scholarly journals Effectiveness of Clove Flower Extract (Syzygium Aromaticum L.) as Analgesic on Licking Time Reaction in Male Mice with Formalin Induction

2021 ◽  
Vol 4 (2) ◽  
pp. 226
Author(s):  
Vina Puspitasari ◽  
Retno Sri Wahjuni ◽  
Amung Logam Saputro ◽  
Iwan Sahrial Hamid ◽  
Prima Ayu Wibawati
Keyword(s):  
Statistical Analysis ◽  
Effective Dose ◽  
Syzygium Aromaticum ◽  
Male Mice ◽  
Random Design ◽  
Treatment Groups ◽  
Design Formula ◽  
Flower Extract

This research is about testing the effectiveness of clove flower extract (Syzygium aromaticum L.) as an analgesic against the beating time caused by formalin male mice. This research is experimental with the Complete Random Design formula producing four times repetition (N = 4) and divided into five treatment groups (t = 5). Data were analyzed using ANAVA factorial test then followed by Duncan test. All tests are carried out with Product Solutions and Service Statistics (SPSS). The results of research and statistical analysis prove that clove flower extract at a dose of 1% is an effective dose compared to other treatment groups, seen from the amount of time licking male mice  which is smaller than other treatment groups. It can be concluded that the administration of clove flower extract has analgesic effectiveness against the reaction of male mice induced by formalin. The concentration of clove flower extract 1% is the most effective in reducing licking time.

scholarly journals Atorvastatin Decreases Renal Menaquinone-4 Formation in C57BL/6 Male Mice

2019 ◽  
Vol 149 (3) ◽  
pp. 416-421 ◽  
Author(s):  
Stephanie G Harshman ◽  
M Kyla Shea ◽  
Xueyan Fu ◽  
Michael A Grusak ◽  
Donald Smith ◽  
...  
Keyword(s):  
Linear Models ◽  
Cholesterol Metabolism ◽  
Atmospheric Pressure Chemical Ionization ◽  
Control Diet ◽  
Male Mice ◽  
Atorvastatin Treatment ◽  
Treatment Groups ◽  
Pressure Chemical Ionization ◽  
Pressure Chemical ◽  
Coa Reductase

ABSTRACTBackgroundMenaquinone-4 (MK4), a vitamin K metabolite, is converted from phylloquinone through a process that requires intermediates of endogenous cholesterol production. Recent evidence suggests that MK4 is involved in kidney function.ObjectiveThe purpose of this study was to determine the effect of atorvastatin treatment on MK4 formation in young and old male mice.MethodsC57BL/6 male mice (4-mo-old and 20-mo-old) were randomly assigned to either a diet containing 300 mg atorvastatin/kg with 3 mg phylloquinone/kg or a control diet containing 3 mg phylloquinone/kg for 8 wk. During week 8, all mice received deuterium-labeled phylloquinone in the diet. Labeled and unlabeled phylloquinone and MK4 in liver, kidney, brain, and intestine were measured by atmospheric pressure chemical ionization LC/MS. 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase gene expression was quantified by reverse transcriptase-PCR. Tissue MK4 and phylloquinone concentrations were compared between atorvastatin treatment groups with use of general linear models.ResultsThere was no age-treatment interaction on MK4 tissue concentrations. In atorvastatin-treated mice, total MK4 and percentage of deuterium-labeled MK4 in kidney were both approximately 45% lower compared to values in mice not given atorvastatin (all P < 0.05). MK4 concentrations did not differ between groups in any other tissue measured.ConclusionIn male mice, atorvastatin reduced endogenous MK4 formation in the kidney, but not other organs. These observations are consistent with our hypothesis that cholesterol metabolism is involved in the generation of MK4. Further research is needed to understand potential regulatory mechanisms and the unique functions of MK4 in the kidney.

scholarly journals Effect of Boiled Water Parasite Kapok (Dendrophthoe pentandra) Leaves on Total Cholesterol Levels and Histopathology of Hepar of Hypercholesterolemic Mice

2019 ◽  
Vol 967 ◽  
pp. 51-55
Author(s):  
Andi Mu'nisa ◽  
Yusminah Hala ◽  
A. Mushawwir Taiyeb ◽  
Dwi Kesuma Sari
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Level ◽  
Total Cholesterol Level ◽  
Male Mice ◽  
Animal Group ◽  
Treatment Groups ◽  
Cholesterol Levels ◽  
Boiled Water ◽  
Paraffin Method ◽  
Determine Effect

This study aims to determine effect of boiled water parasite kapok (Dendrophthoe pentandra) leaves on the total cholesterol level and histopathology of hepar of hypercholesterolemic mice (mus musculus). This study used 20 ICR male mice divided into 4 treatment groups namely normal mice group, hypercholesterolemic mice group, mice group with boiled water kapok parasite at a dose of 400 mg/kg BW, and 800 mg/kg BW, respectivelly. The animal group was given boiled water kapok parasite after standard feeding and cholesterol feed for 2 weeks. The parameters observed in this study were total cholesterol levels, the amount of fat granules in the liver tissue of mice which were observed in the form of liver-preserved preparations made by paraffin method. The results showed that the administration of parasitic kapok (Dendrophthoe pentandra) at a dose of 400 mg/kg BW and 800 mg/kg BW had an effect on decreasing the total cholesterol level of mice and the histopathology of hepar of showed improvement after the therapy.

scholarly journals Aggressiveness and Libido of Male Mus musculus L. in Ethanol Extract of Red Ginger (Zingiber officinale Roxb. var. Rubrum)

2019 ◽  
Vol 6 (1) ◽  
pp. 8-14
Author(s):  
Nida Nurhanifah ◽  
Sutyarso Sutyarso ◽  
M. Kanedi ◽  
Hendri Busman
Keyword(s):  
Free Radicals ◽  
Ethanol Extract ◽  
Serum Testosterone ◽  
Zingiber Officinale ◽  
The Body ◽  
Male Mice ◽  
Ginger Extract ◽  
Treatment Groups ◽  
Randomized Design ◽  
Red Ginger

The residue of paraquat herbicides may cause human health problems. Paraquat causes the testosterone hormone to decline. Testosterone hormone affects aggressive and sexual behavior. It is caused by free radicals in the body. Free radicals can be non-reactive if in the body there are antioxidants. Red ginger could be used as an antioxidant because it has active phenolic compounds, such as arginine, gingerol, shogaol, zingerone, gingerdiol, and zingiber. Ginger could increase serum testosterone levels. The purpose of this research is to know the effect of red ginger ethanol extract on aggressiveness and libido of male mice induced by paraquat herbicide. This study used a Completely Randomized Design (RAL) consisting of six treatment groups with four replications each. Group P1 as control (aqua dest), P2 (paraquat at dose 20 mg/kg BM), P3, P4, P5 (paraquat with dose 20 mg/kg BM and red ginger extract at 200 mg/kg BM, 400 mg/kg BM, 600 mg/kg BM) and P6 (red ginger extract at 600mg/kg BM). Paraquat is given 2 times a week for 21 days and the red ginger extract is given daily for 35 days. The result of analysis with One-way ANOVA and continued BNT at 5% showed that giving of red ginger ethanol extract can increase aggressive behavior and libido of male mice induced paraquat herbicide.

scholarly journals EKSTRAK BUAH NANAS TERHADAP JUMLAH TOTAL LEUKOSIT DAN NEUTROFIL IKAN LELE (Clarias batrachus) YANG DIINFEKSI DENGAN Aeromonas hydrophila

2020 ◽  
Vol 10 ◽  
pp. 70-77
Author(s):  
Ratna Widyawati ◽  
Dyah Widhowati ◽  
Dhia Nadhifa
Keyword(s):  
Effective Dose ◽  
Aeromonas Hydrophila ◽  
Challenge Test ◽  
Positive Control ◽  
Clarias Batrachus ◽  
Ananas Comosus ◽  
Fruit Extract ◽  
Treatment Groups ◽  
Pineapple Fruit ◽  
One Way Anova

The present research to determine the Extract of Pineapple (Ananas comosus) on the survival of catfish infected with A. Hydrophila. The main problem of cultivation of catfish (C. batrachus) is a disease caused by the bacterium Aeromonas hydrophila.Catfish (Clarias batrachus) is a freshwater fish that has several advantages, one of which is a high nutritional content. Test animals used were catfish measuring 15-20cm with a body weight of ± 250gr. Challenge test by injecting A. hydrophila suspension with a dose of 108sel / mm3 as much as 0.1mm intramuscularly. Observations were made for 7 days after infection  which included the total number of leukocytes and neutrophils. This study was divided into 5 treatment groups with 5 replications, namely P1 (positive control), P2 (0.1%), P3 (0.3%), P4 (0.5%),  P5  (0.7%)  and soaking Pineapple Fruit Extract for 24 hours. Data analysis in this study used one way ANOVA statistical test. The results showed symptoms of catfish attacked by Aeromonas hydrophila including increased total number of leukocytes and neutrophils and ulceration in catfish. Soaking effect Pineapple fruit extract 0.5% effective dose can reduce the total number of leukocytes and 0.7% effective dose can reduce the total number of neutrophils.

scholarly journals PENGARUH PEMBERIAN D-GALAKTOSA TERHADAP PERUBAHAN BERAT BADAN MENCIT JANTAN

2020 ◽  
Vol 2 (2) ◽  
pp. 113-116
Author(s):  
Indah Permata Sari ◽  
Muhammad Ichwan ◽  
Yahwardiah Siregar
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Statistical Analysis ◽  
Oxidative Damage ◽  
The Body ◽  
Male Rats ◽  
Male Mice ◽  
Milk Products ◽  
The Brain

D-galactose is aldohexose which occurs naturally in the body, including in the brain which is found in lactose disaccharides in honey, beets and milk products. Needed in the body for lactose formation during the process of lactogenesis. Provision of large amounts of D-galactose can cause oxidative damage to various tissues and organs. The purpose was to see changes in body weight in male rats after being given a D-galactose injection. Samples using male mice aged 8 weeks with a weight of ± 30 grams, the number of samples was 6 mice swiss webster strain. intra-peritoneal injection of D-galactose is given for 6 weeks every day (150 mg / kg / bb). The results of this study indicate that the statistical analysis is significant p <0.05 (p = 0.016). The conclusion after being given injection of D-galactose for 6 weeks every day intra peritoneally was found D-galactose had an effect on the weight gain of male mice.

scholarly journals Perubahan Kualitas Sperma Mencit (Mus musculus) Yang Terpapar Asap Rokok Elektrik

2019 ◽  
Vol 3 (2) ◽  
pp. 122-128
Author(s):  
Andita Ayu Mandasari ◽  
Siti Nur Asiyah ◽  
Kurnia Lintang
Keyword(s):  
Treatment Group ◽  
Cigarette Smoke ◽  
Mus Musculus ◽  
Group Treatment ◽  
Time Lag ◽  
Cigarette Smoke Exposure ◽  
Control Group ◽  
Male Mice ◽  
Suction Device ◽  
Treatment Groups

Smoking is one of the habits that can affect human health. One type of cigarette that is currently the choice is electric cigarettes. The e-cigarette content includes nicotine, propylene glycol, flavorings and glycerin. The purpose of this study was to determine the effect of exposure to e-cigarette smoke on mice strain balb / c with the parameters of the number of spermatozoa. This study used 15 male mice which were 8-10 weeks old and weighed 25-30 grams of male mice which were divided into 3 treatment groups namely control group, treatment group one and treatment group two. In the control group no exposure to cigarette smoke was carried out while in the treatment group one was exposed to cigarette smoke in mice with a dose of 0 mg nicotine and the second treatment group was exposed to e-cigarette smoke at a dose of 18 mg nicotine. Electric cigarette smoke exposure is carried out for 30 days using a suction device from a syringe. The exposure of cigarette smoke is carried out every day with successive doses of 20 times as much as 3 times with a time lag of 10 minutes (5 minutes the chamber is closed and 5 minutes of the chamber is opened). The results showed that the average number of spermatozoa in the control group was 16.816 million / ml. The average number of spermatozoa in treatment one was 10.432 million / ml and the average number of spermatozoa in treatment two was 5.234 million / ml. This shows that exposure to cigarette smoke can reduce the number of spermatozoa from the control group to the first treatment by 42.56% and the second treatment shows a decrease from the first group by 34.65%.

scholarly journals Study of Kidney Repair Mechanism of Cassava Leaf Extract on Gentamicin induced Nephrotoxicity

2017 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Rena Normasari ◽  
Rosita Dewi ◽  
Sheilla Rachmania
Keyword(s):  
Statistical Analysis ◽  
Vitamin C ◽  
Leaf Extract ◽  
Renal Damage ◽  
Serum Levels ◽  
Repair Mechanism ◽  
Control Groups ◽  
Treatment Groups ◽  
Cassava Leaves

Drugs consumption like gentamicin caused damage and decreased of kidney function. Cassava leaf contain lots of micronutrients, such as vitamin C, vitamin A and carotenoids, which also as an antioxidant. The results of the study conducted by Almashuri proved that cassava leaf have high levels of carotenoid compounds. The purpose of this study was to determine the effect of cassava leaf extract on the repairment of renal damage in mice in vivo. This research is true experimental using mice (mus musculus) and were grouped into 5 groups. Two control groups (positive and negative) and 3 treatment groups (cassava leaves 150, 300, and 450 mg / day). Gentamicin is given every day for 14 days and then observed on serum levels of creatinine along with histopathological scores of kidneys’ damage. Statistical analysis showed a significant reduction in serum levels of creatinine (P <0.05) and a significant decrease in renal histopathologic score. This study suggests that cassava leaf extract can repair kidney damage as a result gentamicin-induced nephrotoxicity in mice.Keywords: cassava leaf extract, creatinin, kidney histopathology

scholarly journals A Traditional Chinese Medicine Plant Extract Prevents Alcohol-Induced Osteopenia

2021 ◽  
Vol 12 ◽  
Author(s):  
Dongyang Qian ◽  
Hui Zhou ◽  
Pan Fan ◽  
Tao Yu ◽  
Anish Patel ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Alcohol Consumption ◽  
Traditional Chinese Medicine ◽  
Bone Diseases ◽  
Syzygium Aromaticum ◽  
Herbal Extracts ◽  
Male Mice ◽  
Middle Aged ◽  
Excessive Alcohol Consumption ◽  
Excessive Alcohol

Traditional Chinese medicine (TCM) has been practiced in the treatment of bone diseases and alcoholism. Chronic excessive alcohol use results in alcohol-induced bone diseases, including osteopenia and osteoporosis, which increases fracture risk, deficient bone repair, and osteonecrosis. This preclinical study investigated the therapeutic effects of TCM herbal extracts in animal models of chronic excessive alcohol consumption-induced osteopenia. TCM herbal extracts (Jing extracts) were prepared from nine Chinese herbal medicines, a combinative herbal formula for antifatigue and immune regulation, including Astragalus, Cistanche deserticola, Dioscorea polystachya, Lycium barbarum, Epimedium, Cinnamomum cassia, Syzygium aromaticum, Angelica sinensis, and Curculigo orchioides. In this study, Balb/c male mice were orally administrated alcohol (3.2 g/kg/day) with/without TCM herbal extracts (0.125 g/kg, 0.25 g/kg, or 0.5 g/kg) by gavage. Our results showed that after 50 days of oral administration, TCM herbal extracts prevented alcohol-induced osteopenia demonstrated by μ-CT bone morphological analysis in young adults and middle-aged/old Balb/c male mice. Biochemical analysis demonstrated that chronic alcohol consumption inhibits bone formation and has a neutral impact on bone resorption, suggesting that TCM herbal extracts (Jing extracts) mitigate the alcohol-induced abnormal bone metabolism in middle-aged/old male mice. Protocatechuic acid, a natural phenolic acid in Jing extracts, mitigates in vivo alcohol-induced decline of alkaline phosphatase (ALP) gene expression in the bone marrow of Balb/c male mice and in vitro ALP activity in pre-osteoblast MC3T3-E1 cells. Our study suggests that TCM herbal extracts prevent chronic excessive alcohol consumption-induced osteopenia in male mice, implying that traditional medicinal plants have the therapeutic potential of preventing alcohol-induced bone diseases.

scholarly journals The Effect of Haramounting Leaf Ethanol Extract (Rhodomyrtus tomentosa (Aiton) Hassk.) on the Number of Leukocyte Type and Histology of Mice Pulmo (Mus Musculus L.) Exposed to Electronic Cigarette

2019 ◽  
Vol 7 (11) ◽  
pp. 1750-1756 ◽  
Author(s):  
Syafruddin Ilyas ◽  
Finn Murdella ◽  
Salomo Hutahaean ◽  
Putri Cahaya Situmorang
Keyword(s):  
Ethanol Extract ◽  
Electronic Cigarette ◽  
Control Group ◽  
Male Mice ◽  
Treatment Groups ◽  
Anatomy Laboratory ◽  
Leukocyte Counts ◽  
Ethanol Extracts ◽  
North Sumatra ◽  
Rhodomyrtus Tomentosa

BACKGROUND: Haramonting leaves have a large antioxidant activity as inhibitors oxidation and form non-free radical compounds that are not reactive and relatively stable. Electronic smoking is a choice who slowly want to quit conventional smoking.AIM: This research aimed to determine the effect of ethanol extract on hard mounting leaves on leukocyte counts and histology Pulmo on male mice exposed to an electronic cigarette.METHODS: The samples used in this study were healthy male mice aged 8-11 weeks and weight of 25-30 g as many as 25 individuals and ethanol extracts of hard mounting leaves with a dose of 100 mg/kgBW, 200 mg/kgBW and 300 mg/kgBW. Blood tests were carried out at the Health Laboratory of North Sumatra Office, and the preparation of Pulmo histology was carried out at the Pathology and Anatomy Laboratory of Adam Malik General Hospital MedanRESULT: There were significant differences between the control group and all of the treatment groups in the number of lymphocytes, neutrophils, basophils and eosinophils. There was a tendency that electronic cigarette smoke could cause damage to pulmo tissue.CONCLUSION: The results of this study indicate that the ethanol extract of hard mounting leaves could help the immune system in mice exposed to an electronic cigarette.

scholarly journals Bromocriptine in Parkinson’s Disease: Results Obtained With High and Low Dose Therapy

1984 ◽  
Vol 11 (S1) ◽  
pp. 225-228 ◽  
Author(s):  
J. David Grimes
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Statistical Analysis ◽  
Low Dose ◽  
Response Rate ◽  
De Novo ◽  
High Dose ◽  
High Dose Therapy ◽  
Treatment Groups ◽  
Dose Therapy

ABSTRACTThe results obtained with high and low dose bromocriptine therapy were compared in a review assessing the per cent of patients showing improvement (not taking account of the extent of improvement). It is concluded that the response rate with low dose bromocriptine is as good as that obtained with high dose therapy for both de novo and levodopa treated patients. The incidence of adverse effects is similar in the high and low dose treatment groups: More levodopa reduction results in a higher daily bromocriptine requirement. A statistical analysis of 61 bromocriptine-levodopa treated patients showed no positive correlation between bromocriptine dose and severity or duration of Parkinson’s disease.

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