scholarly journals CHEMICAL EVIDENCE SUPPORTING THE ICLUSION OF AMARANTHACEAE AND CHENOPODIACEAE INTO ONE FAMILY AMARANTHACEAE JUSS. (s.l.)

2020 ◽  
pp. 271-278
Author(s):  
Fatima Mubark ◽  
Ikram Madani Ahmed
Keyword(s):  
Octadecadienoic Acid ◽  
Morphological Diversity ◽  
Chemical Compounds ◽  
Mass Analysis ◽  
Closely Related Species ◽  
Methanolic Extracts ◽  
Chenopodium Murale ◽  
Close Relationship ◽  
Gomphrena Celosioides ◽  
Layer Chromatography

In this study, separation of chemical compounds using Thin layer chromatography technique revealed close relationship between the studied members of the newly constructed family Amaranthaceae Juss. (s.l.). 68% of the calculated affinities between the studied species are above 50% which is an indication for close relationships. 90% is the chemical affinities reported between Chenopodium murale and three species of the genus Amaranthus despite of their great morphological diversity. Among the selected members of the chenopodiaceae, Chenopodium murale and Suaeda monoica are the most closely related species to all of the studied Amaranthaceae . 60%-88% and 54%-88% chemical affinities were reported for the two species with the Amaranthaceae members respectively. GC-Mass analysis of methanolic extracts of the studied species identified 20 compounds common between different species. 9,12-Octadecadienoic acid (Z,Z)-,2-hydroxy-1 and 7-Hexadecenal,(Z)- are the major components common between Amaranthus graecizans, Digera muricata Aerva javanica Gomphrena celosioides of the historical family Amaranthaceae and Suaeda monoica Salsola vermiculata Chenopodium murale Cornulaca monocantha of the historical family Chenopodiaceae, Most of the identified compounds are of pharmaceutical importance such as antioxidants, anti-inflammatory , and Anti-cancerous. KEYWORDS: Chemical affinity; TLC; GC- Mass analysis; Amarancaceae; Chenopodiaceae

scholarly journals Physiologically Active Compounds in Four Species of Phellinus

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Katarzyna Sułkowska-Ziaja ◽  
Anna Maślanka ◽  
Agnieszka Szewczyk ◽  
Bożena Muszyńska
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Total Content ◽  
Dry Weight ◽  
Hplc Method ◽  
Indole Compounds ◽  
Methanolic Extracts ◽  
Free Phenolic Acids ◽  
Layer Chromatography ◽  
Physiologically Active Compounds

The content of two groups of compounds with biological activity (non-hallucinogenic indole compounds and free phenolic acids) were analyzed in extracts of fruiting bodies of four species of Phellinus: P. igniarius, P. pini, P. pomaceus and P. robustus. The presence of indole compounds in methanolic extracts was analyzed by high-performance liquid chromatography and thin-layer chromatography coupled with densitometric detection. Three metabolites (serotonin, tryptamine, and L-tryptophan) were identified. The contents of individual indole compounds ranged from 1.70 (tryptamine in P. robustus) to 8.32 mg x 100 g1 dry weight (L-tryptophan in P. robustus). Four free phenolic acids were detected in methanolic extracts by the HPLC method. The total content ranged from 9.9 mg x 100 g1 DW (P. igniarius) to 32.5 mg x 100 g1 DW (P. robustus).

Primary Phytochemical Evaluation of P. oleracea and P. quadrifida

2021 ◽  
pp. 3789-3793
Author(s):  
T. P. Dugawale ◽  
C.C. Khanwelkar ◽  
P.P. Durgawale
Keyword(s):  
Quality Assurance ◽  
Extraction Techniques ◽  
Quality Assurance Procedure ◽  
Closely Related Species ◽  
Recent Interest ◽  
Percentage Yield ◽  
Crude Drugs ◽  
Primary Evaluation ◽  
Layer Chromatography

P. oleracea and P. quadrifida are closely related species belonging to family Portulaceae. They are found in almost parts of the globe growing as neglected weed. However, recent interest in these plants owing to their palatable sour taste and health benefits have made them acceptable as cultivable plants and potted herbs. Even though numerous studies indicate the presence of pharmacological compounds in these plants, reports on quality assurance practices followed for evaluating these plants as crude drugs have not been detailed. In this study, the plant species were harvested as weed, authenticated, extracted using different solvents and extraction techniques. The extracts were then evaluated and studied for their primary phytochemical composition. This was followed by isolation of beta sitosterol using thin layer chromatography and column chromatography. The results indicate the presence all major classes of phytochemicals such as steroids, flavanoids, alkaloids, saponins, polysaccharides, and tannins. The percentage yield, extractive values, ash values, moisture content were calculated for both the species. The isolation of beta-sitosterol may be used as a quality assurance procedure. The data presented in this study may be further used by researchers for primary evaluation of quality of crude drugs in the form of P. oleracea and P. quadrifida plants.

scholarly journals TLC-based fingerprinting for Phyllanthus niruri from diverse geographical origins in East and Central Java Indonesia

2021 ◽  
Vol 948 (1) ◽  
pp. 012003
Author(s):  
K Kartini ◽  
W A Wulandari ◽  
N I E Jayani ◽  
F Setiawan
Keyword(s):  
Geographical Location ◽  
Chemical Compounds ◽  
Harvest Time ◽  
Chemical Similarity ◽  
Phyllanthus Niruri ◽  
Central Java ◽  
Tropical Areas ◽  
Crude Drugs ◽  
Layer Chromatography

Abstract Phyllanthus niruri L. (meniran), the member of Euphorbiaceae, is a medicinal plant that is commonly found in tropical and sub-tropical areas such as Asia, America, and China. Various factors such as environment, geographical location, harvest time, and post-harvest process can affect the quality of crude drugs produced from P. niruri. The objective of this study was to evaluate the quality of meniran herbs obtained from 15 geographical origins in East and Central Java, Indonesia using Thin Layer Chromatography (TLC) profiles analyzed by chemometrics. TLC was carried out using TLC plate Si Gel 60 GF254 as stationary phase; toluene, ethyl acetate, methanol, 85% formic acid (75:25:25:6) as mobile phase; and visualized using NP/PEG Reagent. The results showed TLC-fingerprinting combined with chemometric (PCA and CA) analyses were able to discriminate the origin of P. niruri from different geographical origins. P. niruri from 15 locations of East and Central Java Indonesia were classified into 5 groups based on their chemical similarity. The samples that are grouped in one cluster have the similar quality of chemical compounds, while the samples in different clusters also have different qualities.

scholarly journals Pharmacognostic Study of Sangkareho Root (Callicarpa longifolia Lam.) from Pelaihari, South Kalimantan

2020 ◽  
Vol 3 (2) ◽  
pp. 58-63
Author(s):  
Fitriyanti Fitriyanti ◽  
Yusmalina Yusmalina ◽  
Rahmi Muthia
Keyword(s):  
Chemical Compounds ◽  
Scientific Basis ◽  
Test Results ◽  
Calcium Oxalate Crystals ◽  
Plant Parts ◽  
Eluent Ethyl Acetate ◽  
Pungent Odor ◽  
Layer Chromatography ◽  
Positive Results ◽  
Light Brown Color

Sangkareho (Callicarpa longifolia Lam.) is used traditionally by one of Kalimantan's indigenous tribes, the Dayak Tunjung tribe as a medicine for colds and inflammation, where the plant parts used are the roots. Considering its very potential prospects, research aimed at providing a scientific basis for plant pharmacognostic data needs to be carried out with qualitative methods. The qualitative examination is done by several methods including test identification of organoleptic, macroscopic, microscopic, and chemical compounds. Organoleptic test results showed that the roots have a light brown color, bitter and slightly spicy, and a rather pungent odor. Microscopic test results showed sangkareho root has a length of � 90 cm; width of � 1 cm; and for the form of a spear with a ride root system. Microscopic observations are found in the form of epidermal cells, exodermis, cortex, endodermis, bearing files, calcium oxalate crystals, and stone cells. The identification of chemical compounds showed positive results against alkaloids, flavonoids, saponins, and triterpenoids. The thin-layer chromatography profile shows four separate stains with eluent ethyl acetate : methanol : water in a ratio of 8 : 2 : 1, respectively.

scholarly journals Assessment of camel thorn (Alhagi maurorum) as new sources of bioactive compounds using GC-MS technique

Plant Omics ◽  
2019 ◽  
pp. 70-77
Author(s):  
Reham M. Mostafa ◽  
Heba S. Essawy
Keyword(s):  
Petroleum Ether ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Petroleum Ether Extract ◽  
Chemical Compounds ◽  
Mass Analysis ◽  
Hexadecanoic Acid ◽  
Anticancer Compounds ◽  
The Family

Alhagi maurorum (A. maurorum) is one of the medicinally important plants belonging to the family leguminasae, commonly known as camel thorn. This research was amid to identify the chemical compounds in the aerial part of A. maurorum using GC-mass analysis. Three solvents with different polarities were used for the extraction of chemical constituents (water, methanol and petroleum ether). The results of GC-MS analysis led to identification of various compounds. In total, thirty-nine compounds from petroleum ether extract, thirty-two compounds in methanolic extract and seventeen compounds in aqueous extract were identified. Majority of the identified compounds have been reported to possess many biological activities. Among them, we reported 10 new anticancer compounds (Vitamin E; Hexadecanoic acid; Stigmast-5-en-3-ol; Phytol,2-hexadecen-1-ol,3,7,11,15-tetramethyl; Squalene; Hexadecanoic acid; 2-hydroxy-1-(hydroxymethyl) ethyl ester; Oxime,methoxy-phenyl,methyl N-hydroxyben-zenecarboximidoate; Ergost-5-en-3-ol; 9,12- Octadecad-ienoic acid and Farnesol) from A. maurorum using three solvent, while the best effective solvent was petroluem ether. Therefore, we report that A. maurorum has great potential to be developed into anticancer drugs.

scholarly journals DIFFERENTIATION OF Curcuma longa, Curcuma xanthorrhiza and Zingiber cassumunar BY THIN LAYER CHROMATOGRAPHY FINGERPRINT ANALYSIS

2011 ◽  
Vol 11 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Mohamad Rafi ◽  
Eti Rohaeti ◽  
Ali Miftahudin ◽  
Latifah K. Darusman
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Curcuma Longa ◽  
Chemical Compounds ◽  
Specific Marker ◽  
Fingerprint Analysis ◽  
Fingerprint Pattern ◽  
Curcuma Xanthorrhiza ◽  
Zingiber Cassumunar ◽  
Layer Chromatography

Turmeric (Curcuma longa), java turmeric (Curcuma xanthorrhiza) and cassumunar ginger (Zingiber cassumunar) are widely used in traditional Indonesian medicine. These three herbs have relatively similar rhizomes colour so it is difficult to be differentiated especially if they are in powder form. A rapid and reliable method, thin layer chromatography (TLC) fingerprint, has been developed in order to identify, authenticate and differentiate these three herbs through fingerprint profile of chemical compounds. TLC fingerprints of the three herbs were obtained by visualization of separate zones with visible and UV (254 and 366 nm) light. The TLC fingerprint pattern is different each other and showed a specific marker zones respectively. Therefore, TLC fingerprint can be utilized for identification, authentication and differentiation method in quality control of the three herbs tested.

scholarly journals HPTLC METHOD FOR ESTIMATION AND QUANTIFICATION OF β-SITOSTEROL FROM IN-VIVO AND IN-VITRO SAMPLES OF MERREMIA AEGYPTIA AND MERREMIA DISSECTA

2020 ◽  
pp. 162-165
Author(s):  
RIDHI JOSHI ◽  
RISHIKESH MEENA ◽  
PREETI MISHRA ◽  
VIDYA PATNI
Keyword(s):  
High Performance ◽  
Normal Phase ◽  
Leaf Sample ◽  
Plant Parts ◽  
Methanolic Extracts ◽  
Aluminum Plates ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: A normal-phase high-performance thin-layer chromatography (HPTLC) method has been developed and validated for estimation and quantitation of beta-sitosterol from the methanolic fraction of different plant parts of two medicinally important plants viz. Merremia aegyptia and Merremia dissecta. These plants have been reported to possess antimicrobial, antioxidant, and anti-inflammatory activities. Methods: Chromatographic separation of beta-sitosterol from the methanolic extracts of plant parts of M. aegyptia and M. dissecta was performed on TLC aluminum plates pre-coated with silica gel 60F254 using a suitable mobile phase. The densitometric scanning was done after derivatization at ????-580 nm for ????-sitosterol. Result: Only M. dissecta leaf sample was reported to contain ????-sitosterol (4.6 ng/μl), whereas other samples such as seed, stem, and callus extracts of M. aegyptia and M. dissecta did not showed its presence. Conclusion: The developed HPTLC method is simple, rapid, and precise and can be used for routine analysis and quantification of ????-sitosterol and other useful plant bioactives that are phytopharmaceutically important.

scholarly journals The phytochemical screenings and thin layer chromatography analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.)

2005 ◽  
Vol 3 (1) ◽  
pp. 26-31 ◽  
Author(s):  
SOERYA DEWI MARLIANA ◽  
VENTY SURYANTI ◽  
SUYONO SUYONO
Keyword(s):  
Thin Layer ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Soxhlet Extraction ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Thin Layer Chromatography Analysis ◽  
Chromatography Analysis ◽  
Sechium Edule ◽  
Layer Chromatography

The phytochemical screenings and analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.) with Thin Layer Chromatography (TLC) has been carried out. Isolation was done by Soxhlet extraction for 6 hours with petroleum ether and the residue was extracted by maceration during 24 hours with ethanol.The isolated compounds in ethanol extract were identified by phytochemical screenings method and TLC. The result showed the presence of alkaloid, saponin, cardenolin/bufadienol and flavonoid.

Buellia tesserata and dimelaena radiata, two closely related species

2003 ◽  
Vol 35 (2) ◽  
pp. 117-124 ◽  
Author(s):  
Victor J. Rico ◽  
Vicent Calatayud ◽  
Mireia Giralt
Keyword(s):  
Related Species ◽  
Closely Related Species ◽  
Close Relationship ◽  
Generic Position

AbstractThe morphology, anatomy, ecology and distribution of Buellia tesserata and Dimelaena radiata are discussed. New data on their chemistry are given. The present study reveals a very close relationship between both taxa and consequently their different generic position is questioned. Buellia fimbriata is reduced to synonymy with Buellia tesserata.

scholarly journals Reevaluation of Host Specificity of the Closely Related Species Pseudoperonospora humuli and P. cubensis

Plant Disease ◽  
2012 ◽  
Vol 96 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Fabian Runge ◽  
Marco Thines
Keyword(s):  
Closely Related Species ◽  
Bryonia Dioica ◽  
Virulence Potential ◽  
Pseudoperonospora Humuli ◽  
Host Jump ◽  
High Virulence ◽  
Close Relationship ◽  
Internal Transcribed Spacer Sequences ◽  
Relationship Of ◽  
Limited Possibility

Pseudoperonospora cubensis causes some of the most devastating diseases of cucurbitaceous crops, while P. humuli is an important pathogen of hop (Cannabaceae). Although parasitic to different Angiosperm orders, these pathogens are highly similar, both in morphology and based on molecular comparisons. Considering the close relationship of P. humuli and P. cubensis, it was hypothesized that cross infectivity of the pathogens between their optimum hosts might be possible. Two strains of P. humuli and one of P. cubensis used in this study were able to reproduce on the two wild cucurbit relatives Bryonia dioica and Sicyos angulatus. Interestingly, limited infectivity of the P. cubensis strain to hop was also observed, and the P. humuli strain was also able to infest Cucumis sativus. The cross infections were verified by comparing the nuclear ribosomal-internal transcribed spacer sequences of newly produced sporangiophores from the different hosts. Morphologically the two pathogens could be distinguished by statistical analyses on their original hosts but these differences diminished on some alternate hosts. The ability of P. cubensis to infest the perennial Bryonia dioica and Humulus lupulus might offer a limited possibility for overwintering in temperate regions and needs to be evaluated in future studies. The ability of P. humuli to complete its life cycle on all the tested cucurbits provides evidence that the host jump across orders may continue and points to the high virulence potential of this pathogen.

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