scholarly journals Uji Prebiotik Mangga Manalagi (Mangifera indica L. var manalagi) Terhadap Pertumbuhan Lactobacillus plantarum Secara In Vitro

2021 ◽  
Vol 6 (2) ◽  
pp. 102
Author(s):  
Iit Lusif Tsania ◽  
Irul Hidayati ◽  
Ita Ainun Jariyah
Keyword(s):  
Carbon Source ◽  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Short Chain Fatty Acid ◽  
Mangifera Indica ◽  
Food Sources ◽  
Beneficial Bacteria ◽  
Food Ingredients ◽  
Experimental Laboratory

<p><strong>Prebiotics are food ingredients that cannot be absorbed by the host and will be fermented by colonic bacteria into Short-Chain Fatty Acid. Prebiotics can come from various natural ingredients such as wheat, onions, and bananas. Prebiotic food sources can stimulate the growth of probiotic bacteria which are beneficial bacteria for the host, one of which can produce bacteriocins to fight pathogenic bacteria. Manalagi mango (Mangifera indica L. var manalagi) is a fruit that has the potential as a source of prebiotics because it contains prebiotic food requirements, including dietary fiber and carbohydrates. The purpose of this study is to find out the ability or potential of Manalagi mengkal mango as a source of prebiotics by using a prebiotic test based on the growth of BAL is Lactobacillus plantarum in vitro. The design of this study used an experimental laboratory carried out in vitro by making carbon source media with various concentrations of mango flour, then mixed with liquid MRS, and given Lactobacillus plantarum culture which then measured the carbon source medium in the form of TPC. The highest TPC average yield was 1,7 x 1010 CFU/ml is the variation in the concentration of mango flour 6.89%. Based on the results of a prebiotic test with TPC mango flour manalagi mengkal showed its ability as a source of prebiotics by stimulating the growth of BAL (Lactobacillus plantarum).</strong></p><p><em><strong>Keywords – </strong>Prebiotics, Manalagi mango, Lactobacillus plantarum</em></p>

scholarly journals In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some Traditionally Fermented Ethiopian Food Products

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Guesh Mulaw ◽  
Tesfaye Sisay Tessema ◽  
Diriba Muleta ◽  
Anteneh Tesfaye
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Survival Rates ◽  
Lactobacillus Paracasei ◽  
Probiotic Properties ◽  
Sequence Comparisons

Probiotics are live microorganisms which when consumed in large number together with a food promote the health of the consumer. The aim of this study was to evaluate in vitro probiotic properties of lactic acid bacteria (LAB) isolated from traditional Ethiopian fermented Teff injera dough, Ergo, and Kocho products. A total of 90 LAB were isolated, of which 4 (4.44%) isolates showed 45.35–97.11% and 38.40–90.49% survival rates at pH values (2, 2.5, and 3) for 3 and 6 h, in that order. The four acid-tolerant isolates were found tolerant to 0.3% bile salt for 24 h with 91.37 to 97.22% rate of survival. The acid-and-bile salt-tolerant LAB isolates were found inhibiting some food-borne test pathogenic bacteria to varying degrees. All acid-and-bile-tolerant isolates displayed varying sensitivity to different antibiotics. The in vitro adherence to stainless steel plates of the 4 screened probiotic LAB isolates were ranged from 32.75 to 36.30% adhesion rate. The four efficient probiotic LAB isolates that belonged to Lactobacillus species were identified to the strain level using 16S rDNA gene sequence comparisons and, namely, were Lactobacillus plantarum strain CIP 103151, Lactobacillus paracasei subsp. tolerans strain NBRC 15906, Lactobacillus paracasei strain NBRC 15889, and Lactobacillus plantarum strain JCM 1149. The four Lactobacillus strains were found to be potentially useful to produce probiotic products.

scholarly journals In vitro screening of probiotic properties of Lactobacillus plantarum isolated from fermented milk product

2020 ◽  
Vol 4 (4) ◽  
pp. 213-223
Author(s):  
Soumitra Nath ◽  
Jibalok Sikidar ◽  
Monisha Roy ◽  
Bibhas Deb
Keyword(s):  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Raw Milk ◽  
Well Being ◽  
Ecological Niches ◽  
Haemolytic Activity ◽  
Probiotic Properties ◽  
Milk Product ◽  
Antibiotic Susceptibility Test

Abstract Objectives The screening of traditional fermented products is essential for the assessment of safety, security, and further development of functional foods for the well-being of human health. The aim of the present study was to isolate and identify bacteria from fermented raw milk samples that exhibit health benefits upon consumption. Methods In order to confirm the isolates as probiotics, several in vitro assays were conducted to assess the probiotic properties of isolated bacteria. The initial screening includes tolerance to acid, bile, pancreatin, and NaCl. The cell surface properties demonstrate their interaction with mucosal epithelium, which includes hydrophobicity and auto-aggregation assay. Safety assessment was done by performing haemolytic test and antibiotic susceptibility test. The antagonistic activity of probiotic strain was further evaluated against some pathogenic bacteria. Results Lactobacillus plantarum (L. plantarum) isolated from fermented raw milk was preliminarily identified by biochemical tests and further confirmed using 16S rRNA identification. The isolate designated as L. plantarum strain GCC_19M1 demonstrated significant tolerance to low pH, 0.3% bile, 0.5% pancreatin, and 5% NaCl. In the presence of simulated gastric juice (at pH 3), the isolate exhibited a survival rate of 93.48–96.97%. Furthermore, the development of ecological niches in the human gut and their successful accumulation have been revealed by auto-aggregation and hydrophobicity properties. Absence of haemolytic activity ensures the non-virulent nature of the strain. Lactobacillus plantarum strain GCC_19M1 showed susceptibility towards gentamicin, tetracycline, kanamycin, meropenem, and ceftriaxone and exhibited an antagonistic effect on pathogenic bacteria. Conclusion The obtained results conveyed that L. plantarum strain GCC_19M1 has strong probiotic potential, and its presence in the fermented raw milk products may serve as a potent functional probiotic food.

scholarly journals In vitro evaluation of probiotic properties of lactic acid bacteria isolated from some traditionally fermented Ethiopian food products

2019 ◽  
Author(s):  
Guesh Mulaw ◽  
Tesfaye Sisay ◽  
Diriba Muleta ◽  
Anteneh Tesfaye
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Lactobacillus Paracasei ◽  
Steel Plates ◽  
Probiotic Properties ◽  
Sequence Comparisons

AbstractProbiotics are live microorganisms which when consumed in large number together with a food promote the health of the consumer. The aim of this study was to evaluatein vitroprobiotic properties of lactic acid bacteria (LAB) isolated from traditional Ethiopian fermentedTeff injeradough,ErgoandKochoproducts. A total of 90 LAB were isolated, of which 4 (4.44%) isolates showed 45.35-97.11% and 38.40-90.49% survival rate at pH values (2, 2.5 and 3) for 3 and 6 h in that order. The four acid tolerant isolates were found tolerant to 0.3% bile salt for 24 h with 91.37 to 97.22% rate of survival. The acid-and-bile salt tolerant LAB isolates were found inhibiting some foodborne test pathogenic bacteria to varying degrees. All acid-and-bile tolerant isolates displayed varying sensitivity to different antibiotics. Thein vitroadherence to stainless steel plates of the 4 screened probiotic LAB isolates were ranged from 32.75 to 36.30% adhesion rate. The four efficient probiotic LAB isolates that belonged toLactobacillusspecies were identified to strain level using 16S rDNA gene sequence comparisons and namely wereLactobacillus plantarumstrain CIP 103151,Lactobacillus paracaseisubsp. tolerans strain NBRC 15906,Lactobacillus paracaseistrain NBRC 15889 andLactobacillus plantarumstrain JCM 1149. The fourLactobacillusstrains were found to have potentially useful to produce probiotic products.

scholarly journals UJI POTENSI PROBIOTIK Lactobacillus plantarium SECARA IN-VITRO

ALCHEMY ◽  
2013 ◽  
Author(s):  
Anik Maunatin ◽  
Khanifa Khanifa
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bile Salt ◽  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Growth Efficiency ◽  
Salmonella Typhi ◽  
Probiotic Potential ◽  
Number Of Cells

<p>Probiotic is a product containing non-pathogenic microbes live, which was given to animals or humans to fix the rate of growth, efficiency, and increase conversion ration of animal or human health by affecting positively the balance of the gut microbes and microbial pathogen control in the digestive tract. In order to potentially lactic acid bacteria as probiotic candidates must pass the test selection among others such as probiotic test resistance to acid pH, the bile salts, against pathogenic bacteria. This experimental research methods for descriptive use that aim to provide information about the probiotic potential  of Lactobacillus plantarum was isolated from the small intestine Mojosari duck (<em>Anasplathyrinchos</em>) with the tests in vitro i.e. the resistance of <em>Lactobacillus plantarum</em> on the condition of the acidicpH (2, 3, and 4), bile salt concentration of 0.3% (b/v), inhibition of pathogenic bacteria (<em>Escherichia coli, Staphylococcus aureus</em> and <em>Salmonella typhi</em>) The results showed that on pH 2 the number of cells of bacteria that lives was 4.3. 10<sup>7</sup> CFU/ml, pH 3 was  3.8. 10<sup>9</sup> CFU/ml and pH 4 was 2.7. 10<sup>10</sup> CFU/ml, the resistance of <em>Lactobacillus plantarum</em> on bile salt showed the number of cells of bacteria that lives was 1.2. 10<sup>9</sup> CFU/ml.inhibition of the pathogenic bacteria showed that<em>Lactobacillus plantarum</em>could inhibited strong on Escherichia coli and Staphylococcus aureus with diameter of zone was 12.7 mm and 13.3 mm but not<em>Salmonellatyphi</em> with diameter of zone was 9.3 mm.</p>

scholarly journals In Vitro Prebiotic Effects of Malto-Oligosaccharides Containing Water-Soluble Dietary Fiber

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5201
Author(s):  
Eun Yeong Jang ◽  
Ki-Bae Hong ◽  
Yeok Boo Chang ◽  
Jungcheul Shin ◽  
Eun Young Jung ◽  
...  
Keyword(s):  
Short Chain Fatty Acid ◽  
Carbon Sources ◽  
Basal Medium ◽  
In Vitro Digestion ◽  
Fecal Microbiota ◽  
Water Soluble ◽  
Beneficial Bacteria ◽  
Soluble Dietary Fiber ◽  
In Vitro Fermentation

This study measured the proliferative activity of malto-oligosaccharide (MOS) as a prebiotic against Bifidobacteria, resistance to digestion in vitro, and changes during in vitro fermentation by human fecal microorganisms. It consisted of 21.74%, 18.84%, and 11.76% of maltotriose, maltotetraose, and maltopentaose produced by amylase (HATT), respectively. When 1% of MOS was added to a modified PYF medium as the carbon source, proliferation of Bifidobacterium breve was increased significantly. During the in vitro digestion test, MOS was partially degraded by intestinal enzymes. Fermentation characteristics by human fecal microorganisms were evaluated by adding 1% galacto-oligosaccharide (GOS), as well as 1% and 2% MOS as carbon sources to the basal medium, respectively. In comparison with the addition of 1% of MOS and GOS, the total short chain fatty acid (SCFA) content increased over time when 2% of MOS was added. The species diversity and richness of intestinal microbiota increased significantly with 2% MOS compared to those with 1% GOS. In addition, the 2% addition of MOS reduced intestinal pathobiont microorganisms and increased commensal microorganisms including Bifidobacterium genus. Collectively, MOS produced by amylase increased the SCFA production and enhanced the growth of beneficial bacteria during in vitro fermentation by human fecal microbiota.

scholarly journals KAJIAN ISOLAT BAKTERI ASAM LAKTAT DALAM MENURUNKAN KOLESTEROL SECARA IN VITRO DENGAN KEBERADAAN OLIGOSAKARIDA (A Study In Vitro of Lactic Acid Bacteria (LAB) Isolates on Cholesterol Lowering Ability in The Presence of Oligosaccharides)

2016 ◽  
Vol 36 (02) ◽  
pp. 196 ◽  
Author(s):  
Yati Maryati ◽  
Lilis Nuraida ◽  
Ratih Dewanti Hariyadi
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Carbon Source ◽  
Cell Surface ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Acidophilus ◽  
Lactobacillus Rhamnosus ◽  
Cholesterol Reduction ◽  
Cholesterol Binding

This work evaluated the abilities of five isolates of lactic acid bacteria (LAB) from different sources, i.e Lactobacillus fermentum S21209 and Lactobacillus plantarum 1-S27202 from tempe, Lactobacillus rhamnosus R23 and Pediococcus pentosaceus 1-A38 from human breast milk and a commercially available human isolates Lactobacillus acidophilus FNCC0051 in lowering cholesterol by in vitro and metabolizing the prebiotic oligosaccharide compounds. The effects of oligosaccharide compounds on the performance of the LAB isolates in lowering cholesterol were also evaluated. The tests were done in MRS based medium in vitro with or without oligosaccharides i.e. galactooligosacharrides (GOS), fructooligosaccharides (FOS), inulin, hydrolyzed inulin or combination of oligosaccharides as prebiotics. The results revealed that all isolates were able to reduce cholesterol in the medium, and the highest cholesterol reduction was observed for L. acidophilus FNCC0051 and L. rhamnosus R23. There are two different mechanism in the loweringof cholesterol; cholesterol assimilation and cholesterol binding on the cell surface. For the case of P. pentosaceus 1-A38, it involves the assimilation, while the other four isolates may involve cholesterol binding on the cell surface. In addition, the tested LAB’s has different ability to use prebiotics, as shown by the reduction of total sugar in the medium. Oligosaccharides metabolism by L. acidophilus FNCC0051 and L. rhamnosus R23 resulted in several organic acid and SCFA with lactic acid produced as the largest proportion followed by acetic acid. Furthermore, the proportion of propionic and butyric acids were influenced by the type of isolates and carbon source. L. acidophilus FNCC 0051 was able to reduce cholesterol in the MRS based medium with oligosaccharides and their combination as carbon source and cholesterol reducing ability seems to involve both assimilation and cholesterol binding on the cell surface.Keywords: Lactic acid bacteria (LAB), oligosaccharides, synbiotic, cholesterol reduction, prebioticsABSTRAKPenelitian ini mengevaluasi lima isolat bakteri asam laktat (BAL) dari sumber yang berbeda, yaitu Lactobacillus fermentum S21209 dan Lactobacillus plantarum 1-S27202 dari tempe, Lactobacillus rhamnosus R23 dan Pediococcus pentosaceus 1-A38 dari ASI dan isolat komersial Lactobacillus acidophilus FNCC 0051 dari percernaan manusia dalam kemampuannya menurunkan kolesterol secara in vitro dan kemampuannya memetabolisme senyawa oligosakarida prebiotik. Pengaruh senyawa oligosakarida terhadap kemampuan isolat BAL terpilih untuk menurunkan kolesterol juga dievaluasi. Pengujian dilakukan pada media berbasis MRS dengan atau tanpa oligosakarida terdiri dari galaktooligosakarida (GOS), fruktooligosakarida (FOS), inulin, hidrolisat inulin atau kombinasi oligosakarida sebagai prebiotik. Hasil penelitian menunjukkan bahwa semua isolat mampu menurunkan kolesterol, dan penurunan kolesterol tertinggi ditunjukkan oleh isolat L. acidophilus FNCC0051 dan L. rhamnosus R23. Penurunan kolesterol diduga terjadi melalui dua cara yang berbeda. Mekanisme penurunan kolesterol oleh isolat P. pentosaceus 1-A38 melibatkan asimilasi kolesterol, sedangkan pada keempat isolat lainnya kemungkinan melibatkan pengikatan kolesterol pada permukaan sel. Selain itu, isolat BAL juga memiliki kemampuan yang berbeda dalam memanfaatkan oligosakarida prebiotik, terlihat pada penurunan total gula dalam medium. Metabolisme senyawa oligosakarida oleh L. acidophilus FNCC0051 dan L. rhamnosus R23 menghasilkan beberapa asam organik termasuk SCFA dengan proporsi terbesar asam laktat diikuti oleh asam asetat. Selain itu, proporsi asam propionat dan butirat dipengaruhi oleh jenis isolat dan sumber karbon. L. acidophilus FNCC 0051 mampu menurunkan kolesterol dalam media berbasis MRS dengan keberadaan oligosakarida baik tunggal maupun kombinasi sebagai sumber karbon dan melibatkan mekanisme baik asimilasi dan pengikatan kolesterol pada permukaan sel.Kata kunci: Bakteri asam laktat (BAL), oligosakarida, sinbiotik, penurunan kolesterol, prebiotik

scholarly journals Potensi Antagonistik Bakteri Lactobacillus plantarum Terhadap Bakteri Patogen Aeromonas salmonicida Secara In Vitro [The Potential Antagonistic Bacterium Lactobacillus plantarum Against Bacterial Pathogens Aeromonas salmonicida By In Vitro]

2019 ◽  
Vol 5 (2) ◽  
pp. 211
Author(s):  
Yunifar Amad, Rahayu Kusdarwati, Gunanti Mahasri
Keyword(s):  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Bacterial Pathogens ◽  
Bacterial Pathogen ◽  
Aeromonas Salmonicida ◽  
Randomized Design ◽  
Antagonistic Potential ◽  
Completely Randomized Design ◽  
Paper Disc

Abstract Problems were often experienced by fish farmers is bacterial pathogen Aeromonas salmonicida which causes furunculosis disease in fish. The way to control the growth of this pathogenic bacteria was using antagonist bacteria of Lactobacillus plantarum. Growth of L. plantarum may inhibit contamination of pathogenic bacterial because of its ability to produce bacteriocins, produce lactic, moreover that these bacteria can produce hydrogen peroxide which can function as an antibacterial. This research aimed to provide information on the use of bacterial antagonists L. plantarum in inhibiting the growth of pathogenic bacteria A. salmonicida on In Vitro. This research was conducted on October 2012 until January 2013 at dry laboratory in Fisheries and Marine Faculty of Airlangga University Surabaya. This research used Completely Randomized Design (CRD) with five treatments and four replications. Bacteria L. plantarum with 0 concentration for control (A), 106 (B), 107 (C), 108 (D), 109 (E) tested challenge by paper disc method with 106 concentrate of pathogenic bacteria A. salmonicida in vitro. The results showed that L. plantarum with a concentration of 109 CFU/ml (E) was a treatment that produced average of obstacle on the distribution of A. salmonicida amounted to 12,375 mm. Then the treatment with a concentration of 108 CFU/ml (D) with the average of obstacle 8,95 mm. While treatment with a concentration of 107 CFU/ml (C) and 106 CFU/ml (B) was having average of obstacle 6.8 mm and 6 mm was not significantly different from the control (A) which had an average 6 mm obstacle. So it could be concluded that bacteria L. plantarum had antagonistic potential against bacterial pathogens A.salmonicida which indicated by the obstacle produced by L. plantarum on growth of pathogenic bacteria A.salmonicida.

Preliminary in Vitro Investigations on The Inhibitory Activity of The Original Dietary Supplement Oxidal® on Pathogenic Bacterial Strains

2020 ◽  
Vol 11 ◽  
pp. 37-43
Author(s):  
Prof. Teodora P. Popova ◽  
Toshka Petrova ◽  
Ignat Ignatov ◽  
Stoil Karadzhov
Keyword(s):  
Dietary Supplement ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Clinical Effectiveness ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Microbial Strains

The antimicrobial action of the dietary supplement Oxidal® was tested using the classic Bauer and Kirby agar-gel diffusion method. Clinical and reference strains of Staphylococcus aureus and Escherichia coli were used in the studies. The tested dietary supplement showed a well-pronounced inhibitory effect against the microbial strains commensurable with that of the broad-spectrum chemotherapeutic agent Enrofloxacin and showed even higher activity than the broad spectrum antibiotic Thiamphenicol. The proven inhibitory effect of the tested dietary supplement against the examined pathogenic bacteria is in accordance with the established clinical effectiveness standards for antimicrobial agents.

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