Study on Antenna Configuration of Virtual Aperture and Its Associated Imaging Performance

2011 ◽  
Vol 33 (10) ◽  
pp. 2458-2463 ◽  
Author(s):  
Tian Jin ◽  
Jun Lou ◽  
Qian Song ◽  
Zhi-min Zhou
Author(s):  
C J R Sheppard

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.


Author(s):  
R.P. Ferrier ◽  
S. McVitie

Type II magnetic contrast was first observed by Philibert and Tixier and relies on the change in the effective backscattering coefficient due to interaction of the scattered electrons within the specimen and the local magnetic induction (for a review see Tsuno). Depending on the tilt of the specimen and the position of the backscattered electron detector(s), contrast due to the presence of either or both domains and domain walls can be obtained; in the case of the latter, the standard geometry is for the specimen to be normal to the incident beam and the detectors are positioned above it and close to the optic axis. This is the geometry adopted in our studies, which used a JEOL 2000FX with a special split objective lens polepiece; this permitted the specimen to be in magnetic field-free space, the separate lens gaps above and below allowing good probe forming capabilities combined with excellent Lorentz imaging performance. A schematic diagram is shown in Fig. 1.


Author(s):  
H. Rose

The imaging performance of the light optical lens systems has reached such a degree of perfection that nowadays numerical apertures of about 1 can be utilized. Compared to this state of development the objective lenses of electron microscopes are rather poor allowing at most usable apertures somewhat smaller than 10-2 . This severe shortcoming is due to the unavoidable axial chromatic and spherical aberration of rotationally symmetric electron lenses employed so far in all electron microscopes.The resolution of such electron microscopes can only be improved by increasing the accelerating voltage which shortens the electron wave length. Unfortunately, this procedure is rather ineffective because the achievable gain in resolution is only proportional to λ1/4 for a fixed magnetic field strength determined by the magnetic saturation of the pole pieces. Moreover, increasing the acceleration voltage results in deleterious knock-on processes and in extreme difficulties to stabilize the high voltage. Last not least the cost increase exponentially with voltage.


PIERS Online ◽  
2005 ◽  
Vol 1 (5) ◽  
pp. 543-546
Author(s):  
Ho-Jin Lee ◽  
Hyuk Park ◽  
Sung-Hyun Kim ◽  
Yong-Hoon Kim

Sensors ◽  
2021 ◽  
Vol 21 (11) ◽  
pp. 3652
Author(s):  
Cory Juntunen ◽  
Isabel M. Woller ◽  
Yongjin Sung

Hyperspectral three-dimensional (3D) imaging can provide both 3D structural and functional information of a specimen. The imaging throughput is typically very low due to the requirement of scanning mechanisms for different depths and wavelengths. Here we demonstrate hyperspectral 3D imaging using Snapshot projection optical tomography (SPOT) and Fourier-transform spectroscopy (FTS). SPOT allows us to instantaneously acquire the projection images corresponding to different viewing angles, while FTS allows us to perform hyperspectral imaging at high spectral resolution. Using fluorescent beads and sunflower pollens, we demonstrate the imaging performance of the developed system.


Tomography ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 39-54
Author(s):  
Veerle Kersemans ◽  
Stuart Gilchrist ◽  
Philip Danny Allen ◽  
Sheena Wallington ◽  
Paul Kinchesh ◽  
...  

Standardisation of animal handling procedures for a wide range of preclinical imaging scanners will improve imaging performance and reproducibility of scientific data. Whilst there has been significant effort in defining how well scanners should operate and how in vivo experimentation should be practised, there is little detail on how to achieve optimal scanner performance with best practices in animal welfare. Here, we describe a system-agnostic, adaptable and extensible animal support cradle system for cardio-respiratory-synchronised, and other, multi-modal imaging of small animals. The animal support cradle can be adapted on a per application basis and features integrated tubing for anaesthetic and tracer delivery, an electrically driven rectal temperature maintenance system and respiratory and cardiac monitoring. Through a combination of careful material and device selection, we have described an approach that allows animals to be transferred whilst under general anaesthesia between any of the tomographic scanners we currently or have previously operated. The set-up is minimally invasive, cheap and easy to implement and for multi-modal, multi-vendor imaging of small animals.


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