scholarly journals Efek subletal campuran ekstrak daun srikaya (Annona squamosa L.) dan rimpang kunyit (Curcuma domestica Val.) terhadap larva Spodoptera litura F.

Agromix ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 66-78
Author(s):  
Ramadhan Taufika ◽  
Siti Sumarmi ◽  
Setyo Andi Nugroho

Spodoptera litura (Lepidoptera: Noctuidae) larvae are polyphagous insects whose populations cause damage to various types of plantations, horticulture, and food so that control efforts need to be done. One way to control the environment of environmentally friendly S. litura larvae is to use a plant-based insecticide, a mixture of A. squamosa leaf extract and C. domestica rhizome (test extract appendix). The purpose of this study was to determine the subletal effect of the test extract mixture on S. litura larvae, namely the duration of larvae and pupae stage, pupae weight, morphology and imago viability, and the ability of female imago to lay eggs to produce offspring. The test was carried out by the method of feed dip into the test extract mixture. Data on pupae weight were analyzed with ANOVA, followed by Duncan's further test with a 5% confidence level using SPSS 21 program. The results showed that the test extract mixture extended the stages of larvae and pupae, reduced pupa weight, imago wings did not develop fully, reduced imago size, and did not imago able to produce eggs. The results of this study could then be further tested on a greenhouse scale and photochemical analysis of a test extract mixture to determine bioactive compounds that act as plant-based insecticides.

2020 ◽  
Vol 26 (1) ◽  
pp. 32-41
Author(s):  
Ramadhan Taufika ◽  
Setyo Andi Nugroho ◽  
Anni Nuraisyah

Spodoptera litura F. larvae (Lepidoptera: Noctuidae) are polyphagous insects that cause damages to many types of plantations and horticulture that need to be protected. One of environmentally friendly method to protect ecological balance is the use of botanical insecticides whose bioactive compounds come from plant extracts. This study used a mixture of two plant extracts from A. squamosa leaves extract and C. domestica rhizome exstract (the mixed of experimental extracts). The mixed of experimental extracts are synergistic so that they are more effective and need a lower concentration to cause the mortality of S. litura larvae than the use of a single extract. The aims of the research were to know the efficacy of the mixed of experimental extracts on the mortality of S. litura larvae and determine the value of LC50 and LC90 extract test on its mortality. The initial and efficacy test were conducted by bait dip into the mixed of experimental extracts. The mortality data were analyzed using ANAVA, then continued by Duncan test and Probit analysis. The results of this research showed that the mixed of experimental extracts were effective to kill S. litura larvae. The calculated result of LC50 and LC90 showed that the LC50 value of the experimental extracts on the second and the third instar larvae were 5.252 ppm and 10.559 ppm, respectively, and the LC90 value for both instar larvae were 11.124 ppm and 13.638 ppm, respectively. It could be concluded that the mixed of experimental extracts is effective in S. litura larvae mortality and more effective in the mortality of the second instar larvae than that of the third instar larvae. Therefore, this test extract mixture can be developed further in a greenhouse to evaluate the effectiveness in the mortality of S. litura larvae which can be used as an alternative to controlling S. litura larvae with an environmentally friendly material in farmers.   Keywords: control, extract, mortality, Spodoptera litura


bionature ◽  
2018 ◽  
Vol 18 (2) ◽  
Author(s):  
A.Irma Suryani ◽  
Nova Hariani ◽  
Ahmad Fudhail Majid ◽  
Siti Risqa

Abstract. Caterpillar grayak (Spodoptera litura) is one of the pests in various types of vegetable plants are polyphagous and have a wide range of hosts. One common way of controlling farmers to overcome Spodoptera litura attack is to use insecticide. The use of insecticides that are not rational will accelerate the occurrence of pest resistance against insecticides. An alternative is needed to develop biological products which are generally narrow-spectrum chemicals to target organisms. Flowers at four (Mirabilis jalapa) contain bioactive compounds that are toxic. M. Jalapa contains some secondary metabolite compounds such as saponins, flavonoids, tannins, and polyphenols. Based on the description above it is necessary to conduct a research to determine the percentage mortality of caterpillar grayak on giving the leaf extract at four o'clock flowers. The method used is leaf dipping methods and analysis with POLO-PC software. The results showed that 1.6% concentration of extract of M. jalapa can kill the number of larvae as much as 30 tails with a percentage of 75% within 48 hours. The LC50 value of mortality of M. jalapa extract on Spodoptera litura larvae was 0,507% (0,378-0,661 with p <0,05) with slope1,516 ± 0,318.Keywords: Bioinsecticides, Mirabilis jalapa, Spodoptera litura, Mortality 


Author(s):  
P. S. Ajiningrum ◽  
I. A. K. Pramushinta

Red spinach (Alternanthera amoena) is one of the vegetable commodities that many people in demand for consumption because of its high nutritional content. Efforts to increase the production of red spinach often experience various obstacles. The main obstacle that limits the productivity of red spinach in the tropics is the number of Plant Disturbing Organisms  in the form of pests and potential diseases that can attack and decrease the production of spinach. One of the pests that often attack spinach is the existence of army worm (Spodoptera litura F.). Therefore, farmers need a way to eradicate the pest. This research is experimental using Random Complete Group. The data obtained were analyzed with variant analysis (ANOVA) in one direction and continued with LSD (Least Significant Different) test. Provision of botanical insecticide done by spraying the extract of leaves and seeds mimba with concentration dose 0%, 5%, 10% and 15%. In the final analysis of mortality data of army worm (Spodoptera litura F.) treated with mimba leaf extract obtained significant value of 76.263 at a significant level of 0.05 so it can be concluded that there is influence of mimba leaf extract on mortality of army worm. In the final analysis of mortality data of army worm (Spodoptera litura F.) treated with mimba seed extract obtained significant value of 78.625 at a significant level of 0.05 so it can be concluded that there is an effect of mimba seeds extract on mortality army worm.   Keywords: mimba leaf extract,  mimba seed extract, red spinach (Alternanthera amoena), army worm (Spodoptera litura F.), botanical insecticide


2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Gurmehar Kaur Grewal ◽  
Neelam Joshi ◽  
Yadhu Suneja

Abstract Background Spodoptera litura (Fab.) (Lepidoptera: Noctuidae) is a serious agricultural pest that infests many commercially important crops of Southeast Asian countries. Indiscriminate use of chemical pesticides has led to various health hazards as well as insecticide resistance. Entomopathogenic fungi (EPF) provide an important alternative as biological control agents. Metarhizium rileyi is an EPF with a specific host range for lepidopteran pests. The present study aimed to identify virulent M. rileyi isolate against S. litura larvae and analyse their extracellular cuticle-degrading enzyme activities. Results Three M. rileyi isolates viz M. rileyi NIPHM, M. rileyi MTCC 4254 and M. rileyi MTCC 10395 formulations were evaluated at different concentrations against 2nd instar larvae of S. litura. A maximum percent mortality of 63.33% was recorded in M. rileyi NIPHM (12 g/l), followed by M. rileyi MTCC 4254 (58.33%) at the same concentration, 10 days post-treatment. Maximum means of chitinase, protease and lipase activities (0.44, 1.58 and 2.95 U/ml, respectively) were recorded in the case of M. rileyi NIPHM. Correlation analysis was positive between enzyme activity and larval mortality. Conclusions Metarhizium rileyi NIPHM recorded the highest enzymatic activity and exhibited the maximum mortality rate against 2nd instar larvae of S. litura, suggesting the possible role of these enzymes in the pathogenicity of the fungus. Further knowledge in this regard may help in the development of enzyme-based screening methods for selecting virulent fungal isolates for the eco-friendly management of crop pests.


Author(s):  
Parameswari P ◽  
Devika Rengaswamy

<p>ABSTRACT<br />Objective: The points of this exploration work were to decide the quantitative examination of bioactive mixes. Customarily, cutting edge meds rely<br />on the phytochemicals got from the plant source in bigger extents. Numerous bioactive auxiliary metabolites have a positive metabolic reaction on<br />different human diseases.<br />Methods: In the present examination, Artemisia nilagirica, leaves were gathered, dried, powdered and put away in hermetically sealed compartments<br />for quantitative investigation of phytochemicals according to standard strategies.<br />Results: The methanolic leaf concentrate of enrolled 4.33 mg of alkaloids, 1.22 mg of saponins, 12.4 mg of tannins, 24.3 mg of glycosides, 10.2 mg<br />terpenoids, 1.33 mg of coumarin, 59.4 mg of amino acids, 12.2 mg of fatty acids, 17.2 mg of flavonoids, 10.2 mg of phenols, and steroids in follows<br />separately.<br />Conclusion: The plant has a high helpful quality as far as an assortment of phytochemicals from leaf remove and had let to a sure level toward<br />extraction and refinement of specific bioactive mixes for human nourishment.<br />Keywords: Artemisia nilagirica, Secondary metabolites, Quantitative analysis, Leaf extract, Flavonoids.</p>


2012 ◽  
Vol 41 (6) ◽  
pp. 503-509 ◽  
Author(s):  
G D Rossi ◽  
C D Santos ◽  
G A Carvalho ◽  
D S Alves ◽  
L L S Pereira ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document