scholarly journals Ratios of CD64 expressed on neutrophils, monocytes, and lymphocytes may be a novel method for diagnosis of neonatal sepsis

2015 ◽  
Vol 9 (02) ◽  
pp. 175-181 ◽  
Author(s):  
Dai-Hua Fang ◽  
Cong-Hai Fan ◽  
Juan Li ◽  
Qi An ◽  
Hong Yao ◽  
...  
Keyword(s):  
Neonatal Sepsis ◽  
Evaluation Method ◽  
High Sensitivity ◽  
Receiver Operating Curve ◽  
The Novel ◽  
Cd64 Expression ◽  
Neutrophil Cd64 ◽  
Significant Difference ◽  
Novel Method ◽  
And Control

Introduction: Neutrophil CD64 expression has been demonstrated as an improved diagnostic marker of infection and sepsis. The purpose of this study was to develop a new method to evaluate neutrophil CD64 expression for diagnosis of neonatal sepsis. Methodology: Eighty neonates with neonatal sepsis (21 culture positive, 59 negative) were enrolled in this prospective study along with 19 neonates with no symptoms or signs of infection as controls. Expressions of CD64 on monocytes, lymphocytes, and neutrophils were evaluated with flow cytometry (FCM). Ratios were calculated with these levels of CD64 expression. Blood culture and other laboratory exams were done at the same time for the diagnosis of neonatal sepsis. Results were compared between the neonatal sepsis and control groups. Results: CD64 ratios showed significant difference between the groups (p < 0.01). Receiver operating curve (ROC) analysis showed that the CD64 ratios possessed high sensitivity (90%) and specificity (89.5%) in neonatal sepsis identification. Conclusions: The novel CD64 evaluation method, CD64 ratio, can be used as a supplementary method for diagnosis of neonatal sepsis.

scholarly journals A novel PS4 criterion approach based on symptoms of rare diseases and in-house frequency data in a Bayesian framework

2020 ◽  
Author(s):  
You Kyung Cho ◽  
Dhong-gun Won ◽  
Changwon Keum ◽  
Beom Hee Lee ◽  
Go Hun Seo ◽  
...  
Keyword(s):  
Rare Diseases ◽  
Odds Ratio ◽  
Case Control Study ◽  
Rare Variants ◽  
Bayesian Framework ◽  
The Novel ◽  
Significant Difference ◽  
Novel Method ◽  
Control Study ◽  
Criterion Approach

AbstractThe American College of Medical Genetics (ACMG) and Genomics/Association for Molecular Pathology (AMP) previously reported standardized guidance for the assessment of genetic variants. One of the criteria regarding the prevalence in a case-control study, PS4, is important due to its evidence of pathogenicity. Despite recent studies approaching gene- and disease-specific probands, interpretation of a variant to PS4 still has certain limitations for rare variants. Here, we suggest a generalized method, Bayesian odds ratio (BayesianOR), applicable to PS4 via decomposing a disease to its symptoms and applying a Bayesian framework. Using this approach, we demonstrate reproducibility of the calculation of the original odds ratio from well-studied epilepsy data and verify the applicability to in-house frequencies for various rare diseases. In addition, BayesianOR showed a significant difference in tendency with different ClinVar pathogenicity, using in-house data. Thus, the novel method described here should provide an improved interpretation of sequence variants. Furthermore, we anticipate that it will enhance the diagnosis of patients with rare diseases.

scholarly journals Novel method to decrease the exposure time of the extraction string of the ureteral stent and its efficiency and safety verification in the clinic

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
WenGang Hu ◽  
YaJun Song ◽  
Yang Li ◽  
YueHua Li ◽  
Jiao Mu ◽  
...  
Keyword(s):  
Exposure Time ◽  
Medical Costs ◽  
Demographic Characteristics ◽  
Ureteral Stent ◽  
Continuous Exposure ◽  
The Novel ◽  
Ureteral Stents ◽  
Stent Removal ◽  
Significant Difference ◽  
Novel Method

AbstractUreteral stent removal by an extraction string is advantageous. However, the increased risk of complications attributed to the continuous exposure of the string outside the urethra must be managed. This paper introduces a method to decrease the exposure time, and conducts a retrospective study to verify its efficiency and safety. A total of 231 male patients undergoing routine ureteroscopy (URS) were included, and all of them accepted indwelling ureteral stents with strings. Among them, 123 patients (Normal-S group) underwent the normal method to determine the length of string (Lstring), which was shortened to 4 cm (cm) past the urethral meatus; 108 patients (Novel-S group) underwent the novel method (Lstring = Lurethra + 2 cm), the length of urethra (Lurethra) was measured during ureteroscopy by ureteroscope body. The demographic characteristics, stent indwelling and removal-related variables, complications, and medical costs in each group were recorded. There was no significant difference in demographic characteristics, the rate of UTI, the operative duration of URS, or the VAS pain scores for stent removal between the 2 groups. For the Novel-S group, the stent dwelling time was longer, the self-rated discomfort and symptom, the stent dislodgement rate, the numbers of clinic or emergency visits and the overall medical cost post operation was lower in comparison with the Normal-S group, while the rate of removal of stents by hand was lower, the time for removing ureteral stents was longer. This novel method improved stenting comfort, avoided ureteral stent dislodgement, decreased complications, and lowered medical costs, it was safe and reliable and merits widespread application.

The evaluation of serum N-terminal prohormone brain-type natriuretic peptide, troponin-I, and high-sensitivity C-reactive protein levels in children with congenital heart disease

2014 ◽  
Vol 33 (11) ◽  
pp. 1158-1166 ◽  
Author(s):  
A Uner ◽  
M Doğan ◽  
M Ay ◽  
Ç Acar
Keyword(s):  
Septal Defect ◽  
Troponin I ◽  
High Sensitivity ◽  
Control Group ◽  
Asymptomatic Group ◽  
Reactive Protein ◽  
Significant Difference ◽  
Healthy Control ◽  
Hs Crp ◽  
And Control

Although advanced diagnostic and treatment methods are available, congenital heart disease (CHD) holds an important place among the causes of death within the first year of age. Therefore, several prognostic factors are needed for diagnosis and monitoring of these patients. In this study, which includes 66 CHD patients and 38 healthy control children, serum cardiac troponin-I (cTnI), high-sensitivity C-reactive protein (Hs-CRP), and N-terminal prohormone brain-type natriuretic peptide (NT-proBNP) levels were analyzed for their prognostics values. The patient groups were categorized and then evaluated as cyanotic ( n = 16), acyanotic ( n = 50), symptomatic ( n = 23), asymptomatic ( n = 43), and isolated ventricular septal defect (VSD)-isolated atrial septal defect (ASD) groups. Cyanotic group was statistically compared with acyanotic group, symptomatic group with asymptomatic group, and VSD group with ASD group. Between the cyanotic, acyanotic, and control groups; between symptomatic and asymptomatic groups; and between the VSD and ASD groups, significant difference was not showed for age ( p > 0.05). NT-proBNP was found to be significantly higher in the cyanotic group than acyanotic and control group, in the symptomatic group than asymptomatic group; and in the patient group than healthy control group ( p < 0.05). Between the groups of VSD and ASD, significant difference was not showed ( p > 0.05). The same comparison results for TnI and Hs-CRP were not significant ( p > 0.05). TnI and Hs-CRP were only found significantly higher in the patient group than healthy control group ( p < 0.05). Eventually, we think that NT-proBNP, Hs-CRP, and TnI might be used for clinical management and estimation of outcome of these disorders in the future and these also might be able to modify existing strategies, but much more studies are needed.

scholarly journals THE EFFECT OF VITAMIN D LEVELS ON INFLAMMATION IN PATIENTS WITH CHRONIC RENAL FAILURE

2020 ◽  
Vol 15 (4) ◽  
pp. 166-178
Author(s):  
Nilüfer Bulut ◽  
Mehmet Çağatay Taşkapan ◽  
Hülya Taşkapan
Keyword(s):  
Vitamin D ◽  
Renal Failure ◽  
High Sensitivity ◽  
Control Group ◽  
Reactive Protein ◽  
Vitamin D Levels ◽  
Significant Difference ◽  
Hs Crp ◽  
And Control ◽  
The Relationship

It is suggested that a number of environmental and genetic factors trigger the formation of progressive kidney damage and complications. One of these factors is inflammation, it occurs as a result of a series of mechanisms included within a number of cytokines. Vitamin-D, IL-6, PCT and hs-CRP are also valuable biomarkers in terms of mortality in dialysis patients in this sense. Vitamin D deficiency is common in patients with chronic kidney disease (CKD) and is associated with inflammation. In recent years, some randomized controlled trials have revealed the effect of Vitamin D on inflammation in CKD patients, but the results are conflicting. The aim of this study is to investigate the relationship between Vitamin D, high-sensitivity C Reactive Protein (hs-CRP), procalcitonin and IL-6, and to evaluate the relation of Vitamin D levels with inflammation in PD patients, HD patients and controls. This study was carried on with 40 patients receiving on hemodialysis treatment, 40 patients receiving peritoneal treatment with renal failure disease and with a control group consisting of 40 healthy individuals. Vitamin D levels were measured by HPLC, PCT and IL-6 levels were measured by chemiluminescent method, hs-CRP is measured by nephelometric method. For Vitamin D, there was no differences between the groups. For PCT, there was a significant difference between all groups. For IL-6, while there was no difference between peritoneal and hemodialysis groups, a significant difference was determined between the peritoneal dialysis and control groups. For hs-CRP, there was a significant difference among all groups. While correlation was found between serum PCT levels with IL-6 and hs-CRP, no correlations were found between serum PCT with Vitamin D levels. Although a correlation was found between serum IL-6 levels and hs-CRP, no correlation was detected between serum IL-6 and Vitamin D levels. No correlations were detected between Vitamin D andPCT, IL-6 and hs-CRP.

scholarly journals Biocompatibility and Comfort during Extended Wear of Mel4 Peptide-Coated Antimicrobial Contact Lenses

Antibiotics ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 58
Author(s):  
Parthasarathi Kalaiselvan ◽  
Debarun Dutta ◽  
Nagaraju Konda ◽  
Pravin Krishna Vaddavalli ◽  
Savitri Sharma ◽  
...  
Keyword(s):  
Ocular Surface ◽  
Contact Lenses ◽  
Front Surface ◽  
Back Surface ◽  
The Novel ◽  
Significant Difference ◽  
Delayed Reactions ◽  
And Control ◽  
Corneal Staining ◽  
Lens Wear

(1) Purpose: This study aimed to investigate the effects of Mel4 antimicrobial contact lenses (MACL) on the ocular surface and comfort during extended wear. (2) Methods: A prospective, randomised, double-masked, contralateral clinical trial was conducted with 176 subjects to evaluate the biocompatibility of contralateral wear of MACL. The wearing modality was 14-day extended lens wear for three months. The participants were assessed at lens dispensing, after one night, two weeks, one month and three months of extended wear and one month after study completion. (3) Results: There were no significant differences (p > 0.05) in ocular redness or palpebral roughness between Mel4 and control eyes at any of the study visits. There was no significant difference (p > 0.05) in corneal staining between Mel4 and control eyes. There were no significant differences in front surface wettability or deposits or back surface debris (p > 0.05). No statistically significant differences (p > 0.05) were found in comfort, dryness, CLDEQ-8 scores lens or edge awareness. There was no evidence for delayed reactions on the ocular surface after cessation of lens wear. (4) Conclusion: The novel MACLs showed similar comfort to control lenses and were biocompatible during extended wear. Thus, these lenses were compatible with the ocular surface.

A novel method for the determination of linting propensity of paper

TAPPI Journal ◽  
2012 ◽  
Vol 11 (10) ◽  
pp. 9-17
Author(s):  
ALESSANDRA GERLI ◽  
LEENDERT C. EIGENBROOD
Keyword(s):  
Ash Content ◽  
The Novel ◽  
Offset Printing ◽  
Excellent Correlation ◽  
End Conditions ◽  
Bottom Side ◽  
Novel Method ◽  
Total Fraction

A novel method was developed for the determination of linting propensity of paper based on printing with an IGT printability tester and image analysis of the printed strips. On average, the total fraction of the surface removed as lint during printing is 0.01%-0.1%. This value is lower than those reported in most laboratory printing tests, and more representative of commercial offset printing applications. Newsprint paper produced on a roll/blade former machine was evaluated for linting propensity using the novel method and also printed on a commercial coldset offset press. Laboratory and commercial printing results matched well, showing that linting was higher for the bottom side of paper than for the top side, and that linting could be reduced on both sides by application of a dry-strength additive. In a second case study, varying wet-end conditions were used on a hybrid former machine to produce four paper reels, with the goal of matching the low linting propensity of the paper produced on a machine with gap former configuration. We found that the retention program, by improving fiber fines retention, substantially reduced the linting propensity of the paper produced on the hybrid former machine. The papers were also printed on a commercial coldset offset press. An excellent correlation was found between the total lint area removed from the bottom side of the paper samples during laboratory printing and lint collected on halftone areas of the first upper printing unit after 45000 copies. Finally, the method was applied to determine the linting propensity of highly filled supercalendered paper produced on a hybrid former machine. In this case, the linting propensity of the bottom side of paper correlated with its ash content.

The Promising Rule of Neutrophil CD64 As an Early Diagnostic and Prognostic Marker in Neonatal Sepsis

PEDIATRICS ◽  
2016 ◽  
Vol 137 (Supplement 3) ◽  
pp. 457A-457A
Author(s):  
Heba E. Ossman ◽  
Shreen A. El-Masry ◽  
Nour M. Abdel Aal ◽  
Amira M. Mokhtar
Keyword(s):  
Prognostic Marker ◽  
Neonatal Sepsis ◽  
Neutrophil Cd64 ◽  
Early Diagnostic
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