scholarly journals Hygienic-sanitary quality in milk and cheese sold clandestinely in the municipality of Sinop - Mato Grosso, Brazil

2020 ◽  
Vol 13 (5) ◽  
pp. 52
Author(s):  
R. H. R. Pena ◽  
F. Freitas ◽  
B. G. Castro
Keyword(s):  
Escherichia Coli ◽  
Great Majority ◽  
Raw Milk ◽  
Human Consumption ◽  
Biochemical Tests ◽  
Pathogenic Potential ◽  
Mato Grosso ◽  
Total Coliforms ◽  
Intestinal Origin ◽  
Thermotolerant Coliforms

Dairy products such as raw milk and artisanal cheeses are marketed in open markets and directly at home throughout Brazil, even with prohibitions in Brazilian legislation, leading in many cases to the production of food without hygienic conditions that can be configured as an important source transmission of pathogens and, consequently, a danger to public health. Escherichia coli is an agent of intestinal origin and, in addition to some strains possess pathogenic potential, its presence in the food indicates inadequate hygienic practices. This study aimed to evaluate the hygienic-sanitary quality of these products to determine if they are suitable for human consumption. Eight samples of milk and eight cheese sold clandestinely in the municipality of Sinop in the State of Mato Grosso were analyzed. All of them were submitted to the Most Likely Number Test (NMP) for the detection of Total and Thermotolerant Coliforms, isolation in Methylene Blue Eosin Agar (EMB) and identification of Escherichia coli by means of biochemical tests. Among the 8 milk samples, 62.5% (5/8) presented levels of Thermotolerant Coliform than allowed by the legislation and 87.5% (7/8) above the levels allowed for Total Coliforms. Among the 8 cheese samples, 50% (4/8) presented values higher than that allowed for Thermotolerant Coliforms and 87.5% (7/8) for Total Coliforms. Of the 80 colonies initially isolated from raw milk and cheese samples, 56.25% (45/80) presented Escherichia coli characteristics. The great majority of the analyzed samples demonstrate a low hygienic-sanitary quality being considered possible sources of pathogens to the consuming population.

Identification of bacterial hazards in the production of artisan fresh cheese in Cuba

2020 ◽  
Vol 87 (2) ◽  
pp. 263-265
Author(s):  
Ailin Martínez ◽  
Nivian Montes de Oca ◽  
Mabelín Armenteros ◽  
Odalys Uffo ◽  
Yamilka Riverón ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Salmonella Enterica ◽  
Biofilm Formation ◽  
Raw Milk ◽  
Foodborne Diseases ◽  
Pathogenic Potential ◽  
Good Manufacturing Practices ◽  
Fresh Cheese ◽  
Isolated Species

AbstractArtisan fresh cheese producing farms from six provinces of Cuba were studied to identify the presence of bacterial hazards and the results are presented in this research communication. The bacterial hazards identified in milk and cheese respectively were: Listeria spp. (9.5 and 18.9%), Bacillus cereus (23.2 and 24.2%), Escherichia coli O157 (12.6 and 13.7%), Salmonella spp. (10.5 and 17.9%), and Staphylococcus aureus (29.5 and 51.6%). Listeria monocytogenes was not detected. Nine Salmonella serotypes corresponding to Salmonella enterica subsp. enterica and Salmonella enterica subsp. arizonae were isolated, whereas Salmonella Anatum was present most often. Biofilm formation by the isolated species and enterotoxin production by S. aureus strains demonstrated the pathogenic potential of the identified bacterial hazards. Results proved the presence of bacterial hazards in the raw milk and cheeses analyzed, so that good manufacturing practices must be accomplished throughout the entire production process in order to avoid the occurrence of foodborne diseases in the population.

Prevalence and pathogenic potential of Escherichia coli isolates from raw milk and raw milk cheese in Egypt

2016 ◽  
Vol 221 ◽  
pp. 69-76 ◽  
Author(s):  
Rabee A. Ombarak ◽  
Atsushi Hinenoya ◽  
Sharda Prasad Awasthi ◽  
Atsushi Iguchi ◽  
Ayaka Shima ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Raw Milk ◽  
Pathogenic Potential ◽  
Raw Milk Cheese

scholarly journals Microbiological and parasitological analysis of sand samples of recreation of the municipality of Sinop-MT areas

2020 ◽  
Vol 13 (3) ◽  
pp. 71
Author(s):  
F. Freitas ◽  
B. P. Deecken ◽  
R. H. R. Pena ◽  
I. P. Tancredi ◽  
B. G. Castro
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Human Interaction ◽  
Total Coliforms ◽  
Daily Exposure ◽  
Helminth Eggs ◽  
Recreation Areas ◽  
Three Samples ◽  
Thermotolerant Coliforms

Recreational sands are important places for human interaction, despite the risk to public health as a result of being exposed to the accumulation of bacteria, fungi, viruses and parasites due to daily exposure to waste produced by users, human secretions and animal excrement. The objective of this study was to perform the microbiological and parasitic analysis of 16 sandboxes for recreation in the municipality of Sinop-MT. The evaluations included: enumeration of total and thermotolerant coliforms, identification of Escherichia coli and endoparasites research. Of the samples surveyed, 68,75% (11/16) presented contamination by total coliforms and 43,75% (7/16) presented contamination by thermotolerant coliforms. The presence of Escherichia coli was detected in seven (43,75%) of the samples, and no contamination was detected by helminth eggs, but three samples (18,75%) were contaminated by larvae of the filiform and rabditoid type. In this way it is evident that the recreation areas of Sinop municipality have contamination that exposes the population to risk of infections, being necessary a better monitoring and hygiene so that it has an improvement of quality of these areas.

scholarly journals Genes de virulencia en Aeromonas spp. (Aeromonadales: Aeromonadaceae) aisladas de Oreochromis spp. (Perciformes: Cichlidae) para consumo humano en México

2018 ◽  
Vol 66 (4) ◽  
Author(s):  
JESSICA LIZBETH Ortega Balleza ◽  
Alejandro Sánchez-Varela ◽  
Isabel C. Rodríguez-Luna ◽  
Xianwu Guo
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Geographical Area ◽  
Virulence Gene ◽  
Gastrointestinal Diseases ◽  
Human Consumption ◽  
Emerging Pathogens ◽  
Pathogenic Potential ◽  
Aeromonas Caviae ◽  
And Control

The genus Aeromonas are widely distributed in aquatic ecosystems are Gram-negative rods, oxidase-positive, and glucose-fermenting, considered emerging pathogens in humans. Aeromonas belongs to the fish microbiota, these microorganisms have a diversity of virulence factors responsible for a variety of infections in humans mainly gastrointestinal diseases. The presence of Aeromonas in products intended for consumption with high commercial demand such as tilapia generates sanitary concern due to the pathogenic potential of this bacteria. In this context, identification of virulence genes in strains of Aeromonas isolated in Oreochromis spp. intended for human consumption in Reynosa, Tamaulipas, Mexico is important due to the lack of molecular studies in this geographical area. In the present study the pathogenic potential of 15 strains of Aeromonas (A. veronii, A. hydrophila and A. schubertii) from Oreochromis spp. for human consumption were analyzed. Through PCR six virulence genes were analyzed (alt, ast, aerA, hlyA, gcat and stx1) and the strains used as control were: Aeromonas hydrophila subsp. hydrophila ATCC 7966, Aeromonas caviae 429865 INP, Escherichia coli O157: H7 and Escherichia coli K12. El 100 % (n = 15) of the strains harbored at least one virulence gene, aerA gene was detected in 86.66% of the analyzed strains, while ast and stx1 genes were not identified. Moreover, Aeromonas strains had associated genes in the same strain: aerA / gcat, alt / aerA, alt / aerA / gcat / hlyA and alt / aerA / gcat, of which aerA / gcat were observed mostly in A. veronii, while A. hydrophila had the highest associations. These findings indicate that the strains of Aeromonas isolated in Oreochromis spp. have the potential to cause human diseases, and therefore, this species used as food, could be a vehicle for infections caused by Aeromonas. It also allows to provide information on this emerging microorganism to effectively treat and control any epidemiological event caused by Aeromonas spp. in the future.

scholarly journals Molecular characterization of Escherichia coli isolated from milk samples with regard to virulence factors and antibiotic resistance

2021 ◽  
pp. 2410-2418
Author(s):  
Waleed Younis ◽  
Sabry Hassan ◽  
Hams M. A. Mohamed
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
16S Rrna ◽  
Virulence Factors ◽  
Pathogenic Bacteria ◽  
Human Life ◽  
Raw Milk ◽  
Biochemical Tests ◽  
E Coli ◽  
Milk Samples

Background and Aim: Raw milk is considered an essential source of nutrition during all stages of human life because it offers a valuable supply of protein and minerals. Importantly, milk is considered a good media for the growth and contamination of many pathogenic bacteria, especially food-borne pathogens such as Escherichia coli. Thus, the objective of this study was to characterize E. coli and detect its virulence factors and antibiotic resistance from raw milk samples. Materials and Methods: Raw milk samples (n=100) were collected from different localities in Qena, Egypt, and investigated for the presence of E. coli using different biochemical tests, IMViC tests, serotyping to detect somatic antigen type, and molecularly by polymerase chain reaction (PCR) tests. The presence of different virulence and antimicrobial genes (hly, eae, stx1, stx2, blaTEM, tetA(A), and tetB genes) in E. coli isolates was evaluated using PCR. Results: The results demonstrated that 10 out of 100 milk samples were contaminated with E. coli. Depending on serology, the isolates were classified as O114 (one isolate), O27 (two isolates), O111 (one isolate), O125 (two isolates), and untypeable (five isolates) E. coli. The sequencing of partially amplified 16S rRNA of the untypeable isolates resulted in one isolate, which was initially misidentified as untypeable E. coli but later proved as Enterobacter hormaechei. Moreover, antibacterial susceptibility analysis revealed that nearly all isolates were resistant to more than 3 families of antibiotics, particularly to β-lactams, clindamycin, and rifampin. PCR results demonstrated that all E. coli isolates showed an accurate amplicon for the blaTEM and tetA(A) genes, four isolates harbored eae gene, other four harbored tetB gene, and only one isolate exhibited a positive stx2 gene. Conclusion: Our study explored vital methods for identifying E. coli as a harmful pathogen of raw milk using 16S rRNA sequencing, phylogenetic analysis, and detection of virulence factors and antibiotic-resistant genes.

scholarly journals Groundwater quality and underground flow in southern Amazon, Brazil

2019 ◽  
Vol 10 (6) ◽  
pp. 206-217
Author(s):  
Josilena De Jesus Laureano ◽  
Caryne Ferreira Ramos ◽  
Daíse Da Silva Lopes ◽  
Lindolaine Machado de Sousa ◽  
Maria Cristina Nery do Nascimento Recktenvald ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Large Range ◽  
Domestic Sewage ◽  
Fecal Coliforms ◽  
Human Consumption ◽  
Adequate Treatment ◽  
Total Coliforms ◽  
Physical Chemical ◽  
Maximum Value ◽  
Underground Flow

The aim of this study was to evaluate the physical, chemical and microbiological groundwater parameters as well as the mapping of groundwater flow in an urban area of Presidente Médici county in Rondônia State, Southern Amazon. The water samples were collected in 11 wells in April and June of 2017, which corresponds to the dry season in the studied region. Groundwater was acidic, with a large range of nutrients concentration, low turbidity and presence of fecal coliforms in most sampled wells. The parameters that were in disagreement with Consolidation Ordinance No 05/2017/MS and CONAMA no 396/08 Resolution, were pH, turbidity, total coliforms, Escherichia coli, and nitrate. Regarding the microbiological parameter, most of the sampling sites were in disagreement with the established by the legislation that it recommends to be absent in 100mL, when it is destined for human consumption. Densities found were higher than 112,000UFC/100mL for total coliforms and higher than 56,000UFC/100mL for Escherichia coli. The pH ranged from 4.1 to 6.4, and the turbidity showed a maximum value of 6.7 uT. The nitrate concentrations were above the established level (10 mg.L-1) in April in 72.7%, and in June 50% of the wells, with a maximum of 48.5 mg.L-1. The study of the direction of the underground flow demonstrated the predominance of the flow towards the nearest river, as well as potential contributors of microbiological influences. The results indicate contamination of the groundwater by domestic sewage, being proved by the presence of coliforms and nitrate, being the water unfit for human consumption without adequate treatment.

scholarly journals DIARRHEAGENIC Escherichia coli IN RAW MILK, WATER, AND CATTLE FECES IN NON-TECHNIFIED DAIRY FARMS

2019 ◽  
Vol 20 ◽  
Author(s):  
Laryssa F. Ribeiro ◽  
Mayhara M. C. Barbosa ◽  
Fernanda R. Pinto ◽  
Leticia F. Lavezzo ◽  
Gabriel A. M. Rossi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Water Samples ◽  
Dairy Farms ◽  
Raw Milk ◽  
Human Consumption ◽  
E Coli ◽  
Diarrheagenic Escherichia Coli ◽  
Milk Samples ◽  
Cattle Feces ◽  
Eaec Strain

Abstract This study focused on detecting diarrheagenic Escherichia coli, enteropathogenic E. coli (EPEC), Shiga-toxin-producing E. coli (STEC), enterohemorrhagic E. coli (EHEC or STEC:EPEC), enterotoxigenic E. coli (ETEC), and enteroaggregative E. coli (EAEC) in raw milk, water, and cattle feces sampled from non-technified dairy farms located in the northeastern São Paulo State, Brazil. Thirty-six water samples were collected at different points, namely, water wells (8 samples), water intended for human consumption (8 samples), water from milking parlor (8 samples), and water intended for animal consumption (7 samples), headwaters (1 sample), rivers (3 samples), and reservoirs (1 sample). Three raw milk samples were taken directly from bulk tanks in each farm, totalizing 24 samples. Feces samples were collected using rectal swabs from 160 bovines (20 animals per farm). E. coli was detected in 128 feces samples (80%), 16 raw milk samples (66.67%), and 20 water samples (55.56%). STEC (26 samples, 16.25%), EPEC (10 samples, 6.25%), STEC: EPEC (5 samples, 3.13%), and STEC: ETEC (1 sample, 0.63%) were the most prevalent strains detected in samples from cattle feces. EPEC, STEC, and STEC: EPEC strains were detected in 4.17% (1 sample), 16.67% (4 samples), and 4.17% (1 sample) of raw milk samples, respectively. STEC strains were detected in water used in the milking parlor, while no EAEC strain was detected. As a conclusion, cattle feces are important contamination sources of pathogenic E. coli in non-technified dairy farms and, consequently, cross-contamination among feces, water, and/or raw milk can occur. The use of quality water and hygienic practices during milking are recommended to avoid contamination since pathogens can be transmitted to humans via raw milk or raw milk cheese ingestion.

scholarly journals Temporal variation of faecal shedding of Escherichia coli O157:H7 in a dairy herd producing raw milk for direct human consumption

2014 ◽  
Vol 3 (3) ◽  
Author(s):  
Giuseppe Merialdi ◽  
Lia Bardasi ◽  
Laura Stancampiano ◽  
Roberta Taddei ◽  
Mauro Delogu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Temporal Variation ◽  
Dairy Herd ◽  
Raw Milk ◽  
Human Consumption ◽  
Escherichia Coli O157

scholarly journals MOLECULAR DETECTION AND CHARACTERIZATION OF ESCHERICHIA COLI ISOLATED FROM RAW MILK SOLD IN DIFFERENT MARKETS OF BANGLADESH

2017 ◽  
Vol 14 (2) ◽  
pp. 271-275 ◽  
Author(s):  
M. A. Islam ◽  
S. M. L. Kabir ◽  
S. K. Seel
Keyword(s):  
Escherichia Coli ◽  
Molecular Detection ◽  
Culture Media ◽  
Raw Milk ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
E Coli ◽  
Milk Samples ◽  
Antimicrobial Sensitivity

     The study was intended for molecular detection of E. coli isolated from raw cow’s milk. A total of 20 milk samples were collected from different upazila markets of Jamalpur, Tangail, Kishoreganj and Netrokona districts of Bangladesh. Milk samples were cultured onto various culture media for the isolation of bacteria. The isolated bacteria were identified by studying staining characteristics, cultural properties on different selective media, biochemical tests, catalase and coagulase test, and finally by PCR. Out of 20 samples, 15 (75%) milk samples were found positive for E. coli. 15 Escherichia coli isolates were amplified by 16S rRNA gene based PCR. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the E. coli isolates were resistant to amoxycillin (86.67%) and erythromycin (73.33%) but sensitive to azithromycin (53.33%), ciprofloxacin (86.67%), gentamicin (86.67%), norfloxacin (80%) and streptomycin (66.67%).

Sign in / Sign up

Export Citation Format

Share Document