Isolation, characterization and expression analysis of novel water deficit stress-responsive DEEPER ROOTING 1 (DRO1) gene from drought-tolerant Erianthus arundinaceus

Drought response in wheat is considered a highly complex process, since it is a multigenic trait; nevertheless, breeding programs are continuously searching for new wheat varieties with characteristics for drought tolerance. In a previous study, we demonstrated the effectiveness of a mutant known as RYNO3936 that could survive 14 days without water. In this study, we reveal another mutant known as BIG8-1 that can endure severe water deficit stress (21 days without water) with superior drought response characteristics. Phenotypically, the mutant plants had broader leaves, including a densely packed fibrous root architecture that was not visible in the WT parent plants. During mild (day 7) drought stress, the mutant could maintain its relative water content, chlorophyll content, maximum quantum yield of PSII (Fv/Fm) and stomatal conductance, with no phenotypic symptoms such as wilting or senescence despite a decrease in soil moisture content. It was only during moderate (day 14) and severe (day 21) water deficit stress that a decline in those variables was evident. Furthermore, the mutant plants also displayed a unique preservation of metabolic activity, which was confirmed by assessing the accumulation of free amino acids and increase of antioxidative enzymes (peroxidases and glutathione S-transferase). Proteome reshuffling was also observed, allowing slow degradation of essential proteins such as RuBisCO during water deficit stress. The LC-MS/MS data revealed a high abundance of proteins involved in energy and photosynthesis under well-watered conditions, particularly Serpin-Z2A and Z2B, SGT1 and Calnexin-like protein. However, after 21 days of water stress, the mutants expressed ABC transporter permeases and xylanase inhibitor protein, which are involved in the transport of amino acids and protecting cells, respectively. This study characterizes a new mutant BIG8-1 with drought-tolerant characteristics suited for breeding programs.

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Relationships between physiological traits and productivity of sugarcane in response to water deficit

The Journal of Agricultural Science ◽

10.1017/s0021859612000834 ◽

2012 ◽

Vol 152 (1) ◽

pp. 104-118 ◽

Cited By ~ 16

Author(s):

M. DE A. SILVA ◽

J. L. JIFON ◽

J. A. G. DA SILVA ◽

C. M. DOS SANTOS ◽

V. SHARMA

Keyword(s):

Water Deficit ◽

Field Studies ◽

Water Deficit Stress ◽

Chlorophyll Contents ◽

Physiological Variables ◽

Drought Tolerant ◽

Relative Water ◽

Leaf Relative Water Content ◽

Sugarcane Productivity ◽

Response To Water

SUMMARYThe relationships between physiological variables and sugarcane productivity under water deficit conditions were investigated in field studies during 2005 and 2006 in Weslaco, Texas, USA. A total of 78 genotypes and two commercial varieties were studied, one of which was drought-tolerant (TCP93-4245) and the other drought-sensitive (TCP87-3388). All genotypes were subjected to two irrigation regimes: a control well-watered treatment (wet) and a moderate water-deficit stress (dry) treatment for a period of 90 days. Maximum quantum efficiency of photosystem II (Fv/Fm), estimated chlorophyll content (SPAD index), leaf temperature (LT), leaf relative water content (RWC) and productivity were measured. The productivity of all genotypes was, on average, affected negatively; however, certain genotypes did not suffer significant reduction. Under water deficit, the productivity of the genotypes was positively and significantly correlated with Fv/Fm, SPAD index and RWC, while LT had a negative correlation. These findings suggest that genotypes exhibiting traits of high RWC values, high chlorophyll contents and high photosynthetic radiation use efficiency under low moisture availability should be targeted for selection and variety development in programmes aimed at improving sugarcane for drought prone environments.

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Effect of Water Deficit Stress on Seedling Biomass and Physio-Chemical Characteristics in Different Species of Wheat Possessing the D Genome

Agronomy ◽

10.3390/agronomy9090522 ◽

2019 ◽

Vol 9 (9) ◽

pp. 522 ◽

Cited By ~ 2

Author(s):

Alireza Pour-Aboughadareh ◽

Mansoor Omidi ◽

Mohammad Reza Naghavi ◽

Alireza Etminan ◽

Ali Ashraf Mehrabi ◽

...

Keyword(s):

Water Deficit ◽

Principal Component ◽

Water Deficit Stress ◽

Tolerance Index ◽

D Genome ◽

Drought Tolerant ◽

Seedling Biomass ◽

Primary Yield ◽

Psii Photochemistry ◽

Relative Water

Wild relatives of wheat serve as an extraordinary source of variability for breeding programs due to their capabilities to respond to various environmental stresses. Here, we investigated some species possessing a D genome (T. aestivum, Ae. tauschii, Ae. crassa and Ae. cylindrica) in terms of relative water content (RWC), stomatal conductance (Gs), relative chlorophyll content, initial fluorescence (Fo), maximum quantum yield of PSII (Fv/Fm), maximum primary yield of PSII photochemistry (Fv/Fo), as well as shoot fresh and dry biomasses under control and water deficit conditions. Our results revealed that water deficit negatively affected all traits; shoot fresh weight, Gs and RWC showed the highest reduction compared to the control condition. Principal component analysis (PCA) identified two PCs that accounted for 53.36% of the total variation in the water deficit conditions. Correlation analysis and PCA-based biplots showed that stress tolerance index (STI) is significantly associated with Fv/Fm and Fv/Fo under water stress conditions, suggesting that these are the best parameters to evaluate when screening for tolerant samples at the seedling stage. We identified 19 accessions from Ae. crassa and one from Ae. tauschii as the most tolerant samples. In conclusion, Ae. crassa might provide an ideal genetic resource for drought-tolerant wheat breeds.

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Response of Virginia (flue-cured) tobacco genotypes to water-deficit stress

Archives of Biological Sciences ◽

10.2298/abs160202085s ◽

2017 ◽

Vol 69 (1) ◽

pp. 119-127 ◽

Cited By ~ 2

Author(s):

Lydia Shtereva ◽

Elisaveta Stoimenova ◽

Marina Drumeva-Yoncheva ◽

Bistra Michailova ◽

Tanja Kartzeva ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Plant Growth ◽

Water Deficit ◽

Rapid Screening ◽

Water Deficit Stress ◽

Area Index ◽

Drought Tolerant ◽

Rapid Screening Test ◽

Mda Content ◽

Whole Plants

The effect of prolonged water deficit on four Virginia (flue-cured) tobacco genotypes, Line 842, Oxford 207, RG11 and Virgin D, was analyzed in whole plants. Drought stress was induced by withholding irrigation and subjecting plants to low, moderate and severe regimes. Some growth indices such as fresh weight, plant growth rate, number, color and area of new developed leaves, as well as proline, hydrogen peroxide (H2O2) and malondialdehyde (MDA) content as a measure of oxidative stress were investigated to examine the role of genotype in water-deficit tolerance. Under stress, the weight of the aboveground parts of plants, plant growth height, number of new developed leaves and leaf area index decreased with the severity of treatment. The stressed plants accumulated more proline, malonildialdehide and hydrogen peroxide than control non-stressed plants under water-deficit conditions. The results showed that among the genotypes, Virgin D (VD) was the most sensitive to drought, while L 842 and Oxford 207 were moderately tolerant; RG11 was drought-tolerant. This suggests that the correlation between the physiological traits and level of antioxidative response exists and therefore it could be used as a rapid screening test to evaluate the drought tolerance of tobacco.

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Screening of Iranian Tarragon Accessions Based on Physiological and Phytochemical Responses Under Water Deficit: Applications for Development and Breeding

10.21203/rs.3.rs-484006/v1 ◽

2021 ◽

Author(s):

Hasan Mumivand ◽

Amin Ebrahimi ◽

Alireza Shayganfar ◽

Hamid Hassaneian Khoshro

Keyword(s):

Secondary Metabolites ◽

Water Deficit ◽

Yield Potential ◽

High Yield ◽

Water Deficit Stress ◽

Drought Tolerant ◽

Severe Water Deficit ◽

Coumarin Compounds ◽

Different Response ◽

New Compounds

Abstract In this study, screening of tarragon accessions based on physiological and phytochemical traits was investigated under water deficit. The compounding impacts of water deficit * accessions significantly altered the chlorophyll and carotenoid contetnt, malondialdehyde, electrolyte leakage, superoxide dismutase, catalase and ascorbate peroxidase. The HPLC analyse revealed the presence of chlorogenic, syringic, ferulic, vanilic, chicoric and p-coumaric acids as major phenolic acids, while quercetin and herniarin were detected as the predominant flavonoid and coumarin compounds in the extracts. The quality and quantity of tarragon secondary metabolites were impacted by water deficit, suggesting that drought stress either increased the amounts of some common compounds or introduced some new compounds that were not present under normal conditions. In some genotypes, the content of some secondary metabolites in tarragon had the highest values under severe water deficit stress. The results indicated that Hamadan, Varamin and Estahbanat accessions could be introduced as tolerant accessions. Due to the very different response of tarragon accessions to water deficit and the existence of diversity between these accessions, the findings of the present study could be an effective step in identifying and achieving homogeneous, drought-tolerant and high-yield potential accessions, and may help tarragon breeding programs and development of cultivation.

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Antioxidant, physiological and biochemical responses of drought susceptible and drought tolerant mustard (Brassica juncea L) genotypes to rhizobacterial inoculation under water deficit stress

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2019.08.018 ◽

2019 ◽

Vol 143 ◽

pp. 19-28 ◽

Cited By ~ 4

Author(s):

S. Bandeppa ◽

Sangeeta Paul ◽

Jyoti Kumar Thakur ◽

N. Chandrashekar ◽

Deepika Kumar Umesh ◽

...

Keyword(s):

Water Deficit ◽

Brassica Juncea ◽

Water Deficit Stress ◽

Biochemical Responses ◽

Drought Tolerant ◽

Physiological And Biochemical

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Ascorbic acid alleviates water deficit induced growth inhibition in wheat seedlings by modulating levels of endogenous antioxidants

Biologia ◽

10.1515/biolog-2016-0050 ◽

2016 ◽

Vol 71 (4) ◽

Cited By ~ 7

Author(s):

Navdeep Singh ◽

Rachana D. Bhardwaj

Keyword(s):

Ascorbic Acid ◽

Water Deficit ◽

Seed Priming ◽

Antioxidant Response ◽

Shoot Length ◽

Water Deficit Stress ◽

Wheat Cultivars ◽

Wheat Seedlings ◽

Drought Tolerant ◽

Endogenous Antioxidants

AbstractThe present investigation envisaged the potential of ascorbic acid through seed priming on the seedling growth and antioxidant response in three wheat cultivars i.e., PBW 644 (Drought tolerant), PBW 621 and HD 2967 (Drought sensitive) grown under water deficit stress. Imposition of water deficit stress reduced the root and shoot lengths by more than 11% in PBW 644, 18% in PBW 621 and 9% in HD 2967. On exposure to water deficit stress, catalase (CAT) was upregulated in the roots of all the three cultivars but declined in the shoots. Ascorbate peroxidase (APX) activity increased in stressed plants of tolerant cultivar but declined in sensitive cultivars except the shoots of HD 2967. Under water deficit stress, seed priming with 400 μM of ascorbic acid increased shoot length of all the three cultivars by more than 19% when compared to stressed seedlings. Further, it enhanced the drought tolerance of PBW 644 by upregulating superoxide dismutase (SOD), CAT, APX and glutathione reductase (GR) enzymes accompanied by an increase in glycine betaine and H

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Performance of Drought-tolerant Varieties of Maize (Zea mays L.) under Water Deficit Stress Condition in the North Region of Cameroon

Journal of Experimental Agriculture International ◽

10.9734/jeai/2020/v42i330485 ◽

2020 ◽

pp. 64-73

Author(s):

Paul Sounou Alioum ◽

Jacques Djida Housseini ◽

Tontsa Noelle Hortense Mafouasson

Keyword(s):

Water Deficit ◽

Stress Condition ◽

Positive Control ◽

Negative Control ◽

Water Deficit Stress ◽

Ecological Zone ◽

Drought Tolerant ◽

Maize Genotypes ◽

Maize Varieties ◽

Maize Variety

Aims: The present study aimed to assess the performance of three drought-tolerant maize genotypes (DTSTRSYNY-2 (V1), WHITE DTSTRSYN (V2) and TZL COMP.4DTF2 (V3)) developed by IITA under water deficit stress conditions. Place and Duration of Study: Experiment was carried out at Sanguere-Paul in the Soudano-Sahelian agro-ecological zone, North region, Cameroon from September to December 2019. Methodology: Experimental design employed was the Randomized Complete Block Design (RCBD) of 5 treatments corresponding to different maize varieties in 2 replicates. The drought-sensitive CMS 9015 (V4) and the drought-tolerant EVDT99 QPM W (V5) maize varieties performed by IRAD was used as negative and positive controls, respectively. For each maize variety, growth and production parameters were recorded alongside the experiment and grain yield was calculated. Results: Results showed that the three drought-tolerant maize genotypes and the positive control maize variety assessed significantly exhibited a good performance under water stress condition regarding the growth and production traits registered compared to the negative control. Concerning growth parameters, the drought-tolerant maize varieties V1, V2 and V3 presented short anthesis-silking interval (1-1.5 day), good plant aspect, low number of dried leaves above ear (8-15 leaves) and less dead plants (1.25 -1.50 plant)compared to the controls. For V1, V2 and V3 drought-tolerant maize varieties, high yields of 3.24, 3.58 and 3.73 ton/ha, respectively, were obtained compared to the controls(1.13 ton/ha for the negative control V4 and 2.27 ton/ha for the positive control V5). Conclusion: Thus, the three drought-tolerant maize varieties showed good performance in the Soudano-Sahelian agro-ecological zone of Cameroon and should be adopted by farmers to increase and improve the maize production in the country, even under water deficit conditions.

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Expression Analysis of Sugarcane Aquaporin Genes under Water Deficit

Journal of Nucleic Acids ◽

10.1155/2013/763945 ◽

2013 ◽

Vol 2013 ◽

pp. 1-14 ◽

Cited By ~ 10

Author(s):

Manassés Daniel da Silva ◽

Roberta Lane de Oliveira Silva ◽

José Ribamar Costa Ferreira Neto ◽

Ana Carolina Ribeiro Guimarães ◽

Daniela Truffi Veiga ◽

...

Keyword(s):

Molecular Markers ◽

Plant Breeding ◽

Water Deficit ◽

Expression Analysis ◽

Higher Plants ◽

Expression Patterns ◽

Future Studies ◽

Drought Tolerant ◽

Stress Acclimation ◽

Validation Strategy

The present work is a pioneer study specifically addressing the aquaporin transcripts in sugarcane transcriptomes. Representatives of the four aquaporin subfamilies (PIP, TIP, SIP, and NIP), already described for higher plants, were identified. Forty-two distinct aquaporin isoforms were expressed in four HT-SuperSAGE libraries from sugarcane roots of drought-tolerant and -sensitive genotypes, respectively. At least 10 different potential aquaporin isoform targets and their respective unitags were considered to be promising for future studies and especially for the development of molecular markers for plant breeding. From those 10 isoforms, four (SoPIP2-4,SoPIP2-6,OsPIP2-4, andSsPIP1-1) showed distinct responses towards drought, with divergent expressions between the bulks from tolerant and sensitive genotypes, when they were compared under normal and stress conditions. Two targets (SsPIP1-1 andSoPIP1-3/PIP1-4) were selected for validation via RT-qPCR and their expression patterns as detected by HT-SuperSAGE were confirmed. The employed validation strategy revealed that different genotypes share the same tolerant or sensitive phenotype, respectively, but may use different routes for stress acclimation, indicating the aquaporin transcription in sugarcane to be potentially genotype-specific.

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