scholarly journals Preoperative Microbiological Screening in Pediatric Assessment of Skeletal Dysplasias Cases Before Planned Orthopedic Surgery

2021 ◽  
Vol 9 (B) ◽  
pp. 281-285
Author(s):  
Milka Dikova ◽  
Svetla Todorova Nikolova ◽  
Alexandre Loukanov
Keyword(s):  
Orthopedic Surgery ◽  
Pathogenic Bacteria ◽  
Antibacterial Prophylaxis ◽  
Preoperative Treatment ◽  
Upper Respiratory Tract ◽  
Gram Positive ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Skeletal Dysplasias ◽  
Gram Positive Bacteria ◽  
Normal Microflora

We report the preoperative microbiological screening of gram positive bacteria as a promising diagnostic approach for precise pediatric assessment of children with skeletal dysplasias who have undergone planned orthopedic surgery within a period of 10 years. The study was conducted on children with 29 different clinical diagnoses with preoperatively measured haemoglobin, platelets, blood glucose, serum glutamic-oxaloacetic transaminase, serum glutamic-pyruvic transaminase, serum creatinine, total protein, and fibrinogen. The performed preoperative microbiological screening involved the examination of nasal and throat secretions aiming to detect carrier and/or colonization of potentially pathogenic microflora in these upper respiratory tract niches. The documented statistical data demonstrated that 50.8 % of the cases had normal microflora, however the rest 49.2 % of the patients had potentially pathogenic bacteria. Among them, S. aureus was identified in 39.3% (as methicillin-susceptible or MSSA) and in 3.3% of the children (as methicillin-resistant or MRSA). The other predominant gram-positive bacteria were S. pneumoniae, S. pyogenes, M. catarrhalis, and C. albicans in 1.6, 0.8, 2.4 and 2.4 %, respectively. The infected patients have been at significantly higher risk for staph infections after surgical procedures than those with normal microflora. The presented microbiological screening proves the need for obligatory preoperative eradication of MRSA from nose niche, oral preoperative treatment of Streptococcus pyogenes and personalized preoperative antibacterial prophylaxis. 

scholarly journals Antimicrobial Activity of Gardenia jasminoides Callus and Crude Leaf Extracts for some Organic Solvents against some Pathogenic Bacteria and Yeasts

2014 ◽  
Vol 8 (3) ◽  
pp. 40-45
Author(s):  
Zina Hashem Shehab ◽  
Huda Suhail Abid ◽  
Sumaya Fadhil Hamad ◽  
Sara Haitham
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Pathogenic Bacteria ◽  
Saccharomyces Boulardii ◽  
Gram Negative Bacteria ◽  
Proteus Vulgaris ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Gardenia Jasminoides

The study was conducted to evaluate the inhibitory activity of methanol extract of Gardenia jasminoides leaves compared with leaf crude extracts for some organic solvents namely Methanol, Ethanol, Petroleum ether, Asetone and Chloroform on growth of some pathogenic bacteria and yeast, which included four gram positive isolates Staphylococcus aureus, Enterococcus faecalis, Streptococcus pyogenes and Bacillus cereus and gram negative isolates Escherichia coli, Salmonella typhi, Proteus vulgaris and Pseudomonas aeruginosa and some yeasts Candida albicans and Saccharomyces boulardii, by using well diffusion method. The inhibitory activity of extracts in the tested bacterial strains and yeasts was varied according to the type of extracting solvents and are tested microorganisms. The methanol callus extract which grown on Murashige and Skoog (MS) media by using (Naphthalen acitic acid) NAA and (Benzyle adenine) BA as growth regulator highly effective as compared to the other extracts as for inhibition of three gram positive bacteria and three gram negative bacteria,which include Staphylococcus aureus and, Proteus vulgaris, followed by acetone and ethanolic extracts which include two gram positive bacteria and two gram negative bacteria. All extracts had highly effect in growth of Candida albicans while all crude extracts didn’t show any sensitivity against Saccharomyces boulardii, and when we’d done (High Performance Liquid Chromatography) HPLC test for detection of some active compound we found Quinic acid, Iridiods glycosides and Crocin which its rate in fresh callus was higher than fresh leaves.

scholarly journals Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria

2020 ◽  
Vol 16 (2) ◽  
Author(s):  
P. M. Ridzuan ◽  
Hairul Aini Hamzah ◽  
Anis Shah ◽  
Norazian Mohd Hassan ◽  
Baharudin Roesnita
Keyword(s):  
Pathogenic Bacteria ◽  
Synergistic Effects ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Synergistic Activity ◽  
Leaf Extracts ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Bacteria Growth ◽  
Gram Positive Bacteria

Antibacterial activity of different types of P. odorata leaf extracts was evaluated in combination with standard antibiotics. Persicaria. odorata leaves were extracted with n-hexane (n-hex), dichloromethane (DCM) and methanol (MeOH).  Each extract was applied on vancomycin (30µg), erythromycin (15µg) and gentamicin (10µg) discs, respectively. Disk diffusion method was used to evaluate the synergistic activity of each combination on Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli. Minimum inhibitory concentration (MIC) and gas chromatography mass spectrometry (GCMS) analysis was performed on the active extract. Synergistic effects seen were mainly from the n-hex+antibiotics combinations, mainly on the Gram-positive bacteria (7 additive, 5 antagonistic), with MIC range from 50 µg/ml to 100 µg/ml, as well as Gram-negative bacteria (2 additive, 2 indifferent, 5 antagonistic). In particular, synergism showed by the combination of n-hex+van were all additive against the susceptible bacteria. DCM extract combination showed synergistic effects on three Gram-positive species (S. aureus, S. epidermidis, S. pyogenes). Meanwhile, MeOH+antibiotics combination showed significant additive synergistic effects (p<0.05) on S. aureus and S. epidermidis.  The major compounds of leaves extract were decanal and β-citral. n-Hex extract superiorly inhibited Gram-positive bacteria growth as compared to DCM and MeOH extracts. The additive synergistic property of the n-hex P. odorata extract could be further studied for possible use as an antibacterial agent.

scholarly journals CnaA domains in bacterial pili are efficient dissipaters of large mechanical shocks

2016 ◽  
Vol 113 (9) ◽  
pp. 2490-2495 ◽  
Author(s):  
Daniel J. Echelman ◽  
Jorge Alegre-Cebollada ◽  
Carmen L. Badilla ◽  
Chungyu Chang ◽  
Hung Ton-That ◽  
...  
Keyword(s):  
Single Molecule ◽  
Pathogenic Bacteria ◽  
Mechanical Energy ◽  
Bacterial Attachment ◽  
Gram Positive ◽  
Covalent Bonds ◽  
Single Molecule Force Spectroscopy ◽  
Hydrophobic Collapse ◽  
Gram Positive Bacteria ◽  
Mechanical Shocks

Pathogenic bacteria adhere despite severe mechanical perturbations induced by the host, such as coughing. In Gram-positive bacteria, extracellular protein appendages termed pili are necessary for adherence under mechanical stress. However, little is known about the behavior of Gram-positive pili under force. Here, we demonstrate a mechanism by which Gram-positive pili are able to dissipate mechanical energy through mechanical unfolding and refolding of isopeptide bond-delimited polypeptide loops present in Ig-type CnaA domains. Using single-molecule force spectroscopy, we find that these loops of the pilus subunit SpaA of the SpaA-type pilus from Corynebacterium diphtheriae and FimA of the type 2 pilus from Actinomyces oris unfold and extend at forces that are the highest yet reported for globular proteins. Loop refolding is limited by the hydrophobic collapse of the polypeptide and occurs in milliseconds. Remarkably, both SpaA and FimA initially refold to mechanically weaker intermediates that recover strength with time or ligand binding. Based on the high force extensibility, CnaA-containing pili can dissipate ∼28-fold as much energy compared with their inextensible counterparts before reaching forces sufficient to cleave covalent bonds. We propose that efficient mechanical energy dissipation is key for sustained bacterial attachment against mechanical perturbations.

scholarly journals Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds

2011 ◽  
Vol 60 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Svetlana A. Ermolaeva ◽  
Alexander F. Varfolomeev ◽  
Marina Yu. Chernukha ◽  
Dmitry S. Yurov ◽  
Mikhail M. Vasiliev ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Plasma Treatment ◽  
Pathogenic Bacteria ◽  
Argon Plasma ◽  
Burkholderia Cenocepacia ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Plasma Treatments

Non-thermal (low-temperature) physical plasma is under intensive study as an alternative approach to control superficial wound and skin infections when the effectiveness of chemical agents is weak due to natural pathogen or biofilm resistance. The purpose of this study was to test the individual susceptibility of pathogenic bacteria to non-thermal argon plasma and to measure the effectiveness of plasma treatments against bacteria in biofilms and on wound surfaces. Overall, Gram-negative bacteria were more susceptible to plasma treatment than Gram-positive bacteria. For the Gram-negative bacteria Pseudomonas aeruginosa, Burkholderia cenocepacia and Escherichia coli, there were no survivors among the initial 105 c.f.u. after a 5 min plasma treatment. The susceptibility of Gram-positive bacteria was species- and strain-specific. Streptococcus pyogenes was the most resistant with 17 % survival of the initial 105 c.f.u. after a 5 min plasma treatment. Staphylococcus aureus had a strain-dependent resistance with 0 and 10 % survival from 105 c.f.u. of the Sa 78 and ATCC 6538 strains, respectively. Staphylococcus epidermidis and Enterococcus faecium had medium resistance. Non-ionized argon gas was not bactericidal. Biofilms partly protected bacteria, with the efficiency of protection dependent on biofilm thickness. Bacteria in deeper biofilm layers survived better after the plasma treatment. A rat model of a superficial slash wound infected with P. aeruginosa and the plasma-sensitive Staphylococcus aureus strain Sa 78 was used to assess the efficiency of argon plasma treatment. A 10 min treatment significantly reduced bacterial loads on the wound surface. A 5-day course of daily plasma treatments eliminated P. aeruginosa from the plasma-treated animals 2 days earlier than from the control ones. A statistically significant increase in the rate of wound closure was observed in plasma-treated animals after the third day of the course. Wound healing in plasma-treated animals slowed down after the course had been completed. Overall, the results show considerable potential for non-thermal argon plasma in eliminating pathogenic bacteria from biofilms and wound surfaces.

Antimicrobial Activity of Fractions and Compounds from Calophyllum brasiliense (Clusiaceae/Guttiferae)

2004 ◽  
Vol 59 (9-10) ◽  
pp. 657-662 ◽  
Author(s):  
Juliana B. Pretto ◽  
Valdir Cechinel-Filho ◽  
Vânia F. Noldin ◽  
Mara R. K. Sartori ◽  
Daniela E. B Isaias ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Protocatechuic Acid ◽  
Inhibitory Concentration ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts ◽  
Calophyllum Brasiliense

Abstract Calophyllum brasiliense (Clusiaceae/Guttiferae) is a native Brazilian medicinal plant traditionally used against several diseases, including infectious pathologies. Crude methanolic extracts (CME) and two fractions, denoted non-polar (soluble in chloroform) and polar (nonsoluble in chloroform), were prepared from different parts of the plant (roots, stems, leaves, flowers and fruits) and studied. The following compounds were isolated and tested against pathogenic bacteria and yeasts by determination of the minimal inhibitory concentration (MIC): brasiliensic acid (1), gallic acid (2), epicatechin (3), protocatechuic acid (4), friedelin (5) and 1,5-dihydroxyxanthone (6). The results indicated that all the parts of the plant exhibited antimicrobial activity against Gram-positive bacteria, which are selectively inhibited by components of C. brasiliense. No activity was observed against Gram-negative bacteria and yeasts tested. Regarding the isolated compounds, substance 4 showed antimicrobial activity against all the tested microorganisms, whereas compound 6 exhibited antimicrobial activity only against Gram-positive bacteria. The results from the current study confirm and justify the popular use of this plant to treat infectious processes.

scholarly journals FURTHER OBSERVATIONS ON THE GROWTH OF BACTERIA ON MEDIA CONTAINING VARIOUS ANILIN DYES, WITH SPECIAL REFERENCE TO AN ENRICHMENT METHOD FOR TYPHOID AND PARATYPHOID BACILLI

1914 ◽  
Vol 19 (5) ◽  
pp. 501-512 ◽  
Author(s):  
Charles Krumwiede ◽  
Josephine S. Pratt
Keyword(s):  
Special Reference ◽  
Pathogenic Bacteria ◽  
Negative Group ◽  
Enrichment Method ◽  
Gram Positive ◽  
Gram Negative ◽  
Selective Action ◽  
Gram Positive Bacteria ◽  
Growth Of Bacteria

Several green dyes show a marked selective action for members of the typhoid-paratyphoid-colon group. This can be used for the enrichment of typhoid and paratyphoid bacilli present in feces. Forty dyes were tested with thirty strains covering all types of pathogenic bacteria. In general the dyes restrained the growth of the Gram-positive bacteria but had no effect on the growth of the Gram-negative group.

scholarly journals Antibacterial Activities of Xanthium Strumarium L.

2018 ◽  
Vol 29 ◽  
pp. 70-77 ◽  
Author(s):  
Anjana Devkota ◽  
Ritu Kumari Das
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Xanthium Strumarium ◽  
Distilled Water ◽  
Leaf Extracts ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Antibacterial activities of Xanthium strumarium L. (Asteraceae) was carried out in laboratory. Distilled water and methanol extracts of the leaves of plant was prepared. The antibacterial activity was studied against six pathogenic bacteria, three gram negative: Klebsiella pneumoniae (ATCC 15380), Proteus mirabilis (ATCC 49132), Escherichia coli (ATCC 25922) and three gram positive: Bacillus subtilis (ATCC 6633), Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 25932) at different concentrations (50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ ml, 250 mg/ml) of leaf extracts of X. strumurium. The phytochemical screening depicted the presence of terpenoids, saponins, flavonoids, tannins and alkaloids. The antibacterial activity of extracts was determined by disc diffusion method and zone of inhibition (ZOI) was measured. Gram negative bacteria was found more resistant than gram positive bacteria. The most susceptible bacterium was S. aureus while the most resistant bacterium was E. coli. Methanolic extract was found more effective than distilled water. These findings suggest that extracts obtained from leaves of X. strumurium possess biobactericidal potential, which can suitably be exploited for making antibacterial drugs.J. Nat. Hist. Mus. Vol. 29, 2015, Page: 70-77

scholarly journals Potential Assessment of Rumex spp. as a Source of Bioactive Compounds and Biological Activity

2021 ◽  
Vol 12 (2) ◽  
pp. 1824-1834
Keyword(s):  
Antioxidant Activity ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
Gram Negative Bacteria ◽  
Meoh Extract ◽  
Gram Positive ◽  
Filter Paper Disc ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Antibacterial Performance

Secondary metabolites from the shoots and roots of three Rumex species collected from three different habitats were investigated (Rumex dentatus collected from cultivated land, R. pictus collected from the coastal desert and R. vesicarius collected from the inland desert) and tested for antioxidant activity as well as for anti-microbial activity against some human pathogenic bacteria. The present study indicated that the quantitative analysis of shoot and root extracts of three Rumex spp. were found to be rich in tannins and phenolics composition. The aerial parts of the three plants exhibited the highest significant values compared to the root parts. The MeOH extracts of Rumex species showed adequate antioxidant activity, wherein the IC50 values of the MeOH from the cultivated sample was 41.61 and 31.31 mg mL-1, coastal samples were 34.99 and 23.99 mg mL-1, while the sample of inland showed IC50 value of 41.59 and 31.67 mg mL-1, for root and shoot, respectively. Furthermore, using a filter paper disc assay, the MeOH extracts of the three Rumex species showed a substantial anti-microbial inhibitory effect on the growth of 10 pathogenic bacteria. According to sensitivity, the tested organisms could be sequenced as following: E. coli < K. pneumoniae ˂ S. typhi < P. aeruginosa for Gram-negative bacteria and B. subtilis < S. pneumoniae ˂ L. monocytoyenes < S. epidermis < S. aureus < B. cereus for Gram-positive bacteria. In addition, the antibacterial performance of R. dentatus root and R. vesicarius shoot MeOH extract is 100% broad spectrum against Gram-negative bacteria. A shoot of R. dentatus and R. pictus MeOH extract against Gram-positive bacteria is 83.3% broad spectrum. A further study is recommended for more characterization of the major compounds and assesses their efficiency and biosafety.

scholarly journals Penapisan Bakteri Filosfer Penghasil Senyawa Bioaktif Anti Xanthomonas oryzae pv. oryzae Penyebab Penyakit Hawar Daun Bakteri pada Padi

2016 ◽  
Vol 2 (1) ◽  
pp. 19-24
Author(s):  
NURFITRIANI RINA ◽  
NI PUTU RATNA AYU KRISHANTI ◽  
ALINA AKHDIYA ◽  
ARIS TRI WAHYUDI
Keyword(s):  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Luria Bertani ◽  
Xanthomonas Oryzae ◽  
Growth Stages ◽  
Pathogenicity Test ◽  
Bacterial Isolates ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Phyllosphere Bacteria

Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the important diseases in rice crops in Indonesia. The disease is difficult to be controlled since it attacks the rice plant at different growth stages such as tillering, flowering and ripening. One of the alternatives that could be used to control the disease is by using phyllosphere bacteria as the biocontrol agents. This study aims to isolate, characterize and screen the rice phyllosphere bacteria producing bioactive compounds against Xoo. Phyllosphere bacteria isolated from healthy leaves of rice var. Ciherang by using 4 different media obtained 285 bacterial isolates which were consisted of the 65 isolates of King’s B agar, 86 isolates of Nutrient agar, 81 isolates of Luria-Bertani agar, and 53 isolates of Trypticase Soy agar media. Antagonist test using double layer method showed 58 isolates of phyllosphere bacteria produced bioactive compounds that inhibited the growth of Xoo. Pathogenicity test agaist rice leaf revealed 18 bacterial isolates did not perform their potencies as pathogenic bacteria. Among the 18 non-phytopathogenic bacterial isolates, 14 isolates belong to Gram-positive bacteria and 4 isolates belong to Gram-negative bacteria. Five isolates among Gram positive bacteria were predicted as Bacillus genera. 

Antimicrobial Activity of Ethanolic and Watery Extracts to Fruit of Berberis vulagaris on growth of some species of pathogenic bacteria

2015 ◽  
Vol 9 (1) ◽  
pp. 87-81
Author(s):  
Rana Ali Hassan Hassan
Keyword(s):  
Hplc Analysis ◽  
Pathogenic Bacteria ◽  
High Ratio ◽  
Diffusion Method ◽  
High Concentration ◽  
Gram Positive ◽  
Alcohol Extract ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
High Effect

This study was done to test the activity of watery and alcohol extract of the fruit of Berberisvulagaris against of two species of gram positive bacteria Bacillus cereus and Staphylococcusaureus also ,two species of negative gram bacteria E. coli and Pseudomonas aeruginosa by agarwell diffusion method, the results showed that the alcohol extract of the Berberis vulagaris withhigh activity against gram positive and the effect was increase with the increasing of theconcentration. While the watery and alcohol extract has a high effect on gram negative bacteriawhich effect only with high concentration, while there is no effect in low concentration and theresults showed that the alcohol extract of the Berberis vulagaris with high activity against grampositive compare with watery extract. MIC and MBC were measured and the results wereidentical for the previous results, for the effect of both extracts on the growth of gram negativeand positive bacteria HPLC analysis was don for Berberis vulagaris which is quantitative andqualitative assay to measure the concentration of some alkaloid compound Tetrandrine,Chondocurine, bamine, which be with high ratio on Tetrandrine compound 38.42, the rate ofalkaloid compound in fruit of Berberis vulagaris was higher

Sign in / Sign up

Export Citation Format

Share Document