Phytochemical Screening and Antioxidant Activity of Ethanolic Extract of Rhizanthes deceptor (Rafflesiaceae) and its Host Tetrastigma papillosum

2015 ◽  
Vol 9 (6) ◽  
pp. 293-299
Author(s):  
Z. Saleh ◽  
E.A.M. Zuhud ◽  
R.K. Sari
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Phytochemical Screening

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

scholarly journals Phytochemical, physicochemical, TLC, minerals analysis and in-vitro antioxidant activity of ethanolic extract of leaves of Heldigardia populifolia

2018 ◽  
Vol 1 (1) ◽  
pp. 22-26
Author(s):  
T Bhanumathi ◽  
P Keerthana ◽  
A Cheenakesavulu ◽  
M Neeharika ◽  
E Sandhya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Permissible Limits ◽  
Leaf Powder ◽  
Composition Of Minerals ◽  
Tlc Analysis ◽  
In Vitro Antioxidant Activity

The aim of present study was to investigate the preliminary phytochemical, physicochemical, TLC, minerals analysis and In-vitro antioxidant activity of leaves of ethanolic extract of Heldigardia populifolia. The preliminary phytochemical screening of ethanolic extract showed the presence of triterpenoids, flavonoids, glycosides, sterols, steroids, phenols, carbohydrates and saponins. The composition of minerals found in the leaf powder was within the permissible limits. TLC analysis of ethanol extract showed the five spots which indicate the presence of five phytoconstituents. The extractive value of ethanol was high than acetone. Ash values were within the limits. The in-vitro antioxidant activity of ethanolic extract increased with increasing the concentration. The ethanolic extract in all the concentration showed the significant antioxidant activity.

scholarly journals The The Phytochemical Screening, Total Phenolic Contents and Antioxidant Activities in Vitro of White Oyster Mushroom (Pleurotus Ostreatus) Preparations

2019 ◽  
Vol 7 (15) ◽  
pp. 2404-2412
Author(s):  
Santun Bhekti Rahimah ◽  
Dhiah Dianawaty Djunaedi ◽  
Arto Yuwono Soeroto ◽  
Tatang Bisri
Keyword(s):  
Antioxidant Activity ◽  
Pleurotus Ostreatus ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Ethanolic Extracts ◽  
Active Substances

BACKGROUND: The popular commercially cultivated Pleurotus ostreatus mushroom contains very high nutrients and bioactive compounds with high antioxidant activity. The ethanolic extract seems to be the most active in preparation. AIM: This study has an aim to compare the phytochemical analysis of a fresh, dry and ethanolic extract of Pleurotus ostreatus, to measure the total phenolic content and antioxidant activities in vitro of ethanolic extracts of Pleurotus ostreatus. METHODS: The fresh plant's materials (FPM), dry plants materials (DPM), ethanolic extracts were macerated with 70% (EE70) and 96% ethanol (EE96) of Pleurotus ostreatus which were used for phytochemical analysis, and EE96 was used for antioxidant activity in vitro. The phytochemical analysis was conducted using the Dragendorf and Meyer, FeCl3 test, Salkowsky method, Lieberman method, amyl alcohol, foam test and the NaOH reagent. The total phenol test was carried out using the Follin-Ciocalteu method. The antioxidant activity was tested using the ABTS and H2O2 essay. RESULTS: The phytochemical screening showed that the flavonoid, phenolic compounds, tannin, saponin, alkaloids, and steroids were detected in the FPM, DPM, EE70 and also the EE96. The alkaloid, however, was not identified by the Meyer Reagent in the FPM and DPM. The DPM and EE70 seemed to have the highest amount of saponin based on the foam that was formed. Meanwhile, steroids and flavonoids were detected at a higher level in the EE96, based on the strength of visible colour. However, triterpenoid and quinones could not be identified. In the total phenol test, there was an amount of 6.67 μg phenol in a 1 mg extract sample which was equivalent to 1 mg of Gallic Acid. The EE96 has an IC50 of 108.07 μg/mL for ABTS and an IC50 reduction of 229.17 μg/mL. The process of Pleurotus ostreatus drying did not reduce the content of active substances. The polar active substances seem to be more soluble in the EE70 than the EE96. CONCLUSION: The higher the bioactive substances in the preparation, the more significant the bio-therapeutic effects. Ethanolic extract of Pleurotus ostreatus has a phenol content and a good antioxidant action.  

scholarly journals Phytochemical Screening and Antioxidant Activity of Ethanolic Extract of Cawat Hanoman Stem (Bauhinia aculeata L.) using DPPH Method

2020 ◽  
Vol 3 (1) ◽  
pp. 15-21
Author(s):  
Rahmi Muthia ◽  
Muhammad Hidayatullah ◽  
Rahmi Hidayati
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method ◽  
Layer Chromatography ◽  
Unstable Molecule

The free radical is an unstable molecule because contains one or two unpaired electrons. The antioxidant substance is a simple way to decrease the illness caused by free radicals. Cawat hanoman (Bauhinia aculeata L.) was known to contain tannin components one of the benefits as an antioxidant. This research aims to determine the antioxidant activity of the B. aculeata stem tested by qualitatively used thin-layer chromatography (TLC) and quantitatively using the DPPH method. Bauhinia aculeata stem was extracted using a maceration extract method with 96% ethanol. Antioxidant activity test was done qualitatively by eluent of ethyl acetate : methanol : purified water (6 : 2 : 1) using TLC and quantitatively using the DPPH method. The result of antioxidant activity from 96% ethanol extract of B. aculeata stem qualitatively showed the presence of yellow spots on a purple background at TLC after syringed DPPH 0.5 mM and quantitative test that resulted in an IC50 of 21.862 �g/mL. These results indicate that 96% ethanol extract of B. aculeata has very strong antioxidant activity.

scholarly journals PHYTOCHEMICAL SCREENING OF ETHANOLIC EXTRACT OF WHOLE PLANT OF SIDA GLUTINOSA

2020 ◽  
pp. 65-74
Author(s):  
GUDURU RAJESWARI ◽  
SWARNA LATHA D ◽  
CHANDRA SEKHAR KB
Keyword(s):  
Antioxidant Activity ◽  
High Pressure ◽  
Total Phenolics ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Isolation And Characterization ◽  
Rp Hplc ◽  
Whole Plant ◽  
Ic50 Value ◽  
Layer Chromatography

Objective: The aim of the present study is to screen for the phytochemical constituents which are of pharmacological importance present in the ethanolic extract of whole plant of Sida glutinosa (SG). Methods: The ethanolic extract of the dried whole plant of SG is subjected to preliminary phytochemical screening which showed the presence of major phytoconstituents such as phenols, flavonoids, and alkaloids. The extract was screened for its antioxidant activity by 2,2-diphenyl-1- picrylhydrazyl, hydroxyl radical, Iron (III) to Iron (II) reducing activity, and nitric oxide scavenging assay. Further, the ethanolic extract was subjected to fingerprinting technique high-pressure thin-layer chromatography (HPTLC). Reverse phase high-pressure liquid chromatography (Rp-HPLC) was performed to estimate the amount of total phenolics, flavonoids, and alkaloids quantitatively in isocratic mode. Results: Phytochemical screening of the ethanolic extract of the plant showed the presence of pharmacologically important constituents such as alkaloids, flavonoids, phenolics, and terpenoids. The study also revealed the potential antioxidant activity of the extract with IC50 value. The extract fingerprinting through HPTLC revealed the presence of various phytoconstituents. Rp-HPLC showed 0.35±0.12 μg/ml of total phenolics, 0.0013±0.05μg/ml of alkaloids, and 0.00081±0.08 μg/ml of flavonoids. Conclusion: Scientific evaluation of SG which has therapeutic significance was carried out which is an important concept for the standardization of the plant-based drug. Further, there is a need for isolation and characterization of the lead molecules their systemic evaluation for its pharmacological activities.

scholarly journals Antimicrobial and antioxidant activity of essential oil and different plant extracts of Psidium cattleianum Sabine

2016 ◽  
Vol 76 (1) ◽  
pp. 101-108 ◽  
Author(s):  
M. C. Scur ◽  
F. G. S. Pinto ◽  
J. A. Pandini ◽  
W. F. Costa ◽  
C. W. Leite ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Plant Extracts ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Psidium Cattleianum ◽  
Ethanolic Extracts

Abstract The goals of the study were to determinethe antimicrobial and antioxidant activities of essential oil and plant extracts aqueous and ethanolic of Psidium cattleianum Sabine; the chemical composition of the essential oil of P. cattleianum; and the phytochemical screening of aqueous and ethanolic extracts of the same plant. Regarding the antimicrobial activity, the ethanolic extract exhibited moderate antimicrobial activity with respect to bacteria K. pneumoniae and S. epidermidis, whereas, regarding other microorganisms, it showed activity considered weak. The aqueous extract and the essential oil showed activity considered weak, although they inhibited the growth of microorganisms. About the antioxidant potential, the ethanolic and aqueous extracts exhibited a scavenging index exceeding 90%, while the essential oil didn´t show significant antioxidant activity. Regarding the phytochemical composition, the largest class of volatile compounds identified in the essential oil of P. cattleianum included the following terpenic hydrocarbons: α-copaene (22%); eucalyptol (15%), δ-cadinene (9.63%) and α-selinene (6.5%). The phytochemical screening of extracts showed the presence of tannins, flavonoids, and triterpenoids for aqueous and ethanolic extracts. The extracts and essential oils inhibit the growth of microrganisms and plant extracts showed significant antioxidant activity. Also, the phytochemical characterization of the essential oil showed the presence of compounds interest commercial, as well as extracts showed the presence of important classes and compounds with biological activities.

scholarly journals FORMULATION OF ANTIOXIDANT GEL FROM STANDARDIZED GREEN CINCAU (CYCLEA BARBATA L. MIERS) ETHANOLIC EXTRACT

2020 ◽  
pp. 236-240
Author(s):  
ERLINDHA GANGGA ◽  
YUNAHARA FARIDA ◽  
KARTININGSIH
Keyword(s):  
Antioxidant Activity ◽  
Microbial Contamination ◽  
Ph Value ◽  
Ethanolic Extract ◽  
Premature Aging ◽  
Phytochemical Screening ◽  
Active Ingredients ◽  
Greenish Yellow ◽  
Ic50 Value ◽  
Ethanolic Extracts

Objective: The aim of this study to develop an antioxidant gel from standardized green cincau (Cyclea barbata L. Miers) ethanolic extracts. Methods: The standardized extract of green cincau (Cyclea barbata L. Miers) ethanolic extracts were formulated as active ingredients of antioxidant gel. The dried leaf of Cyclea barbata L Miers was extracted with ethanol 70%. The extract was analyzed its phytochemical screening and antioxidant activity with DPPH methods. The extract was used as active ingredients of antioxidant gel. The gels were evaluated physically and chemically, such as organoleptic test, homogeneity, viscosity and rheology, spreadability, pH test, antioxidant activity, and analysis of microbial contamination test. Results: The results showed that all formulas were greenish-yellow and have homogeneous, the viscosity of 14267-45533 cps, spreadability of gels 1783.8–6631.5 mm2, pH value of 4.57–5.74, and analysis of microbial contamination has colonies not more than 103 colonies/g or colonies/ml. Statistical analysis used two ways ANOVA to investigate gels time and temperature effects toward gel preparations stability. There was no effect on the viscosity, spreadability, and pH tests, on the all formula with P>0.05. The antioxidant activity of the formula showed that all formulas changed after 3 mo of storage at 40 °C. Formula II had stronger antioxidant activity than the others with an IC50 value of 57.60µg/ml. Conclusion: All formulas showed fairly strong antioxidant activity because it can inhibit the activity of free radicals that could inhibit the premature aging of the skin.

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