scholarly journals KEBERADAAN MIKROBA PADA BAKSO IKAN ASAP CAIR, YANG DIKEMAS DALAM RETORTABLE POUCH, DIPASTEURISASI DAN DISIMPAN PADA TEMPERATUR RUANG

2016 ◽  
Vol 4 (2) ◽  
pp. 85
Author(s):  
Margani Luyuani Rohana ◽  
Siegfried Berhimpon ◽  
Joyce CV Palenewen
Keyword(s):  
Shelf Life ◽  
Water Content ◽  
Experimental Method ◽  
Room Temperature ◽  
Plate Count ◽  
E Coli ◽  
Existing Problems ◽  
Liquid Smoke ◽  
Total Plate Count ◽  
And Storage

A research has been done to assess the presence of microbes and the shelf life of fish balls dipped in liquid smoke, packed in retortable pouch, pasteurized, and stored at room temperature. The method used in this research is an experimental method that revealed the facts based on existing problems through hypothesis testing. Parameters assessed are Total Plate Count (TPC), total Salmonella, total coliforms and E. coli, total vibrio, water content, and pH. Fish balls were pasteurized in 85ºC and stored in room temperature for 0, 3, 6, and 9 days, and were pasteurized at 100°C and storage in room temperature for 0, 9, 18, and 27 days. The results shown that all pathogen were negative, but based on Indonesian standard (SNI 01-7266-1-2006) especially TPC value, fish balls were packaged in retortable pouch and without pasteurized is no longer acceptable for consumption after 3 days of storage, and fish balls were packaged in retortable pouch and pasteurized at 85ºC no longer acceptable for consumed after 6 days of storage. While the fish balls are packaged in retortable pouch and pasteurized at 100°C is no longer acceptable for consumed after 18 days of storage.Keyword: Retortable Pouch, Pasteurization, Pathogens.  Penelitian ini bertujuan untuk mengetahui keberadaan mikroba dan daya awet bakso ikan yang direndam dalam asap cair dan dikemas dalam Retortable pouch, dipasteurisasi, dan disimpan pada temperatur ruang. Metode yang digunakan dalam penelitian ini adalah metode eksperimen untuk menguji hipotesa. Parameter yang diamati yaitu Angka Lempeng Total (ALT), Salmonella, total koliform dan E.coli, total vibrio, analisa kadar air, dan nilai pH. Bakso ikan asap cair yang dipasteurisasi pada 85ºC, disimpan pada temperatur ruang selama 0, 3, 6, dan 9 hari, dan yang dipasteurisasi pada 100ºC, disimpan pada temperatur ruang selama 0,9,18, dan 27 hari. Hasil penelitian menunjukkan bahwa bakso ikan yang dikemas dalam Retortable pouch, tanpa dipasteurisasi sudah tidak layak lagi untuk dikonsumsi setelah penyimpanan 3 hari, dan bakso ikan yang dikemas dalam Retortable pouch dan dipasteurisasi pada 85ºC sudah tidak layak lagi untuk dikonsumsi setelah penyimpanan 6 hari. Bakso ikan yang dikemas dalam Retortable pouch, dan dipasteurisasi pada 100ºC nanti tidak layak lagi dikonsumsi setelah penyimpanan 18 hari.Kata Kunci: Kemasan Retortable Pouch, Pasteurisasi, Patogen.

scholarly journals Application of Liquid Smoke from Coconut Shell in Tandipang (Dussumeiria Acutta) Smoked Fish To Extend Shelf Life

2020 ◽  
Vol 12 (2) ◽  
pp. 315
Author(s):  
Dyah Ayu Rakhmayeni ◽  
Tatty Yuniarti ◽  
Sukarno Sukarno
Keyword(s):  
Activation Energy ◽  
Shelf Life ◽  
Room Temperature ◽  
Plate Count ◽  
Fish Products ◽  
Smoked Fish ◽  
Liquid Smoke ◽  
Quality Degradation ◽  
Total Plate Count ◽  
Different Temperatures

Highlightaw on smoked tandipang fish analyzed.Peroxide value on smoked tandipang fish analyzed.TPC on smoked tandipang fish analyzed.The shelf life of liquid smoked tandipangfish stored at 27o C was obtained the value of t = 23.2 in units of weeks or 5.8 months of storage.AbstractFumigation using liquid smoke produces smoked fish products with a smoky flavor character and can increase shelf life. The research objective was to determine the shelf life of the tandipang fish which was processed using liquid smoking. The test was carried out by storing smoked fish in an incubator at different temperatures 30 °C, 40 °C, and 50 °C with three replications for 5 weeks (35 days). The samples prepared at each temperature were kept in an incubator for five weeks. The parameters tested for estimating shelf life are using the test data aW (activity water), peroxide number, and TPC (total plate count). The three parameters are selected by one parameter to calculate the shelf life of liquid smoked tandipang fish which is considered to greatly affect the quality degradation during storage. The aw parameter is used to determine the shelf life because it has a high R2 value with an activation energy of 86972.75 J / mol.K. Estimation of shelf life of liquid cured fish at room temperature using aw parameter is 23.2 weeks or 5.8 months.

scholarly journals Methyl Red Indicator on Smart Packaging as a Freshness Sensor for Tilapia Fillets

2019 ◽  
pp. 1-9
Author(s):  
Kinanthi Ayu Sariningsih ◽  
Iis Rostini ◽  
Kiki Haetami
Keyword(s):  
Shelf Life ◽  
Room Temperature ◽  
Plate Count ◽  
Methyl Red ◽  
Total Volatile ◽  
Film Sensor ◽  
Smart Packaging ◽  
Color Changes ◽  
Total Plate Count ◽  
Tilapia Fillets

This research aims to analyze the response of methyl red indicator film sensor label color changes on smart packaging in detecting the freshness of tilapia fillets over a shelf life of 10 hours at room temperature (± 25ºC). This research uses a comparative descriptive method. The parameters observed were pH values, total plate count (TPC) and total volatile base nitrogen (TVBN). The results of this research indicate that the response of the film label consists of several concentrations of methyl red namely control (0%); 0.05%; 0.5% and 1%. The best results from film sensor label response is 0.05% Methyl red concentration in detecting the freshness of tilapia fillets and showed the most rapid response to changing the color of the label at the observation of the 7th hours during storage of 10 hours with 8 times the observation time. The freshness of tilapia fillets has decreased quality with changes in pH that tend to fluctuate ie the pH has decreased and then rose again as the color changes are indicated by the film label from yellow to reddish-orange. Total volatile base nitrogen (TVBN) and total plate count (TPC) during the observation showed a trend towards the same graph results, namely, the longer shelf life will increase the decrease in freshness quality of tilapia fillets with marked decay. Therefore, the freshness level of tilapia fillets can be determined by the smart packaging sensor methyl red indicator label at room temperature storage (± 25ºC).

scholarly journals Perubahan Fisiko-Kimiawi dan Mikrobiologis Minuman Tradisional Bali (Loloh) selama Penyimpanan

2019 ◽  
Vol 39 (1) ◽  
pp. 70
Author(s):  
I Desak Putu Kartika Pratiwi ◽  
I Ketut Suter ◽  
Putu Ari Sandhi Widpradnyadewi ◽  
Anak Agung Istri Sri Wiadnyani
Keyword(s):  
Vitamin C ◽  
Shelf Life ◽  
Room Temperature ◽  
Titratable Acidity ◽  
Plate Count ◽  
Total Plate Count ◽  
Microbiological Characteristics ◽  
Physico Chemical ◽  
Soluble Solid ◽  
Standard Limit

Loloh is a Balinese traditional beverage made from one or a combination of several herbal extracts. The most popular variants of loloh in Bali are loloh tibah and loloh cem-cem, which are continuously produced every day. During distribution, loloh is stored at room temperature. The study was aimed to study the physico-chemical and microbiological characteristics of loloh cem-cem and loloh tibah during storage at room temperature.  This study used a purposive random sampling. The samples consisted of 14 loloh sellers in Badung-Denpasar. Type parameters that were observed during a 24 hour-storage at room temperature included pH, total soluble solid, titratable acidity, ascorbic acid, total plate count, total yeast and mold count. During room temperature storage, the level of total plate count, total mold/yeast of loloh cem-cem and loloh tibah changed significantly. The shelf life of loloh cem-cem was only 12 hours at room temperature (28-30 oC) with the characteristics of pH 2.95, TSS 5.67 °Brix, vitamin C 19.35 mg/100 g, titratable acidity 0.24%, log total plate count 6.48 Cfu/mL, log total mold/yeast 5.01 Cfu/mL. The shelf life of Loloh tibah was only 12 hours at room temperature (28-30 ºC) with the characteristics of pH 3.34, TSS 4.21 °Brix, Vitamin C 10.20 mg/100 g, titratable acidity 0.13%, log total plate count 6.68 Cfu/mL, log total mold/yeast 5.86 Cfu/mL. Beyond the 12 hour-storage, both of the lolohs had higher total colony counts than the upper standard limit.

scholarly journals Pengaruh Konsentrasi Asap Cair Terhadap Mutu, Total Bakteri, dan Waktu Simpan Ikan Cakalang (Katsuwonus pelamis) Asap

KOVALEN ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 59-64
Author(s):  
Angria Deswika Malambu ◽  
Syaiful Bahri ◽  
Hardi Ys. ◽  
Prismawiryanti ◽  
Abd. Rahman Razak
Keyword(s):  
Protein Content ◽  
Storage Time ◽  
Plate Count ◽  
Katsuwonus Pelamis ◽  
Smoked Fish ◽  
Liquid Smoke ◽  
Total Plate Count ◽  
And Storage ◽  
Total Bacteria

Liquid smoke contains acid, carbonyl, and phenolic compounds, so it is very appropriate to be used as a preservative. Traditional smoking can be replaced with liquid smoke because it is safer and more practical. The study of the use of coconut shell liquid smoke has been used in skipjack fish to determine the effect of liquid smoke concentration and storage time on dissolved protein content and total bacteria in smoked fish. The quality of smoked fish was determined by two treatments, namely the variation in the concentration of liquid smoke (2, 3, 4, and 5%) and storage time (2, 4, 6, 8, and 10 days). The results showed that the best concentration of liquid smoke to produce smoked skipjack fish is 3% with a water content of 62.41% and dissolved protein content of 17.58%. The maximum storage time of smoked skipjack tuna is 6 days with a total plate count (TPC) value or total bacteria 1.8 x 105.

scholarly journals PENERAPAN PENGASAPAN CAIR PADA PENGOLAHAN ABON ROA (Hemirhamphus sp.) DAN PAMPIS CAKALANG (Katsuwonus pelamis L) DAN MUTU MIKROBIOLOGIS PRODUK YANG DIKEMAS MODIFIED ATMOSPHERIC PACKAGING (MAP)

2017 ◽  
Vol 5 (1) ◽  
pp. 8
Author(s):  
Chintia S.F. Bawole ◽  
Feny Mentang ◽  
Henny Adeleida Dien
Keyword(s):  
Room Temperature ◽  
Microbiological Quality ◽  
Plate Count ◽  
Katsuwonus Pelamis ◽  
E Coli ◽  
North Sulawesi ◽  
Modified Atmospheric Packaging ◽  
Total Plate Count

Smoked Garp fish and smoke Skipjack a typical processed product in North Sulawesi. Roa smoke and smoke Skipjack can be further processed into abon Roa and Skipjack pampis, abon fish are foods processed fish flavored, processed by boiling and frying obtain dry product, while pampis fish is processed product of smoke Skipjack flavored and obtain the product half wet. The purpose of this study is to see and observe the presence of bacteria on the product abon Roa and Skipjack pampis are packed Modified Atmospheric Packaging (MAP) and stored at room temperature for 30 days. Total Plate Count (TPC),total of Salmonella sp., total E. coli and total Vibrio sp. observed every 10 days, at 0, 10, 20, and 30 days of storage. Based on observations Total Plate Count, abon Roa and Skipjack pampis can last for 30 days with the highest TPC value of 6.3x104 CFU/g and still be consumed. While Total Salmonella sp, total E. coli and total Vibrio sp. indicates a negative value to the day to 30 has a negative value. Keyword: Microbiological quality, abon Roa, Skicjack pampis, MAP packaging.   Roa asap dan Cakalang fufu merupakan produk olahan ikan asap khas Sulawesi Utara. Roa asap dan Cakalang fufu dapat diolah lebih lanjut menjadi abon roa dan pampis cakalang, abon ikan merupakan jenis makanan olahan ikan yang diberi bumbu, diolah dengan cara perebusan dan penggorengan yang memperoleh produk kering, sedangkan pampis ikan adalah produk olahan dari cakalang fufu yang diberi bumbu dan memperoleh produk setengah basah. Tujuan penelitian ini yaitu untuk melihat dan mengamati keberadaan bakteri pada produk abon Roa dan pampis Cakalang yang dikemas Modified Atmospheric Packaging (MAP) dan disimpan pada suhu ruang selama 30 hari. Angka Lempeng Total (ALT), total Salmonella sp, total E. coli dan total Vibrio sp diamati setiap 10 hari yaitu pada 0, 10, 20, dan 30 hari penyimpanan. Berdasarkan hasil pengamatan Angka Lempeng Total, abon Roa dan pampis Cakalang dapat bertahan selama 30 hari dengan nilai ALT tertinggi 6,3x104 CFU/g dan masih bisa dikonsumsi. Sedangkan Total Salmonella sp, total E. coli dan total Vibrio sp menunjukkan nilai negatif sampai pada hari ke 30 memiliki nilai negatif. Kata Kunci: Mutu mikrobiologis, abon Roa, pampis Cakalang, kemasan MAP.

scholarly journals CHARACTERISTICS OF DURIAN SEED BROWNIES WHICH ENRICHED WITH COCONUT FLOUR

2019 ◽  
Vol 2 (01) ◽  
pp. 06-09
Author(s):  
Ade Heri Mulyati ◽  
Diana Widyiastuti ◽  
Ikhwanul Muslimin
Keyword(s):  
Bacillus Cereus ◽  
Shelf Life ◽  
Water Content ◽  
Dietary Fiber ◽  
Room Temperature ◽  
Future Studies ◽  
E Coli ◽  
Seed Flour ◽  
Engine Coolant ◽  
Durian Seed

Durian seed flour is a product selected and used as a fixed variable in future studies. The results of chemical analysis and micro Brownies F2 have a water content of 26.47%, ash 1.63%, 16.56% fat, 6.91% protein, carbohydrates 48.43%,% dietary fiber, 4 ALT tests, 3 x 102 cabbage / g, e- coli <3, mold <10 Bacillus Cereus <100. In a subsequent study, Brownies steamed (F8) with a ratio of 75% wheat flour and seed flour 25% enriched 20% Durian pulp has the water content of 26.35%, ash 1.65%, 19.58% fat, 7.20% protein, carbohydrates 48.53 %, 12.53% dietary fiber, 2.7 x 102 ALT test cabbage / g, e-coli <3, mold <10 Bacillus Cereus <100. The shelf life of the conventional product at room temperature (28-30oC), Brownies control F1 has the shelf life of 3 days while the Brownies F8 is 6 days, the engine coolant (2- 8oc) Brownies control F1 has the shelf life of 10 days while Brownies F8 14 days.

scholarly journals MUTU MIKROBIOLOGIS BAKSO IKAN YANG DIRENDAM ASAP CAIR, DIKEMAS VAKUM, DIPASTEURISASI DAN DISIMPAN PADA SUHU DINGIN

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Oktavianus Alexander Poluakan ◽  
Henny Adeleida Dien ◽  
Frans Gruber Ijong
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Plate Count ◽  
High Water Content ◽  
Cold Temperatures ◽  
E Coli ◽  
Liquid Smoke ◽  
Total Plate Count ◽  
Spoilage Bacteria

Fish meatball is a processed fish product and quite popular for the public. The weakness of fish meatball is quickly decayed which is caused by the growth of spoilage bacteria and pathogens during the storage. Liquid smoke has a function as a barrier to the development of bacteria. Pasteurization, vacuum packaging and storage of cold temperatures could inhibit the growth of bacterium. The purpose of these study is to assessment the Total Plate Count, Total Coliform and Escherichia coli, and Total Salmonella for the microbiological quality of fish meatball soaked in liquid-smoked, vacuum packed, pasteurized and stored at cold temperatures. Fish meatball soaked in liquid smoke 0.8% for 30 minutes and pasteurized at temperature of ±88ºC for 30, 60 and 90 minutes, then vacuum packed and stored at cold temperatures. Based on the results of observations, Total Plate Count from fish meatball can last up for 10 days with the highest TPC value of 6.3 x 104 CFU / g according to ISO 7266: 2014. Total Salmonella until a 20th day had a negative value. Also, Total Coliform and E. coli growth does not found in the cold storage for 30 days. Value fish balls high water content and pH are good for bacterial growth. Immersion in liquid-smoked indicates that liquid-smoked is a good antibacterial preservative for use as an additive. Keyword: Fish Meatball, Liquid-Smoked, Pasteurization, Bacteria.   Bakso ikan merupakan produk olahan ikan yang cukup digemari masyarakat. Kelemahan dari bakso ikan yaitu cepat mengalami pembusukan yang diakibatkan oleh pertumbuhan bakteri pembusuk dan patogen selama masa simpan. Asap cair memiliki fungsi sebagai penghambat perkembangan bakteri. Pasteurisasi, Pengemasan secara vakum dan penyimpanan suhu dingin dilakukan untuk menghambat pertumbuhan bakteri. Penelitian ini bertujuan untuk menghitung Angka Lempeng Total, Total Salmonella dan Total Koliform dan Escherichia coli terhadap mutu mikrobiologis bakso ikan yang direndam asap cair, dikemas vakum, dipasteurisasi dan disimpan pada suhu dingin. Bakso direndam pada asap cair 0.8% selama 30 menit dan dipasteurisasi pada suhu ±88ºC selama 30, 60 dan 90 menit, lalu dikemas vakum dan disimpan pada suhu dingin. Berdasarkan nilai hasil pengamatan Angka lempeng Total, bakso ikan dapat bertahan selama 10 hari dengan nilai ALT tertinggi 6.3 x 104 CFU/g dan masih dapat diterima SNI 7266:2014. Total Salmonella sp sampai pada hari ke 20 memiliki nilai negative dan Total Koliform dan E. coli tidak terdeteksi pertumbuhan sampai pada penyimpanan dingin selama 30 hari. Nilai kadar air bakso ikan tinggi dan pH yang baik untuk pertumbuhan bakteri. Perendaman dalam asap cair menunjukkan bahwa asap cair merupakan pengawet antibakteri yang baik untuk digunakan sebagai bahan tambahan. Kata Kunci: Bakso Ikan, Asap Cair, Pasteurisasi, Bakteri.

scholarly journals KAJIAN MUTU DAN DAYA SIMPAN DENDENG UDANG PUTIH (Penaeus merguensis) SELAMA PENGEMASAN DAN PENYIMPANAN SUHU RUANG

AGROINTEK ◽  
2021 ◽  
Vol 15 (2) ◽  
pp. 608-616
Author(s):  
Yulia Delviani ◽  
Susi Lestari ◽  
Shanti Dwita Lestari ◽  
Sherly Ridhowati
Keyword(s):  
Room Temperature ◽  
White Shrimp ◽  
Plate Count ◽  
Total Volatile ◽  
Paired Sample ◽  
Total Plate Count ◽  
Average Water ◽  
Storage Times ◽  
And Storage ◽  
Total Yeast

The white shrimp (Penaeus merguensis)is abundant in Indonesia which could be made the jerky product to increase its shelflife. This study was aimed to determine the quality and shelflife of vacuum and non- vacuum  packed white shrimp jerky stored at room temperature. And,  the packed of white shrimp jerky was stored at room temperature for 0, 3, 6 and 9 days. The data obtained were analyzed using paired sample t-test. The condition of packaging had affected the quality and storage times for this jerky. The average water content  value was 23,01% (dry basis,db) for all the treatments. For 9 days, the TVB-N (total volatile based- nitrogen) and TPC (total plate count) were 22,41% mgN/100g and 3,27 log CFU/g (vacuum packed), then 26,52 mgN/100g and 6.12 log CFU/g (non-vacuum packed), respectively. During storage,  total yeast and fungi, as well as  aw (activity water), have increased. The vacuum- packaged white shrimp jerky was the best option for storing this product, and it has a shelflife of up to 9.

scholarly journals Effect of Humidity-Controlled Dehydration on Microbial Growth and Quality Characteristics of Fresh Wet Noodles

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 844
Author(s):  
Jun-Jie Xing ◽  
Dong-Hui Jiang ◽  
Zhen Yang ◽  
Xiao-Na Guo ◽  
Ke-Xue Zhu
Keyword(s):  
Shelf Life ◽  
Microbial Growth ◽  
Treatment Time ◽  
Positive Influence ◽  
Quality Characteristics ◽  
Plate Count ◽  
Cooking Properties ◽  
Total Plate Count ◽  
Different Temperatures

Humidity-controlled dehydration (HCD) was innovatively applied in this paper to control the growth of microorganisms in fresh wet noodles (FWN). Effects of HCD treatment with different temperatures (40, 60 or 80 °C), relative humidity (RH, 50%, 70% or 90%) and treatment time (5–32 min) on the total plate count (TPC), the shelf-life, and qualities of FWN were investigated. The results showed that HCD reduced the initial microbial load on the fresh noodles and extended the shelf-life up to 14–15 days under refrigeration temperature (10 °C). A 1.39 log10 CFU/g reduction for the initial TPC was achieved after HCD treatment at the temperature of 60 °C and RH of 90%. HCD with higher RH had a more positive influence on quality improvement. The L* values, the apparent stickiness, and the cooking properties of the noodle body were improved by HCD while good sensory and texture quality of noodles were still maintained after the dehydration process.

scholarly journals AKTIVITAS ANTIMIKROBA YOGURT BERBASIS AIR KELAPA MENGHAMBAT BAKTERI PATOGEN SECARA IN VITRO

1970 ◽  
Vol 17 (2) ◽  
Author(s):  
Lindawati S. A. ◽  
Haniyah Y. S. ◽  
Miwada I N. S. ◽  
Inggriati N. W. T. ◽  
Hartawan M. ◽  
...  
Keyword(s):  
Salmonella Typhi ◽  
Lactobacillus Bulgaricus ◽  
Plate Count ◽  
Klebsiella Pneumonia ◽  
E Coli ◽  
Total Plate Count

Yogurt merupakan minuman susu yang difermentasi dengan menggunakan bakteri asam laktat Lactobacillus bulgaricus dan Streptococcus thermophyllus sebagai starter. Tujuan penelitian untuk mengetahui kemampuan tertinggi dari yogurt berbasis air kelapa dalam menghambat pertumbuhan bakteri pathogen (Salmonella, Staphylococcus, Klebsiela pneumonia dan E.coli) secara in vitro. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan dan empat ulangan. Keempat perlakuan tersebut: Ko: susu skim 12%+air; K1: susu skim 12%+air kelapa gading; K2: susu skim 12%+kelapa gading bulan; K3: susu skim 12%+air kelapa hijau. Peubah yang diamati adalah aktivitas antimikroba yogurt terhadap bakteri pathogen (Salmonellatyphi, Staphylococcus, Klebsiella pneumonia dan E.coli), total plate count (TPC), total bakteri asam laktat dan E,coli. Hasil penelitian menunjukkan bahwa aktivitas antimikroba tertinggi diperoleh pada yogurt berbasis air kelapa bulan terhadap bakeri Salmonella typhi dan Staphylococcus masing-masing sebesar 0,21 dan 0,26 cm dengan total bakteri asam laktat1,1×107 cfu/mL sedangkan untuk Klebsiella pneumonia dan E.coli diperoleh pada yogurt berbasis air kelapa gading sebesar 0,38 dan 0,64 mm dengan total bakteri asam laktat 3,1×106 cfu/mL.Dari hasil penelitian dapat disimpulkan bahwa yogurt berbasis air kelapa gading, bulan, hijau dan tanpa air kelapa memiliki aktivitas antimikroba berspektrum luas (menghambat bakteri gram positif dan negatif).

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