URINARY EXCRETION OF TGF-Β1 AND VEGF IN CHILDREN WITH VESICOURETERAL REFLUX

2020 ◽  
Vol 73 (11) ◽  
pp. 2411-2415
Author(s):  
Natalia I. Makieieva ◽  
Oksana O. Morozova ◽  
Kateryna K. Iarova ◽  
Yulianna S. Pryima ◽  
Viktoriia O. Golovachova ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Early Diagnosis ◽  
Roc Analysis ◽  
Renal Scarring ◽  
Control Group ◽  
Urine Excretion ◽  
Statistical Parameters ◽  
Non Invasive ◽  
Urine Levels ◽  
Tgf Β1

The aim of this study was to investigate the relation between urinary TGF-β1, urinary VEGF and renal scarring resulted from VUR. Materials and methods: This study included 141 patients with VUR and 34 healthy sex and age matched children. The statistical analysis consisted of descriptive statistical parameters, KruskalWallis, Mann-Whitney tests and ROC analysis. Results: The urine levels of TGF-β1 and VEGF were significantly increased in children with VUR, compared to the controls. The levels of TGF-β1 urine excretion in children with renal scarring were higher compared children no renal scarring. The indicators of VEGF urine excretion in children with renal scarring compared to indicators in children no renal scarring, were lower, however exceeded the indicators in children of control group. The area under the ROC curve for TGF-β1 was 109.9, for VEGF was 207.6. Conclusions: The study allowed to substantiate and propose non-invasive methods for early diagnosis of renal scarring in children with VUR.

The usefulness of biomarkers in diagnosis of asbestos-induced malignant pleural mesothelioma

2021 ◽  
pp. 096032712110173
Author(s):  
Zübeyde Tanrıverdi ◽  
Fatih Meteroglu ◽  
Hande Yüce ◽  
Abdurrahman Şenyiğit ◽  
Mümtaz Işcan ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Early Diagnosis ◽  
Malignant Pleural Mesothelioma ◽  
Asbestos Exposure ◽  
Diagnostic Value ◽  
Control Group ◽  
Pleural Mesothelioma ◽  
Spearman Correlation ◽  
Non Invasive ◽  
Noninvasive Markers

Introduction: Malignant pleural mesothelioma (MPM) is a malignant tumor that is associated mostly with asbestos exposure. The present study was to evaluates the diagnostic value of neopterin, periostin, YKL-40, Tenascin-C (TNC), and Indolamine 2,3-dioxygenase (IDO) as noninvasive markers of malign pleural mesothelioma. Methods: Included in the study were 30 patients diagnosed with malign pleural mesothelioma, and 25 people as a control group. Biomarker levels were determined using an enzyme immunoassay . A Mann-Whitney U test and Spearman correlation methods were used for the statistical analysis. Results: All evaluated biomarkers were found to be significantly higher in the MPM group than in the control group ( p < 0.05). There was no effect of such variables as gender, age or MPMsubtype on the parameters ( p > 0.05) in the patient group. All biomarkers were positively correlated with each other ( p < 0.001). Conclusions: The current non-invasive biomarkers that can be used in the diagnosis of MPM yielded significant results and can make important contributions to the early diagnosis of MPM.

scholarly journals Screening method for diagnosis of pharyngolaryngeal reflux

2021 ◽  
Vol 23 (3) ◽  
pp. 236-239
Author(s):  
Marina A. Ryabova ◽  
◽  
Natalia A. Shumilova ◽  
Liubov V. Georgieva ◽  
Artem V. Tishkov ◽  
...  
Keyword(s):  
Roc Analysis ◽  
Ph Monitoring ◽  
Screening Method ◽  
Control Group ◽  
Study Group ◽  
Upper Respiratory Tract ◽  
Non Invasive ◽  
Patient Complaints ◽  
Invasive Method ◽  
Upper Respiratory

Pharyngolaryngeal reflux (PLR) can cause the upper respiratory tract pathology. To diagnose PLR based on complaints is rather difficult, due to the absence of typical manifestations of reflux syndrome in some cases of the disease. The use of daily impedance pH monitoring of the esophagus is not always possible due to the invasiveness and unavailability of the method. The aim of the study was to develop an affordable non-invasive method for diagnosing PLR. The study group consisted of 48 patients with PLR proven by the results of impedance pH-monitoring. The control group consisted of 20 healthy patients. The “Pharyngolaryngeal reflux index” questionnaire was developed based on the analysis of patient complaints. The questionnaire was assessed using ROC analysis. Patients who scored 5.5 points according to the results of the questionnaire with a probability of 93.2% have PLR. Thus, a method for the diagnosis of PLR has been developed, including the simultaneous comparison of the laryngoscopic signs of PLR with the highest diagnostic sensitivity (hyperemia, edema, maceration of the interarytenoid mucosa) and “Pharyngolaryngeal reflux index” questionnaire results, which allows diagnosing of PLR.

scholarly journals Can SCUBE1 be used to predict the early diagnosis, lesion volume and prognosis of acute ischemic stroke?

2018 ◽  
Vol 44 (1) ◽  
pp. 16-24
Author(s):  
Asli Bolayir ◽  
Seyda Figul Gokce ◽  
Burhanettin Cigdem ◽  
Hasan Ata Bolayir ◽  
Aydin Gulunay ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Early Diagnosis ◽  
Acute Ischemic Stroke ◽  
Roc Analysis ◽  
High Sensitivity ◽  
Control Group ◽  
Lesion Volume ◽  
Epidermal Growth ◽  
Independent Effects ◽  
Prediction Of Prognosis

Abstract Objective SCUBE1 [signal peptide-CUB (complement C1r/C1 s)-EGF (epidermal growth factor)-like domain-containing protein 1] is a novel biochemical marker. SCUBE1 is thought to play roles both in platelet activation and inflammation, which are important stages for the development of acute ischemic stroke (AIS).The purpose of our study was to determine the diagnostic and prognostic values and temporal change of plasma SCUBE1 levels in AIS patients. Materials and methods Thirty-five patients diagnosed with AIS at the Cumhuriyet University Faculty of Medicine Neurology Department, between June and December 2017, and a control group of 35 healthy volunteers were included. Results Median first day SCUBE1 value in the patient group was 97.51 ng/mL, and the median 7th day SCUBE1 value was 32.72 ng/mL. Median control group SCUBE1 value was 27.51 ng/mL. The first day SCUBE1 levels were significantly higher than the 7th day and the control group SCUBE1 levels (p=0.001, p<0.001, respectively). The ROC analysis showed that SCUBE1 levels above 68.8 ng/mL can be used as an indicator with high sensitivity and specificity for AIS diagnosis. Multivariate analysis revealed that the first day SCUBE1 had significant independent effects on development of AIS. In correlation analysis, plasma SCUBE1 levels showed a significantly positive correlation with lesion volume, NIHSS and MRS values (p<0.01). Conclusion First day plasma SCUBE1 values in AIS patients rised at significant levels compared to the control group. SCUBE1 could use both in the early diagnosis and prediction of prognosis and lesion volume of AIS patients.

scholarly journals The Usefulness of Urinary Periostin, Cytokeratin-18, and Endoglin for Diagnosing Renal Fibrosis in Children with Congenital Obstructive Nephropathy

2021 ◽  
Vol 10 (21) ◽  
pp. 4899
Author(s):  
Agnieszka Turczyn ◽  
Małgorzata Pańczyk-Tomaszewska ◽  
Grażyna Krzemień ◽  
Elżbieta Górska ◽  
Urszula Demkow
Keyword(s):  
Chronic Kidney Disease ◽  
Renal Fibrosis ◽  
Roc Analysis ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Obstructive Nephropathy ◽  
Study Group ◽  
Cytokeratin 18 ◽  
Non Invasive ◽  
Tgf Β1

Congenital obstructive nephropathy (CON) leads to renal fibrosis and chronic kidney disease. The aim of the study was to investigate the predictive value of urinary endoglin, periostin, cytokeratin-18, and transforming growth factor-β1 (TGF-β1) for assessing the severity of renal fibrosis in 81 children with CON and 60 controls. Children were divided into three subgroups: severe, moderate scars, and borderline lesions based on 99mTc-ethylenedicysteine scintigraphy results. Periostin, periostin/Cr, and cytokeratin-18 levels were significantly higher in the study group compared to the controls. Children with severe scars had significantly higher urinary periostin/Cr levels than those with borderline lesions. In multivariate analysis, only periostin and cytokeratin-18 were independently related to the presence of severe and moderate scars, and periostin was independently related to borderline lesions. However, periostin did not differentiate advanced scars from borderline lesions. In ROC analysis, periostin and periostin/Cr demonstrated better diagnostic profiles for detection of advanced scars than TGF-β1 and cytokeratin-18 (AUC 0.849; 0.810 vs. 0.630; 0.611, respectively) and periostin for detecting borderline lesions than endoglin and periostin/Cr (AUC 0.777 vs. 0.661; 0.658, respectively). In conclusion, periostin seems to be a promising, non-invasive marker for assessing renal fibrosis in children with CON. CK-18 and TGF-β1 demonstrated low utility, and endoglin was not useful for diagnosing advanced scars.

PENGARUH PEMBERIAN FRAKSI ETILASETAT KULIT UBI JALAR UNGU TERHADAP KADAR MALONDIALDEHID (MDA) SERUM MENCIT PUTIH JANTAN HIPERGLIKEMIA

2018 ◽  
Vol 8 (2) ◽  
pp. 144
Author(s):  
Ria Afrianti
Keyword(s):  
Blood Glucose ◽  
Statistical Analysis ◽  
Sweet Potato ◽  
Ethyl Acetate Fraction ◽  
Serum Levels ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Purple Sweet Potato

This study aims to determine the effect giving of ethylacetate fraction of leather  purple sweet potato (Ipomoea batatas (L.) Lam, on levels of malondialdehyde (MDA) serum in mice hyperglicemia were induced with streptozocin dose of 50 mg/kgBW. Mice were divided into 5 groups, each group consisting of 3 tails, group I is a negative control, group II is a positive control, group III,IV and V is given ethylacetate fraction a dose of 100 mg/kgBW, 300 mg/kgBW, and 600 mg/kgBW. Ethyl Acetate Fraction leather purple sweet potato given orally for 15 days after the animal is declared hyperglicemia and measurement of blood glucose levels on 5, 10, and 15 day after giving test preparation in animal experiments. On the 16 day throughout the mice were taken serum levels measured malondialdehid. The statistical analysis results showed that giving of ethyl acetate fraction of leather purple sweet potato at a dose of 100 mg/kgBW, 300 mg/kgBW, and 600 mg/kgBW can lower blood glucose levels in mice hyperglycemia significantly (p<0.05). Malondialdehid levels on average in each group is 1.35 nmol/ml, 3.00 nmol/ml, 2.72 nmol/ml, 2.20 nmol/ml and 2.61 nmol/ml, the results of statistical analysis showed a decrease in melondialdehid serum levels were significantly (p<0.05), where a dose of 300 mg/kgBW is an effective dose for lowering blood glucose levels followed by decreased levels of malondialdehid which give effect approaching negative control.

Relationship between Invasive and Non-Invasive Hemodynamic Measures in Experimental Pulmonary Hypertension

2020 ◽  
Vol 16 (1) ◽  
pp. 47-53
Author(s):  
Vicente Benavides-Córdoba ◽  
Mauricio Palacios Gómez
Keyword(s):  
Heart Rate ◽  
Pulmonary Hypertension ◽  
Ejection Fraction ◽  
Right Ventricle ◽  
Correlation Coefficient ◽  
Pearson Correlation ◽  
Systolic Pressure ◽  
Control Group ◽  
Pearson Correlation Coefficient ◽  
Non Invasive

Introduction: Animal models have been used to understand the pathophysiology of pulmonary hypertension, to describe the mechanisms of action and to evaluate promising active ingredients. The monocrotaline-induced pulmonary hypertension model is the most used animal model. In this model, invasive and non-invasive hemodynamic variables that resemble human measurements have been used. Aim: To define if non-invasive variables can predict hemodynamic measures in the monocrotaline-induced pulmonary hypertension model. Materials and Methods: Twenty 6-week old male Wistar rats weighing between 250-300g from the bioterium of the Universidad del Valle (Cali - Colombia) were used in order to establish that the relationships between invasive and non-invasive variables are sustained in different conditions (healthy, hypertrophy and treated). The animals were organized into three groups, a control group who was given 0.9% saline solution subcutaneously (sc), a group with pulmonary hypertension induced with a single subcutaneous dose of Monocrotaline 30 mg/kg, and a group with pulmonary hypertension with 30 mg/kg of monocrotaline treated with Sildenafil. Right ventricle ejection fraction, heart rate, right ventricle systolic pressure and the extent of hypertrophy were measured. The functional relation between any two variables was evaluated by the Pearson correlation coefficient. Results: It was found that all correlations were statistically significant (p <0.01). The strongest correlation was the inverse one between the RVEF and the Fulton index (r = -0.82). The Fulton index also had a strong correlation with the RVSP (r = 0.79). The Pearson correlation coefficient between the RVEF and the RVSP was -0.81, meaning that the higher the systolic pressure in the right ventricle, the lower the ejection fraction value. Heart rate was significantly correlated to the other three variables studied, although with relatively low correlation. Conclusion: The correlations obtained in this study indicate that the parameters evaluated in the research related to experimental pulmonary hypertension correlate adequately and that the measurements that are currently made are adequate and consistent with each other, that is, they have good predictive capacity.

scholarly journals Retinal vascular fractal dimensions and their association with macrovascular cardiac disease.

2021 ◽  
Author(s):  
Sebastian Dinesen ◽  
Pia Søndergaard Jensen ◽  
Maria Bloksgaard ◽  
Søren Leer Blindbæk ◽  
Jo G.R. De Mey ◽  
...  
Keyword(s):  
Confidence Interval ◽  
Cardiac Disease ◽  
Artery Bypass ◽  
Fractal Dimensions ◽  
Macrovascular Disease ◽  
Control Group ◽  
Vascular Structure ◽  
Non Invasive ◽  
Automatic Software ◽  
Multivariable Regression

Introduction As the only part of the human vasculature, retina is available for direct, non-invasive inspection. Retinal vascular fractal dimension (DF) is a method to measure the structure of the retinal vascular tree, with higher non-integer values between 1 and 2 representing a more complex and dense retinal vasculature. Retinal vascular structure has been associated with a variety of systemic diseases and this study examined the association of DF and macrovascular cardiac disease in a case-control design. Methods Retinal fundus photos were captured with Topcon TRC-50X in 38 persons that had coronary artery bypass grafting (CABG, cases) and 37 cardiovascular healthy controls. The semi-automatic software VAMPIRE was used to measure retinal DF. Results Patients with CABG had lower DF of the retinal main venular vessels compared to the control group (1.15 vs. 1.18, p=0.01). In a multivariable regression model adjusted for gender and age, eyes in the fourth quartile with higher DF were less likely to have CABG compared to patients in the first (OR, 7.20; 95% confidence interval, 1.63 to 31.86; p=0.009) and second quartile (OR, 8.25; 95% confidence interval, 1.70 to 40.01; p=0.009). Conclusions This study demonstrates that lower complexity of main venular vessels associates with higher risk of having CABG. The research supports the hypothesis that the retinal vascular structure can be used to assess non-ocular macrovascular disease.

scholarly journals Variability of serum IgG sialylation and galactosylation degree in women with advanced endometriosis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Katarzyna Sołkiewicz ◽  
Hubert Krotkiewski ◽  
Marcin Jędryka ◽  
Ewa M. Kratz
Keyword(s):  
Cluster Analysis ◽  
Sialic Acid ◽  
Control Group ◽  
Clinical Tool ◽  
Non Invasive ◽  
Healthy Women ◽  
Serum Igg ◽  
And Cluster Analysis ◽  
Invasive Diagnostics ◽  
And Control

AbstractEndometriosis is an inflammatory disease which diagnostics is difficult and often invasive, therefore non-invasive diagnostics methods and parameters are needed for endometriosis detection. The aim of our study was to analyse the glycosylation of native serum IgG and IgG isolated from sera of women classified as: with endometriosis, without endometriosis but with some benign ginecological disease, and control group of healthy women, in context of its utility for differentiation of advanced endometriosis from the group of healthy women. IgG sialylation and galactosylation/agalactosylation degree was determined using specific lectins: MAA and SNA detecting sialic acid α2,3- and α2,6-linked, respectively, RCA-I and GSL-II specific to terminal Gal and terminal GlcNAc, respectively. The results of ROC and cluster analysis showed that the serum IgG MAA-reactivity, sialylation and agalactosylation factor may be used as supplementary parameters for endometriosis diagnostics and could be taken into account as a useful clinical tool to elucidate women with high risk of endometriosis development. Additionally, we have shown that the analysis of native serum IgG glycosylation, without the prior time-consuming and expensive isolation of the protein, is sufficient to differentiation endometriosis from a group of healthy women.

scholarly journals The Effect of a Non-Invasive Positive Pressure Ventilation Simulation Program on General Ward Nurses’ Knowledge and Self-Efficacy

2021 ◽  
Vol 18 (6) ◽  
pp. 2877
Author(s):  
Moon-Sook Kim ◽  
Mi-Hee Seo ◽  
Jin-Young Jung ◽  
Jinhyun Kim
Keyword(s):  
Self Efficacy ◽  
Qualitative Data ◽  
Positive Pressure Ventilation ◽  
Intervention Group ◽  
General Ward ◽  
Simulation Program ◽  
Positive Pressure ◽  
Control Group ◽  
Training Strategy ◽  
Non Invasive

The purpose of this study is to develop a simulation-based ventilator training program for general ward nurses and identify its effects. Quantitative data were collected from 29 nurses (intervention group: 15, control group: 14), of which seven were interviewed with focus groups to collect qualitative data. The quantitative results revealed significant differences in ventilator-related knowledge (p = 0.029) and self-efficacy (p = 0.026) between the intervention and control groups. Moreover, three themes were derived from meaningful statements in the qualitative data: understanding psychophysical discomfort of the patient while applying the ventilator; helping in ventilator care; and establishing a future ventilator training strategy. The findings confirmed that the non-invasive positive pressure ventilation (NPPV) simulation program is an effective method for improving the knowledge of ventilator nursing and self-efficacy and will be helpful in developing educational methods and strategies related to ventilator nursing for general ward nurses.

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