scholarly journals Uji Toksisitas Akut Ekstrak dan Fraksi Kulit Batang Ketapang Laut (Terminalia Catappa L.) Menggunakan Metode BSLT

2020 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Ari Sartinah ◽  
Yamin Yamin ◽  
Muhammad Arba ◽  
Nur Illiyyin Akib ◽  
Andi Nafisah Tendri Adjeng ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Program Analysis ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Brine Shrimp Lethality ◽  
Terminalia Catappa ◽  
Water Fractions

AbstrakToksisitas akut merupakan kemampuan suatu bahan kimia (obat/bahan obat) dalam menimbulkan kerusakan  pada suatu organisme dalam waktu yang relatif singkat. Tujuan penelitian ini adalah untuk mengetahui potensi ketoksikan akut dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L.). Pengujian toksisitas akut dilakukan dengan menggunakan metode BSLT (Brine Shrimp Lethality Test) dan menggunakan analisis program untuk mengetahui nilai LC50 dari ekstrak dan fraksi. Hasil uji toksisitas akut menunjukan nilai LC50  dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L. )  berturut-turut 247,997 ppm; 400,666 ppm dan 618,046 ppm. Hal ini menunjukan bahwa ekstrak metanol batang ketapang laut (Terminalia catappa L.) bersifat paling toksik dibandingkan fraksi etil asetat dan fraksi air.Kata kunci: Ekstrak, Fraksi, Terminalia catappa L., BSLT, Artemia salina LeachAbstractAcute toxicity is the ability of chemical (drug/drug ingredient) to cause damage to organisms in a relatively short time. The purpose of this study was to determine the potential for acute toxicity of methanol extract, ethyl acetate and water fractions of sea ketapang stem skin (Terminalia catappa L.). Acute toxicity testing was performed using the BSLT (Brine Shrimp Lethality Test) method and program analysis to determine the LC50 values of extracts and fractions. The results of the acute toxicity test showed the LC50 values of methanol extract, ethyl acetate and water fractions of the plant stem skin consecutively were 247,997 ppm; 400,666 ppm and 618,046 ppm. This shows that the methanol extract of sea ketapang stems (Terminalia catappa L.) is the most toxic compared to the ethyl acetate and the water fraction.Keywords: Extract, Fraction, Terminalia catappa L., BSLT, Artemia salina Leach

Toksisitas Akut Ektrak N-Heksan Dan Methanol Rimpang Jeringau Merah Terhadap Kematian Artemia Salina Leach Dengan Metode Brine Shrimp Lethality Test

2018 ◽  
Vol 1 (2) ◽  
pp. 125
Author(s):  
Edy Suwandi ◽  
Supriyanto Supriyanto
Keyword(s):  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Test Method ◽  
Endemic Plant ◽  
Brine Shrimp Lethality ◽  
Purposive Sampling ◽  
Study Results ◽  
Sweet Flag

Abstract: Acorus sp (red sweet flag) is an endemic plant from West Borneo which contains chemical substances such as flavonoids that have bioactivity as antioxidants so it is recommended one to prevent cancer. The study aimed to determine toxicity of n-Hexan extract and methanol red sweet flag rhizomes against Artemia salina Leach with Brine Shrimp Lethality Test method. This study was a type of quasi experimental design, sampling technique obtained by using purposive sampling. According to the study results, it can be seen than methanol extract from red sweet flag rhizomes give impact on the mortality average of Artemia salina Leach : concentration 100 µg/ml (51%), 250 µg/ml (75%), 500 µg/ml (92%), 750 µg/ml (96%) and 1000 µg/ml (99%). In addition, n-Hexan extract of red sweet flag rhizomes in 100 µg/ml (45%), 250 µg/ml (60%), 500 µg/ml (92%), 750 µg/ml (84%) and 1000 µg/ml (96%). It is reported based on probit analysis that LC50 of n-Hexan extract from red sweet flag rhizomes was 128.2921 µg/ml meanwhile methanol extract was 242.7169 µg/ml µg/ ml, thus in conclusion n-Hexan and methanol extract of red sweet flag rhizomes potentially cytotoxic to Artemia salina Leach larvae.Abstrak: Jeringau merah (Acorus sp) merupakan satu jenis tanaman endemik Kalimantan Barat memiliki kandungan kimia diantaranya flavonoidyang mempunyai bioaktivitas sebagai antioksidan sehingga sangat baik untuk pencegahan kanker. Penelitian ini bertujuan mengetahui mengetahui dan menentukan toksisitas ekstrak n-Heksan dan Methanol Rimpang Jeringau MerahterhadapArtemia salinaLeachdengan metode Brine Shrimp Lethality Test. Desain penelitian ini adalah eksperimental semu, teknik pengambilan sampel dengan cara purposive sampling. Berdasarkan hasil penelitian diketahui ekstrak Methanol Rimpang Jeringau Merah rerata kematianArtemia salina Leach konsentrasi 100 µg/ml (51%), 250 µg/ ml (75%), 500 µg/ml (92%), 750 µg/ml (96%) dan 1000 µg/ml (99%)pada ekstrak n-Heksan Rimpang Jeringau Merah konsentrasi 100 µg/ml (45%), 250 µg/ml (60%), 500 µg/ml (92%), 750 µg/ml (84%) dan 1000 µg/ml (96%). Dari analisis probit diketahuinilai LC50 ekstrak n-Heksan Rimpang Jeringau Merah yaitu 128.2921 µg/ml dan ekstrak Methanol Rimpang Jeringau Merah yaitu 242.7169 µg/ml µg/ml, maka disimpulkan ekstrak n-Heksan dan Methanol Rimpang Jeringau Merahbersifat sitotoksik terhadap larva Artemia salina Leach.

scholarly journals KANDUNGAN TOTAL FENOLIK DAN UJI TOKSISITAS DAUN MUHARANG BAWINE (Dendrophthoe falcate (Lf) Etinggsh) DENGAN METODE BLST (Brine Shrimp Lethality Test)

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Merlin . ◽  
Max Runtuwene ◽  
Vanda Kamu
Keyword(s):  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Hexane Extract ◽  
Test Method ◽  
Total Phenolic ◽  
Brine Shrimp Lethality ◽  
X 24

Penelitian ini bertujuan untuk,menentukan kandungan total fenolik, dan mengetahui tingkat toksisitas ekstrak muharang bawine (Dendrophthoefalcate (Lf) Etinggsh) dengan Metode BLST (Brine shrimp lethality test) dari beberapa pelarut.Uji toksisitas dengan menggunakan udang A. salina leach.Serbuk daun muharang bawine diekstraksi dengan cara maserasi bertingkat dengan menggunakan pelaut n-heksan, etil asetat dan metanol selama 3 x 24 jam sehingga diperoleh ekstrak kental. Penentuankandungan total fenolik diukur dengan metode Folin-Ciocalteu dan nilai LC50 dihitung dengan menggunakan SPSS 15.0.Kandungan total fenolik tertinggi terdapat pada hasil maserasi bertingkat ekstrak metanol, diikuti dengan hasil maserasi bertingkat ekstrak etil asetat dan hasil maserasi bertingkat ekstrak n-heksan. Hasil LC50 paling terbaik terdapat pada hasil maserasi bertingkat ekstrak metanol, kemudian diikuti dengan hasil maserasi bertingkat ekstrak etil asetat dan hasil maserasi bertingkat ekstrak n-heksan. ABSTRACT This study aims to determine the total phenolic content, and determine the toxicity level of muharang bawine extract (Dendrophthoe falcate (LF) Ettingsh) with the BLST (Brine Shrimp Lethality Test) method of several solvents. Toxicity test using A. salina Leach shrimp. Leaf Extract Muharang Bawine was extracted by multilevel maceration using n-hexane, ethyl acetate and methanol for 3 x 24 hours for thick extracts. Determination of total phenolic content was measured by the Folin-Ciocalteu method and LC50 valueswere calculated using SPSS 15.0. The highest total phenolic content was found in the results of maceration of methanol extract multilevel, followed by maceration results of multilevel ethyl acetate extract and maceration results of n-hexane extract. The best LC50 results were found in the results of maceration of methanol extract multilevel, then followed by maceration results of multilevel extract of ethyl acetate and maceration results of n-hexane extract. 

scholarly journals ANALISIS KANDUNGAN FITOKIMIA DARI UJI TOKSISITAS EKSTRAK METANOL DAUN SOYOGIK (Sauraula bracteosa DC) DENGAN MENGGUNAKAN METODE MASERASI

2013 ◽  
Vol 13 (2) ◽  
pp. 98 ◽  
Author(s):  
Morein A Maukar ◽  
Max R.J. Runtuwene ◽  
Julius Pontoh
Keyword(s):  
Brine Shrimp ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Test Method ◽  
Tannin Content ◽  
Toxicity Assay ◽  
Brine Shrimp Lethality ◽  
Antioxidants Activity

ANALISIS KANDUNGAN FITOKIMIA DARI UJI TOKSISITAS EKSTRAK METANOL  DAUN SOYOGIK (Sauraula bracteosa DC) DENGAN MENGGUNAKAN  METODE MASERASI ABSTRAK Penelitian ini bertujuan untuk mengetahui LC50 ekstrak metanol tanaman soyogik yang diekstraksi secara maserasi dan menganalisis kandungan fenolik. Analisis kandungan fitokimia meliputi uji fenolik, uji flavonoid dan uji tanin . Uji toksisitas menggunakan metode BSLT (Brine Shrimp Lethality Test) dengan menggunakan larva Artemia Salina Leach. Hasil yang diperoleh dan dianalisis  dengan analisis probit menggunakan S.PSS 20.0 untuk  mengetahui  nilai (LC50). Nilai LC50 yang diperoleh berdasarkan uji toksisitas daun soyogik yaitu sebesar 37,30  ppm. Kandungan fenolik, flavonoid  dan tanin berturut-turut adalah 43,06, 6,52 dan 17,91. Kata Kunci : Daun soyogik, Fitokimia, Toksisitas, Artemia salin FITOCHEMISTRY CONTENT ANALYSIS OF TOXICITY TEST OF METHANOL EXTRACT OF SOYOGIK LEAVE (Sauraula bracteosa DC) USING MASERATION METHOD   ABSTRACT The aims of this research are to measure the Lethal Concentration 50 (LC50) in toxicity activity  methanol extracts from soyogik plants that were extracted by maceration and to analyzing its phenolic content. Phytochemical screening include phenolic, flavonoid and tannin test.  Toxicity assay using BSLT (Brine Shrimp Lethality Test) method using Artemia Salina Leach. Results were analyzed by probit analysis using SPSS 20.0 for Windows to know the value of (LC50). Screening phytochemicals shows that there is antioxidants activity of Soyogik leaves. The LC50 values ​​were obtained by testing the toxicity of Soyogik leaves is equal to 37.301 ppm. The phenolic, flavonoid and tannin content were 43,06, 6,52 and 17,91, respectively. Keywords : Soyogik leaves, Phytochemical, Toxicity, Artemia salina.

scholarly journals UJI AKTIVITAS SITOTOKSIK EKSTRAK METANOL BUAH KETUMBAR (Coriandrum sativum Linn) TERHADAP ARTEMIA SALINA LEACH DENGAN UJI BSLT (Brine Shrimp Lethality Test)

2020 ◽  
Vol 2 (3) ◽  
pp. 137-146
Author(s):  
Mega Yulia ◽  
Rani Anggraini ◽  
Farizal Farizal
Keyword(s):  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Coriandrum Sativum ◽  
Brine Shrimp Lethality ◽  
Activity Test ◽  
Anti Cancer ◽  
Expected Benefits

Coriander is a common herb can be used as traditional medicine. One of the expected benefits of Coriander fruits is an anti-cancer. This study aims to determine the cytotoxic activity of Coriander extract (Coriandrum sativum Linn) on shrimp larvae (Artemia salina Leach) with the BSLT (Brine Shrimp Lethality Test) method. Coriander fruits was extracted using methanol solvent and maceration method, then concentrated it with a rotary evaporator until a thick extract. Cytotoxic activity test used methanol extract with several concentration : 1,000 ppm, 100 ppm, 10 ppm, and 1 ppm. The result of this research reveal that methanol extract of Coriander fruits has cytotoxic activity with LC50 value is 32.35 ppm.

scholarly journals Toksisitas Akut Ekstrak Metanol Mentimun (Cucumis Sativus L.) Terhadap Larva Artemia Salina Leach Metode Brine Shrimp Lethality Test (BSLT)

2019 ◽  
Vol 2 (2) ◽  
pp. 67
Author(s):  
Khairin Akbar Putra ◽  
Indah Purwaningsih ◽  
Kuswiyanto Kuswiyanto
Keyword(s):  
Acute Toxicity ◽  
Cucumis Sativus ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Early Screening ◽  
Microsoft Excel ◽  
Cucumis Sativus L ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

Abstract: Cucumber (Cucumis sativus L) is a plant that is widely consumed by the people. Beside due the delicious taste of it, cucumbers also contain some compounds that can be used as a drug, one of them is to prevent cancer. This plant contains Flavonoid, Saponin, and Tanin. These three compounds are the compounds which is in certain levels can be cytotoxic. Acute toxicity is an early screening test for a specifc cytotoxic potential crop for the development of anti-cancer drugs. The purpose of this research is to know the acute toxicity of cucumber (Cucumis sativus L) methanol extract to the Artemia salina Leach larvae by Brine Shrimp Lethality Test (BSLT) method. This research method was an experimental by using BSLT method. The test animal used was the Artemia salina Leach larvae. The method of extraction was maseration method. The results of the study can be seen from the percentage of larvae mortality for each concentration of extract, those are 0 μg / ml, 250 μg / ml, 500 μg / ml, 750 μg / ml, 1000 μg / ml are 0%, 48%, 62 %, 72%, 84% and 92%. Based on the result of probit analysis with microsoft Excel showed that LC50 value of cucumber methanol extract is 201,0165 μg /ml which means this extract have potency of acute toxicity because LC50 value <1000 μg / ml.Abstrak: Mentimun (Cucumis sativus L) adalah tanaman yang banyak dikonsumsi oleh masyarakat. Selain rasanya yang enak, mentimun juga memiliki kandungan senyawa yang dapat bermanfaat sebagai obat, salah satunya adalah untuk mencegah terjadinya kanker. Tanaman ini mengandung Flavonoid, Saponin, dan Tanin. Ketiga senyawa ini merupakan senyawa yang dengan kadar tertentu dapat bersifat sitotoksik. Toksisitas akut merupakan skrining awal untuk menguji suatu tanaman tertentu yang memiliki potensi sitotoksik untuk pengembangan obat anti kanker. Tujuan penelitian ini untuk mengetahui toksisitas akut dari ekstrak metanol mentimun (Cucumis sativus L) terhadap larva Artemia salina Leach dengan metode Brine Shrimp Lethality Test (BSLT). Metode penelitian ini berbentuk eksperimental dengan menggunakan metode BSLT. Hewan uji yang digunakan adalah larva Artemia salina Leach. Metode ekstraksi yang digunakan adalah metode maserasi. Hasil penelitian dapat dilihat dari persentase kematian larva untuk setiap konsentrasi ekstrak, yaitu 0 µg/ml, 250 µg/ml, 500 µg/ml, 750 µg/ml, 1000 µg/ml secara berturut-turut adalah 0%, 48%, 62%, 72%, 84% dan 92%. Berdasarkan hasil analisis probit dengan microsoft Excel menunjukkan nilai LC50 ekstrak metanol mentimun adalah 201, 0165 µg/ml yang berarti ekstrak ini memiliki potensi toksisitas akut karena nilai LC50 < 1000 µg/ml.

scholarly journals UJI TOKSISITAS AKUT MINUMAN PROBIOTIK SIRSAK GUNUNG (Annona montana Macf.) DENGAN METODE BSLT (Brine Shrimp Lethality Test)

2020 ◽  
Vol 1 (5) ◽  
pp. 941-948
Author(s):  
Ambar Fidyasari ◽  
Sentot Joko Raharjo ◽  
Melani Setyowati
Keyword(s):  
Acute Toxicity ◽  
Traditional Medicine ◽  
Brine Shrimp ◽  
Experimental Methods ◽  
Test Method ◽  
Toxic Effects ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
A Value ◽  
Acute Toxic

Soursop fruit (Annona montana Macf.) is one of the plants can be used as as traditional medicine. This plant contains terpenoid and acetogenin which can cause toxicity. The fruit has a flavor that is tasteless so the innovation becomes probiotic drinks. This drink has been proven as an antioxidant, antibacterial, antihyperuricemia and antidiarrheal. The aim of this study was to know about acute toxicity of probiotic drink of soursop juice using brine shrimp lethality test method which will be indicated by LC50 value. This study used experimental methods conducted in the Laboratory of Farmakoknosi. There are several variations in concentration in this study, namely 10000 ppm, 20000 ppm, 30000 ppm, 40000 ppm, 50000 ppm, 60000 ppm, 70000 ppm, 80000 ppm and replication was done 3 times with total number of test animals used was 270. The results showed that probiotic drink of soursop juice can provide acute toxic effects on test animals with LC50 value of 29717,23 ppm. LC50 values ​​indicate that the mountain soursop probiotic drink is not potentially toxic because it has a value of >1000 ppm.

scholarly journals A Cytotoxic Activity of Tahongai (Kleinhovia hospita Linn.) Leaves Extracts Using Brine Shrimp Lethality Test

2019 ◽  
Vol 4 (3) ◽  
pp. 60
Author(s):  
Indah Solihah ◽  
Herlina Herlina ◽  
Riana Sari Puspita Rasyid ◽  
Tri Suciati ◽  
Khairunnisa Khairunnisa
Keyword(s):  
Statistical Analysis ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Ethanol Extracts

Tahongai (Kleinhovia hospita Linn.) leaves had been known contain alkaloids, flavonoids, saponins, steroids, and tannins. At Komering, South Sumatera tribe, tahongai leaves had been known to treat tumor, cancer, polyps, acne, and dysmenorrhea. The study of cytotoxic activity of tahongai bark and stem were done. This study aims to determine the cytotoxic activity of tahongai leaves extracts using BSLT method. Tahongai leaves were extracted using gradual maceration with n-hexane, ethyl acetate, and ethanol 96%. Each extract was tested cytotoxic activity towards Artemia salina L. larvae. The yield of n-hexane, ethyl acetate, and ethanol extracts are 2,686%, 7,033%, and 7,933% respectively. Ethanol extract of tahongai leaves had the best cytotoxic activity with lethality value 76,667% at 500ppm. Statistical analysis with two way ANOVA showed extract and concentration had a significant (p<0,05) effect on larvae lethality percentage.

scholarly journals UJI TOKSISITAS EKSTRAK ETANOL DAUN JAMBU BIJI AUSTRALIA (Psidium guajava L) DENGAN METODE BSLT (Brine Shrimp Lethality Test)

2021 ◽  
Vol 9 (1) ◽  
pp. 10-17
Author(s):  
Nofita Nofita ◽  
◽  
Ade Maria Ulfa ◽  
Miera Delima ◽  
◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Ultrasonic Method ◽  
Ethanol Extract ◽  
Psidium Guajava ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Test Method ◽  
Toxicity Tests ◽  
Brine Shrimp Lethality ◽  
Guava Leaves

ABSTRACTGuava is one of the plants that can traditionally be used for the treatment of diseases. Many kinds of guava, one of which is the Australian guava has the characteristics of roots, stems, leaves, dark red fruit. This study aims to determine the toxicity of the ethanol extract of Australian guava leaves (Psidium guajava L) using the BSLT (Brine Shrimp Lethality Test) method and determine the chemical content of Australian guava leaves (Psidium guajava L). The extract was made by the ultrasonic method using 96% ethanol solvent. Toxicity tests were carried out using 48-hour-old Artemia salina Leach shrimp larvae. The toxic effect of the extract was identified by the percentage of shrimp larvae mortality using probit analysis (LC50). From the research results, phytochemical content includes tannins, flavonoids, alkaloids, terpenoids and saponins, and flavonoid compounds have the highest content compared to the others. Research shows that the ethanol extract of Australian guava leaves is of a moderate category (LC50 441,977 ppm).Keywords :Australia guava leaves, BSLT, Artemia salina L, Ultrasonic

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