scholarly journals Antimicrobial and antioxidant activity of essential oil and different plant extracts of Psidium cattleianum Sabine

2016 ◽  
Vol 76 (1) ◽  
pp. 101-108 ◽  
Author(s):  
M. C. Scur ◽  
F. G. S. Pinto ◽  
J. A. Pandini ◽  
W. F. Costa ◽  
C. W. Leite ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Plant Extracts ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Psidium Cattleianum ◽  
Ethanolic Extracts

Abstract The goals of the study were to determinethe antimicrobial and antioxidant activities of essential oil and plant extracts aqueous and ethanolic of Psidium cattleianum Sabine; the chemical composition of the essential oil of P. cattleianum; and the phytochemical screening of aqueous and ethanolic extracts of the same plant. Regarding the antimicrobial activity, the ethanolic extract exhibited moderate antimicrobial activity with respect to bacteria K. pneumoniae and S. epidermidis, whereas, regarding other microorganisms, it showed activity considered weak. The aqueous extract and the essential oil showed activity considered weak, although they inhibited the growth of microorganisms. About the antioxidant potential, the ethanolic and aqueous extracts exhibited a scavenging index exceeding 90%, while the essential oil didn´t show significant antioxidant activity. Regarding the phytochemical composition, the largest class of volatile compounds identified in the essential oil of P. cattleianum included the following terpenic hydrocarbons: α-copaene (22%); eucalyptol (15%), δ-cadinene (9.63%) and α-selinene (6.5%). The phytochemical screening of extracts showed the presence of tannins, flavonoids, and triterpenoids for aqueous and ethanolic extracts. The extracts and essential oils inhibit the growth of microrganisms and plant extracts showed significant antioxidant activity. Also, the phytochemical characterization of the essential oil showed the presence of compounds interest commercial, as well as extracts showed the presence of important classes and compounds with biological activities.

scholarly journals The The Phytochemical Screening, Total Phenolic Contents and Antioxidant Activities in Vitro of White Oyster Mushroom (Pleurotus Ostreatus) Preparations

2019 ◽  
Vol 7 (15) ◽  
pp. 2404-2412
Author(s):  
Santun Bhekti Rahimah ◽  
Dhiah Dianawaty Djunaedi ◽  
Arto Yuwono Soeroto ◽  
Tatang Bisri
Keyword(s):  
Antioxidant Activity ◽  
Pleurotus Ostreatus ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Ethanolic Extracts ◽  
Active Substances

BACKGROUND: The popular commercially cultivated Pleurotus ostreatus mushroom contains very high nutrients and bioactive compounds with high antioxidant activity. The ethanolic extract seems to be the most active in preparation. AIM: This study has an aim to compare the phytochemical analysis of a fresh, dry and ethanolic extract of Pleurotus ostreatus, to measure the total phenolic content and antioxidant activities in vitro of ethanolic extracts of Pleurotus ostreatus. METHODS: The fresh plant's materials (FPM), dry plants materials (DPM), ethanolic extracts were macerated with 70% (EE70) and 96% ethanol (EE96) of Pleurotus ostreatus which were used for phytochemical analysis, and EE96 was used for antioxidant activity in vitro. The phytochemical analysis was conducted using the Dragendorf and Meyer, FeCl3 test, Salkowsky method, Lieberman method, amyl alcohol, foam test and the NaOH reagent. The total phenol test was carried out using the Follin-Ciocalteu method. The antioxidant activity was tested using the ABTS and H2O2 essay. RESULTS: The phytochemical screening showed that the flavonoid, phenolic compounds, tannin, saponin, alkaloids, and steroids were detected in the FPM, DPM, EE70 and also the EE96. The alkaloid, however, was not identified by the Meyer Reagent in the FPM and DPM. The DPM and EE70 seemed to have the highest amount of saponin based on the foam that was formed. Meanwhile, steroids and flavonoids were detected at a higher level in the EE96, based on the strength of visible colour. However, triterpenoid and quinones could not be identified. In the total phenol test, there was an amount of 6.67 μg phenol in a 1 mg extract sample which was equivalent to 1 mg of Gallic Acid. The EE96 has an IC50 of 108.07 μg/mL for ABTS and an IC50 reduction of 229.17 μg/mL. The process of Pleurotus ostreatus drying did not reduce the content of active substances. The polar active substances seem to be more soluble in the EE70 than the EE96. CONCLUSION: The higher the bioactive substances in the preparation, the more significant the bio-therapeutic effects. Ethanolic extract of Pleurotus ostreatus has a phenol content and a good antioxidant action.  

scholarly journals Antioxidant and Antimicrobial Activity of Mexican Oregano (Poliomintha longiflora) Essential Oil, Hydrosol and Extracts from Waste Solid Residues

Plants ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 22 ◽  
Author(s):  
Teresa Cid-Pérez ◽  
Raúl Ávila-Sosa ◽  
Carlos Ochoa-Velasco ◽  
Blanca Rivera-Chavira ◽  
Guadalupe Nevárez-Moorillón
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Chemical Components ◽  
Waste Solid ◽  
Ethyl Acetate Extracts

Poliomintha longiflora is a Mexican oregano, which has not been widely studied. This work aimed to describe the chemical composition, antimicrobial and antioxidant activities present in P. longiflora essential oil (EO), the hydrosol from EO extraction and extracts from waste solid residues (WSRs), identified as ethanol extract, ethyl acetate extract and the subfractions of ethanol and ethyl acetate extracts. The chemical characterization of the EO, hydrosol and WSR extracts was performed by GC–MS and HPLC. Their antioxidant activity was evaluated using two methods, and their antimicrobial activity was evaluated against Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, and Salmonella Typhimurium. Thirty-one chemical components were identified in the EO. The subfractions from the ethanol and ethyl acetate extracts contain methylmaleic anhydride, thymoquinone, thymol, carvacrol, thymol acetate, carvacrol acetate, and phenolic acids. The EO presented the highest biological activities for antioxidant (136.05 mg equivalent of ascorbic acid/g (AAE/g); IC50 83.70 μg/mL of 2, 2-diphenyl-1-picrylhydrazyl (DPPH)) and antimicrobial tests (minimal inhibitory concentration (MIC) value of 250–750 mg/L), while the hydrosol and the ethyl acetate extract from WSRs had the lowest antioxidant activity (14.16 and 12.29 mg AAE/g respectively), and the hydrosol had the lowest antimicrobial activity (MIC of 3000 mg/L). The data suggest that Mexican oregano P. longiflora hydrosol and extracts from waste solid residues can still have compounds with antimicrobial and antioxidant capacities.

Evaluation of antioxidant and antimicrobial activity of Ziziphora ‎clinopodiodes essential oil eluted at different times

2020 ◽  
Vol 16 ◽  
Author(s):  
Mojgan Alizadeh ◽  
Akram Arianfar ◽  
Ameneh Mohammadi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Positive Control ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Citronellic Acid ◽  
Antimicrobial And Antioxidant Activity

Objective: Ziziphora clinopodioides is an edible medicinal plant belongs to the Labiatae family that widespread all over Iran. It used as culinary and also in cold and cough treatments in Iran. The aim of present work was to evaluate the effect of different timeframes during the hydrodistillation on essential oil composition, antimicrobial and antioxidant activity. Materials and Methods: The essential oil of Z. clinopodiodes was extracted via hydrodistillation with Clevenger apparatus. The fractions of essential oil were captured at 6 times from the beginning of the distillation: (10, 20, 60, 120, 180 and 240 min). The fractions of essential oil were analyzed by GC/MS and their antibacterial, antifungal and antioxidant activities were studied by Disk - well diffusion and DPPH methods respectively. Results: Six distillation times and whole essential oil were captured during the hydrodistillation. Essential oil yield dropped off significantly during distillation progressed (1.0% for 10 min and 0.025 for 240 min). 1,8 Cineol, Isomenthone, Pulegone, Piperitenone and Citronellic acid were major compounds in fractions and they were affected by distillation times. Pulegone was major compound in all of essential oils. In antioxidant activity assay, whole essential oil was stronger than was stronger than positive control and fractions of essential oil, because of higher levels of Isomenthone, Piperitenone and Citronellic acid. Strongest antimicrobial activity against S. aureus, E. coli and C. albicans was observed from 10 min fraction. Conclusion: Our results indicated that distillation time can create essential oils with specific properties and we can achieve to more efficient essential oil in short times.

scholarly journals Thymus mastichina: Composition and Biological Properties with a Focus on Antimicrobial Activity

2020 ◽  
Vol 13 (12) ◽  
pp. 479
Author(s):  
Márcio Rodrigues ◽  
Ana Clara Lopes ◽  
Filipa Vaz ◽  
Melanie Filipe ◽  
Gilberto Alves ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Biological Activities ◽  
Scientific Information ◽  
Biological Properties ◽  
Fusarium Spp ◽  
Candida Spp ◽  
Pharmaceutical Industries

Thymus mastichina has the appearance of a semishrub and can be found in jungles and rocky lands of the Iberian Peninsula. This work aimed to review and gather available scientific information on the composition and biological properties of T. mastichina. The main constituents of T. mastichina essential oil are 1,8-cineole (or eucalyptol) and linalool, while the extracts are characterized by the presence of flavonoids, phenolic acids, and terpenes. The essential oil and extracts of T. mastichina have demonstrated a wide diversity of biological activities. They showed antibacterial activity against several bacteria such as Escherichia coli, Proteus mirabilis, Salmonella subsp., methicillin-resistant and methicillin-sensitive Staphylococcus aureus, Listeria monocytogenes EGD, Bacillus cereus, and Pseudomonas, among others, and antifungal activity against Candida spp. and Fusarium spp. Additionally, it has antioxidant activity, which has been evaluated through different methods. Furthermore, other activities have also been studied, such as anticancer, antiviral, insecticidal, repellent, anti-Alzheimer, and anti-inflammatory activity. In conclusion, considering the biological activities reported for the essential oil and extracts of T. mastichina, its potential as a preservative agent could be explored to be used in the food, cosmetic, or pharmaceutical industries.

scholarly journals Screening of phytochemical compounds, trace metals and antimicrobial activity of Anacyclus pyrethrum

2016 ◽  
Vol 2 (1) ◽  
pp. 32 ◽  
Author(s):  
G. Subasri ◽  
S. Ahmed John
Keyword(s):  
Antimicrobial Activity ◽  
Trace Metals ◽  
Ethanolic Extract ◽  
Reducing Sugar ◽  
Plant Sample ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Ethanolic Extracts ◽  
Phytochemical Compounds ◽  
Positive Results

In the present study is phytochemicals, trace metals and antimicrobial activity of ethanolic extract of Anacyclus pyrethrum. The phytochemical screening of the crude ethanolic root, stem and leaf extracts showed the positive results of steroids, triterpenes, reducing sugar, sugar, alkaloids, flavonoids, saponin, tannins, anthraquinones and amino acids. The average mean concentrations of Cd, Cr, Cu, Fe, Ni, Pb and Zn in plant sample were BDL, 0.03, 0.42, 0.79, BDL, BDL and 0.58 mg kg-1, respectively. The descending order of the metal content in the plant sample were: Zn > Fe > Cu > Cd > Cr = Ni = Pb. Among various part of plant extracts studied for antimicrobial activity, root ethanolic extract showed highest of inhibition than leaves and stem ethanolic extracts.

Phytochemical Profile, Antimicrobial and Antioxidant Activities of Cistus clusii Essential oil From Algeria

2020 ◽  
Vol 16 (2) ◽  
pp. 109-114
Author(s):  
Souad Khebri ◽  
Naima Benkiki ◽  
Mouloud Yahia ◽  
Hamada Haba
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
Diffusion Method ◽  
Β Carotene

Objective: In this work, we investigated the chemical composition and the antioxidant and antimicrobial activities of the Algerian Cistus clusii Dunal essential oil. Methods: Cistus clusii essential oil has been extracted using hydrodistillation method and characterized by GC/MS. The antioxidant activity was evaluated by two different methods DPPH free radical scavenging, and β-carotene bleaching test. The antimicrobial activity was carried out using disc diffusion method against reference strains; Pseudomonas aeruginosa (ATCC 27865), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), clinical bacterial (Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus) and fungal (Candida albicans) isolates. Results: The chemical profile of the oil showed 31 compounds. This profile was dominated by the presence of monoterpene hydrocarbons (78.4%), oxygenated derivatives (18.8%) and sesquiterpene hydrocarbons as minor constituents of this essential oil (1.9%). The major constituents were Camphene (20.6%), γ-Terpinene (16.5%), Sabinene (12.8%), and Terpineol-4 (13.5%). Other compounds were found in moderately good amounts like α-Terpinene (8.9%), α-Thujene (5.9%), α-Pinene (4.2%), and α- Terpinolene (3.1%). The results of the antimicrobial activity showed that the highest activity was observed against C. albicans and it was higher than positive control Fluconazole. The essential oil exhibited a strong antioxidant activity, especially in the β-carotene system. Conclusion: This study indicates that Cistus clusii essential oil showed potent antioxidant and moderate antimicrobial activities which could be used to explain the use of this species in traditional medicine.

scholarly journals HPLC Analysis and Antioxidant Evaluation of Acteoside-Rich Osmanthus fragrans Extracts

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Sung-Ho Lee ◽  
Seong-Wook Seo ◽  
Seung-Yub Song ◽  
Deuk-Sil Oh ◽  
Jung-Hyun Shim ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Hot Water ◽  
Total Phenolic ◽  
Osmanthus Fragrans

A method for the separation and quantification of acteoside by reverse-phase high-performance liquid chromatography (HPLC) was developed and validated. Hot water and ethanolic extracts of Osmanthus fragrans leaves and flowers were analyzed for acteoside content. Excellent linearity was obtained, with an r2 higher than 0.999. The precision, specificity, and accuracy of our method were excellent, suggesting that it can be conveniently used for the quantification of acteoside in the crude extract of O. fragrans. The hot water and ethanol extracts were analyzed, and their biological activities were tested. The extraction yields, marker (acteoside) contents, and antioxidant activities of the leaf and flower extracts were analyzed. The antioxidant activity was confirmed by measuring the 2,2-diphenyl-2-picrylhydrazyl radical scavenging activity, reducing power, and total phenolic content. The acteoside content tended to be higher in the 100% ethanol extract of O. fragrans compared to those with the other extraction conditions tested. Overall, almost all extracts prepared with ethanolic solvents tended to produce better antioxidant activity than those prepared with hot water. These results suggest that the ethanolic extract of O. fragrans could serve as a potential antioxidant and anti-inflammatory pharmaceutical source, and our validated method would be useful for the quality control of O. fragrans extracts.

scholarly journals Phytochemical screening and biological activities of pakoba (Syzygium luzonense) stem bark ethanol extract

2020 ◽  
Vol 21 (6) ◽  
Author(s):  
Mario Walean ◽  
ROSTINA MELPIN ◽  
MERVINA RONDONUWU ◽  
KINZIE FELICIANO PINONTOAN ◽  
Hendra Pratama Maliangkay ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Activity Index ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Optimum Dose ◽  
Phytochemical Analysis ◽  
Phytochemical Screening

Abstract. Walean M, Melpin R, Rondonuwu M, Pinontoan KF, Maliangkay HP, Astriani M. 2020. Phytochemical screening and biological activities of pakoba (Syzygium luzonense) stem bark ethanol extract. Biodiversitas 21: 2377-2382. Pakoba (Syzygium luzonense (Merr.) Merr) as an endemic and ethnomedical plant of North Sulawesi was investigated for its phytochemical constituents and biological activities. The presence of phytochemicals constituents, antibacterial, antioxidant activities, and antihyperglycemic of pakoba stem bark ethanolic extract (PSBEE) were also investigated. Pakoba stem bark was extracted using ethanol. The extracts were evaluated for antimicrobial activity using the Kirby Bauer method, whereas antioxidant activity was determined by using 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay. Alloxan-induced diabetic rats investigation was used to determine the antihyperglycemic activity. The phytochemical analysis revealed that tannins, alkaloids, and flavonoids were present in PSBEE. PSBEE has potent antioxidant activity with antioxidant activity index = 1.02. PSBEE significantly reduced the blood sugar of hyperglycemic rats with an optimum dose of 300 mg/kgbw. In this study, PSBEE has weak antibacterial activity against in Escherichia coli and Staphylococcus aureus.

Upcycling Culinary Organic Waste: Production of Plant Particles from Potato and Carrot Peels to Improve Antioxidative Capacity

2020 ◽  
Vol 01 ◽  
Author(s):  
Dalia A. Yassin ◽  
Muhammad Jawad Nasim ◽  
Abraham M. Abraham ◽  
Cornelia M. Keck ◽  
Claus Jacob
Keyword(s):  
Antioxidant Activity ◽  
High Speed ◽  
Antioxidant Activities ◽  
Raw Materials ◽  
Ethanolic Extract ◽  
Correlation Spectroscopy ◽  
Waste Production ◽  
Biological Potential ◽  
Ethanolic Extracts ◽  
Vegetables And Fruits

Background: Vegetables and fruits are consumed in considerable amounts worldwide producing huge quantities of organic leftovers comprising primarily of peels. Peels of potatoes (PP) and carrots (CP), for instance, are often considered as waste, albeit they still represent a rich source of interesting phytochemicals. Traditional waste management of such materials, usually vermicomposting, therefore represents a low value approach and also a considerable burden to the environment. Objective: Aiming to turn some of this waste into raw materials for further applications, methods were explored to prepare suspensions of PP and CP. Antioxidant activities of these suspensions were compared to bulk-suspensions and the corresponding ethanolic extracts in anticipation of possible applications in Nutrition and Cosmetics. Methods: The peels of potatoes and carrots were subjected to high speed stirring (HSS) and high pressure homogenization (HPH) to produce the suspensions which were characterized for size distribution by Laser Diffraction (LD), Photon Correlation Spectroscopy (PCS) and light microscopy (LM). The ethanolic extracts of peels were also produced. All of the samples were evaluated for antioxidant activity employing 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Results: HPH produced suspensions of peels comprised of particles with diameters in the range of 268 - 335 nm for PP and 654 - 1,560 nm for CP. These suspensions exhibited a significantly stronger antioxidant activity compared to the bulk-suspensions. Moreover, the suspension of PP (1% w/w) exhibited comparable antioxidant activity to the ethanolic extract (1% w/w) whilst the CP suspension (1% w/w) exhibited lower activity compared to the ethanolic extract. Conclusion: Production of suspensions of vegetable peels may unlock some biological potential which could be optimised for applications in Nutrition, Agriculture, Medicine and Cosmetics.

scholarly journals FORMULATION OF ANTIOXIDANT GEL FROM STANDARDIZED GREEN CINCAU (CYCLEA BARBATA L. MIERS) ETHANOLIC EXTRACT

2020 ◽  
pp. 236-240
Author(s):  
ERLINDHA GANGGA ◽  
YUNAHARA FARIDA ◽  
KARTININGSIH
Keyword(s):  
Antioxidant Activity ◽  
Microbial Contamination ◽  
Ph Value ◽  
Ethanolic Extract ◽  
Premature Aging ◽  
Phytochemical Screening ◽  
Active Ingredients ◽  
Greenish Yellow ◽  
Ic50 Value ◽  
Ethanolic Extracts

Objective: The aim of this study to develop an antioxidant gel from standardized green cincau (Cyclea barbata L. Miers) ethanolic extracts. Methods: The standardized extract of green cincau (Cyclea barbata L. Miers) ethanolic extracts were formulated as active ingredients of antioxidant gel. The dried leaf of Cyclea barbata L Miers was extracted with ethanol 70%. The extract was analyzed its phytochemical screening and antioxidant activity with DPPH methods. The extract was used as active ingredients of antioxidant gel. The gels were evaluated physically and chemically, such as organoleptic test, homogeneity, viscosity and rheology, spreadability, pH test, antioxidant activity, and analysis of microbial contamination test. Results: The results showed that all formulas were greenish-yellow and have homogeneous, the viscosity of 14267-45533 cps, spreadability of gels 1783.8–6631.5 mm2, pH value of 4.57–5.74, and analysis of microbial contamination has colonies not more than 103 colonies/g or colonies/ml. Statistical analysis used two ways ANOVA to investigate gels time and temperature effects toward gel preparations stability. There was no effect on the viscosity, spreadability, and pH tests, on the all formula with P>0.05. The antioxidant activity of the formula showed that all formulas changed after 3 mo of storage at 40 °C. Formula II had stronger antioxidant activity than the others with an IC50 value of 57.60µg/ml. Conclusion: All formulas showed fairly strong antioxidant activity because it can inhibit the activity of free radicals that could inhibit the premature aging of the skin.

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