2019 ◽  
Vol 102 (3) ◽  
pp. 726-733 ◽  
Author(s):  
Wan-Xin Zhang ◽  
In-Cheng Chao ◽  
De-jun Hu ◽  
Farid Shakerian ◽  
Liya Ge ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Quality Control ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Aerial Parts ◽  
Alpinia Officinarum ◽  
Layer Chromatography ◽  
Different Parts ◽  
Tof Ms

Abstract Background: Alpinia officinarum Hance (ginger family) is an important Chinese medicine, especially in Southern China. Objective: A simple and effective high-performance thin-layer chromatography coupled with 2, 2-diphenyl-1-picrylhydrazyl bioautography (HPTLC-DPPH) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) method was developed for the bioactivity-based quality control of A. officinarum. Methods: The HPTLC-DPPH and ESI-Q-TOF-MS/MS were applied for the analysis of different parts of A. officinarum after using methanol extraction for 23 batches of taproot, four batches of aerial, and three batches of fibril parts. Results: The systematic evaluation showed that similar components in taproot and aerial parts make the major antioxidant activity. However, based on our evaluation, the antioxidant ability of the aerial parts is lower than the taproot parts. There is also a significant difference (P < 0.05) between taproot and fibril parts of the root. The chemical structures of compounds with the antioxidant capacity were tentatively identified as 5R-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone (band 1), kaempferide (band 2), and galangin (band 3) based on ESI-Q-TOF-MS/MS analytical results and further confirmed by standards. Conclusions: This identification indicated that two flavonoid compounds and one diarylheptanoid compound possessed high potentials to be used as the antioxidant biomarkers for the quality control of A. officinarum. Highlights: The comparison of different parts could be considered as guidelines for the usage of A. officinarum.

scholarly journals SCREENING OF THE ANTIOXIDANT ACTIVITY OF CRUDE EXTRACTS IN 86 ALGAE SPECIES FROM EL JADIDA COAST (MOROCCO)

2019 ◽  
pp. 54-61 ◽  
Author(s):  
Fatima Chibi ◽  
Halima Rchid ◽  
Wafa Arsalane ◽  
Rachid Nmila
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Algal Species ◽  
Marine Macroalgae ◽  
Rapid Screening ◽  
Fucus Spiralis ◽  
El Jadida ◽  
Promising Source ◽  
Layer Chromatography

Objective: This work aimed to screen the antioxidant activity of marine macroalgae from the Moroccan Atlantic coast (region of El Jadida). Methods: Evaluation of the antioxidant activity of different collected species, lyophilized and extracted with a solvent mixture chloroform/methanol (2/1; v/v) was conducted according to two techniques, first by thin layer chromatography (tlc) then by spectrophotometry, using a free radical 2,2-diphenyl-1-picrylhydrazyl (dpph). The sampling on a distance of 110 km allowed to harvest 86 algal species (16 brown algae, 47 red algae, 14 green algae and 9 algae being identified). Results: The analysis by thin layer chromatography reveals an antioxidant activity in nearly half of harvested algal species (52.32 %). This activity varies depending on the concentration of the extract and in function of incubation time in the presence of dpph. The monitoring of the kinetics of degradation of dpph by spectrophotometer in the presence of extracts which were active by tlc allowed to confirm the results and select the most active algal species based on the percentage of remaining dpph in the medium after 120 min of reaction: Fucus spiralis (17.02 %), Cyctoseira ericoides (12.16 %) (Phaeophyceae), and Gracilaria multipartita (36%), Halopitys incurvus (5%) (Rhodophyceae). Conclusion: The results show that the methodology adopted in this work is reliable and can be used for rapid screening of antioxidant property in plants and the species: Fucus spiralis, Cyctoseira ericoides, Gracilaria multipartita, and Halopitys incurvus can be a promising source of natural compounds endowed with high antioxidant potential.

scholarly journals MARKET SAMPLE SURVEY OF CROCUS SATIVUS LINN. TO ASSESS THE GENUINITY FOR USING ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC CONTENT AND HIGH-PRESSURE THIN LAYER CHROMATOGRAPHY USING DETECTION OF FLAVONOIDS

2019 ◽  
pp. 30-33
Author(s):  
K. R. ATHIRA ◽  
T. V. BINU
Keyword(s):  
Antioxidant Activity ◽  
High Pressure ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
International Markets ◽  
Crocus Sativus ◽  
Total Phenolic ◽  
Layer Chromatography

Objective: Herbalism is a traditional medicine or folk medicine practice based on the use of plants and plant extracts. Many of the drugs used in conventional medicine are dried from herbs. Despite the fluctuation in prices in international markets, saffron was still remained the most expensive spice. The main aim of this study is to examine the antioxidant activity, total phenolic content, high-pressure thin layer chromatography using flavanoid analysis and adulteration detection of saffron. Crocus sativus. Linn is a perennial stemless herb of the Iridaceae family. Saffron stigmas of sample1, sample2, sample3and sample4 are collected from different rates of the market sample from Thrissur district, sample5 collected from the Oushadhi premises, and it is collected from Himachal Pradesh. Methods: In this study detecting the antioxidant activity, total phenolic content, high-pressure thin layer chromatography using flavanoid analysis of different samples of saffron stigmas. The extracts were prepared by using ethanol as a solvent. Results: Safranal is present only in s5 sample. It is the main essential volatile oil responsible for the saffron characteristic such as odour. Phenolic content is varied in different market samples. The amount of phenolic compounds in the saffron extract was determined using the Folin-ciocalteau reagent. Total phenolic content is the help to detect the pure and fake saffron. The phenolic content is higher in S5. Sample S5 showed 0.737 mg/ml phenolic content. Lowest level of phenolic content in sample S3. Sample S3 showed 0.0887 mg/ml phenolic content. Sample S4 showed 0.564 mg/ml total phenolic content. Sample S1 showed 0.416 mg/ml total phenolic content and sample S2 showed 0.267 mg/ml phenolic content. Antioxidant activity is higher in sample s5. and it is different in different market samples. Sample 5 stigma posses higher antioxidant activity. Sample S5 showing 14.88% antioxidant activity in 100 mg/ml concentration, 7.26% in 80 mg/ml concentration, 2.23% in 60 mg/ml concentration, 2.21% in 40 mg/ml and 1.01% in 20 mg/ml concentration. Sample S3 showed the lower antioxidant activity in 0.1% in 60 mg/ml concentration and 0.1% in 80 mg/ml. Ascorbic acid standard showing 14.89% in 100 mg/ml concentration, 7.26% in 80 mg/ml concentration, 4.56% in 60 mg/ml concentration, and 3.1% in 40 mg/ml concentration, and 1% in 20 mg/ml concentration. Flavonoid content is different in different samples. It is present highly present in sample s1 and s5. sample s3 do not contain the Flavanoid. The quality of the samples depend on the price values. Conclusion: The authenticity of saffron is an extremely important matter for the industry and for the consumers in view of security and protection,quality assurance, active properties and last but not least, economic impact. Despite the fluctuation in prices in international markets, saffron was and still remains the most expensive spice. The genuine saffron samples possess higher price value. The fake saffron available in the market with lower price value. The quality of the saffron depends upon the price values. These observations would be of immense value in the botanical identification and standardization of the drug in crude form and would help to distinguish the drug from its other spices.

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