Antibiotic-Resistant Uropathogenic Escherichia coli Isolated from Children with Congenital Abnormalities of the Kidneys and Urinary Tract

2020 ◽  
Vol 65 (7-8) ◽  
pp. 23-26
Author(s):  
S. Suadkia ◽  
I. V. Podoprigora ◽  
N. V. Yashina ◽  
L. E. Sarukhanova ◽  
E. G. Kravtsov
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Multiple Drug Resistance ◽  
Age Groups ◽  
Multidrug Resistant ◽  
Urine Samples ◽  
Multiple Drug ◽  
Phenotypic Analysis ◽  
Antimicrobial Drugs ◽  
Tract Infections

Uropathogenic Escherichia coli (UPEC) is a serious health problem worldwide. UPEC's multiple drug resistance combined with virulence factors is a cause of serious concern. In childhood, urinary tract infections are of particular importance, since they can occur against the background of long-term unrecognized congenital anomalies of the kidneys and urinary tract. Of the 106 UPEC clinical isolates, 63.2% of cultures were isolated from girls' urine samples and 36.8% from boys' urine samples, which corresponds to a 1.7: 1 ratio. The antibiotic resistance of the isolated UPEC cultures was assessed in relation to 12 antimicrobial drugs. Among the tested cultures, 49% were multidrug-resistant and 20.75% were found to be resistant to imipenem. Phenotypic analysis of antibiotic susceptibility spectrum of uropathogenic E.coli (n=106) indicates a high percentage of occurrence of multi-resistant UPEC strains (49%) and imipenem-resistant UPEC strains (20.75%) among children of all age groups.

Study of Multidrug Resistance Pattern among Escherichia coli isolated from patients with Urinary tract infection

2016 ◽  
Vol 9 (6) ◽  
pp. 157
Author(s):  
Soniya Goyal ◽  
Vikas Beniwal
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Urinary Tract ◽  
Bacterial Infections ◽  
Multidrug Resistant ◽  
Resistance Pattern ◽  
Male Population ◽  
Antimicrobial Drugs ◽  
E Coli ◽  
Tract Infections

Objective: Urinary tract infections (UTIs) are some of the most common bacterial infections encountered in community and cause of significant morbidity and high medical cost. Escherichia coli is the most common pathogen belongs to Enterobacteriaceae family responsible for majority of UTI infections. Antimicrobial drugs have been routinely prescribed for empirical treatment of UTIs which has led to a dramatic increase in antibiotic resistance pattern of E. coli. The aim of present study was to analyse the multidrug resistance patterns (MDR) of E. coli isolated from UTI patients.Methods: A total of 80 urine samples collected from the patients suspected of having UTI attending Maharishi Markandeshwar Institute of Medical Sciences and Research (MMIMSR), Mullana, Ambala were cultured using standard microbiological techniques. Antibiotic susceptibility testing of E.coli was done by using minimum inhibitory concentration (MIC). MIC of tetracycline, doxycycline, azithromycin, erythromycin, ciprofloxacin, levofloxacin, ampicillin, amoxicillin and amikacin was done by agar dilution method.Results: Of the total 46 isolates contributing 33 females and 13 males were confirmed as E. coli. About 51.34% of the female patients belonged to the age group 21-40 yr and 53.84% of the male population belonged to 41-80 yr were found to be more susceptible to UTI infection. All isolates confirmed as E.coli were found to be multidrug resistant. 80% of the isolates exhibited MICs higher than 1000mg/L against β-lactams. 20% of the E. coli isolates exhibited MICs higher than 1000mg/L against ciprofloxacin, amikacin and erythromycin. 23% and 95% of E. coli isolates exhibited MICs less than 128 mg/L against doxycycline and levofloxacin respectively.Conclusion: The present study revealed the decreased susceptibility of the E.coli to all drugs. E. coli resistance profile to beta lactams, quinolones, macrolides, tetracyclines and aminoglycosides were also found to be quite high in this study emphasizing the need to educate public about appropriate use of antibiotics.NA

scholarly journals Bacterial Resistance in Urinary Tract Infections: Multidrug Resistant ESBL Producing Gram Negative Uropathogens from Patients

2021 ◽  
Author(s):  
Akosua Bonsu Karikari ◽  
Courage Kosi Setsoafia Saba ◽  
David Yembilla Yamik
Keyword(s):  
Urinary Tract ◽  
Bacterial Resistance ◽  
Multiple Drug Resistance ◽  
Age Groups ◽  
Multidrug Resistant ◽  
Northern Ghana ◽  
Multiple Drug ◽  
Gram Negative ◽  
Extended Spectrum Beta Lactamase ◽  
Resistance Patterns

Urinary tract infection is one of the most common bacterial infectious diseases encountered in clinical practice. The development and spread of multidrug resistant isolates are of great global health burden; among them, extended spectrum beta-lactamase (ESBL) producing Enterobacteriaceae has been a prime concern. This topic describes the resistance patterns of eighty three (83) Gram negative uropathogens to different classes of antibiotics. Bacteria isolates were obtained from patients of all age groups who sought medical attention at a secondary and tertiary hospital in Northern Ghana. Culture and isolation methods employed were the quantitative urine culture on Cysteine Lysine Electrolyte Deficient (CLED) agar and standard biochemical tests. ESBL production was detected using the CLSI recommended phenotypic confirmatory test along with routine antibiotic susceptibility test, adopting the Kirby-Bauer disk diffusion method. Out of 83 isolates, seven (7) Gram negative uropathogens were characterized and ESBLs were detected in 32 of the isolates. Escherichia coli was the pathogen with most ESBL positive strains. Generally high and multiple drug resistance were recorded in both ESBL and non-ESBL strains to the empirical drugs, however, ESBL positive strains significantly (p = 0.000) showed greater resistance. A notable finding was the appreciable resistance exhibited by ESBL strains to last line treatment drugs that include aminoglycosides and imipenem.

scholarly journals Effectiveness of Fosfomycin for the Treatment of Multidrug-Resistant Escherichia coli Bacteremic Urinary Tract Infections

2022 ◽  
Vol 5 (1) ◽  
pp. e2137277
Author(s):  
Jesús Sojo-Dorado ◽  
Inmaculada López-Hernández ◽  
Clara Rosso-Fernandez ◽  
Isabel M. Morales ◽  
Zaira R. Palacios-Baena ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Multidrug Resistant ◽  
Tract Infections

scholarly journals Determination of nitrofurantoin and fosfomycin susceptibility among urinary Escherichia coli isolates

2020 ◽  
Vol 8 (9) ◽  
pp. 3274
Author(s):  
Rachana Kanaujia ◽  
Amit Kumar ◽  
Malay Bajpai
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Urine Samples ◽  
E Coli ◽  
Tract Infections ◽  
Therapeutic Alternatives ◽  
Micro Organisms ◽  
Staphylococcus Spp

Background: Urinary tract infections (UTIs) are one of the most common infections. For treatment of UTIs, there are limited antibiotics due to increased resistance among uropathogens. Two older antibiotics; Nitrofurantoin and Fosfomycin have become novel oral therapeutic options against uropathogens. Aim of the study was to identify UTI causing micro-organisms and evaluate in-vitro activity of nitrofurantoin and fosfomycin against most common isolated organism (E. coli).Methods: Results of urine samples culture and susceptibility testing over a period of 1 year were analysed and included in this study.Results: Micro-organisms were isolated from 568 urine samples. Most commonly isolated organism was Escherichia coli (40.50%), followed by Klebsiella spp. (20.07%) and Staphylococcus spp. (17.07%). Susceptibility of E. coli to nitrofurantoin and fosfomycin was 91.74% and 65.65% respectively. Conclusion: Good activity of nitrofurantoin and fosfomycin against E. coli indicates that these two drugs are potential therapeutic alternatives for urinary tract infections.

scholarly journals Molecular Characterization of Multidrug Resistant Uropathogenic E. Coli Isolates from Jordanian Patients

2018 ◽  
Vol 12 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Yacoub R. Nairoukh ◽  
Azmi M. Mahafzah ◽  
Amal Irshaid ◽  
Asem A. Shehabi
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Multidrug Resistant ◽  
Urine Samples ◽  
Diffusion Method ◽  
Molecular Characteristics ◽  
Biochemical Tests ◽  
E Coli ◽  
Tract Infections ◽  
St131 Clone

Background: Emergence of multi-drug resistant uropathogenic E. coli strains is an increasing problem to empirical treatment of urinary tract infections in many countries. This study investigated the magnitude of this problem in Jordan. Methods: A total of 262 E. coli isolates were recovered from urine samples of Jordanian patients which were suspected to have urinary tract infections (UTIs). All isolates were primarily identified by routine biochemical tests and tested for antimicrobial susceptibility by disc diffusion method. Fifty representative Multidrug Resistance (MDR) E. coli isolates to 3 or more antibiotic classes were tested for the presence of resistance genes of blaCTX-M- 1, 9 and 15, carbapenemase (blaIMP, blaVIM, blaNDM-1, blaOXA-48), fluoroquinolones mutated genes (parC and gyrA) and clone of ST131 type using PCR methods. Results: A total of 150/262 (57.3%) of E. coli isolates were MDR. Urine samples of hospitalized patients showed significantly more MDR isolates than outpatients. Fifty representative MDR E. coli isolates indicated the following molecular characteristics: All were positive for mutated parC gene and gyrA and for ST131 clone, and 78% were positive for genes of CTX-M-15, 76% for CTX-M-I and for 8% CTX-M-9, respectively. Additionally, all 50 MDR E. coli isolates were negative for carbapenemase genes (blaIMP, blaVIM, blaNDM-1, blaOXA-48), except of one isolate was positive for blaKPC-2 . Conclusion: This study indicates alarming high rates recovery of MDR uropathogenic E. coli from Jordanian patients associated with high rates of positive ST131 clone, fluoroquinolone resistant and important types of blaCTX-M.

Local characteristics associated with higher prevalence of ESBL-producing Escherichia coli in community-acquired urinary tract infections: an observational, cross-sectional study

2020 ◽  
Author(s):  
Stéphanie Larramendy ◽  
Aurélie Gaultier ◽  
Jean-Pascal Fournier ◽  
Jocelyne Caillon ◽  
Leïla Moret ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Agricultural Land ◽  
Cross Sectional Study ◽  
Urine Samples ◽  
Sectional Study ◽  
Cross Sectional ◽  
E Coli ◽  
Animal Farming ◽  
Tract Infections

Abstract Objectives The prevalence of ESBL-producing Escherichia coli (ESBL-E. coli) in community-acquired urinary tract infections (UTI) has been increasing worldwide since 2000, but with large geographical variations. The aim of this study was to determine whether the ESBL-E. coli rate in urine samples from individuals with community-acquired UTI was associated with the local socio-economic, environmental, agricultural and healthcare characteristics. Methods This was a cross-sectional study in western France using data on antibiotic susceptibility of E. coli isolated from urine samples of individuals with community-acquired UTI analysed in non-hospital laboratories from 2015 to 2017. The ESBL-E. coli rate was calculated for each laboratory. Data on socio-economic characteristics, human antibiotic consumption, hospital bed density, animal farming density and percentage of agricultural land and surface water were retrieved at the municipality level and aggregated by study area. Their association with ESBL-E. coli prevalence was quantified using multivariate linear regression models with a backward selection. Results From 358 291 E. coli isolates from urine samples tested in 92 laboratories, the mean ESBL-E. coli prevalence for the study period was 3.30%. In an adjusted model, the ESBL-E. coli rate was significantly (P < 0.05) and positively associated with the local percentage of people >65 years old, third-generation cephalosporin use (DDD/1000 inhabitants), number of hospital beds/km2, poultry density, pig density and percentage of agricultural land. Lower deprivation was associated with a higher ESBL-E. coli rate. Conclusions Several anthropogenic factors (primary care, hospitals and animal farming) are associated with the local ESBL-E. coli rate in community-acquired UTI. These results could contribute to improve risk management, including identification of at-risk patient groups.

scholarly journals Changing pattern of resistant pathogens causing urinary tract infections in Karachi

2013 ◽  
Vol 2 (3) ◽  
pp. 105-110
Author(s):  
H Najmul ◽  
A Tanveer
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Antimicrobial Drugs ◽  
Klebsiella Species ◽  
Amoxicillin Clavulanic Acid ◽  
Tract Infections ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

INTRODUCTION: The study under view is based under the aim to investigate the prevalence and susceptibility pattern of pathogens, causing urinary tract infections (UTIs), to antibiotics commonly used in routine medication. MATERIALS AND METHODS: Over a period of 10 months 100 isolates were collected for the determination of their susceptibility to chosen antibiotics, from a laboratory (MedPath Laboratories) in urban area of Karachi. All Gramnegative and Gram-positive urinary tract pathogens were re-identified by their morphological and biochemical characteristics and the susceptibility to seven antibiotics was determined. RESULTS: Pathogens were found as, Escherichia coli, Pseudomona spp, Klebsiella species, Enterobacter spp., and Staphylococci spp. In recent study, more than half of the Escherichia coli isolates were resistant to one or more of the all antimicrobial drugs tested. Resistance was most common to amoxicillin/clavulanic acid and ofloxacin, cefixime, followed by gentamicin. Our results indicate that Escherichia coli and Pseudomonas spp. were the most common organisms causing UTI. Other organisms involved were Enterobacter spp., Staphylococcus spp., and Klebsiella spp. Increasing patterns of resistant to gentamicin, and ofloxacin were also observed. CONCLUSIONS: In conclusion, pattern of antibiotic susceptibility to first line antibiotics is changing hence antimicrobial susceptibility testing of all isolates is crucial for the treatment of UTI. DOI: http://dx.doi.org/10.3126/ijim.v2i3.8069 Int J Infect Microbiol 2013;2(3):99-104  

scholarly journals Bacterial Resistance to Leucyl-tRNA Synthetase Inhibitor GSK2251052 Develops during Treatment of Complicated Urinary Tract Infections

2014 ◽  
Vol 59 (1) ◽  
pp. 289-298 ◽  
Author(s):  
Karen O'Dwyer ◽  
Aaron T. Spivak ◽  
Karen Ingraham ◽  
Sharon Min ◽  
David J. Holmes ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Bacterial Resistance ◽  
Multidrug Resistant ◽  
Trna Synthetase ◽  
Content Type ◽  
Development Of Resistance ◽  
Tract Infections ◽  
Abdominal Infections

ABSTRACTGSK2251052, a novel leucyl-tRNA synthetase (LeuRS) inhibitor, was in development for the treatment of infections caused by multidrug-resistant Gram-negative pathogens. In a phase II study (study LRS114688) evaluating the efficacy of GSK2251052 in complicated urinary tract infections, resistance developed very rapidly in 3 of 14 subjects enrolled, with ≥32-fold increases in the GSK2251052 MIC of the infecting pathogen being detected. A fourth subject did not exhibit the development of resistance in the baseline pathogen but posttherapy did present with a different pathogen resistant to GSK2251052. Whole-genome DNA sequencing ofEscherichia coliisolates collected longitudinally from two study LRS114688 subjects confirmed that GSK2251052 resistance was due to specific mutations, selected on the first day of therapy, in the LeuRS editing domain. Phylogenetic analysis strongly suggested that resistantEscherichia coliisolates resulted from clonal expansion of baseline susceptible strains. This resistance development likely resulted from the confluence of multiple factors, of which only some can be assessed preclinically. Our study shows the challenges of developing antibiotics and the importance of clinical studies to evaluate their effect on disease pathogenesis. (These studies have been registered at ClinicalTrials.gov under registration no. NCT01381549 for the study of complicated urinary tract infections and registration no. NCT01381562 for the study of complicated intra-abdominal infections.)

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