Changes in the Technology of Soybean Production

Author(s):  
Dozet Gordana ◽  
Cvijanovic Gorica ◽  
Djukic Vojin

Nitrogen is the key element of yield and the most limiting factor in achieving high yields. Nitrogen fertilization is specific because mineral nitrogen, the available form of nitrogen for the plant in the soil, is on one hand subject to leaching losses due to its mobility in the soil and denitrification, and on the other hand to the content increase due to mineralization of soil organic matter. To encourage more intensive adoption of atmospheric nitrogen in nitrogen-fixing, the presence of cobalt and molybdenum is necessary. Molybdenum is required for the binding of atmospheric nitrogen by Azotobacter and plays an important role in the fixation of N2. Legumes treated with molybdenum have a larger amount of fixed nitrogen. Cobalt is relevant to the process of biological fixation of molecular nitrogen. The role of cobalt in biological fixation of molecular nitrogen is specific, and it cannot be replaced in the process by other trace elements. Inoculation of soybean seed with microbiological fertilizer and seed treatment with cobalt and molybdenum, as well as the use of corn crop fertilization with different doses of nitrogen, has a different impact on the yield and properties of soybeans.

2021 ◽  
Vol 74 (3) ◽  
pp. 9667-9674
Author(s):  
Luciano Moro ◽  
Maik Fernando Franz ◽  
Martios Ecco ◽  
Milciades Ariel Melgarejo Arrúa ◽  
Marlon Akiyama Ribas

Nitrogen is the element most demanded by the soybean crop, and the biological fixation of atmospheric nitrogen is the main means to supply it. In contrast, micronutrients and chemical treatments applied on seeds together with the inoculant can alter the phenomenon of biological fixation of atmospheric nitrogen. This work aimed to evaluate the effect of chemical products, micronutrients, and nitrogen fertilization on the nodulation, development, and yield of soybean. The experiment was developed in a field and a greenhouse in the municipality of Toledo, Brazil. A randomized block with four repetitions was used as an experimental design. This design had eight treatments, namely: T1 - Control (seeds treated with insecticide); T2 - Seeds treated with insecticides and inoculated with Bradyrhizobium; T3 - Untreated seeds inoculated with Bradyrhizobium; T4  Seeds treated with insecticides and cobalt-molybdenum (CoMo), inoculated with Bradyrhizobium; T5 - Seeds with CoMo inoculated with Bradyrhizobium; T6 - Seeds treated with insecticides, inoculated with Bradyrhizobium and with foliar application of CoMo; T7 - Seeds treated with insecticides, inoculated with Bradyrhizobium and with the application of nitrogen in cover; T8 - Seeds treated with nitrogen by broadcast. No significant differences were observed between treatments on the nodules numbers, stem diameter, plant height, root length, the mass of 1000 grains, and yield. The application of nitrogen at the R2 stage (a plant with an open flower in one of the two uppermost nodes of the main stem with a fully developed leaf) and in association with the inoculant + CoMo without seed treatment provided a greater number of nodes, pods, and grains per plant.


2019 ◽  
Vol 93 (3) ◽  
pp. 299
Author(s):  
Vanderlan Carneiro Dias ◽  
Joênes Mucci Peluzio ◽  
Maria Dilma De Lima

ABSTRACT  Due to its high economical importance and the need of high amounts of nitrogen fertilizer, the corn crop demands further and deeper studies on biological nitrogen fixation trough bacterium Azospirillum brasilense. Therefore, this study aimed at assessing influence of inoculation of three different genotypes (Orion, A1 Bandeirante and AG-1051) with such bacterium in association with cover application of five different nitrogen doses on percentage oil content in the grains. Data were subjected to ANOVA and means compared by Tukey test at 5%. The inoculation of A. brasilense, with or without nitrogen fertilization, has promoted oil content increase in the grains. Orion genotype presented the greatest increase with the bacterium inoculation, as well as it was the most productive at the lowest nitrogen dosages assessed. High temperatures favored the increase in the oil content in the grains. Keywords: Zea mays, inoculation, biological fixation, oil EFEITOS DA INOCULAÇÃO COM Azospirillum brasilense E DOSE DE NITROGÊNIO SOBRE O TEOR DE ÓLEO DE MILHO RESUMODevido à sua grande importância econômica e necessidade de fertilizantes nitrogenados, a cultura do milho exige estudos mais aprofundados sobre a fixação biológica do nitrogênio pela bactéria Azospirillum brasilense. Portanto, este estudo teve como objetivo avaliar a influência da inoculação de três diferentes genótipos (Orion, A1 Bandeirante e AG-1051) com essa bactéria em associação com a aplicação de cinco diferentes doses de nitrogênio no teor percentual de óleo nos grãos. Os dados foram submetidos à ANOVA e as médias comparadas pelo teste de Tukey a 5%. A inoculação de A. brasilense, com ou sem adubação nitrogenada, promoveu aumento do teor de óleo nos grãos. O genótipo Orion apresentou o maior aumento com a inoculação da bactéria, bem como o mais produtivo nas menores dosagens de nitrogênio avaliadas. As temperaturas altas favorecem ou aumentam o teor de óleo nos grãos.           Palavras-chave: Zea mays, inoculação, fixação biológica, óleo


2020 ◽  
Vol 637 ◽  
pp. 59-69 ◽  
Author(s):  
J Sullivan-Stack ◽  
BA Menge

Top predator decline has been ubiquitous across systems over the past decades and centuries, and predicting changes in resultant community dynamics is a major challenge for ecologists and managers. Ecological release predicts that loss of a limiting factor, such as a dominant competitor or predator, can release a species from control, thus allowing increases in its size, density, and/or distribution. The 2014 sea star wasting syndrome (SSWS) outbreak decimated populations of the keystone predator Pisaster ochraceus along the Oregon coast, USA. This event provided an opportunity to test the predictions of ecological release across a broad spatial scale and determine the role of competitive dynamics in top predator recovery. We hypothesized that after P. ochraceus loss, populations of the subordinate sea star Leptasterias sp. would grow larger, more abundant, and move downshore. We based these predictions on prior research in Washington State showing that Leptasterias sp. competed with P. ochraceus for food. Further, we predicted that ecological release of Leptasterias sp. could provide a bottleneck to P. ochraceus recovery. Using field surveys, we found no clear change in density or distribution in Leptasterias sp. populations post-SSWS, and decreases in body size. In a field experiment, we found no evidence of competition between similar-sized Leptasterias sp. and P. ochraceus. Thus, the mechanisms underlying our predictions were not in effect along the Oregon coast, which we attribute to differences in habitat overlap and food availability between the 2 regions. Our results suggest that response to the loss of a dominant competitor can be unpredictable even when based in theory and previous research.


2019 ◽  
Author(s):  
Andrew Romine ◽  
Kin Yang ◽  
Malkanthi Karunananda ◽  
Jason Chen ◽  
Keary Engle

A weakly coordinating monodentate heteroaryl thioether directing group has been developed for use in Pd(II) catalysis to orchestrate key elementary steps in the catalytic cycle that require conformational flexibility in a manner that is difficult to accomplish with traditional strongly coordinating directing groups. This benzothiazole thioether, (BT)S, directing group can be used to promote oxidative Heck reactivity of internal alkenes providing a wide range of products in moderate to high yields. To demonstrate the broad applicability of this directing group, arene C–H olefination was also successfully developed. Reaction progress kinetic analysis provides insights into the role of the directing group in each reaction, which is supplemented with computational data for the oxidative Heck reaction. Furthermore, this (BT)S directing group can be transformed into a number of synthetically useful functional groups, including a sulfone for Julia olefination, allowing it to serve as a “masked olefin” directing group in synthetic planning. In order to demonstrate this synthetic utility, natural products (+)-salvianolic acid A and salvianolic acid F are formally synthesized using the (BT)S directed C–H olefination as the key step.


Author(s):  
V.N. Zolotarev ◽  
◽  
I.S. Ivanov ◽  
O.N. Lyubtseva

Based on the analysis of data available in the literature and our own experimental material on phytocenotic selection of the stony stalk (Bromopsis inermis Holub.) the important role of competition between plants in the field for the creation of new varieties of perennial grasses that provide high yields of feed polyvid agrophytocenoses is shown.


Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1245
Author(s):  
Naoufal Lakhssassi ◽  
Valéria Stefania Lopes-Caitar ◽  
Dounya Knizia ◽  
Mallory A. Cullen ◽  
Oussama Badad ◽  
...  

Soybean is the second largest source of oil worldwide. Developing soybean varieties with high levels of oleic acid is a primary goal of the soybean breeders and industry. Edible oils containing high level of oleic acid and low level of linoleic acid are considered with higher oxidative stability and can be used as a natural antioxidant in food stability. All developed high oleic acid soybeans carry two alleles; GmFAD2-1A and GmFAD2-1B. However, when planted in cold soil, a possible reduction in seed germination was reported when high seed oleic acid derived from GmFAD2-1 alleles were used. Besides the soybean fatty acid desaturase (GmFAD2-1) subfamily, the GmFAD2-2 subfamily is composed of five members, including GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E. Segmental duplication of GmFAD2-1A/GmFAD2-1B, GmFAD2-2A/GmFAD2-2C, GmFAD2-2A/GmFAD2-2D, and GmFAD2-2D/GmFAD2-2C have occurred about 10.65, 27.04, 100.81, and 106.55 Mya, respectively. Using TILLING-by-Sequencing+ technology, we successfully identified 12, 8, 10, 9, and 19 EMS mutants at the GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E genes, respectively. Functional analyses of newly identified mutants revealed unprecedented role of the five GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E members in controlling the seed oleic acid content. Most importantly, unlike GmFAD2-1 members, subcellular localization revealed that members of the GmFAD2-2 subfamily showed a cytoplasmic localization, which may suggest the presence of an alternative fatty acid desaturase pathway in soybean for converting oleic acid content without substantially altering the traditional plastidial/ER fatty acid production.


2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Marian Nabil ◽  
Entesar E. Hassan ◽  
Neven S. Ghaly ◽  
Fawzia A. Aly ◽  
Farouk R. Melek ◽  
...  

Abstract Background The genus Albizia (Leguminoseae) is used in folk medicine for the treatment of a wide range of ailments. Recently, saponins from plant origin have attracted much attention. Saponins are recorded to have a broad range of biological and pharmacological activities. This study was performed to evaluate the protective role of Albizia chinensis bark methanolic extract (MEAC) against the genotoxicity induced by cyclophosphamide (CP) using different mutagenic parameters. Results The results showed that MEAC induced an inhibitory effect against chromosomal aberrations of CP in mouse bone marrow and spermatocytes. Such effect was found to be significant (p < 0.01) with a dose of 100 mg/kg treated once for 24 h and also after repeated treatment at a dose of 25 mg/kg for 7 days. In sperm abnormalities, the protective effect of Albizia extract showed a dose-related relationship. Different doses of MEAC (25, 50, and 100 mg/kg) significantly (p < 0.01) ameliorated sperm abnormalities induced by CP dose-dependently. The percentage of sperm abnormalities was decreased to 5.14 ± 0.72 in the group of animals treated with CP plus MEAC (100 mg/kg) indicating an inhibitory effect of about 50%. Conclusion MEAC at the doses examined was non-genotoxic compared to control (negative) and exhibited a protective role against CP genotoxicity.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shugang Zhao ◽  
Hongxia Wang ◽  
Kai Liu ◽  
Linqing Li ◽  
Jinbing Yang ◽  
...  

Abstract Background Tissue culture is an effective method for the rapid breeding of seedlings and improving production efficiency, but explant browning is a key limiting factor of walnut tissue culture. Specifically, the polymerization of PPO-derived quinones that cause explant browning of walnut is not well understood. This study investigated explants of ‘Zanmei’ walnut shoot apices cultured in agar (A) or vermiculite (V) media, and the survival percentage, changes in phenolic content, POD and PPO activity, and JrPPO expression in explants were studied to determine the role of PPO in the browning of walnut explants. Results The results showed that the V media greatly reduced the death rate of explants, and 89.9 and 38.7% of the explants cultured in V media and A media survived, respectively. Compared with that of explants at 0 h, the PPO of explants cultured in A was highly active throughout the culture, but activity in those cultured in V remained low. The phenolic level of explants cultured in A increased significantly at 72 h but subsequently declined, and the content in the explants cultured in V increased to a high level only at 144 h. The POD in explants cultured in V showed high activity that did not cause browning. Gene expression assays showed that the expression of JrPPO1 was downregulated in explants cultured in both A and V. However, the expression of JrPPO2 was upregulated in explants cultured in A throughout the culture and upregulated in V at 144 h. JrPPO expression analyses in different tissues showed that JrPPO1 was highly expressed in stems, young leaves, mature leaves, catkins, pistils, and hulls, and JrPPO2 was highly expressed in mature leaves and pistils. Moreover, browning assays showed that both explants in A and leaf tissue exhibited high JrPPO2 activity. Conclusion The rapid increase in phenolic content caused the browning and death of explants. V media delayed the rapid accumulation of phenolic compounds in walnut explants in the short term, which significantly decreased explants mortality. The results suggest that JrPPO2 plays a key role in the oxidation of phenols in explants after branch injury.


2021 ◽  
Vol 14 (6) ◽  
pp. 593
Author(s):  
Sandra Fernández-Rodríguez ◽  
Claudia Esposito-Zapero ◽  
Teodoro Zornoza ◽  
Ana Polache ◽  
Luis Granero ◽  
...  

N-acetylcysteine (NAC) is a prodrug that is marketed as a mucolytic agent and used for the treatment of acetaminophen overdose. Over the last few decades, evidence has been gathered that suggests the potential use of NAC as a new pharmacotherapy for alcohol use disorder (AUD), although its mechanism of action is already being debated. In this paper, we set out to assess both the potential involvement of the glutamate metabotropic receptors (mGluR) in the possible dual effect of NAC administered at two different doses and NAC’s effect on ethanol-induced activation. To this aim, 30 or 120 mg/kg of NAC was intraperitoneally administered to rats with the presence or absence of the negative allosteric modulator of mGluR5 (MTEP 0.1 mg/kg). Thereafter, the cFOS IR-cell expression was analyzed. Secondly, we explored the effect of 120 mg/kg of NAC on the neurochemical and behavioral activation induced by intra-VTA ethanol administration (150 nmol). Our results showed that the high NAC dose stimulated cFOS expression in the NAcc, and that this effect was suppressed in the presence of MTEP, thus suggesting the implication of mGluR5. Additionally, high doses could attenuate the ethanol-induced increase in cFOS-expression in the NAcc, probably due to a phenomenon based on the long-term depression of the MSNs. Additional experiments are required to corroborate our hypothesis.


2021 ◽  
Vol 127 (3) ◽  
Author(s):  
Umit Demirbas ◽  
Martin Kellert ◽  
Jelto Thesinga ◽  
Yi Hua ◽  
Simon Reuter ◽  
...  

AbstractWe present detailed experimental results with cryogenic Yb:YLF gain media in rod-geometry. We have comparatively investigated continuous-wave (cw) lasing and regenerative amplification performance under different experimental conditions. In the cw lasing experiments effect of crystal doping, cw laser cavity geometry and pump wavelength on lasing performance were explored. Regenerative amplification behavior was analyzed and the role of depolarization losses on performance was investigated. A recently developed temperature estimation method was also employed for the first time in estimating average crystal temperature under lasing conditions. It is shown that the thermal lens induced by transverse temperature gradients is the main limiting factor and strategies for future improvements are discussed. To the best of our knowledge, the achieved results in this study (375 W in cw, and 90 W in regenerative amplification) are the highest average powers ever obtained from this system via employing the broadband E//a axis.


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