scholarly journals Bio-degradation of 40 micron plastic bags by Aspergillus niger and optimization of pre-treatment methods

2013 ◽  
Vol 14 (3) ◽  
pp. 61-68 ◽  
Author(s):  
Sagar Kittur ◽  
Namrata Patil ◽  
Shruti Kammar ◽  
V. G. Shanmuga Priya ◽  
D. N. Sastry

Lack of degradability and the closing of landfill sites as well as growing water and land pollution problems have led to concern about plastics. Among the various types of plastics, the most extensively used type is polyethylene bags. A survey on 40 micron polyethylene bags was done by obtaining the information from the City corporation office, Belgaum, Karnataka, India on the amount of plastic waste generated in the city. Polyethylene bags having a thickness less than 40 micron are not recycled, thus have to be degraded. In this work, Aspergillus niger is been employed to degrade the 40 micron plastic. Various pre-treatment methods are used which include UV, nitric acid, thermal and UV + nitric acid treatments to effectively degrade the plastic. Different media having pre treated plastic as the carbon source are tested for the degradation of plastic along with the growth of the fungi. Incubation period was taken as 15 and 30 days. Optimization of pre-treatment methods was done to improve the degradation efficiency. Various analysis methods such as FTIR spectroscopy and dry-weight analysis were done to confirm the degradation of plastic.

Metals ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 187 ◽  
Author(s):  
Jil Schosseler ◽  
Anna Trentmann ◽  
Bernd Friedrich ◽  
Klaus Hahn ◽  
Hermann Wotruba

This paper focuses on the recycling of silver from spent oxygen-depolarized cathodes through an innovative combination of pre-treatment methods and leaching. A silver- and polytetrafluorethylene (PTFE)-rich fraction was produced by cryogenic milling, screening, and magnetic separation. In order to understand the kinetic leaching mechanism, the silver-rich fraction was leached by different concentrations of nitric acid and hydrogen peroxide. Results showed that nickel influences the silver leaching. This leads to complex reaction systems, which cannot be described by the Arrhenius law.


2020 ◽  
Author(s):  
CRK Glasson ◽  
Ian Sims ◽  
Susan Carnachan ◽  
R de Nys ◽  
M Magnusson

© 2017 Elsevier B.V. We evaluated eight biorefinery processes targeting the extraction of ulvan from Ulva ohnoi. Using a factorial design the effect of three sequential treatments (aqueous extraction of salt; ethanol extraction of pigments; and Na2C2O4 or HCl (0.05 M) extraction of ulvan) were evaluated based on the yield (% dry weight of biomass) and quality (uronic acid, sulfate, protein and ash content, constituent sugar and molecular weight analysis) of ulvan extracted. The aqueous extraction of salt followed by HCl extraction of ulvan gave higher yields (8.2 ± 1.1% w/w) and purity of ulvan than equivalent Na2C2O4 extracts (4.0 ± 1.0% w/w). The total sugar content of HCl extracts (624–670 μg/mg) was higher than Na2C2O4 extracts (365–426 μg/mg) as determined by constituent sugar with ulvan specific monosaccharides contributing 94.7–96.2% and 70.1–84.0%, respectively. Ulvan extracted from U. ohnoi was 53.1 mol% rhamnose, 27.8 mol% glucuronic acid, 10.1 mol% iduronic acid, and 5.3 mol% xylose with molecular weights ranging from 10.5–312 kDa depending on the biorefinery process employed. Therefore, the extraction of high quality ulvan from U. ohnoi is facilitated by an aqueous pre-treatment and subsequent HCl-extraction of ulvan as part of a cascading biorefinery model delivering salt, ulvan, and a protein enriched residual biomass.


2020 ◽  
Author(s):  
CRK Glasson ◽  
Ian Sims ◽  
Susan Carnachan ◽  
R de Nys ◽  
M Magnusson

© 2017 Elsevier B.V. We evaluated eight biorefinery processes targeting the extraction of ulvan from Ulva ohnoi. Using a factorial design the effect of three sequential treatments (aqueous extraction of salt; ethanol extraction of pigments; and Na2C2O4 or HCl (0.05 M) extraction of ulvan) were evaluated based on the yield (% dry weight of biomass) and quality (uronic acid, sulfate, protein and ash content, constituent sugar and molecular weight analysis) of ulvan extracted. The aqueous extraction of salt followed by HCl extraction of ulvan gave higher yields (8.2 ± 1.1% w/w) and purity of ulvan than equivalent Na2C2O4 extracts (4.0 ± 1.0% w/w). The total sugar content of HCl extracts (624–670 μg/mg) was higher than Na2C2O4 extracts (365–426 μg/mg) as determined by constituent sugar with ulvan specific monosaccharides contributing 94.7–96.2% and 70.1–84.0%, respectively. Ulvan extracted from U. ohnoi was 53.1 mol% rhamnose, 27.8 mol% glucuronic acid, 10.1 mol% iduronic acid, and 5.3 mol% xylose with molecular weights ranging from 10.5–312 kDa depending on the biorefinery process employed. Therefore, the extraction of high quality ulvan from U. ohnoi is facilitated by an aqueous pre-treatment and subsequent HCl-extraction of ulvan as part of a cascading biorefinery model delivering salt, ulvan, and a protein enriched residual biomass.


2002 ◽  
Vol 16 (2) ◽  
pp. 113-120 ◽  
Author(s):  
S.K. Yadav ◽  
S. Sehgal

Spinach ( Spinacia oleracia) and amaranth ( Amaranthus tricolor) leaves were stored in polyethylene bags and without packing for 24 and 48 hours in a refrigerator at 5°C and 30°C in polyethylene bags. The fresh leaves were also dried (oven and sun), blanched (5, 10 and 15 min) and cooked in an open pan and a pressure cooker. The processed leaves were analysed for total iron, its availability and antinutrient content. The iron content of these leaves varied from 26.54 to 34.14 mg/l00g, dry weight and its HCl-extractability and in vitro availability were 62.11–67.18% and 3.03–3.97% of total respectively. Drying and storage had no significant effect on total iron content, Hel-extractability and availability ( in vitro), while blanching and cooking resulted in significant improvement of iron availability, and a significant reduction in oxalic acid content, while only blanching significantly reduced phytic acid and polyphenol contents. Thus cooking and blanching are good ways to improve HCl-extractability and in vitro availability of iron.


1996 ◽  
Vol 42 (6) ◽  
pp. 533-538 ◽  
Author(s):  
A. R. Podile ◽  
A. P. Prakash

A biocontrol rhizobacterial strain of Bacillus subtilis AF 1 grown for 6 h was coinoculated with Aspergillus niger at different time intervals and microscopic observations revealed adherence of bacterial cells to the fungal mycelium. Bacterial cells multiplied in situ and colonized the mycelial surface. Growth of AF 1 resulted in damage to the cell wall, followed by lysis. AF 1 inoculation into media containing A. niger at 0, 6, and 12 h suppressed >90% fungal growth, while in 18- and 24-h cultures fungal growth inhibition was 70 and 56%, respectively, in terms of dry weight. In dual culture the fungal growth was not accompanied by formation of spores. The mycelial preparation of A. niger as principal carbon source supported the growth of B. subtilis, as much as chitin. Extracellular protein precipitate from B. subtilis culture filtrate had a significant growth-retarding effect on A. niger. Groundnut seeds bacterized with B. subtilis showed a reduced incidence of crown rot in A. niger infested soil, suggesting a possible role of B. subtilis in biological control of A. niger.Key words: mycolytic bacteria, Bacillus subtilis, Aspergillus niger, biological control.


1984 ◽  
Vol 35 (2) ◽  
pp. 115 ◽  
Author(s):  
DJ Mares

Germinability in harvest-mature wheat grain showed a marked dependence on temperature. The optimum temperature for the complete germination of all grains ranged from 20�C for the non-dormant variety, Timgalen, to 10�C for the strongly dormant red wheat RL 4137, whereas the optimum in terms of the shortest lag period ranged from 25� to 15�C for the same varieties. Germinability gradually increased during post-harvest storage and, for after-ripened grain, the optimum temperature for both complete germination and shortest lag period were greater than 30�C. Germinability could also be increased by pre-treating imbibing grains at temperatures of 5�, 10� or in some cases 15�C. This treatment was only effective for grain at moisture contents >25% (dry weight) and the effect was not reversed by redesiccation. The pre-treatment temperature required for maximum germinability decreased with increasing levels of grain dormancy. Complete removal of dormancy required a pre-treatment period of c. 48 h; however, lesser periods gave the shortest lag period in the case of the dormant varieties. The implications of these results for the utilization of dormancy in the development of preharvest sprouting damage tolerant varieties and their subsequent use in practice are discussed.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Leilah Krounbi ◽  
Akio Enders ◽  
John Gaunt ◽  
Margaret Ball ◽  
Johannes Lehmann

AbstractThe conversion of dairy waste with high moisture contents to dry fertilizers may reduce environmental degradation while lowering crop production costs. We converted the solid portion of screw-pressed dairy manure into a sorbent for volatile ammonia (NH3) in the liquid fraction using pyrolysis and pre-treatment with carbon dioxide (CO2). The extractable N in manure biochar exposed to NH3 following CO2 pre-treatment reached 3.36 g N kg−1, 1260-fold greater extractable N than in untreated manure biochar. Ammonia exposure was 142-times more effective in increasing extractable N than immersing manure biochar in the liquid fraction containing dissolved ammonium. Radish and tomato grown in horticultural media with manure biochar treated with CO2 + NH3 promoted up to 35% greater plant growth (dry weight) and 36–83% greater N uptake compared to manure biochar alone. Uptake of N was similar between plants grown with wood biochar exposed to CO2 + NH3, compared to N-equivalent treatments. The available N in dairy waste in New York (NY) state, if pyrolyzed and treated with NH3 + CO2, is equivalent to 11,732–42,232 Mg N year−1, valued at 6–21.5 million USD year−1. Separated dairy manure treated with CO2 + NH3 can offset 23–82% of N fertilizer needs of NY State, while stabilizing both the solid and liquid fraction of manure for reduced environmental pollution.


2000 ◽  
Vol 42 (9) ◽  
pp. 235-241 ◽  
Author(s):  
M. Barjenbruch ◽  
H. Hoffmann ◽  
O. Kopplow ◽  
J. Tränckner

Several reasons can lead to the emergence of foam in digesting tanks, for instance overloading or the impact of hydrophobic substances. Furthermore, the foaming is in regular periods going together with the emergence of filamentous microorganisms. Up to now, several strategies to avoid foaming have been tested out (for instance reduction of the sludge load in the activated sludge stage, lowering of the sludge level in the digestion tank, dosage of anti foaming agents), but these have been done relatively unsystematically and with more or less success. For our contribution, laboratory-scale digestion tests were run to analyse mechanical and thermal pre-treatment methods for the destruction of the surplus sludge. Whereas the disintegration by a high pressure homogeniser did only achieve a low reduction of the foam phase, the thermal pre-treatment at 121°C made for an effective subduing of the foam emergence. Both methods allowed for a cutting up of the filaments, but only the heating up effected the reduction of the hydrophobic substances; thus, the foaming is possibly caused by them.


2017 ◽  
pp. 371-419 ◽  
Author(s):  
Mohd Azmuddin Abdullah ◽  
Muhammad Shahid Nazir ◽  
Huma Ajab ◽  
Safoura Daneshfozoun ◽  
Sakinatu Almustapha

Sign in / Sign up

Export Citation Format

Share Document