Enhanced Aluminum Tolerance in Sugarcane: Evaluation of SbMATE Overexpression and Genome-wide Identification of ALMTs in Saccharum Spp.

Abstract BackgroundA major limiting factor for plant growth is the aluminum (Al) toxicity in acidic soils, especially in tropical regions. The exclusion of Al from the root apex through root exudation of organic acids such as malate and citrate are both the most ubiquitous tolerance mechanisms in the plant kingdom. Two families of anion channels that confer Al tolerance are well described in the literature, ALMT and MATE family. ResultsIn this study, sugarcane plants constitutively overexpressing the Sorghum bicolor MATE gene (SbMATE) showed improved tolerance to Al when compared to non-transgenic (NT) plants, characterized by sustained root growth and exclusion of aluminum from the root apex based on the result obtained with hematoxylin staining. In addition, genome-wide analysis of the recently released sugarcane genome identified 11 ALMT genes and molecular studies showed potential new targets for aluminum tolerance. ConclusionsOur results indicate that the transgenic plants overexpressing the Sorghum bicolor MATE has an improved tolerance to Al. The expression profile of ALMT genes revels potential candidate genes to be used has an alternative for agricultural expansion in Brazil and other areas with aluminum toxicity in poor and acid soils.

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Aluminum tolerance in castor bean lines1

Pesquisa Agropecuária Tropical ◽

10.1590/1983-40632018v4852425 ◽

2018 ◽

Vol 48 (3) ◽

pp. 299-305

Author(s):

Lucas Barbosa de Freitas ◽

Dirceu Maximino Fernandes ◽

Suelen Cristina Mendonça Maia ◽

Laerte Gustavo Pivetta ◽

Maurício Dutra Zanotto

Keyword(s):

Castor Bean ◽

Block Design ◽

Aluminum Tolerance ◽

Acid Soils ◽

Al Tolerance ◽

Tropical Regions ◽

Randomized Block Design ◽

Bean Plants ◽

Management Techniques ◽

Adequate Management

ABSTRACT Castor bean plants are susceptible to aluminum (Al) in the soil, requiring adequate management techniques for their cultivation in acid soils containing high Al levels, as it occurs in tropical regions. This study aimed to assess the Al tolerance of castor bean lines. A randomized block design, in a 2 x 9 factorial scheme, with four replicates, was used. The treatments consisted of presence and absence of Al, as well as nine castor bean lines (CRZ H06, CRZ H11, CRZ H12, CRZ H15, CRZ H17, CRZ H18, CRZ H19, CRZ H22 and FCA). Based on a distribution into quartiles, the lines were divided into two groups. The Al-tolerant group contained the CRZ H06, H11 and H17 lines, while the group susceptible to Al was composed of CRZ H12, H15, H18, H19, H22 and FCA. The FCA and CRZ H17 lines showed the highest growth, when cultivated without Al.

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Rapid screening for aluminum tolerance in maize (Zea mays L.)

Genetics and Molecular Biology ◽

10.1590/s1415-47572000000400024 ◽

2000 ◽

Vol 23 (4) ◽

pp. 847-850 ◽

Cited By ~ 10

Author(s):

Carlos Daniel Giaveno ◽

José B. Miranda Filho

Keyword(s):

Aluminum Toxicity ◽

Aluminum Tolerance ◽

Acid Soils ◽

Maize Yield ◽

Rapid Screening ◽

Intense Staining ◽

Tropical Regions ◽

Tolerant Plants ◽

Hematoxylin Staining ◽

Two Populations

A significant decrease in maize grain yield due to aluminum toxicity is considered to be one of the most important agricultural problems for tropical regions. Genetic improvement is a useful approach to increase maize yield in acid soils, but this requires a rapid and reliable method to discriminate between genotypes. In our work we investigated the feasibility of using hematoxylin staining (HS) to detect Al-tolerant plants at the seedling stage. The original population along with two populations obtained after one cycle of divergent selection were evaluated by net root growth (NRG) and HS after 7 days in nutrient solution. Results showed a negative correlation between NRG and HS in all populations, in which sensitive plants, characterized by low NRG, exhibited more intense staining than tolerant plants. These results indicate that HS is a useful procedure for selecting Al-tolerant maize seedlings.

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Interaction between Aluminum Toxicity and Calcium Uptake at the Root Apex in Near-Isogenic Lines of Wheat (Triticum aestivum L.) Differing in Aluminum Tolerance

PLANT PHYSIOLOGY ◽

10.1104/pp.102.3.975 ◽

1993 ◽

Vol 102 (3) ◽

pp. 975-982 ◽

Cited By ~ 82

Author(s):

P. R. Ryan ◽

L. V. Kochian

Keyword(s):

Triticum Aestivum ◽

Calcium Uptake ◽

Aluminum Toxicity ◽

Aluminum Tolerance ◽

Root Apex ◽

Triticum Aestivum L ◽

Near Isogenic Lines ◽

Isogenic Lines

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CHARACTERIZATION OF DART SEQUENCES REFLECTING GENOMICREGIONS INVOLVED IN ALUMINUM TOLERANCE IN TRITICALE (X TRITICOSECALE WITTMACK)

Plant Breeding and Seed Science ◽

10.37317/pbss-2019-0005 ◽

2020 ◽

pp. 39-48

Author(s):

Agnieszka Niedziela ◽

Piotr Bednarek

Keyword(s):

Aluminum Toxicity ◽

Aluminum Tolerance ◽

Acid Soils ◽

Limiting Factor ◽

Disease Response ◽

Dna Repair Mechanisms ◽

Al Stress ◽

Blast Database ◽

Repair Mechanisms ◽

Triticosecale Wittmack

Aluminum toxicity is the major growth-limiting factor for crop cultivation on acid soils. Tolerance mecha-nisms for Al stress in triticale have not been systematically investigated so far. It is presumed, that in the case of this species they may be a function of the interaction between wheat and rye genes. In this study the se-quences of forty-six Diversity Arrays Technology markers associated with aluminum tolerance in triticale and under selection pressure were blasted against BLAST database for the identification of possible functions of the respective genome regions in Al-stress response. The analysis has showed sequences similarity to the domains involved in signaling, disease response and DNA repair mechanisms.

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Genome-Wide Identification of Soybean ABC Transporters Relate to Aluminum Toxicity

International Journal of Molecular Sciences ◽

10.3390/ijms22126556 ◽

2021 ◽

Vol 22 (12) ◽

pp. 6556

Author(s):

Junjun Huang ◽

Xiaoyu Li ◽

Xin Chen ◽

Yaru Guo ◽

Weihong Liang ◽

...

Keyword(s):

Gene Expression ◽

Abc Transporters ◽

Developmental Stages ◽

Aluminum Toxicity ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Purifying Selection ◽

Biotic Stresses ◽

Genome Wide ◽

New Germplasm

ATP-binding cassette (ABC) transporter proteins are a gene super-family in plants and play vital roles in growth, development, and response to abiotic and biotic stresses. The ABC transporters have been identified in crop plants such as rice and buckwheat, but little is known about them in soybean. Soybean is an important oil crop and is one of the five major crops in the world. In this study, 255 ABC genes that putatively encode ABC transporters were identified from soybean through bioinformatics and then categorized into eight subfamilies, including 7 ABCAs, 52 ABCBs, 48 ABCCs, 5 ABCDs, 1 ABCEs, 10 ABCFs, 111 ABCGs, and 21 ABCIs. Their phylogenetic relationships, gene structure, and gene expression profiles were characterized. Segmental duplication was the main reason for the expansion of the GmABC genes. Ka/Ks analysis suggested that intense purifying selection was accompanied by the evolution of GmABC genes. The genome-wide collinearity of soybean with other species showed that GmABCs were relatively conserved and that collinear ABCs between species may have originated from the same ancestor. Gene expression analysis of GmABCs revealed the distinct expression pattern in different tissues and diverse developmental stages. The candidate genes GmABCB23, GmABCB25, GmABCB48, GmABCB52, GmABCI1, GmABCI5, and GmABCI13 were responsive to Al toxicity. This work on the GmABC gene family provides useful information for future studies on ABC transporters in soybean and potential targets for the cultivation of new germplasm resources of aluminum-tolerant soybean.

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Genetic architecture underlying the expression of eight α-amylase trypsin inhibitors

Theoretical and Applied Genetics ◽

10.1007/s00122-021-03906-y ◽

2021 ◽

Author(s):

Khaoula EL Hassouni ◽

Malte Sielaff ◽

Valentina Curella ◽

Manjusha Neerukonda ◽

Willmar Leiser ◽

...

Keyword(s):

Genetic Architecture ◽

Intestinal Inflammation ◽

Wheat Breeding ◽

Trypsin Inhibitors ◽

Potential Candidate ◽

Lipid Transfer ◽

Wheat Cultivars ◽

Genome Wide ◽

Close Physical Proximity ◽

Major Qtl

Abstract Key message Wheat cultivars largely differ in the content and composition of ATI proteins, but heritability was quite low for six out of eight ATIs. The genetic architecture of ATI proteins is built up of few major and numerous small effect QTL. Abstract Amylase trypsin inhibitors (ATIs) are important allergens in baker’s asthma and suspected triggers of non-celiac wheat sensitivity (NCWS) inducing intestinal and extra-intestinal inflammation. As studies on the expression and genetic architecture of ATI proteins in wheat are lacking, we evaluated 149 European old and modern bread wheat cultivars grown at three different field locations for their content of eight ATI proteins. Large differences in the content and composition of ATIs in the different cultivars were identified ranging from 3.76 pmol for ATI CM2 to 80.4 pmol for ATI 0.19, with up to 2.5-fold variation in CM-type and up to sixfold variation in mono/dimeric ATIs. Generally, heritability estimates were low except for ATI 0.28 and ATI CM2. ATI protein content showed a low correlation with quality traits commonly analyzed in wheat breeding. Similarly, no trends were found regarding ATI content in wheat cultivars originating from numerous countries and decades of breeding history. Genome-wide association mapping revealed a complex genetic architecture built of many small, few medium and two major quantitative trait loci (QTL). The major QTL were located on chromosomes 3B for ATI 0.19-like and 6B for ATI 0.28, explaining 70.6 and 68.7% of the genotypic variance, respectively. Within close physical proximity to the medium and major QTL, we identified eight potential candidate genes on the wheat reference genome encoding structurally related lipid transfer proteins. Consequently, selection and breeding of wheat cultivars with low ATI protein amounts appear difficult requiring other strategies to reduce ATI content in wheat products.

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Genome-wide association study and gene network analyses reveal potential candidate genes for high night temperature tolerance in rice

Scientific Reports ◽

10.1038/s41598-021-85921-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Raju Bheemanahalli ◽

Montana Knight ◽

Cherryl Quinones ◽

Colleen J. Doherty ◽

S. V. Krishna Jagadish

Keyword(s):

Grain Size ◽

Candidate Genes ◽

Gene Network ◽

Genome Wide Association ◽

Potential Candidate ◽

Genetic Loci ◽

Stay Green ◽

Yield And Quality ◽

Genome Wide ◽

Wide Range

AbstractHigh night temperatures (HNT) are shown to significantly reduce rice (Oryza sativa L.) yield and quality. A better understanding of the genetic architecture of HNT tolerance will help rice breeders to develop varieties adapted to future warmer climates. In this study, a diverse indica rice panel displayed a wide range of phenotypic variability in yield and quality traits under control night (24 °C) and higher night (29 °C) temperatures. Genome-wide association analysis revealed 38 genetic loci associated across treatments (18 for control and 20 for HNT). Nineteen loci were detected with the relative changes in the traits between control and HNT. Positive phenotypic correlations and co-located genetic loci with previously cloned grain size genes revealed common genetic regulation between control and HNT, particularly grain size. Network-based predictive models prioritized 20 causal genes at the genetic loci based on known gene/s expression under HNT in rice. Our study provides important insights for future candidate gene validation and molecular marker development to enhance HNT tolerance in rice. Integrated physiological, genomic, and gene network-informed approaches indicate that the candidate genes for stay-green trait may be relevant to minimizing HNT-induced yield and quality losses during grain filling in rice by optimizing source-sink relationships.

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