scholarly journals Analisis Fitokimia Dan Toksisitas Dari Ekstrak Daun Tumbuhan Mempening (Lithocarpus bancanus (Scheff.) Rehd) Menggunakan Metode BSLT (Brine Shrimp Lethality Test)

2019 ◽  
Vol 2 (2) ◽  
pp. 29-33
Author(s):  
Retno Puji Lestari ◽  
Rudi Hendra Sy ◽  
Hilwan Yuda Teruna
Keyword(s):  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
The Family ◽  
Tribal Region

Mempening (Lithocarpus bancanus (Scheff.) Rehd) is a species belongs to the family Fagaceae which is found growing around the Talang Mamak tribal region in Kelayang District, Indragiri Hulu Regency, Riau Province. The purpose of this study was to determine the toxicity of leaf extract of L. bancanus (Scheff.) Rehd against Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method and its phytochemical analysis. The results of the toxicity was determined against n-hexane, dichloromethane, ethyl acetate, methanol, and ethanol extract and they showed LC50 values ​​of 3.15 ppm; 41163.26 ppm; 5817.09 ppm; 817, 27 ppm; 9019.70 ppm. Phytochemical analysis of the leaves of L. bancanus (Scheff.) Rehd plant showed a class of flavonoids, terpenoids, phenolics and saponins.

scholarly journals KAJIAN TOKSISITAS DARI TUBUH BUAH Ganoderma lucidum DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST)

2014 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
Ross Nurul Rohmah ◽  
Nuniek Ina Ratnaningtyas ◽  
Ari Asnani
Keyword(s):  
Toxic Effect ◽  
Fruiting Body ◽  
Brine Shrimp ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
One Stage ◽  
Body Extract

Ganoderma lucidum is polyporus fungi from Basidiomycetes which can be used as traditional medicines. Toxicity test with Brine Shrimp Lethality Test (BST) method using Artemia salina was conducted to find out toxic effect of G.lucidum. An extract would have toxic effect if the LC50 < 1000 µg/ml. The aims of this experiment were to know about the toxicity level from G.lucidum with Brine Shrimp Lethality Test (BST) method and determined the concentration of fruiting body extract of G.lucidum which had the best toxicity effect in LC50. There were two extraction methods used in this experiment, first one stage extraction and then multilevel extraction with hexane, ethyl acetate, and ethanol. Each solvent was made in 1000 ppm, 500 ppm, 250 ppm, and 125 ppm. This process was repeated for three times. This experiment used G.lucidum extract which was tested to A.salina and secunder compound metabolit test from the most toxic G. lucidum was done with phytochemical analysis. The result indicated that fruiting body extract from G.lucidum could kill A.salina because all extract was positively contained alkaloid and terpenoid but negatively contained flavonoid. Fruiting body extract from G.lucidum which had lowest LC50 wass ethyl asetat extract with one stage extraction in concentration 53,70 ppm and highest LC50 was ethanol extract with multilevel extraction in concentration 501,18 ppm.

scholarly journals A Cytotoxic Activity of Tahongai (Kleinhovia hospita Linn.) Leaves Extracts Using Brine Shrimp Lethality Test

2019 ◽  
Vol 4 (3) ◽  
pp. 60
Author(s):  
Indah Solihah ◽  
Herlina Herlina ◽  
Riana Sari Puspita Rasyid ◽  
Tri Suciati ◽  
Khairunnisa Khairunnisa
Keyword(s):  
Statistical Analysis ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Ethanol Extracts

Tahongai (Kleinhovia hospita Linn.) leaves had been known contain alkaloids, flavonoids, saponins, steroids, and tannins. At Komering, South Sumatera tribe, tahongai leaves had been known to treat tumor, cancer, polyps, acne, and dysmenorrhea. The study of cytotoxic activity of tahongai bark and stem were done. This study aims to determine the cytotoxic activity of tahongai leaves extracts using BSLT method. Tahongai leaves were extracted using gradual maceration with n-hexane, ethyl acetate, and ethanol 96%. Each extract was tested cytotoxic activity towards Artemia salina L. larvae. The yield of n-hexane, ethyl acetate, and ethanol extracts are 2,686%, 7,033%, and 7,933% respectively. Ethanol extract of tahongai leaves had the best cytotoxic activity with lethality value 76,667% at 500ppm. Statistical analysis with two way ANOVA showed extract and concentration had a significant (p<0,05) effect on larvae lethality percentage.

scholarly journals UJI TOKSISITAS EKSTRAK DAUN HYPERICUM LESCHENAULTII CHOISY DARI GUNUNG LAWU TERHADAP ARTEMIA SALINA L. SEBAGAI KANDIDAT ANTIKANKER

At-Taqaddum ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 168
Author(s):  
Yudha Noviana ◽  
Dwimei Ayudewandari Pranatami
Keyword(s):  
Medicinal Plants ◽  
Bioactive Compounds ◽  
Brine Shrimp ◽  
Leaf Extract ◽  
Biological Activities ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Rf Values ◽  
Layer Chromatography

<p><em>Mount Lawu is a biodiversity rich mountain, with the potensial of medicinal plants of various kinds including anticancer plants. Flora on the Mount Lawu that has potential for anticancer is Hypericum leschenaultii Choisy containing compounds hypericin. Hypericum has several biological activities such as a antioxidant, antitumor, and anticancer. The research aims to determine the potential of bioactive compounds and extracts of the leaves of H. leschenaultii from Mount Lawu and toxicity to Artemia salina Leach and profile Thin Layer Chromatography (TLC) it.   Ethanol extract of the leaves of H. leschenaultii was tested toxicity with Brine Shrimp Lethality Test (BST) using a concentration of  400 µg/ml, 200 µg/ml, 100 µg/ml, 50 µg/ml, 25 µg/ml. The TLC profiles of bioactive compounds were determined by using spesific spray reagent.  The results show the potential and toxicity of leaf extract of H. leschenaultii with LC values 175,77 µg/ml. TLC profile of bioactive compounds leaf extract of H. leschenaultiicontaining flavonoids with Rf values of 0,98 and terpenoid compounds with Rf value of 0,91; 0,95; 0,98. </em></p><p>==================================================</p><p>Gunung Lawu merupakan gunung yang kaya akan keanekaragaman hayati,  dengan potensi tumbuhan obat yang beragam, termasuk potensi tumbuhan sebagai antikanker.  Adanya potensi tumbuhan sebagai sumber senyawa antikanker tersebut belum banyak diteliti dan digunakan sebagai pengobatan alternatif.  Flora di Gunung Lawu yang potensial sebagai tumbuhan antikanker, yaitu <em>Hypericum leschenaultii </em>Choisy yang mengandung senyawa <em>hypericin</em> dan memiliki status keberadaan yang melimpah di Gunung lawu, sehingga sangat penting untuk diketahui manfaatnya agar dapat dimanfaatkan lebih luas.  <em>Hypericin </em>memiliki beberapa aktivitas biologis seperti antioksidan, antitumor, dan antikanker. Penelitian ini bertujuan untuk mengetahui potensi senyawa bioaktif ekstrak daun <em>H. leschenaultii </em>dari Gunung Lawu sebagai antikanker dengan melihat toksisitasnya terhadap <em>Artemia salina </em>Leach serta profil Kromatografi Lapis Tipis (KLT) nya. Untuk mengetahui potensi sebagai tanaman antikanker maka ekstrak etanol daun <em>H. leschenaultii</em> diuji toksisitasnya dengan metode <em>Brine Shrimp Lethality Test </em>(BST). Dalam uji BST tersebut digunakan 5 konsentrasi esktrak etanol daun <em>H. leschenaultii </em>yaitu:<em> </em>400 µg/ml, 200 µg/ml, 100 µg/ml, 50 µg/ml, 25 µg/ml. Kandungan senyawa bioaktif dideteksi dengan reagen semprot spesifik untuk mengetahui profil KLT-nya. Hasil penelitian menunjukkan potensi dan toksisitas ekstrak <em>H. leschenaultii</em> dengan nilai LC 175,77 µg/ml. Profil KLT senyawa bioaktif ekstrak daun <em>H. leschenaultii</em> mengandung senyawa flavonoid dengan nilai Rf 0,98 serta senyawa terpenoid dengan nilai Rf 0,91; 0,95; 0,98.</p><p> </p>

scholarly journals SKRINING FITOKIMIA, UJI AKTIVITAS ANTIOKSIDAN, DAN TOKSISITAS DARI EKSTRAK DAUN KERSEN (Muntingia calabura L.) DENGAN METODE 1.1-diphenyl-2-picrylhydrazyl (DPPH) dan Brine Shrimp Lethality Test (BSLT)

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 315
Author(s):  
Selin Widjaya ◽  
Widdhi Bodhi ◽  
Adithya Yudistira
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Brine Shrimp Lethality ◽  
Phytochemical Content ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Dpph Method ◽  
Muntingia Calabura

ABSTRACTKersen (Muntingia calabura L.) is a plant that has begun to be eliminated and was rarely used because it is often considered to have no economic value and lack of knowledge about its utilization, whereas kersen plants contain flavonoids, saponins, and tannins which were have high benefit for health. The content of metabolites is affected by soil nutrient elements and difference place of growth. This study aims to determine the potential of kersen leaves grown in North Minahasa based on phytochemical content, ability of antioxidant activity, and toxicity. Kersen leaves were extracted using sequential maceration method with n-hexane, ethyl acetate, and ethanol as solvents. Phytochemical Screening using several reagents which tailored to the type of phytochemical test. 1.1-diphenyl-2-picrylhydrazyl (DPPH) method is used to evaluate antioxidant activity, and Brine Shrimp Lethality Test (BSLT) method is used to evaluate toxicity. The result of this study indicate that the n-hexane extract contains phenols, flavonoids, and tannins, with IC50 value 12.54 μg/mL, and LC50 value 881 μg/mL. Ethyl acetate extract contains phenols, flavonoids, tannins, and saponins, with IC50 value 61.3 μg/mL, and LC50 value 1758 μg/mL. Ethanol extract has phenol, flavonoid, tannin, saponin, and terpenoid content, with IC50 value 9.01 μg/mL, and LC50 value 106 μg/mL. Keywords : Kersen leaves, Antioxidant, Toxicity, IC50, LC50 ABSTRAKKersen (Muntingia calabura L.) merupakan tanaman yang sudah mulai tersingkirkan dan jarang dimanfaatkan karena sering dianggap tidak punya nilai ekonomis dan kurangnya pengetahuan tentang pemanfaatannya, padahal tanaman kersen memiliki kandungan flavonoid, saponin, dan tanin yang bermanfaat tinggi untuk kesehatan. Kandungan senyawa metabolit dipengaruhi oleh unsur hara tanah dan perbedaan tempat tumbuh. Penelitian ini bertujuan untuk mengetahui potensi dari daun kersen yang tumbuh di Minahasa Utara berdasarkan kandungan fitokimia, kemampuan aktivitas antioksidan, dan toksisitasnya. Ekstrak daun kersen diekstraksi dengan metode maserasi sekuensial menggunakan pelarut n-heksan, etil asetat, dan etanol.  Skrining fitokimia menggunakan beberapa reagen yang disesuaikan dengan jenis uji fitokimia. Metode 1.1-diphenyl-2-picrylhydrazyl (DPPH) digunakan untuk mengevaluasi aktivitas antioksidan, dan metode Brine Shrimp Lethality Test (BSLT) digunakan untuk mengevaluasi toksisitas. Hasil penelitian ini menunjukkan bahwa ekstrak n-heksan memiliki kandungan fenol, flavonoid, dan tanin, nilai IC50 12,54 μg/mL, dan nilai LC50 881 μg/mL. Ekstrak etil asetat memiliki kandungan fenol, flavonoid, tanin, dan saponin, nilai IC50 61,3 μg/mL, dan nilai LC50 1758 μg/mL. Ekstrak etanol memiliki kandungan fenol, flavonoid, tanin, saponin, dan terpenoid, nilai IC50 9,01 μg/mL, dan nilai LC50 106 μg/mL. Kata kunci : Daun Kersen, Antioksidan, Toksisitas, IC50, LC50

scholarly journals Toxicity Test of Ethanol Extract Ant Plant Local Aceh (Mymercodia sp) Method of BSLTLarvae Shrimp Artemia salina Leach

2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Frengki F ◽  
Roslizawaty R ◽  
Desi Pertiwi
Keyword(s):  
Crude Extract ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Rotary Evaporator

Toxicity test and phytochemical screening of ant plant ethanol extract were done. The toxicity test was done by employing the Brine Shrimp Lethality Test (BSLT) method. Sample of ant plant taken from the Aceh Besar forest have been extracted with ethanol. The sample was thenconcentrated using a rotary evaporator until a crude extract was obtained. The extract was tested by toxicity to larva Artemia salina L. Thetoxicity test for larvae Artemia salina Leach have been done by employing the Brine Shrimp Lethality Test (BSLT) method at four concentrationlevels, i.e: 250 ppm, 125 ppm, 50 ppm, and 25 ppm. The result of the research showed that ethanol extract of ant plant Aceh had acute toxicityby LC value 61.11 ppm. The extract had identified were fenolik, saponin, steroid and terpenoid.____________________________________________________________________________________________________________________Key words: rine Shrimp Lethality Test, LC5050, Ant Plant, Aceh, Bphytochemical Screening 

scholarly journals Uji Toksisitas Akut Ekstrak dan Fraksi Kulit Batang Ketapang Laut (Terminalia Catappa L.) Menggunakan Metode BSLT

2020 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Ari Sartinah ◽  
Yamin Yamin ◽  
Muhammad Arba ◽  
Nur Illiyyin Akib ◽  
Andi Nafisah Tendri Adjeng ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Program Analysis ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Brine Shrimp Lethality ◽  
Terminalia Catappa ◽  
Water Fractions

AbstrakToksisitas akut merupakan kemampuan suatu bahan kimia (obat/bahan obat) dalam menimbulkan kerusakan  pada suatu organisme dalam waktu yang relatif singkat. Tujuan penelitian ini adalah untuk mengetahui potensi ketoksikan akut dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L.). Pengujian toksisitas akut dilakukan dengan menggunakan metode BSLT (Brine Shrimp Lethality Test) dan menggunakan analisis program untuk mengetahui nilai LC50 dari ekstrak dan fraksi. Hasil uji toksisitas akut menunjukan nilai LC50  dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L. )  berturut-turut 247,997 ppm; 400,666 ppm dan 618,046 ppm. Hal ini menunjukan bahwa ekstrak metanol batang ketapang laut (Terminalia catappa L.) bersifat paling toksik dibandingkan fraksi etil asetat dan fraksi air.Kata kunci: Ekstrak, Fraksi, Terminalia catappa L., BSLT, Artemia salina LeachAbstractAcute toxicity is the ability of chemical (drug/drug ingredient) to cause damage to organisms in a relatively short time. The purpose of this study was to determine the potential for acute toxicity of methanol extract, ethyl acetate and water fractions of sea ketapang stem skin (Terminalia catappa L.). Acute toxicity testing was performed using the BSLT (Brine Shrimp Lethality Test) method and program analysis to determine the LC50 values of extracts and fractions. The results of the acute toxicity test showed the LC50 values of methanol extract, ethyl acetate and water fractions of the plant stem skin consecutively were 247,997 ppm; 400,666 ppm and 618,046 ppm. This shows that the methanol extract of sea ketapang stems (Terminalia catappa L.) is the most toxic compared to the ethyl acetate and the water fraction.Keywords: Extract, Fraction, Terminalia catappa L., BSLT, Artemia salina Leach

scholarly journals ANALISIS SENYAWA METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK ETANOL BATANG TANAMAN PATAH TULANG (Euphorbia tirucalli L.) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2014 ◽  
Vol 14 (2) ◽  
pp. 106 ◽  
Author(s):  
Grace S. Baud ◽  
Meiske S. Sangi ◽  
Harry S.J. Koleangan
Keyword(s):  
Secondary Metabolite ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Euphorbia Tirucalli ◽  
Ethanol Extracts

ABSTRAK Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman Patah tulang (Euphorbia tirucalli L.). Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia yaitu senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak segar dan kering batang tanaman Patah tulang. Penentuan toksisitas ekstrak etanol batang tanaman Patah tulang menggunakan metode Brine Shrimp Lethality Test (BSLT). Uji toksisitas digunakan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh positif flavonoid, tanin dan steroid sedangkan alkaloid, saponin dan triterpenoid negatif. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan metode probit menggunakan perangkat lunak SPSS 20.0 (untuk sistem operasi Windows). Hasil uji toksisitas ekstrak etanol batang Patah tulang menunjukkan bahwa ekstrak segar maupun kering bersifat sitotoksik dengan nilai LC50 (7,994ppm) untuk ekstrak etanol batang segar  dan LC50 (9,940ppm) untuk ekstrak etanol batang kering. Kata kunci : Euphorbia tirucalli L., Senyawa metabolit sekunder, Toksisitas, Artemia salina Leach, LC50.   ANALYSIS OF SECONDARY METABOLITE COMPOUNDS AND TOXICITY TEST OF  STEM  PLANT ETHANOL EXTRACTS OF  PATAH TULANG (Euphorbia tirucalli L.) BY BRINE SHRIMP LETHALITY TEST’S METHOD (BSLT) ABSTRACT A research on the analysis of secondary metabolite compounds contained in the stem of  patah tulang (Euphorbia tirucalli L.) and its toxicity had been done. The analysis was performed through phytochemical screening of alkaloids, flavonoids, triterpenoids, steroids, saponins and tannins on extracts of fresh and dried stem of the plant. Toxicity of ethanol extract of the stem was determined using Brine Shrimp Lethality Test (BSLT) method with Artemia salina Leach as bioindicator. Phytochemical screening showed positive results for flavonoids, tannins, and steroids and negative one for alkaloids, saponins, and triterpenoids. Toxicity data were obtained from Lethality Concentration 50 (LC50) calculation trhrough probit analysis using SPSS 20.0 software (for Windows operating systems). Toxicity test showed that ethanol extracts of the fresh and dried stem were cytotoxic with LC50 value of 7.994 ppm and 9.940 ppm, respectively. Keywords: Euphorbia tirucalli L., Secondary metabolite compounds, Toxicity, Artemia salina Leach, LC50.

scholarly journals SKRINING FITOKIMIA DAN UJI TOKSISITAS EKSTRAK DAUN PECUT KUDA (Stachytarpheta jamaicensis L. Vahl) TERHADAP LARVA UDANG Artemia salina Leach

2006 ◽  
Vol 12 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Lany Indrayani ◽  
Hartati Soetjipto ◽  
Lydia Sihasale
Keyword(s):  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Leaf Extract ◽  
Ethyl Acetate Fraction ◽  
Artemia Salina ◽  
Hexane Fraction ◽  
Chloroform Fraction ◽  
Phytochemical Screening ◽  
Chemical Substances ◽  
Toxicity Effect

Phytochemical screening and Brine Shrimp lethality test of pecut kuda leaf extract had been done against Artemia salina Leach. The aims of this study is to screen potentially bioactive extract of pecut kuda leaf as an effort ti find out the chemical substances responsible for the toxic effect. The Pecut kuda leaves (Stachytarpheta jamaicensis, L. Vahl) was extracted by methanol 80 percent, then partitioned successively with hexane, chloroform, and ethyl acetate. Phytochemical screening was done according to Ciulei method (1984). The toxicity effect was tested against A. salina, L (Brine Shrimp Test). The result of phytochemical screening known that compounds in the extract of pecut kuda leaves (S. jamaicensis, L. Vahl), hexane fraction contained of sterol and triterpenes; where the chloroform fraction contained of saponin, sterol, and triterpenes; further more the ethyl acetate fraction contained flavonoid, tannin, saponin, sterol, dan triterpenes. The result of the study shows that several fractions of extract of pecut kuda leaves (S. jamaicensis) hexane fraction, chloroform, and ethyl acetate was toxic because it was able to kill more than 50 percent larva of A. salina, L at the concentration less than 1000 ppm. The LC50 of all fractions of pecut kuda leaves extract (S. jamaicensis, L. Vahl) were shown from the most toxic to the lowest were as followed: LC50 of hexane fraction = 98.33 ppm, of chloroform raction LC50 = 204.17 ppm, and of ethyl acetate fraction LC50 = 249.80 ppm. Finally results of the TLC profile showed that all fraction (hexane, chloroform, and ethyl acetate) indicated that the pecut kuda leaves extract (S. jamaicensis) contained terpenoid compound.

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