Assessment of interleukin-17 and interleukin-4 in blood serum in posttraumatic osteomyelitis of the mandible

2019 ◽  
pp. 62-64
Author(s):  
E.V. Paskova ◽  
K.I. Shahgeldyan ◽  
E.V. Markelova
Keyword(s):  
Blood Serum ◽  
Interleukin 4 ◽  
Interleukin 17 ◽  
Posttraumatic Osteomyelitis

scholarly journals ROLE OF CYTOKINE-MEDIATED MECHANISMS IN DEVELOPMENT OF POST-TRAUMATIC MANDIBULAR OSTEOMYELITIS

2019 ◽  
Vol 21 (5) ◽  
pp. 953-958
Author(s):  
E. V. Paskova ◽  
E. V. Markelova ◽  
K. I. Shakhgeldyan ◽  
B. I. Geltser ◽  
A. B. Kriger
Keyword(s):  
Prognostic Significance ◽  
Mandibular Fracture ◽  
Interleukin 4 ◽  
Mandibular Fractures ◽  
Interleukin 17 ◽  
Posttraumatic Osteomyelitis ◽  
Post Traumatic ◽  
Binary Classifiers ◽  
Mixed Saliva

Osteomyelitis of the lower jaw is one of the urgent problems of modern medicine. There are many reasons for the evolvement of purulent necrotic processes of the jaw bones, including the role of disorders in the systems of innate and adaptive immunity. The aim of the study was to determine the content of TNFα, IL-17, IL-4 in serum and mixed saliva in patients with uncomplicated mandibular fractures and posttraumatic osteomyelitis to determine the possibility of using these indicators for early diagnosis of posttraumatic complications. The article presents the results of a study of tumor necrosis factor α (TNFα), interleukin-17 (IL-17) and interleukin-4 (IL-4) cytokines in serum and mixed saliva in patients with uncomplicated mandibular fracture and post-traumatic osteomyelitis at the first and tenth days of observation. By means of single-layer neural networks, binary classifiers were built, allowing patients to be stratified by the clinical form of the disease and to predict its course. The probability of uncomplicated mandibular fracture is described by the ratio P = 1/(1+e-z), where the index z is determined by the level of TNFα, IL-17, and IL-4 at the first and tenth day of observation. The simulation confirmed high prognostic significance of serum TNFα and IL-17 for early verification of posttraumatic osteomyelitis, which was confirmed by the OTC and ROC indices, which varied from 87 to 100% in different models. Models 4 and 5, where TNFα recorded on the tenth day of the study was used as predictors, and a combination of TNFα and IL-17 obtained on the first day of hospitalization, were the most accurate. Modeling the results of the study of immunological indicators in the mixed saliva showed that the predictive properties have only IL-4 and IL-17, was on the tenth day of hospitalization that distinguishes these binary classifiers from similar indexes, derive from the levels of cytokines in blood serum. The results of the study indicate the important role of disorders in the system of рro- and anti-inflammatory cytokines in pathogenesis of post-traumatic osteomyelitis.

Levels interleukine-4 and interleukin-17 (IL-4, IL-17) of blood in patients with habitual recurrent pregnancy loss, which occurred in a loop in vitro fertilization

2016 ◽  
pp. 137-139
Author(s):  
K.P. Golovatyuk ◽  
Keyword(s):  
In Vitro Fertilization ◽  
Conditioned Medium ◽  
Mononuclear Cells ◽  
Recurrent Miscarriage ◽  
Interleukin 4 ◽  
Control Group ◽  
Interleukin 17 ◽  
Vitro Fertilization ◽  
Blood Mononuclear Cells

The objective: was to investigate the levels of cytokines IL-4 and IL-17 in serum and conditioned medium cultures of blood mononuclear cells (MNC) and evaluation association between their products and miscarriage, which occurred in IVF cycles. Patients and methods. We observed 240 patients with recurrent miscarriage, came in IVF cycles, and 100 apparently healthy fertile women in the control group. The concentrations of IL-4 and IL-17 in serum and conditioned medium of MNC cultures were determined. Results. The levels of IL-4 in the serum and conditioned medium in spontaneous and stimulated mitogen secretion was not significantly different from those in the control group, whereas IL-17 levels were higher than those in the control group serum, in conditioned media of stimulated and non-stimulated MNCs. Conclusion. Disregulation of activity of circulating blood mononuclear cells in women with recurrent miscarriage that followed IVF, is accompanied by increased secretion of IL-17 and almost constant production of IL-4 on the back of high stimulation index of production of these cytokines. Key words: in vitro fertilization, miscarriage, interleukin-4, interleukin-17, serum stimulated and non-stimulated mononuclear blood.

scholarly journals Reduction of interleukin‐17–induced inhibition of chondrocyte proteoglycan synthesis in intact murine articular cartilage by interleukin‐4

2000 ◽  
Vol 43 (6) ◽  
pp. 1300-1306 ◽  
Author(s):  
Erik Lubberts ◽  
Leo A. B. Joosten ◽  
Fons A. J. Van De Loo ◽  
Liduine A. M. Van Den Bersselaar ◽  
Wim B. Van Den Berg
Keyword(s):  
Articular Cartilage ◽  
Interleukin 4 ◽  
Proteoglycan Synthesis ◽  
Interleukin 17

scholarly journals Continued Elevation of Plasma IL-4 and IL-17 Predicts the Progression from VMC to DCM

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Z. H. Wang ◽  
Y. H. Liao ◽  
J. Yuan ◽  
X. J. Jin ◽  
M. Yu ◽  
...  
Keyword(s):  
Viral Myocarditis ◽  
Interleukin 4 ◽  
Interleukin 17 ◽  
Newly Diagnosed ◽  
Multicenter Observational Study ◽  
Viral Antibodies ◽  
Blood Tests ◽  
High Incidence ◽  
Plasma Cytokines ◽  
Prospective Multicenter Observational Study

Objective. To investigate plasma cytokines (interferon gamma, interleukin-4, and interleukin-17) in patients with viral myocarditis (VMC) and evaluate their predictive value in the progression from VMC to dilated cardiomyopathy (DCM). Methods. A prospective, multicenter, observational study included 536 patients with newly diagnosed VMC admitted in cardiology departments of 24 tertiary super specialised university-affiliated hospitals in the China registry from January 2012 to June 2016. Demographics and clinical characteristics at baseline and after three months were collected, including laboratory blood tests, ECG, echocardiography, and drug treatment in each participating site. The plasma anti-viral antibodies (Abs), anti-heart autoimmune Abs, and cytokines were detected by ELISA. Results. Of the 536 patients, 534 were included for analysis after two patients died in less than a month. The plasma levels of IFN-γ, IL-4, and IL-17 were continually higher in patients with incident DCM than in those without incident DCM at baseline, from the 1st month and the 3rd month; all had a P value of <0.0001. There was a positive correlation between IL-4 and LVEDd (r=0.30, P<0.0001) and between IL-17 and LVEDd (r=0.11, P=0.02). When all these covariates have entered the model simultaneously, elevated IL-4 and IL-17 were still significantly associated with DCM incidence. The RR (95% CI) of DCM incidence were 1.04 (1.02-1.06) for IL-4 and 5.24 (2.81-9.79) for IL-17. Conclusion. The continued elevation of plasma IL-4 and IL-17 in VMC patients were associated with a high incidence of DCM at three months, and these two cytokines were independent predictors for the progression from VMC to DCM.

scholarly journals Mycobacterium tuberculosis Culture Filtrate Proteins plus CpG Oligodeoxynucleotides Confer Protection to Mycobacterium bovis BCG-Primed Mice by Inhibiting Interleukin-4 Secretion

2009 ◽  
Vol 77 (12) ◽  
pp. 5311-5321 ◽  
Author(s):  
Denise Morais da Fonseca ◽  
Celio Lopes Silva ◽  
Pryscilla Fanini Wowk ◽  
Marina Oliveira e Paula ◽  
Simone Gusmão Ramos ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Bovis ◽  
Culture Filtrate ◽  
Vaccine Development ◽  
Interleukin 4 ◽  
Interleukin 17 ◽  
Bcg Vaccination ◽  
Cpg Oligodeoxynucleotides ◽  
Culture Filtrate Proteins ◽  
Ifn Γ

ABSTRACT Culture filtrate proteins (CFP) are potential targets for tuberculosis vaccine development. We previously showed that despite the high level of gamma interferon (IFN-γ) production elicited by homologous immunization with CFP plus CpG oligodeoxynucleotides (CFP/CpG), we did not observe protection when these mice were challenged with Mycobacterium tuberculosis. In order to use the IFN-γ-inducing ability of CFP antigens, in this study we evaluated a prime-boost heterologous immunization based on CFP/CpG to boost Mycobacterium bovis BCG vaccination in order to find an immunization schedule that could induce protection. Heterologous BCG-CFP/CpG immunization provided significant protection against experimental tuberculosis, and this protection was sustained during the late phase of infection and was even better than that conferred by a single BCG immunization. The protection was associated with high levels of antigen-specific IFN-γ and interleukin-17 (IL-17) and low IL-4 production. The deleterious role of IL-4 was confirmed when IL-4 knockout mice vaccinated with CFP/CpG showed consistent protection similar to that elicited by BCG-CFP/CpG heterologous immunization. These findings show that a single dose of CFP/CpG can represent a new strategy to boost the protection conferred by BCG vaccination. Moreover, different immunological parameters, such as IFN-γ and IL-17 and tightly regulated IL-4 secretion, seem to contribute to the efficacy of this tuberculosis vaccine.

scholarly journals Interleukin-17-producing decidual CD4+ T cells are not deleterious for human pregnancy when they also produce interleukin-4

2016 ◽  
Vol 14 (1) ◽  
Author(s):  
Letizia Lombardelli ◽  
Federica Logiodice ◽  
Maryse Aguerre-Girr ◽  
Ornela Kullolli ◽  
Herman Haller ◽  
...  
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
Interleukin 4 ◽  
Interleukin 17 ◽  
Human Pregnancy

scholarly journals Dynamics of cytokine activity of cerebrospinal fluid and blood serum of carnivores with pain syndrome

2021 ◽  
pp. 52-55
Author(s):  
Sergey Dmitrievich Klyukin ◽  
Vladimir Vasilyevich Salautin ◽  
Sergey Vasilyevich Kozlov ◽  
Nikolai Alexandrovich Pudovkin ◽  
Daniil Sergeevich Frolov
Keyword(s):  
Cerebrospinal Fluid ◽  
Blood Serum ◽  
Pain Syndrome ◽  
Interleukin 4 ◽  
Necrosis Factor Alpha ◽  
Ifn Gamma ◽  
Factor Alpha ◽  
Experimental Group ◽  
Necrosis Factor

New data on the mechanisms of pain syndrome development in dogs and cats were obtained, in particular, the role of anti - inflammatory cytokines: interleukin-4 (IL-4), interleukin-6 (IL - 6), interferon - gamma (IFN - gamma), tumor necrosis factor - alpha (TNF-alfa) in the formation and manifestation of this pathology was determined. The results obtained allow us to assess the dynamics of the immune status of animals with pain syndrome. Cytokines: IL-4, IL-6, and IFN - gamma showed high significance in the diagnosis of pain syndrome. Thus, the values of IL-4 in the cerebrospinal fluid and blood serum in dogs in the first experimental group were 3.5 times and 3.4 times higher in the second experimental group than in the control, in cats of the first experimental group-4.3 and 4.6 times in the second experimental group. As well as IL-4, both IL - 6 and IFN - gamma showed their high activity, in contrast to TNF-alfa, which was less sensitive to changes in pain syndrome.

scholarly journals Increased serum levels of interleukin-6 in erythema nodosum leprosum suggest its use as a biomarker

2021 ◽  
Vol 87 ◽  
pp. 190-198
Author(s):  
Fátima Regina Vilani-Moreno ◽  
Vânia Nieto Brito-de-Souza ◽  
Sônia Maria Usó Ruiz Silva ◽  
Adriana Sierra Assêncio Almeida Barbosa ◽  
Beatriz Gomes Carreira Sartori ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Interleukin 2 ◽  
Serum Levels ◽  
Interleukin 10 ◽  
Interferon Γ ◽  
Interleukin 4 ◽  
Interleukin 17 ◽  
Erythema Nodosum Leprosum ◽  
Multibacillary Leprosy ◽  
Necrosis Factor

Background: Erythema nodosum leprosum (ENL) is a frequent complication of multibacillary leprosy that can result in significant morbidity, including peripheral nerve damage and physical disability. The identification of possible serum markers could be a valuable tool for the early detection of ENL. Aims: The purpose of this study was to evaluate selected serum mediators involved in the innate and adaptive immune responses to identify possible immunomarkers for ENL. Methods: The levels of interleukin-2, interleukin-4, interleukin-6, interleukin-10, interleukin-17, interferon-γ, tumor necrosis factor, nitric oxide and anti-phenolic glycolipid-I antibodies were measured in the sera of leprosy patients with ENL [at the beginning of reaction (M0) and 1 month later (M1)], and then compared with the levels of the same markers in patients with untreated multibacillary leprosy without ENL (controls with leprosy: CTRL) and healthy individuals (healthy controls: CTRH). Results: Significantly higher levels of serum interleukin-6 were observed in M0 than in CTRL. In addition, pairwise comparisons showed higher levels of interleukin-6 in M0 compared to M1. Levels of tumor necrosis factor were higher in M0 than in CTRL, with no significant difference between M0 and M1. There were no differences in the levels of interleukin-2, interleukin-4, interleukin-10, interleukin-17 or interferon-γ between groups. The CTRL group had higher levels of nitric oxide compared to M0 and M1. High levels of anti-phenolic glycolipid-I were observed in M0, M1 and CTRL than in CTRH. Limitations: Three patients were not assessed at M1, decreasing the number of evaluated patients from 14 to 11. Conclusion: High-serum levels of interleukin-6 were observed during ENL, primarily in patients with more severe reactions; levels decreased after specific therapy, suggesting a role for this cytokine in pathogenesis and its utility as an ENL biomarker. Further studies should explore whether interleukin-6 could also be used as a predictive marker for ENL or as a specific target for its treatment.

scholarly journals Cytokine Profile in Striated Muscle Laminopathies: New Promising Biomarkers for Disease Prediction

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1532
Author(s):  
Cristina Cappelletti ◽  
Irene Tramacere ◽  
Paola Cavalcante ◽  
Elisa Schena ◽  
Luisa Politano ◽  
...  
Keyword(s):  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Striated Muscle ◽  
Wide Spectrum ◽  
Serum Levels ◽  
Interleukin 4 ◽  
Interleukin 17 ◽  
Lmna Gene ◽  
New Therapeutic Approaches ◽  
Inflammatory Molecules

Laminopathies are a wide and heterogeneous group of rare human diseases caused by mutations of the LMNA gene or related nuclear envelope genes. The variety of clinical phenotypes and the wide spectrum of histopathological changes among patients carrying an identical mutation in the LMNA gene make the prognostic process rather difficult, and classical genetic screens appear to have limited predictive value for disease development. The aim of this study was to evaluate whether a comprehensive profile of circulating cytokines may be a useful tool to differentiate and stratify disease subgroups, support clinical follow-ups and contribute to new therapeutic approaches. Serum levels of 51 pro- and anti-inflammatory molecules, including cytokines, chemokines and growth factors, were quantified by a Luminex multiple immune-assay in 53 patients with muscular laminopathy (Musc-LMNA), 10 with non-muscular laminopathy, 22 with other muscular disorders and in 35 healthy controls. Interleukin-17 (IL-17), granulocyte colony-stimulating factor (G-CSF) and transforming growth factor beta (TGF-β2) levels significantly discriminated Musc-LMNA from controls; interleukin-1β (IL-1β), interleukin-4 (IL-4) and interleukin-8 (IL-8) were differentially expressed in Musc-LMNA patients compared to those with non-muscular laminopathies, whereas IL-17 was significantly higher in Musc-LMNA patients with muscular and cardiac involvement. These findings support the hypothesis of a key role of the immune system in Musc-LMNA and emphasize the potential use of cytokines as biomarkers for these disorders.

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