Effectiveness of Mixed Clove Flower Extract (Syzygium Aromaticum) And Sweet Wood (Cinnamon Burmanni) on the Growth of Enterococcus Faecalis

Abstract: Cloves (Syzygium aromaticum L.) grow widely in Indonesia. This plant has many benefits from its stem, leaves, and flowers. Clove leaves has several antibacterial compounds such as flavonoids, tannins, and triterpenoids, as well as eugenol as the major component of essential oil. Enterococcus faecalis is a facultative anaerobic Gram positive bacteria and normal flora in the mouth. These bacteria are often identified as the cause of the failure of root canal treatment. This study was aimed to determine the effectiveness of clove in inhibiting the growth of bacteria Enterococcus faecalis. This was an experimental study with a modified method of Kirby-Bauer using pits. Samples of clove leaves were obtained from Treman, North Minahasa, and then were extracted by using maceration method with 96% ethanol. Metronidazole was used as positive control. Enterococcus faecalis bacteria were taken from the direct isolation of patients’ necrotic teeth. The results showed that the average inhibition zone of clove leaf extract against Enterococuss faecalis was 8.0 mm meanwhile of metronidazole was 10.0 mm. Conclusion: Clove leaf extract had moderate inhibitory effect against the growth of Enterococcus faecalis.Keywords: clove leaves, Enterococcus faecalis, inhibition. Abstrak: Tanaman cengkih (Syzygium Aromaticum L.) banyak tumbuh di Indonesia. Tanaman ini memiliki banyak manfaat mulai dari batang, daun, dan bunga. Daun cengkih mengandung berbagai senyawa yang bersifat antibakteri seperti flavonoid, tannin, dan triterpenoid, serta senyawa eugenol yang merupakan komponen utama dalam minyak atrisi. Enterococcus faecalis ialah bakteri Gram positif fakultatif anaerob yang merupakan flora normal dalam mulut. Bakteri ini sering terisolasi sebagai penyebab kegagalan perawatan saluran akar. Penelitian ini bertujuan untuk mengetahui daya hambat daun cengkih (Syzygium Aromaticum L.) terhadap pertumbuhan bakteri Enterococcus faecalis. Jenis penelitian ialah eksperimental dengan metode modifikasi Kirby-Bauer menggunakan sumuran. Sampel daun cengkeh diperoleh dari daerah Treman Kabupaten Minahasa Utara yang kemudian diekstrasi dengan metode maserasi menggunakan etanol 96%. Sebagai kontrol positif diginakan metronidazole. Bakteri Enterococcus faecalis diambil dari isolasi langsung pada pasien dengan gigi nekrosis. Hasil penelitian ini mendapatkan rerata zona inhibisi ekstrak daun cengkih terhadap bakteri Enterococuss faecalis sebesar 8,0 mm sedangkan zona inhibisi metronidazole 13,0 mm. Simpulan: Ekstrak daun cengkih memiliki daya hambat yang tergolong sedang terhadap pertumbuhan bakteri Enterococcus faecalis.Kata kunci: daun cengkih, Enterococcus faecalis, daya hambat

Download Full-text

The effect of the thermoplastic nilon enterprises (valplast) on clove extract (syzygium aromaticum) in various concentrations on the color change

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v2i3.645 ◽

2017 ◽

Vol 2 (3) ◽

pp. 180

Author(s):

Niken Wibawaningtyas ◽

Dewi Kristiana ◽

Niken Probosari

Keyword(s):

Light Intensity ◽

Sample Size ◽

Color Change ◽

The Other ◽

Syzygium Aromaticum ◽

Test Results ◽

Color Changes ◽

Flower Extract ◽

Significant Difference ◽

One Way Anova

Objective: To investigate the effect of soaking of thermoplastic nylon (valplast) on clove flower extract (syzygium aromaticum) as a denture cleanser with different concentration 0.8%, 1%, 1.2%, 1.4%, and 1.6% to the color change of thermoplastic nylon.Material and Methods: 30 samples were divided into 6 groups. The sample size is (10x10x1) mm. The first group was immersed in sterile aquades and the other groups were immersed in clove flower extracts of 0.8%, 1%, 1.2%, 1.4%, and 1.6% for 12 days. The light intensity then measured using densitometer.Results: One way anova test results obtained value 0.174 (p>0.05) which indicates that there is no significant difference in each group.Conclusions: This study are darker discoloration of the thermoplastic nylon at a concentration of 1.6% and lower color changes occurring at a concentration of 0.8%.

Download Full-text

Effectiveness of Clove Flower Extract (Syzygium Aromaticum L.) as Analgesic on Licking Time Reaction in Male Mice with Formalin Induction

Jurnal Medik Veteriner ◽

10.20473/jmv.vol4.iss2.2021.226-230 ◽

2021 ◽

Vol 4 (2) ◽

pp. 226

Author(s):

Vina Puspitasari ◽

Retno Sri Wahjuni ◽

Amung Logam Saputro ◽

Iwan Sahrial Hamid ◽

Prima Ayu Wibawati

Keyword(s):

Statistical Analysis ◽

Effective Dose ◽

Syzygium Aromaticum ◽

Male Mice ◽

Random Design ◽

Treatment Groups ◽

Design Formula ◽

Flower Extract

This research is about testing the effectiveness of clove flower extract (Syzygium aromaticum L.) as an analgesic against the beating time caused by formalin male mice. This research is experimental with the Complete Random Design formula producing four times repetition (N = 4) and divided into five treatment groups (t = 5). Data were analyzed using ANAVA factorial test then followed by Duncan test. All tests are carried out with Product Solutions and Service Statistics (SPSS). The results of research and statistical analysis prove that clove flower extract at a dose of 1% is an effective dose compared to other treatment groups, seen from the amount of time licking male mice which is smaller than other treatment groups. It can be concluded that the administration of clove flower extract has analgesic effectiveness against the reaction of male mice induced by formalin. The concentration of clove flower extract 1% is the most effective in reducing licking time.

Download Full-text

Pengaruh Ekstrak Bunga Cengkeh (Syzygium Aromaticum) terhadap Zona Hambat Jamur Tricophyton Rubrum

Jurnal Laboratorium Khatulistiwa ◽

10.30602/jlk.v1i1.92 ◽

2017 ◽

Vol 1 (1) ◽

pp. 26

Author(s):

Etiek Nurhayati ◽

Kuswiyanto Kuswiyanto ◽

Karta Pilo

Keyword(s):

Skin Diseases ◽

Trichophyton Rubrum ◽

Sampling Technique ◽

Inhibition Zone ◽

Diffusion Method ◽

Syzygium Aromaticum ◽

Purposive Sampling ◽

Flower Extract ◽

Tinea Barbae ◽

Quasi Experimental

Abstract: Clove ﬂower (Syzygium aromaticum) is one type of traditional medicine. The active substance in clove ﬂower is used as antimicrobial and cure skin diseases caused by Trichophyton rubrum fungus such as Tinea capitis, Tinea barbae and Tinea corporis. This study was aimed to determine the effect of various concentrations of clove ﬂower extract (Syzygium aromaticum) with Trichophyton rubrum inhibition zone by the diffusion method. The method of this research was quasi-experimental. Sampling technique by purposive sampling. The sample used was n-hexane extract of clove ﬂower obtained from maceration process made in 9 concentrations and dilution was done by using dimethyl sulfoxide 15%. Each consisted of clove ﬂower extract with concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% and 90%. The inﬂuence test using disc method or diffusion method then analyzed with Linear Regression Test. Based on the result of research from 9 concentrations, it can be seen that the widest obstacle zone was 11 mm at 10% concentration whereas the widest zone of resistance was 40 mm at 90% concentration. Statistical analysis showed the results (p = 0,000 <a 0.005) so it can be concluded there was the effect of various concentrations of clove ﬂower extract (Syzygium aromaticum) to Trichophyton rubrum inhibition zone by the diffusion methodAbstrak: Bunga cengkeh (Syzygium aromaticum) merupakan salah satu jenis obat tradisional. Zat aktif dalam bunga cengkeh digunakan sebagai antimikroba dan menyembuhkan penyakit kulit yang disebabkan oleh jamur Trichophyton rubrum seperti Tinea kapitis, Tinea barbae dan Tinea korporis. Penelitian ini bertujuan mengetahui pengaruh berbagai konsentrasi ekstrak bunga cengkeh (Syzygium aromaticum) terhadap zona hambat jamur Trichophyton rubrum dengan metode difusi. Metode penelitian ini adalah eksperimental semu. Teknik pengambilan sampel dengan cara purposive sampling. Sampel yang digunakan adalah ekstrak n-heksana bunga cengkeh diperoleh dari proses maserasi yang dibuat dalam 9 konsentrasi dan dilakukan pengenceran menggunakan dimetil sulfoksida 15%. Masing-masing terdiri atas ekstrak bunga cengkeh dengan konsentrasi 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% dan 90% . Uji pengaruh menggunakan metode cakram atau metode difusi kemudian dianalisis menggunakan uji Regresi Linier. Berdasarkan hasil penelitian dari 9 konsentrasi dapat diketahui bahwa luas zona hambatan yang terendah yaitu 11 mm pada konsentrasi 10% sedangkan luas zona hambatan yang tertinggi yaitu 40 mm pada konsentrasi 90%. Analisis statistik menunjukkan hasil (p = 0,000 < a 0,005) sehingga dapat disimpulkan terdapat pengaruh berbagai konsentrasi ekstrak bunga cengkeh (Syzygium aromaticum) terhadap zona hambat jamur Trichophyton rubrum dengan metode difusi.

Download Full-text

Assessment of the antibacterial activity of chestnut (Castanea sativa) and cloves (Syzygium aromaticum) herbal extracts as an alternative to antibiotics use during post-hatching period of chicks

Science Technology and Innovation ◽

10.5604/01.3001.0014.8972 ◽

2021 ◽

Vol 11 (4) ◽

pp. 48-54

Author(s):

Rafał Kędzia ◽

Marcin Lis

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Enterococcus Faecalis ◽

Bacterial Infections ◽

Castanea Sativa ◽

Herbal Extract ◽

Control Group ◽

Syzygium Aromaticum ◽

Herbal Extracts

Bacterial infections of newly hatched chicks are the most common cause of their death in the initial period of rearing. These infections are always treated with antibiotics. The aim of the study was to investigate the antimicrobial activity of herbal extracts of chestnut (Castanea sativa) and clove (Syzygium aromaticum) against bacterial infections i.e. Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumonice in comparison to antibiotics. The results of the microbiological analyses showed that the Castanea sativa and Syzygium aromaticum extracts had a slighter antibacterial activity in comparison to antibiotics. The diameter of zone inhibition of the culture's growth of gram-negative bacteria (i.e. Escherichia coli and Klebsiella pneumoniae) and gram-positive bacteria (i.e. Staphylococcus aureus and Enterococcus faecalis) was 6-13 mm for these extracts in comparison to 15-30 mm for antibiotics. However, some bacterial strains presented full resistance to the selected antibiotics, e.g., wild strains of Enterobacteriaceae to amoxicillin or Staphylococcus aureus and Enterococcus faecalis to florfenicol, colistin, and doxycycline. In the second experiment, the effect of the herbal extract mixture added into drinking water on the growth and mortality of chicken broiler during the first rearing week was investigated. There was found that the use of herbal extracts improved the chickens’ body weight (157.4 g; P≤0.008) and decreased mortality rate (2.4%) compared to the control group (144.1 g and 3.9%, respectively) but not to the group treated with antibiotic (161.5 and 0.6% respectively; P≤0.009). In summary, the use of herbal extracts as a nutritional supplement for poultry seems to have a positive effect on weight gain of young birds, and to some extent reduce mortality in the first week of rearing.

Download Full-text

POTENCIAL MICROBIOLÓGICO DO SYZYGIUM AROMATICUM (L). SOBRE MICRORGANISMOS DE IMPORTÂNCIA NA MEDICINA VETERINÁRIA

10.51161/rems/1920 ◽

2021 ◽

Author(s):

Vanessa Arantes Pinto ◽

Gabriela Santos Alencar ◽

Adriano Favero ◽

Hugo Franciscon ◽

Fagner Luiz Da Costa Freitas

Keyword(s):

Pseudomonas Aeruginosa ◽

Enterococcus Faecalis ◽

Syzygium Aromaticum ◽

E Coli ◽

Streptococcus Uberis

Introdução: O fenômeno de resistência a antimicrobianos está se mostrando um grande problema na saúde, ameaçando tanto a população humana como animal. Devido a isso, a descoberta de novos compostos com capacidade antimicrobiana torna-se imprescindível, e os fitoterápicos têm se mostrado uma alternativa promissora na terapia complementar. O óleo essencial (OE) de Syzygium aromaticum (Cravo da Índia), apresenta potencial capacidade antibacteriana e antifúngica devido às altas concentrações de Eugenol. Objetivos: Avaliar o potencial antimicrobiano do óleo essencial de Syzygium aromaticum (L). contra microrganismos de importância na Medicina veterinária. Materiais e métodos: Foram testadas as bactérias gram positivas: S. aureus LB 25923, S. aureus B24, S. aureus NP 38, Streptococcus uberis, S. agalactiae, Enterococcus faecalis e S. epidermidis; as gram negativas: Pseudomonas aeruginosa ATCC 27853 e E. coli ATCC 25922; e os fungos: C. tropicalis e C. gattii 179. Utilizou-se a técnica de disco difusão (Kirby-bauer) aplicando 10µL de OE, e posteriormente, para os patógenos que apresentaram o halo inibitório, determinou-se, aplicando 15µL de OE, a concentração inibitória mínima (CIM), concentração bactericida mínima (CBM) e concentração fungicida mínima (CFM) através da microdiluição seriada utilizando leitor de ELISA e corante resazurina. Resultados: Todos os microrganismos apresentaram halo inibitório. Com relação aos valores de CIM e CBM para as bactérias, obteve-se, respectivamente: S. aureus LB25923 (18,75% e 18,75%), S. aureus B 24 (4,94% e 4,94%) S. aureus NP 38 (3,64% e 3,64%) Streptococcus uberis (2,60% e 3,125%) S. agalactiae (5,20 %, 5,20%), Enterococcus faecalis (5,20% e 6,25%), S. epidermidis (3,38% e 4,42%), Pseudomonas aeruginosa ATCC 27853 (2,09% e 2,09%), E. coli ATCC 25922 (10,41% e 10,41%). Os resultados de CIM e CFM obtidos para os fungos foram respectivamente: C. tropicalis (1,82% e 1,82%) e C. gattii 179 (5,27% e 5,27%). Conclusão: O óleo essencial de Syzygium aromaticum possui considerável potencial antimicrobiano “in vitro”, e dessa forma, pode ser considerado uma possível alternativa para as terapias sintéticas de tratamento das doenças infecciosas bacterianas e fúngicas encontradas na rotina clínica.

Download Full-text