scholarly journals The Role of Vitamin C in Amelioration of Hepatorenal Toxicity of Cefotaxime in Adult Albino Rats (Histological Study)

2021 ◽  
Vol 9 (A) ◽  
pp. 845-848
Author(s):  
Maha Al Sammak ◽  
Rana M. Ahmed ◽  
Nadwa Alazzo
Keyword(s):  
Vitamin C ◽  
Histological Study ◽  
Inflammatory Cells ◽  
Control Group ◽  
Albino Rats ◽  
Renal Tubules ◽  
Male Adult ◽  
Liver And Kidney ◽  
Group 2

AIM: Antibiotics have a great risk property, for this reason, the present work aimed to study the toxic effect of cefotaxime on histological examination of liver and kidney tissues as well as to detect the protecting role of Vitamin C. METHODS: Thirty-two male adult albino rats were divided into four groups each with (eight animals) as following: Group (1): As control group and they injected with normal saline. Group (2): They were injected with 200 mg/kg B.W. of cefotaxime. Group (3): They were injected with Vitamin C in dose 100 mg/kg B.W. 1 h before they inject with 200 mg/kg B.W. of cefotaxime. Group (4): It was given Vitamin C in dose of 100 mg/kg B.W. Animals in all groups were injected intraperitoneally as single daily dose for 14 consecutive days. RESULTS: Results of cefotaxime treated group revealed that a significant liver tissue changes as hepatocytic vacuolation, necrosis, cholestasis with sinusoidal congestion, and dilatation also induced a histopathological change in the kidney including tubular epithelial degeneration, cast formation in renal tubules, inflammatory cells infiltration in the interstitium, and few glomeruli showed eosinophilic material deposition at the wall of bowman capsule. Adding Vitamin C to third group induces amelioration in the histological features of liver and kidney seen in Group (2) while group of Vitamin C only showed a histological picture similar to control group. CONCLUSION: From this study, we can conclude that Vitamin C has important hepato-renal protective effect.

Effects of erdosteine on cyclosporin-A-induced nephrotoxicity

2011 ◽  
Vol 31 (6) ◽  
pp. 565-573 ◽  
Author(s):  
M Tutanc ◽  
V Arica ◽  
N Yılmaz ◽  
A Nacar ◽  
I Zararsiz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cyclosporin A ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Protective Mechanism ◽  
Antioxidant Parameters ◽  
Group 2 ◽  
Wistar Albino Rats

Aim: In cyclosporin-A (CsA)-induced toxicity, oxidative stress has been implicated as a potential responsible mechanism. Therefore, we aimed to investigate the protective role of erdosteine against CsA-induced nephrotoxicity in terms of tissue oxidant/antioxidant parameters and light microscopy in rats. Materials and methods: Wistar albino rats were randomly separated into four groups. Group 1 rats treated with sodium chloride served as the control, group 2 rats were treated with CsA, group 3 with CsA plus erdosteine, and group 4 with erdosteine alone. Animals were killed and blood samples were analyzed for blood urea nitrogen (BUN), serum creatinine (Cr), uric acid (UA), total protein (TP), and albumin (ALB) levels. Kidney sections were analyzed for malondialdehyde (MDA) and nitric oxide (NO) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities, as well as for histopathological changes. Results: In the CsA group, MDA, GSH-Px, BUN, and Cr levels were increased. The TP and ALB levels were decreased. These changes had been improved by erdosteine administration. Other biochemical parameters did not show any significant change. Conclusion: These results indicate that erdosteine produces a protective mechanism against CsA-induced nephrotoxicity and suggest a role of oxidative stress in pathogenesis.

scholarly journals Vitamin C attenuates methotrexate-induced oxidative stress in kidney and liver of rats

2017 ◽  
Vol 104 (2) ◽  
pp. 139-149 ◽  
Author(s):  
M Savran ◽  
E Cicek ◽  
DK Doguc ◽  
H Asci ◽  
S Yesilot ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Histopathological Examination ◽  
Redox System ◽  
Protective Role ◽  
Hepatic Tissue ◽  
Control Group ◽  
Liver And Kidney ◽  
Vit C

Like several other anticancer drugs, methotrexate (MTX) causes side effects, such as neuropathic pain, hepatotoxicity, and nephrotoxicity. Abnormal production of reactive oxygen species has been suspected in the pathophysiology of MTX-induced hepatorenal toxicity. Therefore, the aim of this study was to investigate the probable protective role of vitamin C (Vit C) on oxidative stress induced by MTX in the liver and kidney tissues of rats. A total of 32 rats were randomly and equally divided into four groups. The first group served as the control group. The second group received a single dose of 20 mg/kg of MTX intraperitoneally. To demonstrate our hypothesis, the third and the fourth groups received 250 mg/kg of Vit C for 3 days by oral gavage, with or without MTX treatment. At the end of the study, the liver and kidney tissues of the rats were collected and examined using histology. Both the tissues were assayed for malondialdehyde concentration and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities. In hepatic and renal tissues, lipid peroxidation levels were increased, whereas SOD, CAT, and GSH-Px levels were decreased by MTX. All parameters, including CAT levels in hepatic tissue, were significantly restored after the administration of Vit C for 3 days. Similar to the biochemical findings, evidence of oxidative damage was examined in both types of tissues by histopathological examination. From the results of this study, we were able to observe that Vit C administration modulates the antioxidant redox system and reduces the renal and hepatic oxidative stress induced by MTX. Vit C can ameliorate the toxic effect of MTX in liver and kidney tissues of rat.

scholarly journals Protective Role of Rockect Seed (Eruca sativa) Extract against Monosodium Glutamate-induced Hepato-renal Toxicity in Male Rats

2020 ◽  
pp. 1-10
Author(s):  
Ehab Tousson ◽  
Afaf El-Atrash ◽  
Yosra Karson
Keyword(s):  
Renal Damage ◽  
Renal Toxicity ◽  
Monosodium Glutamate ◽  
Chloride Ions ◽  
Male Rats ◽  
Male Rat ◽  
Albino Rats ◽  
Male Adult ◽  
Liver And Kidney

Background and Objective: Monosodium glutamate (MSG) is identified as an Accent that is used in the food industry as a flavour enhancer with an umami taste that intensifies the meaty, savoury flavour of food. The present study aimed at evaluating the protective and ameliorative role of rocket seeds extract against monosodium glutamate-induced hepatic renal toxicity and oxidative stress in the male rat. Materials and Methods: A total of 60 male adult albino rats were equally divided into six groups (G1, Control; G2, rocket seeds (RS); G3, ACCENT or MSG; G4, Co- treated (RS+MSG); G5, Post- treated (MSG+RS); G6, Self-treated MSG).  Results: Current results revealed that; a significant increase in serum ALT, AST, ALP, AFP, Urea, Creatinine, potassium ions, chloride ions, cholesterol, triglyceride, HDL, and LDL levels in MSG as compared to control and RS groups. In contrast; a significant decrease in serum albumin, total proteins, catalase, GSH and SOD in liver and kidney homogenates in MSG as compared to control and RS groups. Co- or post-treatment of MSG with rocket seeds improved this change in liver and kidney functions, with best results for co-treatment than post and self-treatment. Conclusion: These findings suggested that the misuse of monosodium glutamate may contribute to continuous hepatic and renal damage. This shows that the desired dose of monosodium glutamate can safely be used with grapes seed in improving hepatic and renal damage in monosodium glutamate in young rats.

scholarly journals Toxicopathological and Molecular Studies on Imidacloprid and Hexaflumuron-induced Hepatorenal Toxicity in Rats.

2021 ◽  
Author(s):  
Eman I. Hassanen ◽  
Ahmed M. Hussien ◽  
Sally Mehanna ◽  
Marwa A. Ibrahim ◽  
Neven H. Hassan
Keyword(s):  
Caspase 3 ◽  
Crop Protection ◽  
Serum Levels ◽  
Saline Group ◽  
Control Group ◽  
Albino Rats ◽  
Behavioral Alterations ◽  
Apoptosis Pathway ◽  
Liver And Kidney ◽  
Group 2

Abstract Pesticides are considered the main source of environmental pollution and causing severe hazardous effects on humans and livestock. Imidacloprid (IC) and hexaflumuron (HFM) are broadly used insecticides for crop protection in the world. Some studies discussed IC toxicity in rats, but the toxicity of HFM doesn’t elucidate yet. So that, the current study aimed to investigate the pathogenesis and the mechanistic way of both IC and HFM induced hepatorenal toxicity in rats with comprehensive insight into its molecular mechanism. 21 male Wistar albino rats were divided into 3 groups as the following: group (1), normal saline; group (2), receiving IC; and group (3), receiving HFM. All the following materials were orally administered every day for 28 days. At the end, all rats were euthanized to collect blood and organ samples (liver and kidneys). The results revealed behavioral alterations in walking, body tension, alertness, and head movement as well as a decrease in body weight of rats receiving either IC or HFM. In addition to increasing the levels of MDA with decreasing GHS levels in liver and kidney homogenates. Both liver and kidney tissues showed extensive histopathological alterations associated with increasing the serum levels of ALT, AST, urea, and creatinine as well as reduction in total proteins, albumin, and globulin levels. Furthermore, there was upregulation of m-RNA levels of caspase-3, JNK, and HO-1 genes with strong positive reaction of caspase-3, TNF-ὰ and NF-KB proteins in both liver and kidneys of rats receiving either IC or HFM compared with the control group. We can conclude that both IC and HFM induced oxidative hepatorenal damage via ROS overproduction that activate NF-KB signaling pathways and mitochondrial and JNK dependent apoptosis pathway.

scholarly journals Comparative assessment on the probable mechanisms underlying the hepatorenal toxicity of commercial imidacloprid and hexaflumuron formulations in rats

2022 ◽  
Author(s):  
Eman I. Hassanen ◽  
Ahmed M. Hussien ◽  
Sally Mehanna ◽  
Marwa A. Ibrahim ◽  
Neven H. Hassan
Keyword(s):  
Kidney Tissue ◽  
Serum Levels ◽  
Saline Group ◽  
Control Group ◽  
Albino Rats ◽  
Apoptosis Pathway ◽  
Liver And Kidney ◽  
Group 2 ◽  
Wistar Albino Rats ◽  
Tissue Specimens

Abstract Pesticides are viewed as a major wellspring of ecological contamination and causing serious risky consequences for people and animals. Imidacloprid (IM) and hexaflumuron (HFM) are extensively utilized insect poisons for crop assurance on the planet. A few investigations examined IM harmfulness in rodents, but its exact mechanism hasn’t been mentioned previously as well as the toxicity of HFM doesn’t elucidate yet. For this reason, the present study was designed to explore the mechanism of each IM and HFM–evoked rat liver and kidney toxicity and to understand its molecular mechanism. 21 male Wistar albino rats were divided into 3 groups, as follows: group (1), normal saline; group (2), IM; and group (3), HFM. Both insecticides were orally administered every day for 28 days at a dose equal to 1/10 LD50 from the active ingredient. After 28 days postdosing, rats were anesthetized to collect blood samples then euthanized to collect liver and kidney tissue specimens. The results showed marked changes in walking, body tension, alertness, and head movement with a significant reduction in rats’ body weight in both IM and HFM receiving groups. Significant increases in MDA levels and decrease of GHS levels were recorded in liver and kidney homogenates of either IM or HFM groups. Liver and kidney tissues obtained from both pesticide receiving groups showed extensive histopathological alterations with a significant increase in the serum levels of ALT, AST, urea, and creatinine and a decrease in total proteins, albumin, and globulin levels. In addition, there was upregulation of the transcript levels of casp-3, JNK, and HO-1 genes with strong immunopositivity of casp-3, TNF-ὰ, and NF-KB protein expressions in the liver and kidneys of rats receiving either IM or HFM compared with the control group. In all studied parameters, HFM caused hepatorenal toxicity more than those induced by IM. We can conclude that each IM and HFM provoked liver and kidneys damage through overproduction of ROS, activation of NF-KB signaling pathways and mitochondrial/JNK-dependent apoptosis pathway.

scholarly journals AMELIORATIVE EFFECT OF BERBERIS VULGARIS FRUIT EXTRACT AGAINST GENTAMICIN INDUCED NEPHROTOXICITY IN ALBINO RATS

2021 ◽  
Vol 71 (2) ◽  
pp. 676-80
Author(s):  
Lubna Faisal ◽  
Zia -Ul- Islam ◽  
Saima Athar ◽  
Sadia Iqbal ◽  
```Fatima Rehman ◽  
...  
Keyword(s):  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Fruit Extract ◽  
Male Adult ◽  
Berberis Vulgaris ◽  
Group B ◽  
Group D ◽  
Control Group Group

Objective: To observe the nephroprotective role of berberis vulgar is on renal parenchyma against microscopic and morphometric changes induced by Gentamicin in albino rats. Study Design: Lab based experimental study. Place and Duration of Study: It was conducted in Baqai Medical University in collaboration with department of Anatomy Liaquat National Hospital and Medical College, from Jan to Jul 2017. Methodology: A total of 40 male adult albino rats were used in the study. Four groups were made. Each group contained 10 rats. Group A was a control group, group B received only berberis vulgaris fruit extract orally per day for 21 days, group C received Gentamicin 100 mg/kg/day intraperitonially daily for 21 days. Group D received Gentamicin 100 mg/kg/day intraperitonially along with berberis vulgaris fruit extract 100 mg/kg/day orally. Both kidneys were removed. H&E and PAS stains were used for observing histological alterations and protective role of berberis vulgaris fruit extract. Results: Glomerulus and proximal convoluted tubules were observed histologically in all 4 groups. Microscopy of group B showed parameters nearly similar to control group. Microscopy of group C showed significant derangement in all parameters when compared with control group. Group C showed decrease glomerular, proximal convoluted tubular count was noted. Glomerular diameter increases and there was glomerular hypertrophy and tubular necrosis. Microscopy of group D showed significant improvement due to berberis vulgaris which restored normal renal architecture. Conclusion: Berberis vulgaris has a nephroprotective effect and it can be used as a new medicine against nephrotoxic drugs like Gentamicin.

Citrullus colocynthis Linn. Fruit extract ameliorates cisplatin-induced hepato-renal toxicity in rats

2017 ◽  
Vol 15 (1) ◽  
Author(s):  
Olufunmilayo O. Adeyemi ◽  
Ismail O. Ishola ◽  
Ifeoluwa D. Ajani
Keyword(s):  
Histological Analysis ◽  
Renal Toxicity ◽  
Serum Levels ◽  
Control Group ◽  
Albino Rats ◽  
Citrullus Colocynthis ◽  
Fruit Extract ◽  
Liver And Kidney ◽  
Group 2 ◽  
Group 1

AbstractBackgroundCisplatin-induced acute liver and kidney injuries are serious problems in cancer patients during treatment of solid tumours.ObjectiveThis study sought to investigate possible protective effect of ethanolic fruit extract ofMethodsThirty male albino rats (150–200 g) were divided into five groups (n=6) and treated as follows: group 1: vehicle (10 mL/kg, p.o.; normal control); group 2: vehicle (10 mL/kg); groups 3–5: CC (100, 200 or 400 mg/kg, p.o.), respectively, for 10 days. Cisplatin (7.5 mg/kg; i.p.) was administered on the 7th day to animals in groups (2–5) 1 h after pretreatment. The animals were euthanized on day 10 for haematological, biochemical and histological analysis.ResultsCisplatin induced a significant increase in the serum levels of ALT, ALP, creatinine and blood urea nitrogen indicative of hepato-renal injury. More so, cisplatin caused marked increase in granulocyte, lymphocyte and platelets counts which were ameliorated by CC (100–400 mg/kg) treatment. In addition, cisplatin induced marked increase in MDA and nitrite levels coupled with deficits in glutathione, catalase and superoxide dismutase activities which were attenuated by CC administration.ConclusionsThis study showed that

scholarly journals Comparative histophysiological study the effective treatment of induced eczema in rats that treated with Lanolin and glycerine with Vaseline in rats

2020 ◽  
Vol 11 (2) ◽  
pp. 63-69
Author(s):  
Dher Al-Haidary ◽  
◽  
Mohammad Naqi
Keyword(s):  
Skin Color ◽  
Inflammatory Cells ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Changes ◽  
Sebaceous Glands ◽  
Control Section ◽  
Oily Product ◽  
Pure Acetone

The present study has been designed to evaluate the potent role of healing efficacy of lanolin (which is also called wool wax), is an oily product secreted by the sebaceous glands beneath the skin of sheep which is have anti-inflammatory, anti-microbial, skin protecting properties, a forty-eight mature albino rats (weighted 250-350 g) were randomly assigned to two groups; control group and eczema group which is induced by 99.9% pure acetone. By squad cotton and put it on shaved skin, after 3 days of acetone application the eczema group were divided randomly into two equal groups each group 16 rats, treat these 32 rats with glycerin and lanolin, after 24h five rats from each group were sacrificed, and remaining rats were treated with glycerin and lanolin for 72h and then sacrificed at the third day, the histopathology there is a lot of histopathological changes occur in tissue section in eczematous skin in comparison with control section; there were obvious thickness and enlargement in the length of outermost layer of skin, also there is fissures and cracks in the epidermal layer due to the dryness of skin and aggregation of inflammatory cells. And as well as keratinization of epithelial layer and changes in skin color, the results indicate there were optimum healing effect of lanolin group in compare with glycerin group after 72h, Showed back the outer most layer of the skin to its normal length, also decrease in inflammatory cells number.

scholarly journals Triazophos induced neuro-splenic toxicity and evaluation of antioxidative potential of aqueous Broccoli extract in Wistar albino rats

2021 ◽  
Vol 13 (2) ◽  
pp. 616-626
Author(s):  
Dharmender Sharma ◽  
Gurinder Kaur Sangha
Keyword(s):  
Histological Study ◽  
Control Group ◽  
Albino Rats ◽  
White Pulp ◽  
Protective Effects ◽  
Glutathione S Transferase ◽  
Group 4 ◽  
Group 5 ◽  
Group 2 ◽  
Wistar Albino Rats

The present investigation was carried out to assess the antioxidative potential of Broccoli sprouts aqueous extract (BE) against triazophos (TZ) induced oxidative stress (OS) in brain and spleen. In the experimental setup, six groups of rats were formed; Control (group 1), BE (group 2), TZ (group 3), and also BE+TZ groups such as BE1 (group 4), BE2 (group 5) and BE3 (group 6) groups. Rats were orally intubated for 30 days as per experimental design. After sacrifice, OS biomarkers viz; catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and lipid peroxidation (LPO) levels were determined in brain and spleen. Acetylcholinesterase (AChE) activity was observed in plasma and brain samples. Histological study of the spleen in TZ rats showed increased thickness of capsule, congestion and hypocellularity in follicles of spleen’s white pulp and the histoarchitecture was restored in TZ+BE group rats. TZ caused degenerative changes in brain histology and rats showed mild congestion along with haemorrhage in the cerebral cortex. Results suggest that TZ exposure is associated with neural toxicity along with altered spleen stress biomarkers, which further corroborates with histopathological findings. It is inferred that BE exerts multi-mechanistic protective effects against TZ induced neuro-splenic toxicity which is attributable to its protective antioxidant actions.

scholarly journals THE PROTECTIVE EFFECT OF THE CARTHMUS TINCTORIUS LEAVES EXTRACTON METHOTREXATE-INDUCED PULMONARY FIBROSIS IN RATS

2019 ◽  
pp. 89-92
Author(s):  
HANAA SALMAN KHADHEM ◽  
MAHDI MTHUWAINI ◽  
WAJDY J. MAJID
Keyword(s):  
Pulmonary Fibrosis ◽  
Histopathological Examination ◽  
Relative Weight ◽  
Carthamus Tinctorius ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Lung Weight ◽  
Group 2

Objective: Pulmonary fibrosis (PF) is an irreversible and untreatable human disease encompasses a large group of chronic lung disorders associated with excessive remodeling, scarring, and fibrosis. The current work was designed to study the harmful effects of methotrexate (MTX) administration on the lung and the possible protective role of Carthmus tinctorius leaves extract. The animals were utilized in this study. Methods: A total of 40 male healthy adult Wistar albino rats with anaverage body weight of 200±25 g, were divided into four groups (10 animals each). G1: control group, G2: MTX group, G3: Carthamus tinctorius (CT), group G4:MTX+Carthamus tinctorius(CT). CT was administered orally at a dose of (40 mg/kg/day) for 4 w to G3 and G4. The (CT) group were performed to explore any toxic effect of the (CT) extract on the lung. Rats of G2 and G4 administered 4 mg/kg dose of MTX orally for 28 d. Rats of G1 were intraperitoneally (i. p) administered with normal saline 0.5 ml ∕ day for four weeks (4wk) to serveas control. The animals were weighed at the beginning, though, and at the end of experiments. Results: The study showed that the relative lung weight was significantly increased at (P˂0.01) in MTX-treated animals in comparison to the control group. A combination of CT extract with MTX revealed significant decrease (P<0.01) in the lung relative weight in comparison to MTX group. Histopathological examination revealed that lung injury was less severe in group 3 and 4compared to group 2. The results indicated that CT significantly decreased collagen deposition, hydroxyproline content, and ameliorated pathological changes. Conclusion: The study has clearly identified the importance protective role of CT extract on pulmonary fibrosis induced by methoxerate. We recommended CT as one of therapeutic strategy to amelioratethe lung fibrosis associated with methotrexate therapy.

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