Evaluation of Bone Healing of Artificial Defects in Laboratory Animals After Covering with Alloplastic Material

2018 ◽  
Vol 69 (7) ◽  
pp. 1728-1732
Author(s):  
Manuela Chivu ◽  
Cristian Tantar ◽  
Emilian Hutu ◽  
Raluca Monica Comaneanu ◽  
Elena Rusu
Keyword(s):  
Bone Repair ◽  
Healing Process ◽  
Bone Defects ◽  
Parietal Bone ◽  
Laboratory Animals ◽  
Alloplastic Material ◽  
Alloplastic Materials ◽  
Group I ◽  
Left Parietal Bone ◽  
The Right

Bone defects are commonly seen in clinical practice. They are caused by different types of trauma, infections, congenital malformations and cancers. Current approaches to skeletal reconstructive surgery use biomaterials, autografts or allografts. The aim of this study was to analyze bone repair from histologic point of view. To study the repair of bone defects, we used two batches of Wistar mices (Lat Rattus Norvegicus). The 46 subjects under study were divided into two equal lots. In all subjects, a round defect with a diameter of 5 mm was surgically performed on the right and left parietal bone. In the 23 subjects in group I the defect in the left parietal bone was covered with alloplastic material (Osteoset) and the defect in the right parietal bone was not covered with osteoconductive, osteoinductive or osteogenic materials. Regarding subjects in group study II, none of the surgically created bone defects were covered with alloplastic materials. Euthanasia of the subjects included in the study was performed at 2 and 4 months respectively, at the time of surgery. Euthanasia, bone sampling and assembly for microscopic preparations were done on the same day. The histological analysis of a bone repair shows the direct correlation between the healing process and the addition of alloplastic materials (Osteoset).

scholarly journals Preliminary in Vivo Evaluation of Bone Healing in Critical Size Bone Defects Implanted with an Injectable Calcium Phosphate Bone Cement (Osteopaste)

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Che Nor Zarida Che Seman ◽  
Zamzuri Zakaria ◽  
Zunariah Buyong ◽  
Mohd Shukrimi Awang ◽  
Ahmad Razali Md Ralib @ Md Raghib
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Bone Tissue ◽  
Bone Repair ◽  
Critical Size ◽  
Healing Process ◽  
Bone Defects ◽  
Calcium Phosphate Bone Cement ◽  
Rabbit Tibia

Introduction: A novel injectable calcium phosphate bone cement (osteopaste) has been developed. Its potential application in orthopaedics as a filler of bone defects has been studied. The biomaterial was composed of tetra-calcium phosphate (TTCP) and tricalcium phosphate (TCP) powder. The aim of the present study was to evaluate the healing process of osteopaste in rabbit tibia. Materials and method: The implantation procedure was carried out on thirty-nine of New Zealand white rabbits. The in vivo bone formation was investigated by either implanting the Osteopaste, Jectos or MIIG – X3 into a critical size defect (CSD) model in the proximal tibial metaphysis. CSD without treatment served as negative control. After 1 day, 6 and 12 weeks, the rabbits were euthanized, the bone were harvested and subjected for analysis. Results: Radiological images and histological sections revealed integration of implants with bone tissue with no signs of graft rejection. There was direct contact between osteopaste material and host bone. The new bone was seen bridging the defect. Conclusion: The result showed that Osteopaste could be a new promising biomaterial for bone repair and has a potential in bone tissue engineering.

scholarly journals Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs

2020 ◽  
Vol 8 (1) ◽  
pp. 7 ◽  
Author(s):  
Savvas Titsinides ◽  
Theodore Karatzas ◽  
Despoina Perrea ◽  
Efstathios Eleftheriadis ◽  
Leonidas Podaropoulos ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Repair ◽  
Healing Process ◽  
Bone Substitutes ◽  
Bone Defects ◽  
New Bone Formation ◽  
Post Surgery ◽  
Beta Tricalcium Phosphate ◽  
Close Proximity ◽  
Tissue Specimens

Regeneration of large jaw bone defects still remains a clinical challenge. To avoid incomplete bone repair, bone grafts have been advocated to support the healing process. This study comparatively evaluated new bone formation among a synthetic graft substitute, a human bone derivative, and a bovine xenograft. Materials were placed in 3 out of the 4 bone cavities, while 1 deficit was left empty, serving as a control, in mono-cortical defects, surgically prepared in the porcine calvaria bone. Animals were randomized in 2 groups and euthanized at 8 and 12 weeks. Harvested tissue specimens were qualitatively evaluated by histology. New bone formation was quantitatively measured by histomorphometry. Maximum new bone formation was noticed in defects grafted with beta-tricalcium phosphate b-TCP compared to the other bone substitutes, at 8 and 12 weeks post-surgery. Bovine and human allograft induced less new bone formation compared to empty bone cavity. Histologic analysis revealed that b-TCP was absorbed and substituted significantly, while bovine and human allograft was maintained almost intact in close proximity with new bone. Based on our findings, higher new bone formation was detected in defects filled with b-TCP when compared to bovine and human graft substitutes.

Chronic spontaneous diploic hematoma

2011 ◽  
Vol 114 (6) ◽  
pp. 1812-1817 ◽  
Author(s):  
José González-Tortosa ◽  
Javier Ros de San Pedro ◽  
Guillermo Parrilla ◽  
Belen Ferri-Ñiguez ◽  
Juan F. Martínez-Lage
Keyword(s):  
Osteolytic Lesion ◽  
Venous Drainage ◽  
Neurofibromatosis Type ◽  
Parietal Bone ◽  
Café Au Lait Spots ◽  
Skull Radiography ◽  
Left Parietal Bone ◽  
Cortical Venous Drainage ◽  
The Right

The authors report the case of a 23-year-old woman with café-au-lait spots and axillary and inguinal freckling who presented with a diploic chronic spontaneous hematoma of the left parietal bone. To the authors' knowledge, this case represents the first description of a diploic hematoma in a patient with stigmata of neurofibromatosis Type 1 unrelated to head trauma. Plain skull radiography showed an osteolytic lesion with well-circumscribed margins, corresponding to the hematoma, together with exuberant perilesional vascular markings. Angiography demonstrated an incidental aneurysm of the left supraclinoidal internal carotid artery and an unusual cortical venous drainage toward the diploic vessels. The blood flow of these vessels on the right hemicranium was sluggish and exhibited enlarged diploic venous lacunas. The authors hypothesize that the hematoma was formed by both an abnormal venous drainage toward the diploic vascular net, together with a vasculopathy that caused stenosis and obstruction of the normal drainage pathways from these vessels.

Evaluation of the Long-Term Results of Rat Cranial Bone Repair Using a Particular Xenograft

2010 ◽  
Vol 36 (3) ◽  
pp. 167-173 ◽  
Author(s):  
Hakan Develioglu ◽  
SerpilÜnver Saraydın ◽  
Ünal Kartal ◽  
Levent Taner
Keyword(s):  
Bone Formation ◽  
Bone Repair ◽  
Fibrous Tissue ◽  
Bone Defects ◽  
Parietal Bone ◽  
Control Area ◽  
Cranial Bone ◽  
Long Term Results ◽  
Tissue Samples ◽  
Defect Sites

Abstract Bone defects that cannot be healed completely are termed critical-sized defects and can be used to test bone grafts for medicine, dentistry, and periodontology. The aim of the present study was to detect the effects of a xenograft (Unilab Surgibone) on bone building in experimentally created parietal bone defects in rats. Standardized parietal bone defects were created in 16 rats, and each defect had a circular morphology 6 mm in diameter. The right defect sites were filled with porous particle material, and the left site was used as control. After the 3rd, 6th, and 12th months, rats were killed and tissue samples obtained from the related site of the cranium. Subsequently, histological sections were taken and stained with different stains for evaluation under light microscope. The rate of bone formation was assessed using a semiquantitative method. These results showed that dense collagenous tissue was observed in the control area during the third month, whereas xenograft particles were surrounded by a fibrous tissue layer at the implantation site. Osteoclast-like cells were also observed. There was also no significant bone repair at other observation periods. It can be concluded that the material used had no evidence of resorption and does not enhance bone formation. However, it seems biocompatible, osteoconductive, and could be used in a limited manner as a material for filling osseous defects in clinical practice.

scholarly journals Effect of PDGF-BB, IGF-I Growth Factors and their Combination Carried by Liposomes in Tooth Socket Healing

2013 ◽  
Vol 24 (4) ◽  
pp. 299-307 ◽  
Author(s):  
Fernando Antonio Mauad de Abreu ◽  
Cynthia Lopes Ferreira ◽  
Gerluza Aparecida Borges Silva ◽  
Camila de Oliveira Paulo ◽  
Melissa Nunes Miziara ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Blood Vessels ◽  
Bone Repair ◽  
Blood Clot ◽  
Healing Process ◽  
Igf I ◽  
Bone Trabeculae ◽  
The Right ◽  
Endothelial Growth Factor

This work evaluated the bone-forming potential of the platelet-derived growth factor isoform BB (PDGF-BB), insulin-like growth factor I (IGF-I), and mixed PDGF-BB/IGF-I delivered in liposomes compared with phosphate buffered saline (PBS), in the healing process of rat tooth sockets. One hundred and twelve Wistar rats were randomized into 7 groups of 16 animals each and were evaluated at 3, 7, 14 and 21 days after extraction of the maxillary second molars. The left sockets were treated with PBS (P), empty liposome (L), IGF-I in PBS (IP), IGF-I in liposome (IL), PDGF-BB in PBS (PDP), PDGF-BB in liposome (PDL) and both growth factors (GFs) together within liposomes (PDIL). The right sockets were filled with blood clot (BC). Histological and histomorphometric analyses were used to evaluate the formation of new bone and blood vessels. Immunohistochemistry was performed to evaluate the expression of osteocalcin and vascular endothelial growth factor (VEGF) during bone repair. Data were tested statistically using a Tukey's test according to a Dunn's analysis and Mann-Whitney U test followed by Kruskal-Wallis one-way analysis. Results were considered significant when p<0.05. A significantly higher percentage of bone trabeculae and a higher number of blood vessels were observed in the IL, PDL and PDIL groups (p<0.05). However, these GF-liposome groups had statistically similar results. Immunohistochemical assays first detected osteocalcin and VEGF expression at 3 days followed by a peak at 7 days. Lower immunoreactivity levels were observed in the BC, L, P, IP and PDP groups compared with the IL, PDL and PDIL groups (p<0.05). The results suggest that GFs carried by liposomes, either in isolated or mixed forms, enhanced the healing process in rat tooth sockets. The differential expression of the osteogenic markers VEGF and osteocalcin in the early phases of bone healing support these findings.

Multiple Growths of Primary Calvarial Meningiomas

Neurosurgery ◽  
1991 ◽  
Vol 29 (3) ◽  
pp. 452-455 ◽  
Author(s):  
Naoyuki Nakao ◽  
Kenji Kubo ◽  
Hiroshi Moriwaki
Keyword(s):  
Histological Examination ◽  
Parietal Bone ◽  
Review Of The Literature ◽  
Left Parietal Bone ◽  
The Right ◽  
Cellular Pleomorphism

Abstract The authors present the case of a 68-year-old woman with multiple growths of primary calvarial meningiomas. Histological examination revealed one tumor arising in the left parietal bone that showed some malignant aspects, including hypercellularity, cellular pleomorphism, necrotic foci, and some mitoses; the tumor partially had a meningotheliomatous pattern. Two other tumors in the right parietal bone were meningotheliomatous meningiomas without any malignant features. With a review of the literature, the pathogenesis of the multiplicity is discussed.

scholarly journals Histopathological, Histomorphometrical, and Radiographical Evaluation of Injectable Glass-Ceramic-Chitosan Nanocomposite in Bone Reconstruction of Rat

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Maryam Seyedmajidi ◽  
Seyedmahmood Rabiee ◽  
Sina Haghanifar ◽  
Seyedkamal Seyedmajidi ◽  
Seyed Gholam ali Jorsaraei ◽  
...  
Keyword(s):  
Glass Ceramic ◽  
Bone Repair ◽  
Tumor Resection ◽  
Bone Defects ◽  
Bone Lesions ◽  
Bone Reconstruction ◽  
Bone Thickness ◽  
Left Tibia ◽  
Male Wistar Rats ◽  
The Right

Background. Bone defects following tumor resection and osteolysis due to bone lesions, periodontal tissue disorders, and bone reconstruction are challenges that surgeons face. Gass-ceramic-chitosan nanocomposite contains chitosan, a derivative of crustaceans’ exoskeleton.Methods. Thirty-two 6–8-week-old male Wistar rats were chosen. One hole on each right and left tibia was made. The right tibia holes were filled with injectable glass-ceramic-chitosan nanocomposite, and the left tibia holes were left empty. After 7, 14, 28, and 60 days, histopathological, histomorphometrical, and radiographical assessments were performed.Results. Radiographic density on days 7 and 14 was significantly higher in the right tibias than in the left tibias. Trabecular bone thickness, which was higher in the right tibias, increased from day 7 to day 60 in both right and left tibias, although not significantly.Conclusions. Glass-ceramic-chitosan nanocomposite is suggested for use in bone repair in cases of bone loss. More histopathological, histomorphometrical, and radiographical assessments are also recommended.

scholarly journals Bone repair after osteotomy with diamond burs and CVD ultrasonic tips – histological study in rats

2010 ◽  
Vol 10 (3) ◽  
Author(s):  
Fábio S. Matuda ◽  
Clovis Pagani ◽  
Carolina B. Miranda ◽  
Aline A. S. Crema ◽  
Aline S. Brentel ◽  
...  
Keyword(s):  
Vapor Deposition ◽  
High Speed ◽  
Bone Repair ◽  
Histological Analysis ◽  
Histological Study ◽  
Polarized Light ◽  
Chemical Vapor ◽  
Bone Defects ◽  
Water Cooling ◽  
The Right

This study histologically evaluated the behavior of bone tissue of rats submitted to osteotomy with conventional diamond burs in high speed and a new ultrasonic diamond tips system (CVD – Chemical Vapor Deposition), at different study periods. The study was conducted on 24 Wistar rats. Osteotomy was performed on the posterior paws of each rat, with utilization of diamond burs in high speed under thorough water cooling at the right paw, and CVD tips at the left paw. Animals were killed at 0, 7, 14 and 21 days after treatment, followed by removal of connective tissue and bone at the area of osteotomy. Specimens were fixated, demineralized, dehydrated and sectioned for histological analysis by polarized light microscopy (25, 100 and 200x magnification) by two examiners. The results demonstrated that there were no differences in the repair of bone defects between the two different materials employed for osteotomy.

scholarly journals Extreme bone lengthening by bone transport with a unifocal tibial corticotomy: a case report

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Hongjie Wen ◽  
Huagang Yang ◽  
Yongqing Xu
Keyword(s):  
Bone Defect ◽  
External Fixator ◽  
Bone Repair ◽  
Bone Defects ◽  
Bone Transport ◽  
External Fixators ◽  
Bone Lengthening ◽  
Fracture Nonunion ◽  
Defect Repair ◽  
The Right

Abstract Background Bone transport is used for the treatment of extensive limb bone defects. The application of ring or unilateral external fixators combined with single or double corticotomy are well documented; however, there are few cases adopting a single corticotomy to repair bone defects > 24 cm. Case presentation The present case study describes an 18-year-old male, who was involved in a traffic accident and was diagnosed with open fracture of the right tibia. The patient received emergency surgery in a local hospital and was transferred to The Second People’s Hospital of Yunnan for further treatment 3 months later. The patient was diagnosed with fracture nonunion and infection following admission. Complete debridement was performed three times to control the infection. The infection was resolved after 26 days and the 24.5 cm massive tibia defect remained the biggest challenge. The bone transport technique involving a unilateral external fixator and single corticotomy was employed to treat the bone defect. Docking site union was achieved and bone consolidation was complete 40 months after corticotomy. The external fixator was subsequently removed. The bone healing index was 1.6 months/cm. The Association for the Study and Application of the Method of Ilizarov criteria (ASAMI) revealed a good functional and bone repair result. Similarly, Knee Society Score (KSS) yielded good result and the The Lower Extremity Functional Scale (LEFS) was 65. A total of 45 months after injury, the patient was able to walk painlessly without ambulatory assistive devices and resumed daily activities successfully. Eighteen months after the bone and soft tissue wound have healed, the SF-36 score was 86, and the LEFS was 70. Conclusion To the best of the authors’ knowledge, the present study described the longest bone defect repair performed using bone transport with single level corticotomy.

LOCAL APPLICATION OF GROWTH HORMONE INDUCES INCREASED IMMUNOEXPRESSION OF IGF-I ON CRANIOFACIAL BONE DEFECTS REPAIR – A PILOT STUDY

2021 ◽  
pp. 31-35
Author(s):  
Felipe Rychuv Santos ◽  
Carmen L. Mueller Storrer ◽  
Suyany Gabriely Weiss ◽  
Leandro Kluppel ◽  
João César Zielak ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Bone Repair ◽  
Healing Process ◽  
Bone Defects ◽  
Local Effect ◽  
Control Group ◽  
Craniofacial Bone ◽  
Factor I ◽  
Collagen Group ◽  
Igf I

The aim is to evaluate the local effect of different concentrations of growth hormone (GH) on the repair of craniofacial bone defects, through histological, histomorfometric, and insulin-like growth factor I (IGF-I) immunoexpression assessments. Critical defects (5 mm) were performed in 32 Wistar rats. The animals were divided into four groups: Group C (Control); Group S (Sponge-collagen); Group GH 0.08 mL; GH 0.104 mg; Group GH 0.1mL. Local applications were performed 3 times a week until the rats were euthanized at 60 days. The data were submitted to ANOVA and Tukey's test (P < 0.05). A healing process with predominance of collagen bers and bone neoformation near the edges of the defect was observed in groups C and S. Islands of bone neoformation were observed at the center and edges of the defect in groups GH 0.08 and GH 0.1. In GH 0.1, the bone was more compact, and the defect was completely closed in some specimens. Bone neoformation was signicantly higher in the GH-treated groups. All the specimens stained positive for IGF-I, and this immunoexpression was signicantly higher in Group GH 0.1. In conclusion, locally applied GH signicantly favored bone repair in rat calvaria, and a higher dose of GH increased the immunoexpression of IGF-I.

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