scholarly journals Analysis of Chemical Properties and Antioxidant Activity of Sambiloto (Andrographis paniculata Nees.) Leaf Tea Formula as a Functional Drink in Preventing Coronavirus Diseases and Degenerative Diseases

2021 ◽  
Vol 9 (A) ◽  
pp. 196-201
Author(s):  
Siti Ika Fitrasyah ◽  
Ariani Ariani ◽  
Nurdin Rahman ◽  
Nurulfuadi Nurulfuadi ◽  
Ummu Aiman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Chemical Properties ◽  
Water Soluble ◽  
National Standard ◽  
Water Extracts ◽  
Ash Alkalinity ◽  
Two Stages ◽  
Functional Drink

AIM: The purpose of this study was to develop a tea formula of sambiloto leaves, analyzed the chemical properties (polyphenols, tannins, water, extracts in water, total ash, water soluble ash, water-insoluble ash, insoluble acid ash, and water-soluble ash alkalinity) as well as the antioxidant capacity of the formula. METHODS: This research was divided into two stages. The first stage was the formulation of sambiloto leaves tea and the second stage was the analysis of chemical properties (levels of polyphenols, tannins, water, extracts water, total ash, water soluble ash, water-insoluble ash, insoluble acid ash, and water soluble ash alkalinity) and antioxidant capacity analysis. The analysis was carried out on all of the sambiloto leaves tea formulas to determine the effect of the formulations on chemical properties and antioxidant capacity. This study used Microsoft Excel 2013 in data processing management. RESULTS: The highest extract water was at F4, and the lowest was at F1. Based on the analysis, the formula of sambiloto leaves tea was in the range of 1.026–1.734%. Of all the formulas that have been prepared, sambiloto leaves tea formula F1 was obtained the best chemical and antioxidant properties among all formulas, which were composed of 100% sambiloto leaves, 5% honey, and 45% dates. CONCLUSION: Sambiloto leaves have good antioxidant activity and can be used as a drink in the form of an infusion, such as tea. The result of brewing the sambiloto tea formula has faded green color. The polyphenol content of the sambiloto leaves tea formula still did not meet the Indonesia National Standard (≥5.2%), was 1.5–3.1%.

Antioxidant Capacities of Water-Soluble Substances Extracted from JinHua Ham

2014 ◽  
Vol 675-677 ◽  
pp. 1654-1657
Author(s):  
Xiao Li Zhou ◽  
Xu Chen ◽  
Ting Feng Hao ◽  
Yi Ming Zhou ◽  
Ying Xiao
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Mass Fraction ◽  
Water Soluble ◽  
Oxidation Capacity ◽  
Hydroxyl Free Radical ◽  
Antioxidant Capacities ◽  
Dpph Scavenging ◽  
Jinhua Ham

This study was designed to evaluate the antioxidant activity of samples extracted from Jinhua ham by using such chemical assays as DPPH, scavenging hydroxyl free radical and ABTS. The results demonstrate that antioxidant capacity of the extracted sample is lower than oxidation capacity of Vc. The IC50 spot of samples ranges between 0.6 % and 2.5 % (mass fraction of solute).

scholarly journals Antioxidant properties of hot water extracts from carpophore and spores of mushroom Ganoderma lucidum

2011 ◽  
pp. 279-288 ◽  
Author(s):  
Anita Klaus ◽  
Maja Kozarski ◽  
Miomir Niksic
Keyword(s):  
Antioxidant Activity ◽  
Ganoderma Lucidum ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Reducing Power ◽  
Hot Water ◽  
Ferrous Ions ◽  
Medicinal Mushrooms ◽  
Water Extracts ◽  
Hot Water Extract

Ganoderma lucidum (Leyss.:Fr.) Karst is one of the medicinal mushrooms, which possesses enviable antioxidant properties. Objective of this investigation was to evaluate antioxidant activity, reducing power, scavenging abilities on 1.1-diphenyl-2-picrylhydrazyl (DPPH) radicals and chelating effects on ferrous ions of hot water extracts obtained from carpophore and spores of this mushroom. Hot water extract from carpophore (Gl-I) showed high antioxidant activity of 85.7 ? 0.7%, at 10 mg/ml, while antioxidant activity of hot water extract from spores (Gl-Is) was 9.2 ? 0.3% at 10 mg/ml. Reducing power of Gl-I reached a plateau of 3.4 ? 0.1 at 20 mg/ml, and 0.3 ? 0.0 at 20 mg/ml for Gl-Is. At 10 mg/ml, scavenging ability on DPPH radicals of Gl-I increased to 96.8 ? 2.5%, whereas Gl-Is scavenged DPPH radicals by 69.6 ? 2.5% at 10 mg/ml. Gl-I chelated 81.6 ? 3.6 % of ferrous ions at 20 mg/ml, while the chelating effect of Gl-Is was 73.8 ? 1.7%. The antioxidative activities of hot water extracts from carpophore and spores of the mushroom G. lucidum were concentration dependent and increased with an increase in the concentration.

scholarly journals Pelargonium graveolens Aqueous Decoction: A New Water-Soluble Polysaccharide and Antioxidant-Rich Extract

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Malek Ennaifer ◽  
Taroub Bouzaiene ◽  
Moncef Chouaibi ◽  
Moktar Hamdi
Keyword(s):  
Antioxidant Activity ◽  
Flow Behavior ◽  
Chemical Properties ◽  
Extraction Yield ◽  
Water Soluble ◽  
Pelargonium Graveolens ◽  
Parameters Extraction ◽  
Soluble Polysaccharide ◽  
Dpph Scavenging ◽  
The One

Background. The decoction of Pelargonium graveolens yields an antioxidant-rich extract and a water-soluble polysaccharide. This study aims (1) to investigate the effect of process parameters (extraction time and temperature) on the antioxidant activity of the decoction and the extraction yield of CPGP by response methodology and (2) to study the chemical properties of the optimized decoction and rheological properties of the corresponding extracted polysaccharide. Results. The antioxidant-rich decoction contained about 19.76 ± 0.41 mg RE/g DM of flavonoids and 5.31 ± 0.56 mg CE/gDM of condensed tannins. The crude Pelargonium graveolens polysaccharide (CPGP) contained 87.27 % of sugar. Furthermore, the CPGP solutions (0.5%, 1%, and 2%) exhibited shear-thinning or pseudoplastic flow behavior. A central composite design (CDD) was applied to assess the effects of temperature and time on the antioxidant activity of the decoction, on the one hand, and on water-soluble polysaccharide yield, on the other. The decoction optimization of Pelargonium graveolens aimed to use less energy (93°C for 11 minutes) leading to the highest values of decoction phenolic content (33.01 ±0.49 mg GAE/gDM) and DPPH scavenging activity (136.10 ± 0.62 mg TXE/gDM) and the highest values of CPGP yield (6.97%). Conclusion. The obtained results suggest that the CPGP rheological characteristics are suitable for applications in many industries, especially food. The values of optimal conditions showed that Pelargonium graveolens decoction operation could have multiple uses, especially for consuming less energy.

scholarly journals The presence of endophytic actinobacteria in mangosteen peel (Garcinia mangostana) and its antioxidant activity

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Fily Larasati ◽  
IRMANIDA BATUBARA ◽  
YULIN LESTARI
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Rrna Gene ◽  
Blue Color ◽  
Garcinia Mangostana ◽  
Mangosteen Peel ◽  
Endophytic Actinobacteria

Abstract. Larasati F, Batubara I, Lestari Y. 2020. The presence of endophytic actinobacteria in mangosteen peel (Garcinia mangostana L.) and its antioxidant activity. Biodiversitas 21: 1488-1497. Mangosteen (Garcinia mangostana L.) is a family member of Clusiaceae which is rich in secondary metabolite compounds that can function as antioxidants. Besides being produced by its host plant, the bioactive compounds can also be produced by endophytic actinobacteria. The purpose of this study was to explore the presence of endophytic actinobacteria from mangosteen peel and determine its antioxidant activity. The actinobacteria were isolated, purified, morphologically characterized, molecularly identified, extracted with ethyl acetate and tested for antioxidant properties. The antioxidant activity was assayed using DPPH (2.2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) methods. The components of extracts were separated by Thin Layer Chromatography (TLC) and bioautography was done to determine the antioxidant bands. As a result, five isolates of endophytic actinobacteria in mangosteen peel showed to have difference in aerial mycelium color, substrate mycelium color, and types of spore chains. Based on 16S rRNA gene analysis, AGM3.2 isolate showed similarity with Streptomyces griseochromogenes ATCC 14511 (T) 99.06%. AGM3.1 had similarity with Streptomyces osmaniensis OU-63 (T) 98.35%. Meanwhile, AGM2.3 were similar to Streptomyces xanthophaeus NBRC B-5414 (T) 99.82%, AGM2.2 had similarity with Streptomyces xanthophaeus NBRC B-5414 (T) 98.95%. In addition, AGM2.1 has homology with Streptomyces goshikiensis NBRC 12868 (T) 99.52%. Using both DPPH and ABTS, supernatant of AGM2.1 showed the highest antioxidant activity indicated by 36.96 and 98.80 inhibition, respectively. Antioxidant capacity of ethyl acetate extract of AGM2.1 was 22.22 μg AEAC/mg extract (DPPH) and 20.34 μg AEAC/mg extract (ABTS). Meanwhile, ethyl acetate extract of mangosteen peel had antioxidant capacity by 21.17 µg AEAC/mg extract (DPPH) and 18.75 µg AEAC/mg extract (ABTS). Antioxidant bioautographic analysis of mangosteen peel ethyl acetate extract was compared with alpha mangosteen standard. The results showed that alpha mangosteen presence in the mangosteen extract with the same Rf value of 0.64 with standard. Meanwhile, actinobacterial ethyl acetate extract from AGM3.1, AGM2.3, AGM2.2, AGM2.1 each have the same Rf value with the alpha mangosteen standard. However, the spot for alpha mangosteen had dark red color, while spots of the four actinobacterial isolates showed to have blue color indicating different antioxidant compounds. The blue spot indicates the flavone, flavanone, flavonol, and isoflavone. These compounds include a subgroup of flavonoid compounds. Ethyl acetate extract AGM3.2 does not have spot compounds with the same Rf value as the alpha mangosteen standard. Study clearly shows that endophytic actinobacteria from mangosteen peel have potency as antioxidant.

scholarly journals Phytochemical Diversity in Rhizomes of Three Reynoutria Species and their Antioxidant Activity Correlations Elucidated by LC-ESI-MS/MS Analysis

2019 ◽  
Author(s):  
Izabela Nawrot-Hadzik ◽  
Sylwester Ślusarczyk ◽  
Sebastian Granica ◽  
Jakub Hadzik ◽  
Adam Matkowski
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Therapeutic Effects ◽  
Similar Species ◽  
Reynoutria Japonica ◽  
Esi Ms ◽  
Total Antioxidant ◽  
First Time

One of the richest natural sources of resveratrol - the rhizome of Reynoutria japonica in East Asia is a well-known traditional herb (Hu zhang) used in various inflammatory diseases, infections, skin diseases, scald, hyperlipidemia. Although, it has been recently included in the European Pharmacopoeia, still in Europe is an untapped resource. Some of the therapeutic effects are likely to be influenced by its antioxidant properties and this in turn is frequently associated with a high stilbene content. However, some literatures suggested that other compounds than stilbenes may add to the total antioxidant capacity. Hence, the aim of this research was to examine rhizomes of R. japonica and less studied, morphologically similar species, R. sachalinensis and R. x bohemica for their phytochemical composition and antioxidant activity and to clarify the relationship between the antioxidant activity and compounds by statistical methods. HPLC/UV/ESI-MS studies of three Reynoutria species revealed 171 compounds comprising stilbenes, carbohydrates, procyanidins, flavan-3-ols, anthraquinones, phenylpropanoids, lignin oligomers, hydroxycinnamic acids, naphthalenes and their derivatives. Our studies confirmed the presence of procyanidins with high degree of polymerization, up to decamers in the rhizomes of R. japonica and brings new data on the presence of these compounds in other Reynoutria species. A procyanidin trimer digallate was described for the first time in the studied plants. Moreover, we suggested a presence of new for these species, dianthrone glycosides and previously unrecorded phenylpropanoid disaccharide esters and hydroxycinnamic acid derivatives, mainly in R. sachalinensis. Furthemore, compounds tentatively annotated as lignin oligomers were observed for the first time in studied species. The rhizomes of all Reynoutria species exhibited strong antioxidant activity. Statistical analysis demonstrated that proanthocyanidins should be considered as important contributors to the total antioxidant capacity.

scholarly journals Antioxidant Activity and Combination Characteristics of Filtrates and Sargassum polycystum Seaweed Salt Residue

2021 ◽  
Author(s):  
Nurjanah Nurjanah ◽  
◽  
Asadatun Abdullah ◽  
Amalia Rahmadhani ◽  
Anggrei Viona Seulalae ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Dietary Fiber ◽  
Brown Seaweed ◽  
Fiber Content ◽  
Raw Material ◽  
National Standard ◽  
High Yield ◽  
Dietary Salt ◽  
Sargassum Polycystum

Sargassum polycystum is a low-sodium salt raw material that met the dietary salt criteria, i.e., low Na: K ratio and NaCl content < 60%. This study aims to produce seaweed salt with high yield, dietary fiber content, and antioxidant activity according to the quality standard of dietary salt through a combination of the filtrate and salt extraction residue. The results showed that the produced S. polycystum brown seaweed salt had a mineral Na 96.97 mg/g and mineral K 247.59 mg/g. Na: K ratio of 0.39, NaCl content of 49.05%, and heavy metal residue below the Indonesian National Standard maximum standard for dietary salt. The levels of dietary fiber produced in salt samples 1:1, 1:2, and 2:1 were 34.41%, 40.16%, and 23.83%, respectively, and the iodine content (KIO3) of the control salt was 125.95 mg/kg. The antioxidant activity of seaweed salt 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) on salt K (seaweed salt without residue), residue, S1 (1:1), S2 (1:2) and S3 (2:1) was 26.41 ppm, 59.06 ppm, 12.29 ppm, 67.30 ppm and 87.50 ppm. Meanwhile, the antioxidant capacity of seaweed salt Cupric Reducing Antioxidant Capacity (CUPRAC) on K salt, residue, S1, S2 and S3 were 12.36, 19, 7.86, 8.52 and 12.27 μmol ascorbic acid/g extract, respectively. Therefore, it was concluded that increasing the yield and dietary fiber content in the seaweed salt production process is achievable by adding residues.

scholarly journals In vitro antioxidant activity of meniran (Phyllantus urinaria) functional drink in human low density lipoprotein (LDL)

2021 ◽  
Vol 913 (1) ◽  
pp. 012093
Author(s):  
U Fitrotin ◽  
N Hilmiati ◽  
Mardiana ◽  
Y Triguna ◽  
A Surahman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Density Lipoprotein ◽  
Ldl Oxidation ◽  
Functional Drink

Abstract Preparation process for meniran (Phillantus urinaria) functional drink (MFD) influences its antioxidant activity. This research aims to understand the phenolic content, DPPH Radical Scavenging Activity (RSA), and LDL oxidation of MFD through various preparation processes. Those preparation processes included soaking fresh meniran (SFM), boiling fresh meniran for 5 minutes (BFM5’), boiling fresh meniran for 10 minutes (BFM10’), and soaking dried meniran (DM). The phenolic content was determined with Folin–Ciocalteu, antioxidant activity was assessed using DPPH and TBARS assay with LDL as the oxidation substrate. An antioxidant references in this research used ascorbic acid. The phenolic content in methods of SFM, BFM5’, BFM10’ and DM were 122±0.022, 182±0.043, 192 ±0.03, and 117 ±0.019 mg GAE/g of meniran respectively. Meanwhile, the DPPH RSA of SFM, BFM5’, BFM10’ and DM accounted for 82.18±0.35, 86.19±0.53, 86.75±0.64 and 69.96% respectively. As comparison, the DPPH RSA of ascorbic acid 50 ppm is 75.65±0.82%. At the same time the optimum inhibition of TBARS formation from BFM5’ and BFM10’ methods were 45.83 % and 48.66%, with MDA concentration in human LDL accounted for 38.30±2.39 and 36.30±1.82 nmol MDA/mg protein, respectively. As comparison, MDA concentration in human LDL added with ascorbic acid 25 ppm accounted for 41.35±2.41 nmol MDA/mg protein. In contrast, the control human LDL was 70.70±2.35 nmol MDA/mg protein. This study concludes that the BFM5’ and BFM10’ methods showed the highest antioxidant properties compared to other methods. All methods showed that MFD extract in concentration more than 25 ppm increased the concentration of MDA in human LDL. Therefore, to produce meniran functional drink in optimum antioxidant properties is best by using BFM5’ and BFM10’ preparation methods in meniran concentration of not more than 25 ppm.

Influence of thermal modification and extraction techniques on yield, antioxidant capacity and phytochemical profile of chestnut (Castanea sativa Mill.) wood

Holzforschung ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Immacolata Faraone ◽  
Daniela Russo ◽  
Maurizio D’Auria ◽  
Maria Roberta Bruno ◽  
Paola Cetera ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Castanea Sativa ◽  
Total Content ◽  
Thermal Modification ◽  
Gas Chromatography Mass Spectrometry ◽  
Antioxidant Compounds ◽  
Extraction Techniques ◽  
Ultrasound Assisted Extraction

AbstractNumerous investigations on the antioxidant properties of different plant tissue extracts are available in literature, but few studies on the effect of thermally modified wood on secondary metabolites were carried out up to now. This study highlights the influence of the thermal modification of Castanea sativa Mill. wood on total content of antioxidant compounds and consequently on its antioxidant activity. In addition, a chemical profile by Gas Chromatography–Mass Spectrometry (GC–MS) of the extractives was carried out. Wood of chestnut, thermally modified at 180 °C for 3 h, was used to obtain wood meal which was subjected to different extraction techniques, as maceration extraction (ME), ultrasound assisted extraction (UAE) and accelerated solvent extraction (ASE). The total content of principal antioxidant compounds, such as polyphenols, flavonoids and tannins, as well as the evaluation of antioxidant capacity by using different in vitro assays were determined. Relative Antioxidant Capacity Index (RACI), which is used to compare all antioxidant parameters, has also been applied. The study demonstrated a positive influence on chemical compounds present in C. sativa Mill wood originating from the thermal modification process. Thus, is possible to consider thermal modification as a promising strategy to improve the antioxidant activity of chestnut wood extractives.

scholarly journals ANTIOXIDANT ACTIVITIES AND TOTAL PHENOLIC AND FLAVONOID CONTENT VARIATIONS OF LEAF EXTRACTS OF LAURUS NOBILIS L. FROM MOROCCO

2018 ◽  
Vol 11 (12) ◽  
pp. 540 ◽  
Author(s):  
Amal Taroq ◽  
Fatima El Kamari ◽  
Imane Aouam ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Laurus Nobilis ◽  
Dry Extract ◽  
Water Extracts ◽  
Phenolic And Flavonoid

Objective: The present study was undertaken to determine antioxidant activity and total phenolic and flavonoid content of ethanol, methanol, ethyl acetate, and aqueous extracts of the leaves of Laurus nobilis L.Methods: Antioxidant properties were measured using three tests: Free radical scavenging activity against 2,2-diphenyl picrylhydrazyl (DPPH), reduction of molybdate, and reducing (Fe3+/Fe2+) power. Total phenolic and flavonoid content was measured by Folin–Ciocalteu and rutin reagent, respectively.Results: The results showed that both the ethanol and water extracts had greater antioxidant activity than ethyl acetate. Aqueous extract exhibited a higher DPPH radical scavenging and reducing molybdate 545.83±5.89 mg equivalent to ascorbic acid/g dry extract. The strong antioxidant activity of water extract was probably due to its high content of phenols. Furthermore, ethanol and water extracts showed higher total flavonoid content with the values: 153.33±3.59 and 127.25±2.60 mg equivalent rutin/g dry extract, respectively.Conclusion: Our results suggested a potent and excellent antioxidant activity of L. nobilis L. extracts of Morocco. Further research has to be done to isolate the pure bioactive compound and could be considered as a potential source of biomolecules for pharmaceutical and food industry.

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