scholarly journals CHARACTERIZATION OF DART SEQUENCES REFLECTING GENOMICREGIONS INVOLVED IN ALUMINUM TOLERANCE IN TRITICALE (X TRITICOSECALE WITTMACK)

2020 ◽  
pp. 39-48
Author(s):  
Agnieszka Niedziela ◽  
Piotr Bednarek
Keyword(s):  
Aluminum Toxicity ◽  
Aluminum Tolerance ◽  
Acid Soils ◽  
Limiting Factor ◽  
Disease Response ◽  
Dna Repair Mechanisms ◽  
Al Stress ◽  
Blast Database ◽  
Repair Mechanisms ◽  
Triticosecale Wittmack

Aluminum toxicity is the major growth-limiting factor for crop cultivation on acid soils. Tolerance mecha-nisms for Al stress in triticale have not been systematically investigated so far. It is presumed, that in the case of this species they may be a function of the interaction between wheat and rye genes. In this study the se-quences of forty-six Diversity Arrays Technology markers associated with aluminum tolerance in triticale and under selection pressure were blasted against BLAST database for the identification of possible functions of the respective genome regions in Al-stress response. The analysis has showed sequences similarity to the domains involved in signaling, disease response and DNA repair mechanisms.

scholarly journals AtHB7/12 Regulate Root Growth in Response to Aluminum Stress

2020 ◽  
Vol 21 (11) ◽  
pp. 4080
Author(s):  
Yang Liu ◽  
Jiameng Xu ◽  
Siyi Guo ◽  
Xianzheng Yuan ◽  
Shan Zhao ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transcription Factors ◽  
Root Growth ◽  
Crop Production ◽  
Expression Patterns ◽  
Acid Soils ◽  
Limiting Factor ◽  
Aluminum Stress ◽  
Al Stress

Aluminum (Al) stress is a major limiting factor for plant growth and crop production in acid soils. At present, only a few transcription factors involved in the regulation of Al resistance have been characterized. Here, we used reversed genetic approach through phenotype analysis of overexpressors and mutants to demonstrate that AtHB7 and AtHB12, two HD-Zip I transcription factors, participate in Al resistance. In response to Al stress, AtHB7 and AtHB12 displayed different dynamic expression patterns. Although both AtHB7 and AtHB12 positively regulate root growth in the absence of Al stress, our results showed that AtHB7 antagonizes with AtHB12 to control root growth in response to Al stress. The athb7/12 double mutant displayed a wild-type phenotype under Al stress. Consistently, our physiological analysis showed that AtHB7 and AtHB12 oppositely regulate the capacity of cell wall to bind Al. Yeast two hybrid assays showed that AtHB7 and AtHB12 could form homo-dimers and hetero-dimers in vitro, suggesting the interaction between AtHB7 and AtHB12 in the regulation of root growth. The conclusion was that AtHB7 and AtHB12 oppositely regulate Al resistance by affecting Al accumulation in root cell wall.

The zinc finger transcription factor ATF1 regulates aluminum tolerance in barley

2020 ◽  
Vol 71 (20) ◽  
pp. 6512-6523
Author(s):  
Liyuan Wu ◽  
Yiyi Guo ◽  
Shengguan Cai ◽  
Liuhui Kuang ◽  
Qiufang Shen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcriptional Activation ◽  
Crop Production ◽  
Molecular Mechanisms ◽  
Aluminum Tolerance ◽  
Acid Soils ◽  
Al Tolerance ◽  
Al Stress ◽  
Transcription Factor 1 ◽  
Differently Expressed Genes

Abstract Aluminum (Al) toxicity is a major abiotic stress that restricts crop production in acid soils. Plants have evolved internal and external mechanisms of tolerance, and among them it is well known that AtSTOP1 and OsART1 are key transcription factors involved in tolerance through regulation of multiple downstream genes. Here, we identified the closest homolog of these two proteins in barley, namely HvATF1, Al-tolerance Transcription Factor 1, and determined its potential function in Al stress. HvATF1 is expressed in the nucleus, and functions in transcriptional activation. The transcription of HvATF1 was found to be constitutive in different tissues, and was little affected by Al stress. Knockdown of HvATF1 by RNAi resulted in increased Al sensitivity. Transcriptomics analysis identified 64 differently expressed genes in the RNAi lines compared to the wild-type, and these were considered as candidate downstream genes regulated by HvATF1. This study provides insights into the different molecular mechanisms of Al tolerance in barley and other plants.

scholarly journals Evaluation of Aluminum Tolerance and Nutrient Uptake of 50 Centipedegrass Accessions and Cultivars

HortScience ◽  
2009 ◽  
Vol 44 (3) ◽  
pp. 857-861 ◽  
Author(s):  
Jun Yan ◽  
Jingbo Chen ◽  
Tingting Zhang ◽  
Jianxiu Liu ◽  
Haibo Liu
Keyword(s):  
Nutrient Uptake ◽  
Aluminum Tolerance ◽  
Acid Soils ◽  
Culture Method ◽  
Solution Culture ◽  
Root Weight ◽  
Preliminary Evaluation ◽  
Al Stress ◽  
Resistant Group ◽  
Sensitive Group

Centipedegrass [Eremochloa ophiuroides (Munro) Hack] is a native grass of China, and information on soil adaptation ranges, including acid soils, among centipedegrass cultivars is limited. Therefore, objectives of this study were 1) to conduct a preliminary evaluation of relative aluminum tolerance of 48 centipedegrass accessions plus a cultivar, TifBlair, and a common centipedegrass under aluminum (Al) stress (0 and 1500 μM Al) by using a solution culture method; and 2) to determine Al effects on nutrient uptake between resistant-group and sensitive-group accessions among the 50 accessions and cultivars. Differences were found among accessions and cultivars, and the CV of relative root weight, relative shoot weight, and relative total weight were 39.9%, 32.9%, and 33.6%, respectively. After growing 28 days in an acid subsoil, the resistant-group accessions showed much better growth than the sensitive-group accessions. The Al concentrations in roots and shoots of the two groups of accessions were increased under Al treatment, but most absorbed Al remained in roots with greater Al absorption among the sensitive group compared with the resistant group. The concentrations of phosphorus (P), magnesium (Mg), calcium (Ca), and potassium (K) in the two groups were reduced under Al stress with reductions of 59.3%, 54.8%, 47.9%, and 41.3% in shoots and reductions of 8.70%, 52.5%, 43.2%, and 34.4% in roots, respectively. Under Al stress, differences in P, Mg, and Ca concentrations were found between the two groups; however, differences were not found for K. The resistant-group accessions maintained higher concentrations of Mg and Ca than the sensitive group.

scholarly journals Enhanced Aluminum Tolerance in Sugarcane: Evaluation of SbMATE Overexpression and Genome-wide Identification of ALMTs in Saccharum Spp.

2020 ◽  
Author(s):  
Ana Paula Ribeiro ◽  
Felipe Vinecky ◽  
KAROLINE ESTEFANI DUARTE ◽  
Thaís Ribeiro Santiago ◽  
Rafael Augusto das Chagas Noqueli Casari ◽  
...  
Keyword(s):  
Sorghum Bicolor ◽  
Aluminum Toxicity ◽  
Root Exudation ◽  
Aluminum Tolerance ◽  
Root Apex ◽  
Limiting Factor ◽  
Potential Candidate ◽  
Al Tolerance ◽  
Tropical Regions ◽  
Genome Wide

Abstract BackgroundA major limiting factor for plant growth is the aluminum (Al) toxicity in acidic soils, especially in tropical regions. The exclusion of Al from the root apex through root exudation of organic acids such as malate and citrate are both the most ubiquitous tolerance mechanisms in the plant kingdom. Two families of anion channels that confer Al tolerance are well described in the literature, ALMT and MATE family. ResultsIn this study, sugarcane plants constitutively overexpressing the Sorghum bicolor MATE gene (SbMATE) showed improved tolerance to Al when compared to non-transgenic (NT) plants, characterized by sustained root growth and exclusion of aluminum from the root apex based on the result obtained with hematoxylin staining. In addition, genome-wide analysis of the recently released sugarcane genome identified 11 ALMT genes and molecular studies showed potential new targets for aluminum tolerance. ConclusionsOur results indicate that the transgenic plants overexpressing the Sorghum bicolor MATE has an improved tolerance to Al. The expression profile of ALMT genes revels potential candidate genes to be used has an alternative for agricultural expansion in Brazil and other areas with aluminum toxicity in poor and acid soils.

scholarly journals Prospecting sugarcane genes involved in aluminum tolerance

2001 ◽  
Vol 24 (1-4) ◽  
pp. 221-230 ◽  
Author(s):  
Rodrigo D. Drummond ◽  
Claudia T. Guimarães ◽  
Juliana Felix ◽  
Fernando E. Ninamango-Cárdenas ◽  
Newton P. Carneiro ◽  
...  
Keyword(s):  
Land Surface ◽  
Expressed Sequence Tag ◽  
Aluminum Toxicity ◽  
Aluminum Tolerance ◽  
Substantial Reduction ◽  
Acid Soils ◽  
Data Bank ◽  
Limiting Factors ◽  
Aluminum Stress ◽  
Major Factors

Aluminum is one of the major factors that affect plant development in acid soils, causing a substantial reduction in yield in many crops. In South America, about 66% of the land surface is made up of acid soils where high aluminum saturation is one of the main limiting factors for agriculture. The biochemical and molecular basis of aluminum tolerance in plants is far from being completely understood despite a growing number of studies, and in the specific case of sugarcane there are virtually no reports on the effects of gene regulation on aluminum stress. The objective of the work presented in this paper was to prospect the sugarcane expressed sequence tag (SUCEST) data bank for sugarcane genes related to several biochemical pathways known to be involved in the responses to aluminum toxicity in other plant species and yeast. Sugarcane genes similar to most of these genes were found, including those coding for enzymes that alleviate oxidative stress or combat infection by pathogens and those which code for proteins responsible for the release of organic acids and signal transducers. The role of these genes in aluminum tolerance mechanisms is reviewed. Due to the high level of genomic conservation in related grasses such as maize, barley, sorghum and sugarcane, these genes may be valuable tools which will help us to better understand and to manipulate aluminum tolerance in these species.

scholarly journals Rapid screening for aluminum tolerance in maize (Zea mays L.)

2000 ◽  
Vol 23 (4) ◽  
pp. 847-850 ◽  
Author(s):  
Carlos Daniel Giaveno ◽  
José B. Miranda Filho
Keyword(s):  
Aluminum Toxicity ◽  
Aluminum Tolerance ◽  
Acid Soils ◽  
Maize Yield ◽  
Rapid Screening ◽  
Intense Staining ◽  
Tropical Regions ◽  
Tolerant Plants ◽  
Hematoxylin Staining ◽  
Two Populations

A significant decrease in maize grain yield due to aluminum toxicity is considered to be one of the most important agricultural problems for tropical regions. Genetic improvement is a useful approach to increase maize yield in acid soils, but this requires a rapid and reliable method to discriminate between genotypes. In our work we investigated the feasibility of using hematoxylin staining (HS) to detect Al-tolerant plants at the seedling stage. The original population along with two populations obtained after one cycle of divergent selection were evaluated by net root growth (NRG) and HS after 7 days in nutrient solution. Results showed a negative correlation between NRG and HS in all populations, in which sensitive plants, characterized by low NRG, exhibited more intense staining than tolerant plants. These results indicate that HS is a useful procedure for selecting Al-tolerant maize seedlings.

scholarly journals New wheat genotypes tolerant to aluminum toxicity obtained by mutation induction

2001 ◽  
Vol 36 (1) ◽  
pp. 61-70 ◽  
Author(s):  
Augusto Tulmann Neto ◽  
Marcelo Correa Alves ◽  
Carlos Eduardo de Oliveira Camargo ◽  
Jairo Lopes de Castro ◽  
Wilson Penteado Ferreira Filho
Keyword(s):  
Grain Yield ◽  
Gamma Ray ◽  
Aluminum Toxicity ◽  
Acid Soils ◽  
Field Trials ◽  
Soil Conditions ◽  
Mutation Induction ◽  
Limiting Factor ◽  
Agronomic Characteristics ◽  
Mutant Lines

Seed from the sensitive wheat (Triticum aestivumL.) cultivar Anahuac was treated to gamma-ray irradiation and eleven Al3+ tolerant mutants selected. The objective was to compare these mutants to the original Anahuac and to the tolerant wheat cultivars IAC-24 and IAC-60 from 1994 to 1996 in acid (Capão Bonito) and limed (Monte Alegre do Sul) soil field trials, in the State of São Paulo, Brazil. Grain yield and agronomic characteristics were analyzed. All the mutant lines yielded higher than the sensitive Anahuac cultivar in the acid soils of Capão Bonito. Under limed soil conditions, 10 mutants had a similar yield to the original sensitive cultivar and one a lower yield. The majority of the mutants were similar in yield to the tolerant cultivars IAC-24 and IAC-60 under both conditions. Some of the mutants showed altered agronomic characteristics, but these alterations did not generally influence the grain yield. The results indicated that tolerant lines with good characteristics may be obtained from a susceptible cultivar by mutation induction, thus allowing cropping under conditions where Al3 + is a limiting factor.

scholarly journals Comparative Analyses Reveal Peroxidases Play Important Roles in Soybean Tolerance to Aluminum Toxicity

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 670
Author(s):  
Juge Liu ◽  
Xiangting Wang ◽  
Ning Wang ◽  
Yang Li ◽  
Ting Jin ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Aluminum Toxicity ◽  
Acid Soils ◽  
Al Toxicity ◽  
Al Tolerance ◽  
Al Stress ◽  
Important Barrier ◽  
Glycine Max L ◽  
Go Terms ◽  
H2o2 Pretreatment

Aluminum (Al) toxicity is an important barrier to soybean (Glycine max (L.) Merr.) production in acid soils. However, little is known about the genes underlying Al tolerance in soybean. We aim to find the key candidate genes and investigate their roles in soybean tolerance to Al toxicity in this study. Comparative transcriptome analyses of the Al-tolerant (KF) and Al-sensitive (GF) soybean varieties under control and Al stress at 6, 12, and 24 h were investigated. A total of 1411 genes showed specific up-regulation in KF or more up-regulation in KF than in GF by Al stress, which were significantly enriched in the GO terms of peroxidase (POD) activity, transporter activity (including the known Al tolerance-related ABC transporter, ALMT, and MATE), and four families of transcription factors (AP2, C3H4, MYB, WRKY). The expression levels of seven POD genes were up-regulated by Al stress for at least one time point in KF. The H2O2 pretreatment significantly improved Al tolerance of KF, which is likely due to increased POD activity induced by H2O2. Our results suggest that PODs play important roles in soybean tolerance to Al toxicity. We also propose a list of candidate genes for Al tolerance in KF, which would provide valuable insights into the Al tolerance mechanisms in soybean.

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