scholarly journals Mycotic zoonoses risk from clipping instruments in Pet Shops in Sinop – MT

2021 ◽  
Vol 14 (10) ◽  
Author(s):  
Rayssa Tuanna Schmidt Balbinot ◽  
Dâmaris Carolini Teodoro Alves Costa ◽  
Neocimar Saraiva Correia ◽  
Fabiana Cristina Donofrio
Keyword(s):  
Filamentous Fungi ◽  
Microsporum Canis ◽  
Candida Spp ◽  
Malassezia Pachydermatis ◽  
Pathogenic Potential ◽  
Daily Lives ◽  
Sabouraud Agar ◽  
Urease Test ◽  
Close Relationship ◽  
The City

Pets have conquered the daily lives of families worldwide and because they have a close relationship with humans, they can transmit mycotic zoonoses such as dermatophytosis, malassezioses and candidosis. Studies show that fungi with pathogenic potential have already been granted in clipping instruments and bath articles in veterinary clinics and in Pet Shops. In the absence of data in the city of Sinop - MT, this study aimed to isolate fungi with pathogenic potential in clipping instruments used in the routine of Pet Shops and to identify etiological agents capable of causing mycotic zoonoses. Samples were carried out in 18 clipping instruments, without cleaning, from 10 Pet Shops (A, B, C, D, E, F, G, H, I, J) in the city of Sinop - MT with swabs and sterile carpets square and then seeded in Sabouraud agar with 0.05% chloramphenicol and mycobiotic agar, incubated at room temperature (25°C) for 30 - 45 days. The dermatophytes and non-dermatophyte filamentous fungi were identified using the microculture technique and urease test and as yeasts using a urease test, zymogram, auxanogram, germ tube and corn meal agar with polysorbate 80. Of the samples from 18 clipping instruments, 15 were positive for at least one fungal genus. The yeast that showed a high prevalence of isolation in clipping instruments from the studied Pet Shops (B, C, D, E, H and J) was Malassezia pachydermatis (86.7%), followed by the genus Candida spp. (C and D; 26.7%), filamentous fungi of the genus Aspergillus spp. (A; 13.3%), Microsporum canis (B; 13.3%) and Trichosporon spp. (J, 6.7%). Our results demonstrate that Pet Shops treat a large number of animals daily in a single environment, which may amplify the risk of spreading zoonoses, as an asymptomatic or sick carrier animal can potentially transmit the microorganism to other animals inside the store and thus to a large number of new pet owners. There is a need to reinforce the commercial requirements specialized in bathing and grooming in the city of Sinop - MT on the good practices of cleaning and disinfection of the elements used and the environment, eliminating or eliminating the risk of contracting mycotic zoonoses.

scholarly journals Rapid and real-time identification of fungi up to species level with long amplicon Nanopore sequencing from clinical samples

2020 ◽  
Author(s):  
Sara D’Andreano ◽  
Anna Cuscó ◽  
Olga Francino
Keyword(s):  
Real Time ◽  
Clinical Signs ◽  
Amplicon Sequencing ◽  
Species Level ◽  
Clinical Samples ◽  
Microsporum Canis ◽  
Mock Community ◽  
Candida Spp ◽  
Taxonomic Assignment ◽  
Malassezia Pachydermatis

Abstract The availability of long-read technologies, like Oxford Nanopore Technologies, provides the opportunity to sequence longer fragments of the fungal ribosomal operon, up to 6 Kb (18S-ITS1-5.8S-ITS2-28S), and to improve the taxonomy assignment of the communities up to species level and in real-time. We assess the applicability for taxonomic assignment of amplicons targeting a 3.5 Kb region (V3 18S-ITS1-5.8S-ITS2-28S D2) and a 6 Kb region (V1 18S-ITS1-5.8S-ITS2-28S D12) with the What's in my pot (WIMP) classifier. We used the ZymoBIOMICSTM mock community and different microbiological fungal cultures as positive controls. Long amplicon sequencing correctly identified Saccharomyces cerevisiae and Cryptococcus neoformans from the mock community and Malassezia pachydermatis, Microsporum canis, and Aspergillus fumigatus from the microbiological cultures. Besides, we identified Rhodotorula graminis in a culture mislabeled as Candida spp. We applied the same approach to external otitis in dogs. Malassezia was the dominant fungal genus in dogs' ear skin, whereas M. pachydermatis was the main species in the healthy sample. Conversely, we identified a higher representation of M. globosa and M. sympodialis in otitis affected samples. We demonstrate the suitability of long ribosomal amplicons to characterize the fungal community of complex samples, either healthy or with clinical signs of infection.

“I can’t just post anything I want”: Self-management of South Korean sports stars on social media

2021 ◽  
pp. 101269022110141
Author(s):  
Eunhye Yoo
Keyword(s):  
Social Media ◽  
Self Management ◽  
Public And Private ◽  
Daily Lives ◽  
The Public ◽  
South Korean ◽  
Digital Labor ◽  
Close Relationship ◽  
Study Participants ◽  
Depth Interviews

This study explores the influence and sociocultural meaning of self-management of South Korean sports stars in the context of their social media activity. The study utilizes netnography to analyze social media posts to determine the meaning of sports stars’ self-management. Data were collected through in-depth interviews with study participants. Ten South Korean sports stars, who are active users of Instagram, were selected as the study participants. Photographs, videos, and stories from their accounts—around 1800 posts in total—were analyzed. The results indicated that the sports stars attempted to share their daily lives on social media to build a close relationship with the public. Moreover, they used their accounts to publicize their commercialized selves and to promote their sponsors. They uploaded only strictly composed and curated posts on their accounts as a form of self-censorship. Finally, it was determined that digital labor was used for self-management on social media, where there is no distinction between public and private territory. A sports star has become a self-living commercial today, and self-management is now a prerequisite for survival. Thus, self-management on social media has become a requirement for sports stars.

scholarly journals Evaluation of pathogenic fungi occurrence in traumatogenic structures of freshwater fish

2011 ◽  
Vol 44 (2) ◽  
pp. 182-185 ◽  
Author(s):  
Fabio Caetano Oliveira Leme ◽  
Marcos Mendes de Barros Negreiros ◽  
Fernando Akira Koga ◽  
Sandra de Moraes Gimenes Bosco ◽  
Eduardo Bagagli ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
Fungal Infections ◽  
Pathogenic Fungi ◽  
Dematiaceous Fungi ◽  
Candida Spp ◽  
Pathogenic Potential ◽  
Infection Route ◽  
5.8S Rdna ◽  
Dorsal Fin ◽  
Pimelodus Maculatus

INTRODUCTION: Fungal infections in human skin, such as sporotrichosis, can occur after fish induced trauma. This work aimed to identify fungi in freshwater fish that are pathogenic to humans. METHODS: Extraction of dental arches from Serrassalmus maculatus (piranha) and Hoplias malabaricus (wolf fish), stings from Pimelodus maculatus (mandis catfish), dorsal fin rays from Plagioscion spp. (corvina) and Tilapia spp., for culture in Mycosel agar. Some cultures were submitted to DNA extraction for molecular identification by sequencing ITS-5.8S rDNA. RESULTS: Cultures identified most yeast as Candida spp., while sequencing also permitted the identification of Phoma spp. and Yarrowia lipolytica. CONCLUSIONS: While the search for S. schenckii was negative, the presence of fungus of the genera Phoma and Candida revealed the pathogenic potential of this infection route. The genus Phoma is involved in certain forms of phaeohyphomycosis, a subcutaneous mycosis caused by dematiaceous fungi, with reports of infections in human organs and systems. Traumatizing structures of some freshwater fish present pathogenic fungi and this may be an important infection route that must be considered in some regions of Brazil, since there are a large number of a fisherman in constant contact with traumatogenic fish.

scholarly journals Candida spp. occurrence in oral cavities of breastfeeding infants and in their mothers' mouths and breasts

2003 ◽  
Vol 17 (2) ◽  
pp. 151-155 ◽  
Author(s):  
Maria Stella Amorim da Costa Zöllner ◽  
Antonio Olavo Cardoso Jorge
Keyword(s):  
Oral Cavity ◽  
Candida Species ◽  
Statistical Difference ◽  
Control Group ◽  
Biochemical Tests ◽  
Candida Spp ◽  
Lactating Women ◽  
Sabouraud Agar ◽  
Breastfed Infants ◽  
Yeast Strains

This study aimed to determine the occurrence of Candida spp. in the oral cavity of predominantly breastfed infants and in their mothers' mouths and breasts, as well as in the oral cavity of bottlefed infants and in non-lactating women. One hundred and sixty nine women and eighty-five milk-fed infants took part in this study and were divided into four groups: 1) infants predominantly on breastfeeding (n = 55) and their mothers (n = 55); 2) infants on bottlefeeding (n = 30); 3) non-lactating women on whom oral collections were performed (n = 80) and, 4) non-lactating women on whom breast collections were performed (n = 34). Oral and mammary swabs were cultured on Sabouraud agar dextrose with chloramphenicol. The Candida yeast strains found were isolated and identified through morphological and biochemical tests. Candida species were much less frequent in infants who were predominantly breastfed than in those who were bottlefed. Yeasts were much more frequent on the breasts of lactating women, with statistical difference in relation to the control group.

scholarly journals EFICÁCIA DE SOLUÇÕES DESINFETANTES NA ELIMINAÇÃO DE FUNGOS DE IMPORTÂNCIA MÉDICA E VETERINÁRIA

2013 ◽  
Vol 18 (1) ◽  
Author(s):  
Isabel Martins Madrid ◽  
Alessandra Jacomelli Teles ◽  
Rosema Santin ◽  
Antonella Souza Mattei ◽  
Angelita Gomes ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Clinical Laboratory ◽  
Sporothrix Schenckii ◽  
Candida Spp ◽  
Malassezia Pachydermatis ◽  
Clinical Laboratory Standard Institute ◽  
Clinical Laboratory Standard

Programas de higiene e desinfecção são essenciais no controle ambiental de agentes fúngicos potencialmente patogênicos aos homens e animais, dessa forma objetivou-se avaliar a ação antifúngica do hipoclorito de sódio 4% e digluconato de clorexidina 6,6% em doze isolados fúngicos. Foram testados fungos isolados do ambiente e de casos clínicos como Aspergillus spp, Candida spp, Microsporum spp, Malassezia pachydermatis, Cryptococcus neoformans e Sporothrix schenckii. Foram utilizadas as técnicas de microdiluição em caldo e difusão em ágar conforme protocolos descritos pelo Clinical Laboratory Standard Institute (CLSI) com adaptações para agentes químicos. Todos os isolados foram sensíveis a concentrações inferiores a recomendada pelo fabricante do digluconato de clorexidina (6,6%) com CIM e CFM entre ≤0,42 a 1,68%. Para o hipoclorito de sódio, 58,3% dos isolados foram resistentes a concentração recomendada pelo fabricante (4%) apresentando CIM e CFM entre 2 e >8%. O digluconato de clorexidina apresentou maiores zonas de inibição do que o hipoclorito de sódio, com halos de até 31mm de diâmetro. A clorexidina demonstrou ação fungicida em baixas concentrações sendo eficaz na eliminação de fungos filamentosos e leveduriformes de importância médica e veterinária, enquanto o hipoclorito de sódio somente obteve esta ação em altas concentrações.

scholarly journals Plans d’eau glaciaires et isostasie dans les bassins de la haute Ware et de la rivière Otter, Massachusetts (2e partie)

2005 ◽  
Vol 17 (41) ◽  
pp. 251-264
Author(s):  
Gilbert Cestre
Keyword(s):  
New England ◽  
Eastern Canada ◽  
Low Area ◽  
Proglacial Lakes ◽  
Close Relationship ◽  
Long Time ◽  
History Of ◽  
Connecticut Valley ◽  
The City ◽  
Detailed Work

The present study is one of the unpublished research projects which are known to have been conducted in New England and in Eastern Canada under the guidance of the late Richard J. LOUGEE, long-time professor of Geomorphology at Clark University. Over a number of years, this writer has worked in close relationship with Lougee and much evidence in the field was studied together. It is believed that here has been recorded a most detailed work of surveying, and this undoubtedly accounts for the somewhat exceptional results that will be presented. The area selected for this study (about 80% of it is woodland) is located in the highlands of Central Massachusetts in Worcester County, about twenty miles (32 kilo-meters) northwest of the city of Worcester. It consists of the valley of the Otter River draining north, and of a small portion of the East Branch of the Ware River draining south. Since completion of this study, parts of the low area which held the ancient glacial lakes have been flooded to become water reservoirs. That proglacial lakes, though temporary they may have been, once submerged much of the area under study, is shown by an abundance of deltas, kames, eskers and deltaic kames terraces. It is believed that all of these were built under water in such lakes. Other features, such as kettle-holes and glacial outlets, especially ice-marginal channels cut diagonally down the slope, have also been studied. By plotting on a profile of the most characteristic elevations (often carefully surveyed), it is possible to find the water planes of ancient proglacial lakes. To this must be added experiments conducted in a sedimentation tank as also measurements of both the imbrication of cobbles in eskers and the « smoothness indexes » of such stones and pebbles, using A. Cailleux' methods. Thus were obtained results which tend to show that : 1- the area under study probably was in a deep interlobate space created between the Connecticut Valley lobe to the west and the Boston Basin lobe to the east ; 2— ice-marginal channels are an indication of the existence of a thick, fast-retreating ice border ; 3- an isostatic balance restored itself by sometimes quick and strong adjustments of the crust of the earth ; 4— an early upwarping, made up of various zones of tilting articulated on hinge lines, has been referred to as Hubbard Uplift and is the earliest known in the post-Glacial history of New England.

scholarly journals Social Tourism 'In Practice': The Tourist Experience of Homeless People in the Metropolitan Region of Rio de Janeiro-RJ, Brazil

2020 ◽  
Vol 12 (4) ◽  
pp. 1017-1038
Author(s):  
Jordania de Oliveira Eugenio ◽  
◽  
Bernardo Lazary Cheibub ◽  
Keyword(s):  
Participant Observation ◽  
Homeless People ◽  
Metropolitan Region ◽  
Daily Lives ◽  
Tourist Attractions ◽  
Tourist Experience ◽  
Prejudice And Discrimination ◽  
The Right ◽  
The City ◽  
Extension Program

When they are disregarded as citizens, people on the street seem to be invisible with regard to their rights, while in their daily lives their presence causes discomfort, generating the inverse of invisibility. This work, when undertaking tourism as a social right, describes how the tourist experiences of a group of homeless people - assisted by a public social assistance agency, in partnership with a UFF extension program - took place, which they visited tourist attractions in the metropolitan region of Rio. In addition to contact with authors / research that dealt with the reality of people living on the streets, the theoretical basis intertwined the themes of the Right to Leisure and the City, the Tourist Experience and Social Tourism. The analyzes carried out from ethnographic bases, including participant observation and interviews with a semi-structured script, indicated that tourist experiences seem to have aroused affective memories that work for this group as a means of resistance to the condition in which they find themselves. Even so, some experiences were crossed by serious situations of prejudice and discrimination, directed by conventional visitors to the group.

scholarly journals Monitoring of Dermatophytosis incidence in domestic dogs and cats in Kharkiv, Ukraine

2019 ◽  
Vol 5 (1) ◽  
pp. 17-19
Author(s):  
G. V. Ponomarenko ◽  
V. L. Kovalenko ◽  
O. V. Ponomarenko ◽  
R. V. Severyn ◽  
A. M. Gontar ◽  
...  
Keyword(s):  
Laboratory Tests ◽  
Trichophyton Mentagrophytes ◽  
Domestic Dogs ◽  
Microsporum Canis ◽  
Diagnostic Studies ◽  
High Level ◽  
Mold And Yeast ◽  
The City ◽  
Mycological Laboratory

The spread of dermatophytosis among animals and humans in the localities of Ukraine in particular in the city of Kharkiv is due to the presence of range of the most susceptible animals. This information mainly concerns dogs and cats that can be affected by dermatophytosis and may be a reservoir of dermatophyte fungus. Particularly important is that domestic dogs and cats represent a significant epidemiological threat to the population. The purpose of the research was to monitor the morbidity of dermatophytosis in domestic dogs and cats in Kharkiv. Diagnostic studies of dermatophytosis morbidity level determination in domestic dogs and cats were performed complexly including the clinical and epizootic data, microscopic and mycological laboratory tests conducted according to generally accepted methods (Kovalenko et al., 2017; Sutton, Fothergill and Rinaldi, 2001). Dermatophytosis was diagnosed among 231 animals in the study of 1,277 domestic dogs kept by the inhabitants of the city of Kharkiv which was in 18.09% of samples. Dermatophytosis was also detected in 615 animals which constitutes 50.25% in the study of 1,124 cats. The cultures of dermatophytosis agent Microsporum canis were isolated from 126 dogs (18.98%) and 110 cats (40.74%). Trichophyton mentagrophytes cultures were isolated from 16 dogs (2.41%) and 16 cats (5.93%). The percentage of isolated cultures of mold and yeast-like fungi in the studies of sick dogs and cats were 38.55% and 22.25% respectively. Obtained results indicate quite high level of the dermatophytosis spreading among domestic dogs and cats in the city of Kharkiv.

scholarly journals Change of Meaning in Space and Form of Contemporary Karo Architecture

2019 ◽  
Vol 1 (4) ◽  
pp. 121-131
Author(s):  
Mohammed Nawawiy Loebis ◽  
Imam Faisal Pane ◽  
Wahyu Abdillah ◽  
Aurora S. Lubis
Keyword(s):  
Qualitative Methods ◽  
Architectural Heritage ◽  
Place Of Residence ◽  
Daily Lives ◽  
Fertile Soil ◽  
The Times ◽  
North Sumatra ◽  
The City

Karo Plateau is one of the most valuable assets for North Sumatra Province, Indonesian. In addition to fertile soil and producing good quality vegetables and fruit, this plain also has architectural heritage to be proud of. Several villages up to now can be seen and visited which stores various Karo architectural works including some traditional buildings and houses. Change with the times, the movement of people also occurs for various reasons. The development of the city in this province makes the villagers look for livelihood in the city. However, the inheritance of ancestors was still held firmly to the present with a different format. The Karo family currently lives in shophouse and is no longer in a traditional house and they have a business there. The perception of space in traditional houses really carried over in their daily lives. Using qualitative methods, this article explains the interpretation of the different perceptions of Karo people regarding the space and form of their place of residence. However, the essence or meaning that exists in the traditional Karo architecture is still approved until now in a different form and format.

ROSEOFUNGIN-AS, OINTMENT 2% FOR TREATMENT OF TINEA PEDIS AND TINEA CORPORIS: RESULTS OF A RANDOMIZED, BLINDED, PROSPECTIVE, MULTICENTER PHASE III CLINICAL TRIAL

2021 ◽  
pp. 29-39
Author(s):  
А.К. САДАНОВ ◽  
В.Э. БЕРЕЗИН ◽  
И.Р. КУЛМАГАМБЕТОВ ◽  
Л.П. ТРЕНОЖНИКОВА ◽  
А.С. БАЛГИМБАЕВА
Keyword(s):  
Clinical Trial ◽  
Candida Albicans ◽  
Tinea Pedis ◽  
Fungal Infections ◽  
Tinea Corporis ◽  
Phase Iii ◽  
Microsporum Canis ◽  
Candida Spp ◽  
Reference Drug ◽  
Phase Iii Clinical Trial

Розеофунгин-АС, мазь 2% для наружного применения разработана для лечения микозов кожи разной этиологии, вызванных дерматофитными, дрожжеподобными и плесневыми грибами. Многоцентровое слепое проспективное рандомизированное исследование проводили в Республике Казахстан для сравнения терапевтической эффективности и безопасности препаратов «Розеофунгин-АС, мазь 2%» и «Клотримазол, крем 1%» при лечении tinea pedis и tinea corporis. Препараты применяли 2 раза в день в течение 28 дней. Лабораторные общеклинические и биохимические обследования пациентов проводили на 0, 14 и 28 дни исследования. Микологическое обследование включало микроскопические и культуральные исследования, которые проводились до лечения и на 28-30-й день после окончания лечения. В клиническое исследование III фазы входили 410 пациентов, включая 290 пациентов с tinea pedis и 120 пациентов с tinea corporis. Лечение препаратом «Розеофунгин-АС, мазь 2%» получали 310 пациентов, лечение препаратом «Клотримазол, крем 1%» - 100 пациентов. У больных обеих групп уже на 14 день уменьшалась выраженность субъективных и объективных проявлений. К 28 дню практически все клинические признаки патологического процесса отсутствовали. Совокупная клинико-микологическая эффективность препарата «Розеофунгин-АС, мазь 2%» составила 99,1%, препарата «Клотримазол, крем 1%» - 98,0%. Препарат «Розеофунгин-АС, мазь 2%» проявил высокую эффективность при лечении грибковых инфекций tinea pedis и tinea corporis, вызванных возбудителями трихофитии (Trihophyton rubrum, T. violarum, T. tonsurans), микроспории (Microsporum canis, M. gypseum), кандидоза (Candida albicans, Candida spp.), плесневыми грибами (Penicillium glaucum). В исследовании не были зафиксированы аллергические реакции и индивидуальная непереносимость исследуемого и референтного препаратов. Препарат «Розеофунгин-АС, мазь 2%» является эффективным антимикотическим средством, клинико-микологическая эффективность которого в терапии микозов кожи составляет 99,1%. Препарат имеет высокий уровень переносимости, безопасности и приемлемости RoseofunginAS, ointment 2% for external use was developed for the treatment of skin mycoses of various etiologies caused by dermatophytic and yeastlike fungi and molds. A multicenter, blind, prospective, randomized trial was conducted in the Republic of Kazakhstan to compare the therapeutic effectiveness and safety of RoseofunginAS, ointment 2% and Clotrimazole, cream 1% in the treatment of tinea pedis and tinea corporis. The drugs were administered twice daily for 28 days. Laboratory general clinical and biochemical examinations of patients were performed on days 0, 14, and 28 of the trial. Mycological examination included microscopic and cultural studies, which were carried out before treatment and on days 2830 after the end of treatment. The Phase III clinical trial involved 410 patients, including 290 with tinea pedis and 120 with tinea corporis. Treatment with RoseofunginAS, ointment 2% was administered to 310 patients, 100 patients were treated with Clotrimazole, cream 1%. In patients of both groups, the severity of subjective and objective manifestations decreased already on day 14. By day 28, almost all clinical signs of the pathological process were absent. The cumulative clinical and mycological effectiveness of the drug RoseofunginAS, ointment 2% was 99.1% and that of the drug Clotrimazole, cream 1% reached 98.0%. The drug RoseofunginAS, ointment 2% exhibited high effectiveness in the treatment of fungal infections, including tinea pedis and tinea corporis, with pathogens that cause trichophytosis (Trihophyton rubrum, T. violarum, T. tonsurans), microsporia (Microsporum canis, M. gypseum), candidiasis (Candida albicans, Candida spp.), and molds (Penicillium glaucum). The study did not record allergic reactions and individual intolerance to the study and reference drug. The drug RoseofunginAS, ointment 2% is an effective antimycotic agent, the clinical and mycological effectiveness of which in the treatment of skin mycoses was 99.1%. The drug possesses a high level of tolerance, safety and acceptability.

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