Study on Combination and Bioremediation of Chlorimuron-Ethyl-Degrading Strains

2011 ◽  
Vol 340 ◽  
pp. 215-221 ◽  
Author(s):  
Chun Hong Xu ◽  
Chun Yan Li ◽  
Ming Hua Xiong ◽  
Jun Bo Pan ◽  
Xue Song Li ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Stenotrophomonas Maltophilia ◽  
Removal Rate ◽  
Inoculum Size ◽  
Orthogonal Test ◽  
Cucumber Seedling ◽  
Rhodotorula Mucilaginosa ◽  
Culture Experiment ◽  
Chlorimuron Ethyl ◽  
Pot Culture

The degradation capacity of the four chlorimuron-ethyl-degrading strains, D310-1, LCY-2, LCY-3 and LCY-4, which identified asRhodococcussp.,Stenotrophomonas maltophilia sp,Bacillus subtilis sp., andRhodotorula mucilaginosasp., respectively, combined in different ways was examined in this study. Then the optimal degradation conditions and mitigation phytotoxicity of chlorimuron-ethyl on sensitive crop were investigated by both orthogonal test and pot culture experiment. The experimental results showed that the combination of four strains was adopted, and the removal rate of chlorimuron-ethyl reached the maximum of 90% in 30 days. Then the optimal degradation conditions were inoculum size 2.5%, temperature 28°C, pH 7.0 and the soil moisture 35%, and the mixed strains could eradicate 92.57% of chlorimuron-ethyl within 30 days. Furthermore pot culture experiment indicated that inoculation of mixed strains could also mitigate the phytotoxic effects of chlorimuron-ethyl on the growth of cucumber seedling.

scholarly journals Isolation and Optimal Fermentation Condition of the Bacillus subtilis Subsp. natto Strain WTC016 for Nattokinase Production

Fermentation ◽  
2019 ◽  
Vol 5 (4) ◽  
pp. 92 ◽  
Author(s):  
Shouyong Ju ◽  
Zhilin Cao ◽  
Christina Wong ◽  
Yangyang Liu ◽  
Mohamed F. Foda ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Bacillus Subtilis ◽  
Fibrinolytic Activity ◽  
Inoculum Size ◽  
Orthogonal Test ◽  
Fermentation Condition ◽  
Fermentation Conditions ◽  
Optimal Tests ◽  
Physiological Tests ◽  
Subtilis Subsp

Nattokinase is a serine protease in the subtilisin family which is produced by Bacillus subtilis subsp. natto and exhibits vigorous fibrinolytic activity that has been suggested to be able to prevent and treat thromboembolic diseases. In this study, WTC016, a spore-forming and rod-shaped bacterium with fibrinolytic activity was successfully isolated from soil, which was identified as Bacillus subtilis subsp. natto based on morphological and physiological tests, and phylogenetic analysis of 16S rRNA and gyrA. According to the growth curve of WTC016, the nattokinase production reached the highest amount in the stationary phase. To optimize the liquid fermentation condition for nattokinase yield of WTC016, further optimal tests of four factors, including the temperature, pH, inoculum size, and loading volume, followed by orthogonal test of all these factors, was performed. The optimal fermentation conditions were determined as 30 °C, 7.0 pH, 2% inoculum size, and 60 mL of loading volume in 250 mL conical flask, which indicates the highest nattokinase production of 3284 ± 58 IU/mL while fermented for 26 h. This work laid the foundation for producing nattokinase using Bacillus subtilis subsp. natto WTC016.

scholarly journals Prospective Zinc Solubilising Bacteria for Enhanced Nutrient Uptake and Growth Promotion in Maize (Zea maysL.)

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Praveen Kumar Goteti ◽  
Leo Daniel Amalraj Emmanuel ◽  
Suseelendra Desai ◽  
Mir Hassan Ahmed Shaik
Keyword(s):  
Plant Growth ◽  
Solid Medium ◽  
Growth Promotion ◽  
Dry Mass ◽  
Short Term ◽  
Culture Experiment ◽  
Zinc Supplement ◽  
Seed Bacterization ◽  
Significant Release ◽  
Pot Culture

Zinc (Zn) is one of the essential micronutrients required for optimum plant growth. Substantial quantity of applied inorganic zinc in soil is converted into unavailable form. Zinc solubilising bacteria are potential alternates for zinc supplement. Among 10 strains screened for Zn solubilisation, P29, P33, and B40 produced 22.0 mm clear haloes on solid medium amended with ZnCO3. Similarly, P17 and B40 showed 31.0 mm zone in ZnO incorporated medium. P29 and B40 showed significant release of Zn in broth amended with ZnCO3(17 and 16.8 ppm) and ZnO (18 and 17 ppm), respectively. The pH of the broth was almost acidic in all the cases ranging from 3.9 to 6.1 in ZnCO3and from 4.1 to 6.4 in ZnO added medium. Short term pot culture experiment with maize revealed that seed bacterization with P29 @ 10 g·kg−1significantly enhanced total dry mass (12.96 g) and uptake of N (2.268%), K (2.0%), Mn (60 ppm), and Zn (278.8 ppm).

scholarly journals Nitrogen fixating ability of mungbean genotypes under different levels of nitrogen application

2016 ◽  
Vol 41 (1) ◽  
pp. 163-171 ◽  
Author(s):  
MA Razzaque ◽  
MM Haque ◽  
MA Karim ◽  
ARM Solaiman
Keyword(s):  
Nitrogen Fixation ◽  
Seed Yield ◽  
Biological Nitrogen Fixation ◽  
Yield Potential ◽  
Nitrogen Application ◽  
Culture Experiment ◽  
N Application ◽  
Biological Nitrogen ◽  
Different Levels ◽  
Pot Culture

A pot culture experiment was conducted at the Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur during kharif II, 2012 to evaluate the nodulation, biological nitrogen fixation and yield potential of genotypes of mungbean under varying levels of N application. There were 10 mungbean genotypes viz. IPSA 12, GK 27, IPSA 3, IPSA 5, ACC12890055, GK 63, ACC12890053, BU mug 4, BARI Mung 6 and Binamoog 5, each genotype treated with six levels of N (0, 20, 40, 60, 80 and 100 kg N ha-1) . Among the genotypes, the IPSA 12 at 40 kg N ha-1 produced the maximum number of nodules (14.54 plant-1) as well as the highest nitrogen fixation (2.684 mmol C2H4). This resulted in the highest seed yield (14.22 g plant-1). The genotype ACC12890053 recorded the lowest nodulation (6 plant-1), nitrogen fixation (1.134) and seed yield (7.33 g plant-1).Bangladesh J. Agril. Res. 41(1): 163-171, March 2016

Interaction between Glomus fasciculatum and Sclerotium rolfsii in peanut

1983 ◽  
Vol 61 (9) ◽  
pp. 2349-2351 ◽  
Author(s):  
K. R. Krishna ◽  
D. J. Bagyaraj
Keyword(s):  
Mycorrhizal Fungus ◽  
Sclerotium Rolfsii ◽  
Glomus Fasciculatum ◽  
Root Infection ◽  
Culture Experiment ◽  
Root Pathogen ◽  
Simultaneous Addition ◽  
Severity Of Disease ◽  
P Content ◽  
Pot Culture

The interaction between the mycorrhizal fungus Glomus fasciculatum and the root pathogen Sclerotium rolfsii was studied in a pot culture experiment. The mycorrhizal fungus reduced the number of sclerotia produced by the pathogen and the pathogen reduced the percentage root infection and chlamydospore production by the mycorrhizal fungus. Root and shoot dry weights of the host and their P content was highest in plants inoculated with mycorrhiza only and lowest in plants inoculated with the pathogen only. Simultaneous addition of mycorrhizal inoculum and the pathogen reduced the severity of disease.

Hyperaccumulative property comparison of 24 weed species to heavy metals using a pot culture experiment

2008 ◽  
Vol 152 (1-4) ◽  
pp. 299-307 ◽  
Author(s):  
Shuhe Wei ◽  
Qixing Zhou ◽  
Hong Xiao ◽  
Chuanjie Yang ◽  
Yahu Hu ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Weed Species ◽  
Culture Experiment ◽  
Pot Culture

scholarly journals RHIZODEGRADATION OF PHENANTHRENE, ANTHRACENE AND PYRENE BY AUGMENTING BACILLUS CEREUS AND BACILLUS SUBTILIS STRAINS

2021 ◽  
Vol 9 (02) ◽  
pp. 89-96
Author(s):  
M. Poornachander Rao ◽  
◽  
Anitha Yerra ◽  
K. Satyaprasad ◽  
◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Contaminated Soils ◽  
Rhizosphere Soil ◽  
Culture Method ◽  
Experimental Period ◽  
Bacterial Strains ◽  
Bacterial Populations ◽  
Metabolic Activities ◽  
Pot Culture

Rhizodegradation is one of the best methods for the effective removal of dangerous polycyclic aromatic hydrocarbons pollutants from soil. This is operative due to the high persistent, non-bioavailability nature of PAHs and combined, sequential reactions of bacteria present in rhizosphere of plants. We have conducted pot-culture method to study the degradation of three PAHs compounds namely phenanthrene, anthracene and pyrene in artificially contaminated soils of rhizosphere and non-rhizosphere soil treatments of blackgram(Vignamungo L.) that augmented by two potential PAHs degraders namely Bacillus cereus CPOU13 and Bacillus subtilis SPC14 isolated from naturally contaminated soils for 90days. HPLC studies revealed that degradation percentages of the three PAHs in treatments were more where selected strains augmented to the soil treatments over the non-augmented soils. The rhizosphere treatments that have augmented strains recorded more degradation percentages of phenanthrene, anthracene and pyrene over the rhizosphere treatments that were non-augmented. Pyrene, a high molecular weight PAHs degraded maximum to 96.24% in rhizosphere soil treatment that is augmented with the strains while moderate degradation of pyrene recorded in non-autoclaved soil treatments that contain natural microbial communities. The study of counting of bacterial populations during the experimental period revealed that the populations of the selected and other natural bacteria were gradually increased from the first day, reached maximum by 60days and became almost consistent in 90days in all the treatments. It was also observed that the populations of bacteria were high in rhizosphere treatments compared to the non-rhizosphere soil treatments. With these results it has been predicted that degradation of PAHs in rhizosphere soil treatments is closely associated with the increasing PAHs degrading bacterial populations of selected bacterial strains that may consume more quantity of PAHs for their metabolic activities in rhizosphere soils. Key words: Rhizodegradation, PAHs, HPLC, pot culture.

scholarly journals Bio-degradation of Azo Dye Acid Orange-10 by a New Isolate of Bacillus subtilis Isolated from Soil Sample around Textile Industry in South Karnataka

2021 ◽  
Vol 17 (4) ◽  
pp. 707-716
Author(s):  
Hema Gunti ◽  
Vyshali Venkatappa Maruthiramaih ◽  
Tippeswamy Boreddy Shivanandappa
Keyword(s):  
Bacillus Subtilis ◽  
Textile Industry ◽  
Azo Dye ◽  
Industrial Effluent ◽  
Inoculum Size ◽  
Soil Samples ◽  
Synthetic Dyes ◽  
Acid Orange ◽  
Acid Orange 10 ◽  
Static Conditions

Untreated effluents from the textile industry affect aquatic life irreversibly. Synthetic dyes not only change the color of water resources but also make them hazardous.The main objective of the study was to evaluate the decolorizing potential of a new isolate of Bacillus subtilis from soil samples contaminated with industrial effluent in and around textile industrial area in South Karnataka. This isolate of Bacillus subtilis has high decolorizing potential and took only 24 hrs for complete decolorization of acid orange-10 azo dye at 200ppm. Different parameters like temperature, pH, aeration, dye concentration and inoculum size were optimized for complete decolorization of Acid orange-10 azo dye by this isolate of Bacillus subtilis. The dye was completely decolorized at 400C within 24 hrs and it was capable of decolorizing 700 ppm dye in 72 hrs. Optimum pH was found to be 8.5 and maximum decolorization was achieved under static conditions. As the inoculum size increased, the time taken for complete decolorization of Acid orange-10 dye was decreased from 36 hrs at 1% to 16 hrs at 10% of inoculum size. The new isolate decolorizes 100 ppm of dye completely (i.e.100%) within 12hrs of incubation. The time taken for the complete decolorization increased with increase in the concentration of Acid orange-10 azo dye. In conclusion, the new isolate of Bacillus subtilis from soil samples contaminated with textile industrial effluent was found to be a potential candidate for decolorization of Acid orange-10 azo dye in textile effluents.

scholarly journals DEVELOPMENT AND TESTING OF SOIL IMPURITIES REMOVING APPARATUS FOR POTATO

2021 ◽  
pp. 485-494
Author(s):  
Hongguang Yang ◽  
Huanxiong Xie ◽  
Hai Wei ◽  
Jianchun Yan ◽  
Huichang Wu ◽  
...  
Keyword(s):  
Optimal Parameter ◽  
Removal Rate ◽  
Orthogonal Test ◽  
Movement Speed ◽  
Deep Processing ◽  
Cleaning Operation ◽  
Damage Rate ◽  
Influencing Parameters ◽  
Impurities Removal ◽  
Average Damage

Aiming at the problem that contain more soil impurities of potato post-harvest, which affects subsequent deep processing, an apparatus for removing soil impurities from potato was developed. The whole structure is mainly composed of frame, feeding port, flexible rubber finger conveying mechanism, slender filament rotary brushing mechanism, discharging port and the like. The research and analysis determined that the main parameters influencing the soil impurities removal performance were the movement speed of conveying mechanism, the movement speed of brushing mechanism and the clearance between conveying mechanism and brushing mechanism (hereinafter referred to as the mechanism clearance). Taking the main influencing parameters as test factors, and the soil impurities removal rate and potato damage rate as indexes, the orthogonal test with three factors and three levels was carried out. The optimal parameter combination was obtained as follows: the movement speed of conveying mechanism was 0.35m/s, the movement speed of brushing mechanism was 0.40m/s, and the mechanism clearance was 55mm. At this time, the average soil impurities removal rate was 87.18%, and the potato average damage rate was 1.95%, which met the requirements of potato cleaning operation.

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