Preliminary Studies on Citotoxicity and Genotoxicity Assessment of the PMMA-TiO2 Nanocompozites for Stereolithographic Complete Dentures Manufacturing

2018 ◽  
Vol 69 (5) ◽  
pp. 1160-1165 ◽  
Author(s):  
Eugenia Eftimie Totu ◽  
Corina Marilena Cristache ◽  
Ibrahim Isildak ◽  
Ridvan Yildirim ◽  
Mihai Burlibasa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Additive Manufacturing ◽  
3D Printing ◽  
Methyl Methacrylate ◽  
Inhibitory Action ◽  
Bacterial Species ◽  
Complete Dentures ◽  
Cad Cam ◽  
Good Biocompatibility

The aim of the present study was to characterize and preliminary assess the cytotoxicity and genotoxicity of different PMMA-TiO2 nanocomposites suitable to be used for two-parts stereolithographic complete denture manufacturing. In order to improve mechanical and antibacterial characteristics of the materials for 3D printing dentures, two matrix materials consisting in commercially available solutions of poly(methyl methacrylate) (PMMA), referred as dent-PMMA respectively base-PMMA, have been doped with different amount of TiO2 nanoparticles: 0.4, 0.6, 1.0, and 4.0% by weight. The obtained nanocomposites were structural characterized and assessed for antibacterial activity, cytotoxicity and genotoxicity. The newly obtained nanocomposites presented a good inhibitory action against the considered bacterial species-Staphylococcus aureus, good biocompatibility under 4.0% by weight. The results of the preliminary studies on cytotoxicity and genotoxicity assessment recommends the use of 0.4% TiO2 dent-PMMA and base-PMMA for CAD/CAM additive manufacturing of two-parts complete dentures/overdentures.

scholarly journals Evaluation of Antibacterial Activity of Some Traditionally Used Medicinal Plants against Human Pathogenic Bacteria

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Bishnu P. Marasini ◽  
Pankaj Baral ◽  
Pratibha Aryal ◽  
Kashi R. Ghimire ◽  
Sanjiv Neupane ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Inhibitory Action ◽  
Pathogenic Bacteria ◽  
Cynodon Dactylon ◽  
Bacterial Species ◽  
Dilution Method ◽  
Chloroform Fraction ◽  
Ethanolic Extracts

The worldwide increase of multidrug resistance in both community- and health-care associated bacterial infections has impaired the current antimicrobial therapy, warranting the search for other alternatives. We aimed to find thein vitroantibacterial activity of ethanolic extracts of 16 different traditionally used medicinal plants of Nepal against 13 clinical and 2 reference bacterial species using microbroth dilution method. The evaluated plants species were found to exert a range ofin vitrogrowth inhibitory action against the tested bacterial species, andCynodon dactylonwas found to exhibit moderate inhibitory action against 13 bacterial species including methicillin-resistantStaphylococcus aureus, imipenem-resistantPseudomonas aeruginosa, multidrug-resistantSalmonella typhi, andS. typhimurium. The minimum inhibitory concentration (MIC) values of tested ethanolic extracts were found from 31 to >25,000 μg/mL. Notably, ethanolic extracts ofCinnamomum camphora, Curculigo orchioides, andCurcuma longaexhibited the highest antibacterial activity againstS. pyogeneswith a MIC of 49, 49, and 195 μg/mL, respectively; whereas chloroform fraction ofCynodon dactylonexhibited best antibacterial activity againstS. aureuswith a MIC of 31 μg/mL. Among all,C. dactylon, C. camphora, C. orchioides, andC. longaplant extracts displayed a potential antibacterial activity of MIC < 100 μg/mL.

scholarly journals Antimicrobial Mechanism of pBD2 against Staphylococcus aureus

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3513 ◽  
Author(s):  
Kun Zhang ◽  
Heng Zhang ◽  
Chunyu Gao ◽  
Ruibo Chen ◽  
Chunli Li
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Morphological Changes ◽  
Bacterial Species ◽  
Resistant Bacteria ◽  
Quantitative Real Time Pcr ◽  
Bacterial Membranes ◽  
Qrt Pcr ◽  
High Antibacterial Activity ◽  
Therapeutic Substance

Antimicrobial peptides (AMPs) show high antibacterial activity against pathogens, which makes them potential new therapeutics to prevent and cure diseases. Porcine beta defensin 2 (pBD2) is a newly discovered AMP and has shown antibacterial activity against different bacterial species including multi-resistant bacteria. In this study, the functional mechanism of pBD2 antibacterial activity against Staphylococcus aureus was investigated. After S. aureus cells were incubated with different concentrations of pBD2, the morphological changes in S. aureus and locations of pBD2 were detected by electron microscopy. The differentially expressed genes (DEGs) were also analyzed. The results showed that the bacterial membranes were broken, bulging, and perforated after treatment with pBD2; pBD2 was mainly located on the membranes, and some entered the cytoplasm. Furthermore, 31 DEGs were detected and confirmed by quantitative real-time PCR (qRT-PCR). The known functional DEGs were associated with transmembrane transport, transport of inheritable information, and other metabolic processes. Our data suggest that pBD2 might have multiple modes of action, and the main mechanism by which pBD2 kills S. aureus is the destruction of the membrane and interaction with DNA. The results imply that pBD2 is an effective bactericide for S. aureus, and deserves further study as a new therapeutic substance against S. aureus.

scholarly journals Pharmacological Role of Anions (Sulphate, Nitrate, Oxalate and Acetate) on the Antibacterial Activity of Cobalt(II), Copper(II) and Nickel(II) Complexes With Nicotinoylhydrazine-Derived ONO, NNO and SNO Ligands

1996 ◽  
Vol 3 (5) ◽  
pp. 211-217 ◽  
Author(s):  
Zahid Hussain Chohan ◽  
Abdur Rauf
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Metal Ion ◽  
Bacterial Species ◽  
Biologically Active ◽  
Schiff Base Ligands ◽  
Mixed Ligands ◽  
And Staphylococcus Aureus

Mixed ligands biologically active complexes of cobalt(II), copper(II) and nickel(II) with nicotinoylhydrazine-derived ONO, NNO and SNO donor schiff-base ligands having the same metal ion but different anions such as sulphate, nitrate, oxalate and acetate have been synthesised and characterised on the basis of their physical, analytical and spectral data. In order to evaluate the role of anions on their bioability, these ligands and their synthesised metal complexes with various anions have been screened against bacterial species such as Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus and the title studies have proved a definative role of anions in increasing the biological activity

Mycoses, bacterial infections and antibacterial activity in sandifies (Psychodidae) and their possible role in the transmission of leishmaniasis

Parasitology ◽  
1985 ◽  
Vol 90 (1) ◽  
pp. 57-66 ◽  
Author(s):  
Y. Schlein ◽  
I. Polacheck ◽  
B. Yuval
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Leishmania Major ◽  
Bacterial Species ◽  
Jordan Valley ◽  
High Incidence ◽  
Group A

High incidence of mycoses were found in the guts and malpighian tubes of Phlebokomus papatasi from the Jordan Valley and P. tobbi from Zakinthos, Greece. Infections with several different bacteria were also found in the guts of female P. tobbi. Fungi cultured from guts of laboratory reared P. papatasi that had similar mycoses were identified as Aspergillus scierotiorum and Saccharomyces cerevisiae. Fungi-infected laboratory reared P. papatasi were refractory to artificial infections with a Leishmania major strain specific to them. The crop contents of P. papatasi, where sugar meals are stored, demonstrated antibacterial activity against the following bacterial species in culture: Escherichia coli, Staphylococcus aureus, Shigella sonnei, Streptococcus group A and Pseudomonas aeruginosa. It is postulated that the bacteria-free gut normal to sandflies is effected by the bacterial inhibitor present in the crop.

scholarly journals Studies on Some Biologically Cobalt(II), Copper(II) and Zinc(II) Complexes With ONO, NNO and SNO Donor Pyrazinoylhydrazine-Derived Ligands

1998 ◽  
Vol 5 (5) ◽  
pp. 267-274 ◽  
Author(s):  
Zahid H. Chohan ◽  
Marapaka Praveen ◽  
Syed K. A. Sherazi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacterial Species ◽  
Analytical Data ◽  
Biologically Active ◽  
Activity Data ◽  
Distorted Octahedral ◽  
Physicochemical Studies

Biologically active complexes of Co(II), Ni(II), Cu(II) and Zn(II) with novel ONO, NNO and SNO donor pyrazinoylhydrazine-derived compounds have been prepared and characterized on the basis of analytical data and various physicochemical studies. Distorted octahedral structures for all the complexes have been proposed. The synthesized ligands and their complexes have been screened for their antibacterial activity against bacterial species Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumonae. The activity data show the metal complexes to be more active than the parent free ligands against one or more bacterial species.

scholarly journals Ιστολογική ανάπτυξη εξατομικευμένου χόνδρινου εμφυτεύματος από ανθρώπινα μεσεγχυματικά βλαστικά κύτταρα λιπώδους ιστού, σε τρισδιάστατο εκτυπωμένο ικρίωμα με χρήση προηγμένων μεθόδων κατασκευής CAD/CAM και βιοαντιδραστήρα

2020 ◽  
Author(s):  
Κωνσταντίνος Θεοδωρίδης
Keyword(s):  
Additive Manufacturing ◽  
3D Printing ◽  
Perfusion Bioreactor ◽  
Micro Ct ◽  
Cad Cam

Στην παρούσα Διατριβή χρησιμοποιήθηκε η τεχνολογία ‘προσθετικής κατασκευής’ (Additive Manufacturing) και συγκεκριμένα η μοντελοποίηση με απόθεση τήξης (FDM) και τρισδιάστατη εκτύπωση (3D printing) για την κατασκευή τρισδιάστατων τυπωμένων ικριωμάτων πολυκαπρολακτόνης διαφορετικών γεωμετρικών πόρων και μικρο-αρχιτεκτονικής δομής. Παρουσιάστηκε μια συγκριτική ανάλυση ανάπτυξης υαλοειδούς χόνδρου από διαφοροποιημένα μεσεγχυματικά κύτταρα προερχόμενα από ανθρώπινο λιπώδη ιστό (hADMSCs) σε τρία διαφορετικά και πρωτότυπα ικριώματα στην εσωτερική δομή τους. Αξιολογήθηκε ο πολλαπλασιασμός των κυττάρων, ο εποικισμός και η διαφοροποίησή τους, σε σχέση με τη δομή του κάθε ικριώματος, καθώς και οι μηχανικές ιδιότητες των τελικών κατασκευών. Ιδιαίτερη έμφαση δόθηκε τόσο στην μάκρο- όσο και στην μίκρο-αρχιτεκτονική, για την βέλτιστη χονδρογενή διαφοροποίηση, ως μία σημαντική παράμετρος που δεν έχει μελετηθεί καλά στη βιβλιογραφία. Μεταξύ των τριών ικριωμάτων που δοκιμάστηκαν, το RO45 ήταν ευνοϊκότερο για χονδρογενή διαφοροποίηση, ενώ το 3DHC υποστήριξε καλύτερα τον πολλαπλασιασμό και την διείσδυση των κυττάρων εντός του ικριώματος, παρουσιάζοντας επίσης και τον υψηλότερο ρυθμό αύξησης στις μηχανικές ιδιότητες του τελικού αναγεννημένου χόνδρινου ιστού. Προσδιορίστηκε επίσης το πιο ευνοϊκό περιβάλλον για την ιδανικότερη υποστήριξη του πολλαπλασιασμού και της διαφοροποίησης των κυττάρων. Για το σκοπό αυτό, χρησιμοποιήθηκε βιοαντιδραστήρας διάχυσης (perfusion bioreactor) που προσελκύει συνεχώς την προσοχή των ερευνητών ως ένα αποτελεσματικό και σύγχρονο εργαλείο στη μηχανική ιστών. Επίσης εφαρμόστηκε ένα φυσικό βιοϋλικό ευρέως διαδεδομένο στην Αναγεννητική Ιατρική, με εξαιρετική βιοσυμβατότητα, βιοαποικοδομησιμότητα και μη τοξικά χαρακτηριστικά. Πρόκειται για το κολλαγόνο, μια δομική πρωτεΐνη με αδιαμφισβήτητα ευεργετικά χαρακτηριστικά. Η μελέτη εκτελέστηκε σε τρισδιάστατα εκτυπωμένα ικριώματα πολυκαπρολακτόνης που παρασκευάστηκαν με τετράγωνους πόρους. Το κολλαγόνο είτε εφαρμόστηκε μεμονωμένα και επικάλυψε τις ίνες του ικριώματος, είτε εφαρμόστηκε ως ένα διάλυμα γέλης κολλαγόνου και κύτταρων που συνολικά διείσδυσε στους πόρους των ικριωμάτων. Χρησιμοποιήθηκαν επιπλέον δύο διαφορετικά μέσα διαφοροποίησης για τον έλεγχο της χονδρογενούς διαφοροποίησης σε όλα τα ικριώματα, σε συνθήκες στατικής και δυναμικής καλλιέργειας με βιοαντιδραστήρες διάχυσης. Στη δυναμική καλλιέργεια, τα κύτταρα στα ικριώματα χωρίς την προσθήκη κολλαγόνου είχαν καλύτερη διείσδυση και πιο ομοιόμορφη κατανομή εντός του ικριώματος. Αυτά δε που συνδυάστηκαν με το μέσο χονδρογενούς διαφοροποίησης βασισμένο στον αυξητικό παράγοντα TGF-β2, έδειξαν επαυξημένη χονδρογενή διαφοροποίηση που οφείλονταν στο ευεργετικό μηχανικό ερέθισμα της δυναμικής καλλιέργειας. Περαιτέρω, μελετήθηκε η αρχική προσκόλληση των κυττάρων στα τρισδιάστατα ικριώματα, για να επιτευχθεί υψηλή πυκνότητα κυττάρων και ακόμη πιο ομοιόμορφη κατανομή κυττάρων, που γενικά θεωρείται μια πρόκληση σε εφαρμογές Ιστομηχανικής. Για τον σκοπό αυτό σχεδιάστηκε, κατασκευάστηκε και μελετήθηκε μια πρωτότυπη συσκευή τρισδιάστατης καλλιέργειας κυττάρων (3D-CD) για στατική καλλιέργεια με γνώμονα τον περιορισμό απώλειας κυττάρων κατά την έγχυση και την διευκόλυνση παροχής θρεπτικών ουσιών. Η τεχνολογία τρισδιάστατης εκτύπωσης (3D printing) χρησιμοποιήθηκε τόσο για το ικρίωμα όσο και για την κατασκευή της συσκευής καλλιέργειας. Εκτός από τη δοκιμή της συσκευής, ο σκοπός αυτής της μελέτης ήταν να εκτιμηθούν και να συγκριθούν οι στατικές και δυναμικές μέθοδοι έγχυσης και οι συνθήκες καλλιέργειας των κυττάρων, υπό κανονικές και υποξικές συνθήκες (normoxia and hypoxia) και τα αποτελέσματά τους σε παραμέτρους όπως η αποτελεσματικότητα διασποράς των κυττάρων, η κατανομή των κυττάρων και ο πολλαπλασιασμός των κυττάρων. Η συσκευή καλλιέργειας (3D-CD) βελτίωσε την αποτελεσματικότητα έγχυσης των κυττάρων με σχεδόν μηδαμινή απώλεια κυττάρων και με ομοιόμορφο εποικισμό σε όλες τις ζώνες του ικριώματος, όπως αναδείχθηκε από τις χρησιμοποιούμενες τεχνικές σάρωσης με μικροτομογράφο (micro-CT). Σε επόμενη φάση αξιολογήθηκε η περαιτέρω διαφοροποίηση των μεσεγχυματικών κυττάρων με αυτή την συσκευή και σε συνθήκες νορμοξίας ή υποξίας. Η μέθοδος διαφοροποίησης στην πρωτότυπη συσκευή σε συνδυασμό με τις συνθήκες υποξίας είχε ως αποτέλεσμα την αναγέννηση ιστού χόνδρου με ιδανικά χαρακτηριστικά όπως άφθονη εξωκυττάρια θεμέλια ουσία (ECM) πλούσια σε γλυκοζαμινογλυκάνες και πρωτεογλυκάνες, αλλά επίσης και αρκετά αυξημένη αναλογία σε κολλαγόνο τύπου ΙΙ έναντι του κολλαγόνου τύπου Ι, όπως και στο φυσιολογικό υαλοειδή χόνδρο. Τέλος, αξίζει να σημειωθεί ότι οι μηχανικές ιδιότητες που προέκυψαν μπορούν να αντέξουν ακόμη και τις πιο ακραίες συνθήκες της καθημερινής καταπόνησης των αρθρώσεων.

scholarly journals Rapid Antibacterial Activity of Cannabichromenic Acid against Methicillin-Resistant Staphylococcus aureus

Antibiotics ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 523
Author(s):  
Maria Galletta ◽  
Tristan A. Reekie ◽  
Gayathri Nagalingam ◽  
Amy L. Bottomley ◽  
Elizabeth J. Harry ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bactericidal Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Lipid Membrane ◽  
Bacterial Species ◽  
High Dose ◽  
Methicillin Resistant ◽  
Microscopic Evaluation ◽  
Bacterial Lipid

Methicillin-resistant Staphylococcus aureus (MRSA) has proven to be an imminent threat to public health, intensifying the need for novel therapeutics. Previous evidence suggests that cannabinoids harbour potent antibacterial activity. In this study, a group of previously inaccessible phytocannabinoids and synthetic analogues were examined for potential antibacterial activity. The minimum inhibitory concentrations and dynamics of bacterial inhibition, determined through resazurin reduction and time-kill assays, revealed the potent antibacterial activity of the phytocannabinoids against gram-positive antibiotic-resistant bacterial species, including MRSA. One phytocannabinoid, cannabichromenic acid (CBCA), demonstrated faster and more potent bactericidal activity than vancomycin, the currently recommended antibiotic for the treatment of MRSA infections. Such bactericidal activity was sustained against low-and high-dose inoculums as well as exponential- and stationary-phase MRSA cells. Further, mammalian cell viability was maintained in the presence of CBCA. Finally, microscopic evaluation suggests that CBCA may function through the degradation of the bacterial lipid membrane and alteration of the bacterial nucleoid. The results of the current study provide encouraging evidence that cannabinoids may serve as a previously unrecognised resource for the generation of novel antibiotics active against MRSA.

scholarly journals Synthesis of Mixed Ligand Ruthenium (II/III) Complexes and Their Antibacterial Evaluation on Drug-Resistant Bacterial Organisms

2020 ◽  
Vol 2020 ◽  
pp. 1-10 ◽  
Author(s):  
James T. P. Matshwele ◽  
Florence Nareetsile ◽  
Daphne Mapolelo ◽  
Pearl Matshameko ◽  
Melvin Leteane ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Magnetic Susceptibility ◽  
Klebsiella Pneumoniae ◽  
Bacterial Species ◽  
Mixed Ligand ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Bacterial Dna ◽  
Polypyridyl Ligand

The potential antimicrobial properties of a tridentate polypyridyl ligand 4-butoxy-N,N-bis(pyridin-2-ylmethyl)aniline (BUT) 1 and its corresponding mixed ligand ruthenium complexes were investigated on drug-resistant and non-drug-resistant bacterial species. The ligand and its complexes were synthesized and successfully characterized by 1H NMR, UV/Vis, and FTIR spectra; ESI-MS; and magnetic susceptibility. Electronic spectra and magnetic susceptibility of these Ru(II)/(III) complexes suggest that they are of a low spin crystal field split, where the Ru(III) is a d5 and Ru(II) d6 low spin. These compounds were tested for antibacterial activity on two bacterial species: Staphylococcus aureus (S. aureus) and Klebsiella pneumoniae (K. pneumoniae), as well as their drug-resistant strains methicillin-resistant Staphylococcus aureus (MRSA) and multidrug resistant Klebsiella pneumoniae (MDR K. pneumoniae). All the compounds inhibited growth of the two non-drug-resistant bacteria and only one drug-resistant strain MRSA. However, only the ligands BUT and 2,2-dipyridylamine showed activity against MRSA, while all complexes did not show any antibacterial activity on MRSA. We observed large zones of inhibition for the Gram-positive S. aureus and MRSA bacteria, compared to the Gram-negative K. pneumoniae bacteria. DNA cleavage studies with gel electrophoresis showed denatured bacterial DNA on the gel from all the complexes, with the exception of the ligand, suggesting DNA nuclease activity of the complexes in the bacterial DNA.

scholarly journals In VitroAntibacterial and Antifungal Activity of Salicylanilide Benzoates

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Martin Krátký ◽  
Jarmila Vinšová ◽  
Vladimír Buchta
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Benzoic Acid ◽  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Phenyl Benzoate ◽  
Methicillin Resistant ◽  
Micromolar Range

The resistance to antimicrobial agents brings a need of novel antimicrobial agents. We have synthesized and found thein vitroantibacterial activity of salicylanilide esters with benzoic acid (2-(phenylcarbamoyl)phenyl benzoates) in micromolar range. They were evaluatedin vitrofor the activity against eight fungal and eight bacterial species. All derivatives showed a significant antibacterial activity against Gram-positive strains with minimum inhibitory concentrations ≥0.98 μmol/L including methicillin-resistantStaphylococcus aureusstrain. The most active compounds were 5-chloro-2-(3,4-dichlorophenylcarbamoyl)phenyl benzoate and 4-chloro-2-(4-(trifluoromethyl)phenylcarbamoyl)phenyl benzoate. The antifungal activity is significantly lower.

scholarly journals Antibacterial Activity of Pakistani Honey

2019 ◽  
Vol 62 (2) ◽  
pp. 97-100
Author(s):  
Taif Shah ◽  
Niyaz Ali ◽  
Zahir Shah ◽  
Azam Hayat
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Apis Mellifera ◽  
Bacterial Species ◽  
Salmonella Typhi ◽  
Stingless Bee ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Khyber Pakhtunkhwa Province

The current study was conducted to determine the antibacterial activity of 50 crude and processed honey samples produced by Apis mellifera and stingless bee. All the honey samples were collected from different places of Districts Dir, Swat and Oghi of Khyber Pakhtunkhwa Province, Pakistan and were tested against the six ATCC bacterial species including E. coli ATCC number 25922, Pseudomonas aeruginosa ATCC number 27853, Staphylococcus aureus ATCC number 6538, Enterococcus faecalis ATCC number 19433, Salmonella typhi ATCC number 19943 and Klebsiella pneumoniae ATCC number 27736. The honey samples showed variable zones of inhibition by using Agar well plate technique. E. coli showed 17-23 mm, S. typhi 31-37 mm, E. faecalis 28 mm, P. aeruginosa 14-15 mm, K. pneumoniae 20-24 mm and Staph. aureus 19-25 mm. Most of the honey samples used in this study showed broad spectrum antibacterial activity.

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