Antimicrobial Activity of Acanthus ebracteatus Vahl. Aqueous Extract: The Potential for Skin Infection Treatment

2009 ◽  
Vol 3 (2) ◽  
pp. 95-98 ◽  
Author(s):  
C. Sittiwet ◽  
N. Niamsa ◽  
D. Puangpronp
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Extract ◽  
Skin Infection ◽  
Infection Treatment ◽  
Acanthus Ebracteatus

Green synthesis and antimicrobial activity of ZnO nanostructures Punica granatum shell extract

2017 ◽  
Vol 6 (3) ◽  
Author(s):  
Ercan Karaköse ◽  
Hakan Çolak ◽  
Fatih Duman
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Green Synthesis ◽  
Aqueous Extract ◽  
New Technologies ◽  
Punica Granatum ◽  
Zno Nanostructures ◽  
Zno Nps ◽  
Potential Applications ◽  
Environmentally Safe

AbstractThe manufacture of nanoparticles (NPs) is a new area of investigation due to potential applications related to the improvement of new technologies; in particular, environmentally safe manufactured nanomaterials have become a growing area within nanoscience. In this research, we synthesized zinc oxide (ZnO)-NPs using an aqueous extract of

scholarly journals EVALUATION OF IN VITRO ANTI-INFLAMMATORY AND ANTIMICROBIAL ACTIVITY OF AQUEOUS AND METHANOLIC SEED EXTRACTS OF CITRULLUS LANATUS

2017 ◽  
Vol 9 (5) ◽  
pp. 29
Author(s):  
Iswariya S. ◽  
Uma T. S.
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Aqueous Extract ◽  
Seed Extract ◽  
Inflammatory Activity ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Anti Inflammatory ◽  
Seed Extracts

Objective: The present study was designed to identify the bioactive phytochemicals and its antibacterial and in vitro anti-inflammatory potential of aqueous and methanolic seed extract of Citrullus lanatus.Methods: The phytochemical screening of both the aqueous and methanolic seed extract was carried out qualitatively to identify the major Phyto-constituents present in the extracts. The antimicrobial activity of the extracts was evaluated against six pathogenic bacterial strains by agar well diffusion method and the Minimum inhibitory concentration (MIC) was determined by broth dilution method. In vitro anti-inflammatory activity of C. lanatus seed extracts was evaluated by using human red blood cell (HRBC) membrane stabilization and inhibition of albumin denaturation method.Results: The results of the study indicated that both the extracts of the seed having antimicrobial activity, while the methanolic extract showed more significant activity against the tested organism than aqueous extract. Methanol extract had the lowest MIC of 1.562 mg/ml against Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa and Bacillus subtilis, whereas in aqueous extract was highly sensitive to Bacillus subtilis, E. coli and Klebsiella pneumonia with MIC of 3.125 and 6.25 mg/ml, respectively. Methanolic extracts exerted comparative higher anti-inflammatory activity than aqueous extract.Conclusion: Present study provides a firm evidence to support that the synergistic effect of C. lanatus seed extracts having potent anti-inflammatory and antimicrobial property, which might serve as an effective drug for various microbial infections and inflammatory disorders.

scholarly journals Evaluation du potentiel antimicrobien et de la toxicité des extraits de Jatropha multifida Linn, (Euphorbiaceae)

2020 ◽  
Vol 151 ◽  
pp. 15550-15558
Author(s):  
Amégninou Agban ◽  
Yao Hoekou ◽  
Passimna Pissang ◽  
Tchadjobo Tchacondo ◽  
Komlan Batawila
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Aqueous Extract ◽  
E Coli ◽  
Toxicological Studies ◽  
Jatropha Multifida

Objectif : L’objectif de ce travail était d’évaluer in vitro l’activité antimicrobienne des extraits de feuilles et tige de Jatropha multifida sur la croissance de Candida albicans, Escherichia coli et Staphylococcus aureus, puis d’évaluer in vivo la toxicité de cette plante. Méthodologie et résultats : Les méthodes de diffusion en milieu gélosé et de microdilution en milieu liquide ont été utilisées pour évaluer l’effet antimicrobien. Une étude en subaigüe était réalisée afin d’explorer les effets toxiques de l’extrait aqueux des feuilles. Les résultats des tests antimicrobiens montrent une activité des extraits de feuilles et tige de J. multifida sur la croissance des souches utilisées avec des diamètres de zones d’inhibition allant de 8 à 25 mm et des concentrations minimales inhibitrices (CMI) variant de 0,039 mg/mL à 1,25 mg/mL à l’exception des souches de E. coli qui sont résistantes aux extraits de la tige. L’administration en subaigüe de l’extrait aqueux des feuilles de J. multifida à la dose de 600 mg/kg entraîne une perte significative de poids chez les souris. Conclusion et applications des résultats : Les extraits aqueux, éthanolique et hydroéthanolique des feuilles et tige de J. multifida possèdent d’activité antimicrobienne et pourraient être utilisés dans le traitement des Candidoses à C. albicans et des infections à S. aureus. Mais l’essai de toxicité subaigüe montre que l’extrait aqueux de la plante serait toxique. Des études toxicologiques approfondies restent donc nécessaires sur ces extraits afin de mieux élucider leur inocuité. Mots-clés : Jatropha multifida, extraits de feuilles et de tige, activités antifongique et antibactérienne, toxicité. Agban et al., J. Appl. Biosci. 2020 Evaluation du potentiel antimicrobien et de la toxicité des extraits de Jatropha multifida Linn, (Euphorbiaceae) 15551 Evaluation of antimicrobial potential and toxicity of Jatropha multifida Linn, (Euphorbiaceae) extracts ABSTRACT Objective: The objective of this study was to evaluate in vitro the antimicrobial activity of leaves and stem of Jatropha multifida extracts against Candida albicans, Escherichia coli and Staphylococcus aureus, and then to evaluate in vivo the toxicity of this plant. Methodology and Results: The agar well-diffusion and the NCCLS broth microdilution methods were used to assess the antimicrobial effect. A subacute study was carried out to explore the toxic effects of the aqueous extract of the leaves. The results of the antimicrobial tests show an activity of the extracts of leaves and stems of J. multifida on the growth of the strains used with diameters of inhibitory zones ranging from 8 to 25 mm and minimum inhibitory concentrations (MIC) varying from 0.039 mg/mL to 1.25 mg/mL exception E. coli strains which are resistant to extracts from the stem. Subacute administration of the aqueous extract of the leaves of J. multifida at a dose of 600 mg/kg leads to a significant loss of weight in the mice. Conclusion and application of findings : The aqueous, ethanolic and hydroethanolic extracts of the leaves and stem of J. multifida have antimicrobial activity and could be used in the treatment of Candidiasis and bacterial infections due respectively to C. albicans and S. aureus. But the subacute toxicity test shows that the aqueous extract of the plant would be toxic. Extensive toxicological studies therefore remain necessary on these extracts in order to better elucidate their safety. Keywords: Jatropha multifida extracts of leaves and stem, antifungal and antibacterial activities, toxicity

In vitro evaluation of antimicrobial activity and measuring its antigenic specific immune response in mice model studies: Allium cepa

2021 ◽  
pp. 2563-2568
Author(s):  
Amit Gupta ◽  
Karishma Ghosh ◽  
Dakshita Snud Sharma ◽  
Shubham Tyagi
Keyword(s):  
Cell Proliferation ◽  
Antimicrobial Activity ◽  
T Cell ◽  
Allium Cepa ◽  
Aqueous Extract ◽  
Hemolytic Activity ◽  
Cell Culture Supernatant ◽  
T Cell Proliferation ◽  
Mice Model ◽  
Model Studies

Allium cepa (onion; Liliaceae family), herbaceous annual plant and reported Sulphur based amino acids with many minerals including vitamins. Inspite of various medicinal uses of this plant as food and also showed various beneficial effects. The antimicrobial activity of aqueous extract from Allium cepa (onion bulb) was evaluated against bacterial strains using disc diffusion method. In contrast, inflammatory response or inhibition of T cell antigenic response was evaluated using typhoid vaccine and swine flu vaccine in Swiss mice. These studies were conducted according to ethical guidelines and determining T cell proliferation including estimation of cytokines from cell culture supernatant and also measuring its hemolytic activity. The results showed that aqueous extract show the highest inhibition rate against Pseudomonas aeruginosa and Salmonella enteritidis. In mice model studies, aqueous extract at higher doses showed inhibition in antigen specific T cell proliferation and also declining in the level of IFN-gamma production but enhancement in IL-4 production. In addition, aqueous extract at higher concentration does not showed any hemolytic activity. Overall, this study provides evidence for the presence of secondary metabolites in aqueous extract that probably interferes with bacterial growth (antimicrobial) and also declining in antigenic specific proliferation (i.e. anti-inflammatory) which would prevent and inhibit initiation and progression of many diseases.

scholarly journals Phytochemical Screening and Antimicrobial Activity Of Asparagus Racemosus Willd. and Asparagus Curillus Buch.-Ham. Ex Roxb

2018 ◽  
Vol 29 ◽  
pp. 91-102
Author(s):  
Rose Shrestha ◽  
Astha Shakya ◽  
Krishna Kumar Shrestha
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Extract ◽  
Ethanolic Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Asparagus Racemosus ◽  
Alcoholic Extract ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Different Strains

Asparagus racemosus Willd. is an important medicinal plant of tropical and subtropical regions of Nepal and India. Its medicinal usage has been reported in the Indian and British Pharmacopoeias and in traditional systems of medicine such as Ayurveda, Unani, and Siddha. Asparagus curillus Buch-Ham.ex Roxb. is also one of the species found in higher altitude of Nepal. Its roots are used as substitute for A. racemosus. Phytochemical investigation was done for these two species of Nepalese Asparagus as per Methodology for Analysis of Vegetable Drugs by I. Ciulei.1982. Phytochemical screening revealed the presence of coumarin, flavonoid, catecholic tannin, reducing compound in alcoholic extract of A. racemosus while its aqueous extract revealed polyuronoid, reducing compound, polyoses, saponin, gallic tannin, catecholic tannin, etc. Similarly, alcoholic extract of A. curillus revealed catecholic tannin, reducing compound and aqueous extract revealed polyuronoid polyoses, saponin, gallic tannin as main phytochemical compounds. Comparative antimicrobial activity of ethanolic extract of these two species has been evaluated using Kirby-Bauer Agar well diffusion method. The extracts were screened for their antimicrobial activity on nine different strains of human pathogenic microorganisms such as Escherichia coli, Salmonella typhi, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, Enterococcus faecalies, Saccharomyces cerevisiae and Candida albicans. Among them A. racemosus has shown selected antimicrobial effects against B. subtilis, E. coli, E. faecalis, S. cerevisiae and C. albicans with zone of inhibition of 25 mm in an average. While A. curillus showed effects on S. cerevisiae and C. albicans only with zone of inhibition about12 mm.J. Nat. Hist. Mus. Vol. 29, 2015, Page: 91-102 

scholarly journals Characterization of bacterial isolates from skin lesions of sheep, goat and cattle in different rearing condition

2019 ◽  
Vol 5 (2) ◽  
pp. 117-125 ◽  
Author(s):  
Tapu Kumar Saha ◽  
Ferdousi Begum ◽  
SM Lutful Kabir ◽  
Md Shafiqul Islam ◽  
Md Shahidur Rahman Khan
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Skin Lesion ◽  
Skin Infection ◽  
Nutrient Agar ◽  
Farm Animals ◽  
Housing System ◽  
Infection Treatment ◽  
Rearing Condition

The study was performed to isolate and characterize some selected bacteria from skin lesion of sheep, goat and cattle of different rearing condition. Intensive and semi-intensive housing systems were considered for this study. A total of (n=60) skin wound swab and pus samples were collected for this study during the study period. Among them (n=30) samples were collected from intensive farms and (n=30) samples were collected from semi-intensive farms. Staphylococcus aureus infections were found 70% skin lesion for sheep, 30% for goat and 60% for cattle and Pseudomonas aeruginosa infections were found 5% skin lesion for sheep, 15% for goat and 15% for cattle. Semi-intensive farm animals were more infected by bacteria than intensive farm animals. Staphylococcus aureus affected 53.33% in intensive farm animal and 66.67% in semi-intensive farm animal. Pseudomonas aeruginosa affected 10% animal in intensive housing system and 13.33% in semi-intensive housing system. The organisms were isolated and characterized by morphological, cultural and biochemical characteristics and identified by polymerase chain reaction. Staphylococcus aureus produced yellowish colony in nutrient agar and golden yellow colony in mannitol salt agar. Positive isolates were produced β–hemolysis in blood agar. Pseudomonas aeruginosa produced characteristic colony in nutrient agar but not produce pigment. Pseudomonas aeruginosa produced colorless colony in MaCconkey agar, violet colony in EMB agar and produced hemolysis in blood agar. Staphylococcus aureus showed Gram positive, cocci shaped and grapes like arrangement and Pseudomonas aeruginosa showed Gram negative, short rod shaped. Staphylococcus aureus fermented five basic sugars and produced gas and Pseudomonas aeruginosa fermented dextrose and mannitol. Both organisms were catalase positive, only Pseudomonas aeruginosa was oxidase positive. Pseudomonas aeruginosa isolates were further characterized by PCR amplification of toxA gene primer. Staphylococcus aureus skin infection treatment was noticed more effective by erythromycin and gentamicin and Pseudomonas aeruginosa skin infection treatment more effective by ciprofloxacin and gentamicin. Both the organisms were found to be amoxicillin resistant. This is the first and preliminary report of molecular characterization of Pseudomonas aeruginosa from skin lesion of sheep, goat and cattle associated with a different rearing condition of the animals in Bangladesh. Asian J. Med. Biol. Res. June 2019, 5(2): 117-125

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