Potentiating Effect of Piperine on Hepatoprotective Activity of Boerhaavia diffusa to Combat Oxidative Stress

Acetaminophen (APAP) overdose could induce liver damage and lead to acute liver failure. The treatment of APAP overdoses could be improved by new therapeutic strategies. Thymus spp., which has many beneficial effects and has been used in folk medicine, is one such potential strategy. In the present study, the hepatoprotective activity of the main constituents of Thymus spp., carvacrol and thymol, were evaluated in light of APAP-induced hepatotoxicity. We hoped to understand the hepatoprotective mechanism of these agents on the antioxidant system and pro-inflammatory cytokines in vitro. Dose-dependent effects of thymol and carvacrol (25, 50, and 100 µM) were tested on cultured HepG2 cells. N-Acetylcysteine (NAC) was tested as positive control. We showed that APAP inhibited HepG2 cell growth by inducing inflammation and oxidative stress. Incubating APAP-exposed HepG2 cells with carvacrol and thymol for 24 h ameliorated this inflammation and oxidative stress. We also evaluated alanine transaminase and lactate dehydrogenase levels of HepG2 cells. We found that thymol and carvacrol protected against APAP-induced toxicity in HepG2 cells by increasing antioxidant activity and reducing pro-inflammatory cytokines, such as tumor necrosis factor α and interleukin 1β. Taking together high-dose thymol and carvacrol treatment has an effect close to NAC treatment in APAP toxicity, but thymol has better treatment effect than carvacrol.

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Eugenol attenuates concanavalin A-induced hepatitis through modulation of cytokine levels and inhibition of mitochondrial oxidative stress

Archives of Biological Sciences ◽

10.2298/abs190121016l ◽

2019 ◽

Vol 71 (2) ◽

pp. 339-346

Author(s):

Jing Liu ◽

Yidong Mao

Keyword(s):

Oxidative Stress ◽

Concanavalin A ◽

Hepatoprotective Activity ◽

Antioxidant Enzyme Activities ◽

Mitochondrial Oxidative Stress ◽

Immune Mediated ◽

Wide Range ◽

Cytokine Levels ◽

Glucose 6 Phosphate Dehydrogenase

Therapeutic management of hepatitis with conventional drugs alone worsens hepatic functioning in the long term because of sustained oxidative stress. Active compounds from several plant sources have been investigated to counteract this. Eugenol, a phytochemical abundant in various plants, is known for its wide range of pharmacological effects. There is a lacuna in the deeper understanding of its hepatoprotective activity at the molecular level. Our present study aimed to determine the effects of eugenol on the changes in antioxidant components, inflammatory cytokines and modulation of mitochondrial oxidative stress in immune-mediated hepatitis. We employed a model that mimics viral hepatitis using concanavalin A (ConA) to induce T-cell-mediated acute hepatitis. Eugenol increased (P<0.01) antioxidant enzyme activities, including reduced glutathione (GSH)-regenerating enzyme, glutathione reductase, and glucose-6-phosphate dehydrogenase. Its antiinflammatory and antifibrogenic effects were evident from the reduction (P<0.01) in interleukin and tumor necrosis factor levels. Eugenol was found to decrease mitochondrial oxidative stress, which was elevated in hepatitis. The hepatoprotective effects of eugenol were confirmed by histological findings. The current investigation shows that eugenol exerts a hepatoprotective effect through the modulation of different pathways which include restoration of mitochondrial oxidative stress. Eugenol could be a promising candidate for human hepatitis management, warranting preclinical studies.

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IMPROVING EFFECT OF PLATYCODIN D ON ETHANOL-INDUCED FATTY LIVER VIA KEAP1-NRF2-ARE SIGNAL PATH

African Journal of Traditional Complementary and Alternative Medicines ◽

10.21010/ajtcam.vi15.1.15 ◽

2017 ◽

Vol 15 (1) ◽

pp. 151

Author(s):

Jingtao Wu ◽

Lei Zhou ◽

Guiwen Yang ◽

Fan Tao ◽

Maoshan Wang ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Liver Function ◽

Fatty Liver ◽

Hepatoprotective Activity ◽

Pharmacological Activities ◽

Platycodin D ◽

Signal Path ◽

Set Up ◽

Effect Of Pd

Background: Platycodin D (PD) is one of the important active ingredients in the root of the Platycodon grandiflorum (Jacq.) A. DC (PG), a traditional medicine with many pharmacological activities, especially hepatoprotective activity. There are some researches on the mechanism of liver protection, but it is not very clear. It is necessary to investigate it deeply. Materials and Methods: In order to evaluate the effect of PD on ethanol-induced fatty liver (EFL) and investigate the possible mechanism, the EFL models of rats and BRL-3A cells were set up and treated with PD of 20, 30 mg/kg body weight/day for four weeks, respectively. The hepatic histochemistry, liver function enzymes, oxidative stress levels, antioxidant enzymes and signal path enzymes were assayed. Results: PD significantly (P

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Pharmacoprophylaxis of liver diseases: creating a new hepatoprotector

BIO Web of Conferences ◽

10.1051/bioconf/20201700061 ◽

2020 ◽

Vol 17 ◽

pp. 00061

Author(s):

Svetlana Zykova ◽

Sergey Shurov ◽

Aleksey Savinkov ◽

Nino Gugushvili ◽

Vladimir Talismanov

Keyword(s):

Oxidative Stress ◽

Carbon Tetrachloride ◽

Toxic Effect ◽

Alanine Aminotransferase ◽

Liver Diseases ◽

Hepatoprotective Activity ◽

Control Group ◽

Biochemical Indicators ◽

Intact Group ◽

Biochemical Studies

The article presents a study of the hepatoprotective activity of a tricyclic heterocycle, which refers to 5, 6, 7, 8-tetrahydroquinolines. The effect of 8, 8-dimethyl-5-p-tolyl-8, 9-dihydro-2H-pyrido [4, 3, 2-de] cinnolin-3 (7H) was studied on rats under the influence of the model of toxic hepatosis induced by carbon tetrachloride to find out the indicators of peroxidation and biochemical indicators. Biochemical studies have shown that modelling toxic fat hepatosis caused by the inception of carbon tetrachloride to rats increased the activity of alanine aminotransferase by 2.5 times more compared with the intact group, indicating the development of oxidative stress induced by the treatment of pyrido [4, 3, 2] Cinnol I that reduced the toxic effect of CTC by 79.9 %. Mexidol had a less pronounced hepatoprotective effect: the activity of Alanine aminotransferase on animals of the second group was lower by 29.2 % than on rats from the control group. Thus, a new compound with hepatoprotective activity has been developed and studied.

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A Search for Hepatoprotective Activity of Fruit Extract of Mangifera indica L. Against Oxidative Stress Cytotoxicity

Plant Foods for Human Nutrition ◽

10.1007/s11130-010-0161-9 ◽

2010 ◽

Vol 65 (1) ◽

pp. 83-89 ◽

Cited By ~ 22

Author(s):

Jalal Pourahmad ◽

Mohammad Reza Eskandari ◽

Rashin Shakibaei ◽

Mohammad Kamalinejad

Keyword(s):

Oxidative Stress ◽

Mangifera Indica ◽

Hepatoprotective Activity ◽

Fruit Extract

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Hepatoprotective Triterpenes from Hairy Root Cultures of Ocimum basilicum L.

Zeitschrift für Naturforschung C ◽

10.1515/znc-2009-3-409 ◽

2009 ◽

Vol 64 (3-4) ◽

pp. 201-209 ◽

Cited By ~ 20

Author(s):

Amani M. Marzouk

Keyword(s):

Oxidative Stress ◽

Hairy Root ◽

Liver Homogenate ◽

Hepatoprotective Activity ◽

Ocimum Basilicum ◽

Albino Rats ◽

Hairy Root Cultures ◽

Root Cultures ◽

Oxidative Stress Status

Six triterpene acids identified as betulinic, oleanolic, ursolic, 3-epimaslinic, alphitolic and euscaphic acids have been isolated from a dichloromethane extract of hairy root cultures of Ocimum basilicum L. (Lamiaceae). These cultures were obtained by genetic transformation using Agrobacterium rhizogenes. The extract as well as the isolated compounds were evaluated for their hepatoprotective activity by measuring their effect on the oxidative stress status of liver, induced by carbon tetrachloride, in albino rats and in liver homogenate in vitro. All tested compounds displayed hepatoprotective activity comparable to oleanolic and ursolic acids.

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Supplementation ofCitrus maximaPeel Powder Prevented Oxidative Stress, Fibrosis, and Hepatic Damage in Carbon Tetrachloride (CCl4) Treated Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/598179 ◽

2015 ◽

Vol 2015 ◽

pp. 1-10 ◽

Cited By ~ 12

Author(s):

Mohammed Riaz Hasan Chowdhury ◽

Md Abu Taher Sagor ◽

Nabila Tabassum ◽

Md Abdullah Potol ◽

Hemayet Hossain ◽

...

Keyword(s):

Oxidative Stress ◽

Phenolic Compounds ◽

Carbon Tetrachloride ◽

Inflammatory Cells ◽

Hepatoprotective Activity ◽

Oxidation Products ◽

Iron Deposition ◽

Stress Markers ◽

Citrus Maxima ◽

Natural Phenolic Compounds

Citrus maximapeel is rich in natural phenolic compounds and has a long use in the traditional medicine. HPLC-DAD analysis onCitrus maximapeel powder exhibited the presence of various phenolic compounds such as caffeic acid and (−)-epicatechin. To determine the plausible hepatoprotective activity ofCitrus maximapeel powder, we used carbon tetrachloride (CCl4) treated rat model. Liver damage in rats was confirmed by measuring the AST, ALT, and ALP enzyme activities. In addition, lipid peroxidation products (MDA), nitric oxide, advanced protein oxidation products level (APOP), and catalase activities were also analyzed along with the histological profiling for the inflammatory cell infiltration, collagen, and iron deposition in liver. Dietary supplementation ofCitrus maximapeel powder exhibited significant reduction of serum AST, ALT, and ALP activities in carbon tetrachloride treated rats. Moreover,Citrus maximapeel powder also showed a significant reduction of the oxidative stress markers (MDA, NO, and APOP level) and restored the catalase activity in CCl4treated rats. Histological examination of the liver section revealed reduced inflammatory cells infiltration, collagen, and iron deposition in CCl4treated rats. The results from this study demonstrated thatCitrus maximapeel powder produced significant hepatoprotective action in CCl4administered rats.

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In Vitroandin VivoAntioxidant Activity of Alfalfa (Medicago sativaL.) on Carbon Tetrachloride Intoxicated Rats

The American Journal of Chinese Medicine ◽

10.1142/s0192415x12500589 ◽

2012 ◽

Vol 40 (04) ◽

pp. 779-793 ◽

Cited By ~ 9

Author(s):

Mohammed S. Al-Dosari

Keyword(s):

Oxidative Stress ◽

Carbon Tetrachloride ◽

Lipid Profile ◽

Histopathological Examination ◽

Serum Levels ◽

Hepatoprotective Activity ◽

Test Substance ◽

Protein Sulfhydryl ◽

Serum Transaminases

The present study was conducted to determine whether lyophilized aqueous extract of alfalfa, or Medicago sativa L. could exert antioxidant activity against carbon tetrachloride-induced oxidative stress and liver injury in rats. The hepatoprotective activity of alfalfa extract was determined by assessing the levels of serum transaminases, ALP, bilirubin and lipid profile. Further, the effect of the test substance on malondialdehyde (MDA), an end product of lipid peroxidation; antioxidant liver enzyme non-protein sulfhydryl (NP-SH); and total protein (TP) were also studied. Serum transaminase, ALP, bilirubin level, lipid profile and liver MDA were significantly elevated and the antioxidant status in liver NP-SH and TP contents were declined in animals treated with CCl4alone. Pretreatment with alfalfa and silymarin for three weeks prior to the administration of CCl4significantly prevented the increase in the serum levels of hepatic marker, LDL, VLDL levels enzymes and reduced oxidative stress indicated by elevated NP-SH and TP concentration. The histopathological examination of the livers also showed that the alfalfa extract reduced the incidence of liver lesions induced by CCl4. The in vitro antioxidant assessment of alfalfa extract on DPPH and carotene-linoleic assays demonstrated a moderate antioxidant potential. Results suggest that the alfalfa extract possesses hepatoprotective and antioxidative stress properties possibly through its antioxidant phytochemical constituents and substantiates its use in various liver disorders as a hepatoprotector.

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EVALUATION OF “LEUCAS LANATA” AND ASSESSMENT OF ITS HEPATOPROTECTIVE EFFECTS ON ANTITUBERCULAR DRUG-INDUCED HEPATIC DAMAGE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i6.25888 ◽

2018 ◽

Vol 11 (6) ◽

pp. 451

Author(s):

Pritt Verma ◽

Sajal Srivastava ◽

Ch. V. Rao

Keyword(s):

Oxidative Stress ◽

Interleukin 1 ◽

Hepatoprotective Activity ◽

Hepatic Damage ◽

Control Group ◽

Antitubercular Drug ◽

Oxidative Stress Parameter ◽

Traditional Use ◽

Toxic Dose ◽

Group I

Objective: The objective of this study was to investigate the hepatoprotective activity of ethanolic whole plant extract of Leucas lanata (family - Lamiaceae) against rifampicin+isoniazid (RIF+INH) induced hepatic damage in rats.Methods: Wistar rats of either sex were divided into five groups of six animals each and given orally the following treatment for 28 days. The normal control was given 1% CMC 1 ml/kg body weight (b.w.), RIF+INH antitubercular drug (ATT) at a dose of 50 mg/kg b.w., p.o. was given a toxic dose for inducing hepatotoxicity. Silymarin (100 mg/kg, p.o) was given as reference standard. Two different doses of Lucas lanata extract of 200 and 400 mg/kg, p.o were tested for hepatoprotective activity. At the end of the treatment, blood was collected from direct cardiac puncture and analyzed for numerous bodily fluid parameter such as alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphate (ALP), total protein (TP), and total bilirubin (TB) and oxidative stress parameter lipid peroxidation (LPO), superoxide dismutase (SOD), catalase, glutathione (GSH), malondialdehyde and also evaluated the hydroxyproline content, tumor necrosis factor alpha, and interleukin-1 beta in all groups. Livers were isolated for its essential estimation followed by histopathological studies.Results: Hepatic serum markers (ALT, AST, ALP, albumins, TP, and TB) were significant (p<0.01–p<0.001) protective effect was obtained against ATT-induced liver damage, and ATT treatment significantly decreased LPO, SOD, and GSH (p<0.001) levels compared with control Group I and given a Leucas lanata extract (LLE) (p<0.01–p<0.001), the oxidative stress markers were markedly reversed. Histopathology of liver tissue showed that LLE attenuated the hepatocellular necrosis, regeneration, and repair of cells toward normal. LLE which was standardized using high-performance thin-layer chromatography revealed the presence of some critical phenolic (gallic acid and quercetin) compound.Conclusion: The results of this study powerfully indicate the protecting result of LLE against liver injury which can be attributed to its hepatoprotective activity, and thereby scientifically support its traditional use.

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