scholarly journals Stevia rebaudiana (Bert) Bertoni: influence of osmotic stress and seed priming on seed germination under laboratory conditions

2017 ◽  
Vol 39 (3) ◽  
pp. 379 ◽  
Author(s):  
Keila Regina Hossa ◽  
José Walter Pedroza Carneiro ◽  
Terezinha Aparecida Guedes ◽  
Alessandro Lucca Braccini
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Stevia Rebaudiana ◽  
Seed Priming ◽  
Initial Time ◽  
Peg 6000 ◽  
Bedding Plants ◽  
Normal Seedling ◽  
First Results

 The foremost factor necessary for plant growers cultivating large acreages of Stevia rebaudiana (Bert) Bertoni is the production of qualitative bedding plants. The objective of this study was to evaluate the influence of osmotic-priming on the uniformity of seed germination. First, we evaluated the percentage of normal seedlings from two seed samples harvested in 2011 and 2012. The seeds harvested in 2012 produced 71.4% normal seedlings and thus they were used in the next experiments. The seeds were subjected to osmotic stress using five concentrations of polyethylene glycol (PEG-6000) at -0.2, -0.4, -0.6, -0.8, and -1.0 MPa in contrast with distilled water. Based on these first results, only -0.8 and -1.0 MPa were evaluated in the third experiment. The seeds were immersed in both concentrations of polyethylene glycol (PEG-6000) for imbibing at 20ºC for four, five, six, and seven days. Thereafter, we evaluated the time to the first normal seedling (Ti), time to the last normal seedling (Tf), percentage normal seedlings at the initial time (Pi) and percentage of normal seedlings at the end of every treatment (Pf). Osmotic priming increased the percentage of normal seedlings of the Stevia rebaudiana and reduced the time to the first and last germination events. 

Effect of Priming Techniques on Seed Germination Characteristics of C. Maxima Duch

2011 ◽  
Vol 474-476 ◽  
pp. 36-39 ◽  
Author(s):  
Yong Dong Sun ◽  
Xin Zheng Li ◽  
He Lian Yang ◽  
Li Sun
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Seed Priming ◽  
Distilled Water ◽  
Nacl Solution ◽  
Peg 6000 ◽  
Germination Process ◽  
Germination Characteristics ◽  
Successful Technique

The present study was conducted to investigate the effect of seed priming techniques on germination characteristics of C. maxima Duch. cultivar (Beiguan). Treatments were combinations of 3 levels of priming (distilled water, NaCl and PEG6000) and non-priming (control) with 3 replications. Concentrations of NaCl solution were 50, 100, 150, 200 mmol•L-1, and polyethylene glycol (PEG) 6000 were 10%, 20%, 30%, 40%, respectively. Seeds were primed using the above priming materials for 24 hours at 20°C in the dark, respectively. The results showed that different priming techniques could have various effects on germination of Beiguan seeds. Hydropriming (distilled water), NaCl priming and PEG6000 priming (10%) all improved the germination characteristics of Beiguan, compared to the control. NaCl priming was more effective than hydropriming and PEG6000 priming and was the most successful technique in this study. These findings indicated that seed priming techniques could accelerate germination process and were simple and cheap, we should propose these methods to farmers.

scholarly journals Effects of Osmopriming on Germination and Vigour Traits of Bersim Clover (Trifolium alexandrinum L.)

2010 ◽  
Vol 2 (4) ◽  
pp. 59-63 ◽  
Author(s):  
Hossein Reza ROUHI ◽  
Reza Tavakkol AFSHARI ◽  
Seyed Amir MOOSAVI ◽  
Mohammad Hossain GHARINEH
Keyword(s):  
Polyethylene Glycol ◽  
Osmotic Potential ◽  
Seed Priming ◽  
Control Group ◽  
Trifolium Alexandrinum ◽  
Distilled Water ◽  
Peg 6000 ◽  
Crop Establishment ◽  
Germination Characteristics ◽  
Polyethylene Glycol 6000

Previous studies suggested that fast and uniform germination is important for good crop establishment. The present study was conducted to investigate the possibility of increasing germination characteristics by seed priming techniques. An experiment was conducted with three replicates and two treatments including: 2 different priming duration (8 and 12 hours) and 6 osmotic potential of PEG 6000 solutions (-0.8, -1,-1.2,-1.4,-1.6 Mpa) and distilled water as a control group). The priming solutions were prepared using polyethylene glycol 6000 (PEG). Our results showed that the most effective osmotic potential in improving germination characteristics of Trifolium alexandrium is -0.8 MPa for 16 hours.

scholarly journals Ameliorative Effect of Seed Priming on Germination, Vigour Index and Tolerance Index against Short Term Moisture Stress in Maize (Zea mays L.)

2020 ◽  
Author(s):  
V. Singh ◽  
M. Sharma ◽  
H. Upadhyay ◽  
A. Siddique
Keyword(s):  
Polyethylene Glycol ◽  
Seed Priming ◽  
Moisture Stress ◽  
Magnesium Nitrate ◽  
Tolerance Index ◽  
Short Term ◽  
Seed Vigour ◽  
Peg 6000 ◽  
Vigour Index ◽  
Polyethylene Glycol 6000

An experiment was carried out to evaluate the effect of seed priming with magnesium nitrate salt against the moisture stress condition induced by Polyethylene glycol-6000 on seed germination, vigour index and tolerance index in root and shoot. Polyethylene glycol-6000 was used to create short term moisture stress under laboratory conditions while the seed priming was carried out by using magnesium nitrate. The percent germination, seed vigour index and tolerance index were started to decline from T1 to T4 (i.e. set first 1.5 and 4.5 % of PEG-6000 solution + none primed seed) while the results of second set (i.e. PEG-6000 + primed seed from T5 to T8) were also following the same decreasing trend along with improvement in every parameters. The germination was recorded highest 100 percent in T5 (i.e. 1.5% PEG-6000+ primed seed) followed by T6, T1 and T7 (T6 and T7 is 2.5% and 3.5 % PEG-6000+ primed seed) while the control set recorded 98.7% at final date of observations i.e. 240 Hrs. vigour index and tolerance index was also decreasing in both the set of treatments but PEG-600 + primed seed showed better results as compared to the treatments PEG-6000 + none primed. Tolerance index percent was recorded maximum in root as compare to shoot at 120 hrs. of intervals while the reverse result was recorded at 240 hrs. of intervals.

scholarly journals The Effect of Invigoration Using Polyethylene Glycol And Ultra Fine Bubble on Improving of Sengon Seeds (Falcataria Moluccana Miq.) Quality After Two Years Storage

Jurnal Wasian ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 133-143
Author(s):  
Iskandar Siregar ◽  
◽  
Riki Ramdhani ◽  
Evayusvita Rustam ◽  
Dede Sudrajat ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Seed Viability ◽  
Germination Capacity ◽  
Peg 6000 ◽  
Vigor Index ◽  
Fine Bubble ◽  
Initial Seed ◽  
Environmental Air

Utilization of seeds storage results in decreased plant productivity. The increase can be used by treating seeds before planting through the technique of invigoration. This purpose of the study is to assess the effectiveness of invigoration methods using polyethylene glycol (PEG 6000) and ultrafine bubbles (UFB) to improve viability and vigor of sengon seeds (Falcataria moluccana). Seed agieng using 96 % ethanol was carried out to obtain the diversity of seedlot viability as the materials for testing the effectiveness of invigoration treatments. A complete random design was used to test the effectiveness of 5 invigoration treatments, i.e., seed without invigoration treatment, soaking in PEG 6000 -0.8 Mpa, soaking in PEG 6000 -1.2 Mpa, soaking in UFB water injected by environmental air, and soaking in UFB water injected by oxygen 99 %, with soaking time is 24 hours for each treatment. Seed agieng resulted three classes of seed viability, i.e. 62 % (initial seed), 83 % (seed agieng for 30 minutes) and 57 % (seed agieng for 60 minutes). In the condition of seed germination before treatment (DBA) 57 % and DBA 62%, invigoration treatments were significantly affected on seed germination capacity, but not significantly different in DBA 83 %. The soaking treatment of UFB injected by oxigen 99 % was able to improve the germination parameters (germination capacity, germination rate, and vigor index) on the sengon seeds with DBA 57 %. For seeds with DBA 62 %, the soaking treatment in UFB injected by environmental air was provided the best germination capacity, T50, and vigor index. The treatment of UFB injected by oxygen 99 % was more effective to improve the seed with very low viability and vigor (DBA 57 %). In general, improving of seed viability and vigor is more effective by using UFB than PEG 6000.

scholarly journals Pattern of Capsule and Seed Development in Sesame (Sesamum indicum L.) as Influenced by Seed Priming

2020 ◽  
pp. 1-7
Author(s):  
Utpal Biswas ◽  
Rupa Das ◽  
Amitava Dutta
Keyword(s):  
Polyethylene Glycol ◽  
Seed Development ◽  
Seed Weight ◽  
Seed Priming ◽  
Quality Parameters ◽  
Steady Increase ◽  
Peg 6000 ◽  
Yield And Quality ◽  
Main Plot ◽  
Days After Anthesis

Aim: To study the influence of seed priming on the pattern of capsule and seed development in sesame. Place and Duration of Study: The field experiment was conducted during the pre kharif seasons of 2017-2018 and 2018-2019 in sesame variety Savitri at AB Block farm, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia, West Bengal, India. Methodology: Experiment was laid out in split plot design with 3 replications. Ten schedules of seed priming viz T1 (KNO3 @ 10 mM), T2 (KNO3 @ 20 mM), T3 (KNO3 @ 50 mM), T4 (KH2PO4 @ 50 mM), T5 (KH2PO4 @ 100 mM),T6 (KH2PO4 @ 200 mM), T7 [Polyethylene glycol (PEG) 6000 @ -0.4 MPa], T8 [Polyethylene glycol (PEG) 6000 @ -0.3 MPa], T9 [Polyethylene glycol (PEG) 6000 @ -0.2 MPa], T10 Distilled water (Hydro priming) along with control T11 (Dry seed) were taken as main plot treatment and stage of harvest was considered as sub plot treatment. The pattern of capsule and seed development was studied at 10 days after anthesis (DAA), 20 days after anthesis (DAA), 30 days after anthesis (DAA), 40 days after anthesis (DAA) and 50 days after anthesis (DAA) interval. Ten plants from each replication and in each treatment were selected at random to record data on morphological and physiological characters. Results: Fresh capsule length, fresh capsule breadth, fresh capsule weight, fresh seed weight and dry seed weight showed a steady increase up to 40 days after anthesis (DAA) then decreased slowly up to maturity. Conclusion: Considering seed yield and quality parameters, T7 [Polyethylene glycol (PEG) 6000 @ -0.4 MPa] and T9 [Polyethylene glycol (PEG) 6000 @ -0.2 MPa] appears to be ideal among the treatments for quality seed production in sesame.

scholarly journals The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 94
Author(s):  
Iman Mirmazloum ◽  
Attila Kiss ◽  
Éva Erdélyi ◽  
Márta Ladányi ◽  
Éva Zámboriné Németh ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Petri Dish ◽  
Seed Priming ◽  
Germination Percentage ◽  
Positive Effects ◽  
Carum Carvi ◽  
Early Seedling ◽  
Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

scholarly journals Effects of Osmotic Stress Induced by Polyethylene Glycol (Peg) 6000 and Mannitol on Seed Germination and Seedling Growth of Durum Wheat

2021 ◽  
Vol 8 (3) ◽  
pp. 57-66
Author(s):  
Ratiba Bousba ◽  
Rabah Bounar ◽  
Narimene Sedrati ◽  
Randa Lekhal ◽  
Chourouk Hamla ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Durum Wheat ◽  
Crop Production ◽  
Germination Rate ◽  
Inhibitory Effect ◽  
Wheat Genotype ◽  
Peg 6000 ◽  
Germination Parameters

Seed germination is generally the critical step in seed establishment and thus the determination of successful crop production. This study was focused at examination of the biochemical and germination parameters effected by low water potential which was generated by polyethylene glycol (PEG) 6000 and mannitol, related to drought stress and growth of Waha durum wheat genotype. Two tests were carried out in a growth chamber; the first comprises seed germination into Petri dishes in the presence of different concentrations of the two osmoticums (0, 5, 10, 15 and 20 % of PEG6000 and mannitol). The second test was carried out in nutrient solution BD medium. Our results shows that Both PEG -6000 and mannitol reduced germination. Therefore, a rapid increase was observed in the rate of germination both for the control plants and the plants subjected to a concentration of 5 g/L and 10 g/L and changes in proportion to the time. For the concentration of 15 g/L and 20 g/L, this phase is very short, which explains the reduced germination rate due to the inhibitory effect of the two osmoticums on germination. In this study, PEG-6000 treatments resulted in an increase of some proteins and a decrease of others. Waha displayed 12 bands for control plants, 40 bands for PEG-6000 stressed plants (all treatments) and 35 bands for mannitol treatments.

scholarly journals Influence of PEG generated osmotic stress on shoot regeneration and some biochemical parameters in Lathyrus culture

2014 ◽  
Vol 50 (No. 2) ◽  
pp. 77-83 ◽  
Author(s):  
B. Piwowarczyk ◽  
I. Kamińska ◽  
W. Rybiński
Keyword(s):  
Water Stress ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Shoot Regeneration ◽  
Biochemical Parameters ◽  
Multiplication Rate ◽  
Crop Species ◽  
Peg 6000 ◽  
Agronomic Potential ◽  
The Media

Several Lathyrus species and in particular Lathyrus sativus (grass pea) display much tolerance to drought and have great agronomic potential as grain and forage legumes. Studies on these crop species could improve our understanding of the mechanisms of plant resistance to water stress. This investigation was aimed at examining the effect of low water potential generated by polyethylene glycol (PEG) 6000 on seed germination, shoot regeneration and some biochemical parameters related to drought stress. Seeds of eight Lathyrus accessions (six of L. sativus, one of L. cicera, one of L. tingitanus) were cultured on MB (MS macro and microelements with B5 vitamins) medium with addition of 5 mg/l BAP (6-benzyloaminopurine). Different levels of water-stress were induced by using three concentrations of PEG 6000 (50, 100 and 150 g/l) in MB medium. Osmotic stress had no influence on seed germination of any of the accessions tested. However, an increased concentration of PEG in the media caused a decrease in the multiplication rate and vigour of newly regenerated shoots. The percentage content of dry matter and proline content in Lathyrus shoots increased significantly under osmotic stress. In turn, water stress had a little effect on photosynthetic pigments.

scholarly journals Multi-Step Cellular and Tissue Selection of Soybean for Resistance to Osmotic Stress Using Peg 6000 Invitro Conditions

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Mohammad Naim Noori
Keyword(s):  
Polyethylene Glycol ◽  
Osmotic Stress ◽  
Nutrient Medium ◽  
Selective Agent ◽  
Soybean Variety ◽  
Peg 6000 ◽  
Final Volume ◽  
Polyethylene Glycol 6000 ◽  
Selection Of

Cell and tissue selection in vitro allows targeted selection of the desired traits under severe selective conditions at the level of individual cells and tissues. On the basis of multistage cell and tissue selection with the use of a selective agent - neutral osmotic polyethylene glycol 6000 in increasing concentrations -5%, 10%, 15%, 20% of the final volume of the nutrient medium, callus of the Zhansaya soybean variety that are stably resistant to osmotic stress have been obtained. Regeneration from callus was noted only in8.3 % of the planted callus 6 soybean regenerants plants resistant to osmotic stress were obtained from callus.

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