Covalently Crosslinked Human-Like Collagen Hydrogel: Properties of Biocompatibility

2012 ◽  
Vol 550-553 ◽  
pp. 1114-1119
Author(s):  
Yu Zhang Du ◽  
Dai Di Fan ◽  
Xiao Xuan Ma ◽  
Chen Hui Zhu ◽  
Li Jun Zhang
Keyword(s):  
Cell Adhesion ◽  
Inflammatory Response ◽  
Mtt Assay ◽  
Carboxymethyl Chitosan ◽  
Cross Linking ◽  
Bhk21 Cell ◽  
Tetrazolium Bromide ◽  
Collagen Hydrogel ◽  
The Cross ◽  
Cell Adhesion And Proliferation

In this paper, the cross-linking injection hydrogel were synthesized by EDC crosslinker and Carboxymethyl chitosan (CMCS)/Human-like collagen (HLC). Cytotoxicity was assessed by Methylthiazolydiphenyl-tetrazolium bromide (MTT) assay which indicated that the hydrogels was non-toxic to the BHK21 cell .Chondrocyct-encapsulation of this hydrogel were studied in order to asses the cells compatibility of the injection gel. The result showed that the material has no cytotoxicity to the cells and promoted cell adhesion and proliferation. Injected those hydrogels into mice subcutaneous , the following parameters were evaluated: inflammatory response, vascularization, new hypoderm generation. After 2,4,12,and 24 weeks of healing, the rats were sacrilifced suggested that gels in animals did not induce inflammation obvious, vessel bestrid the material after 4 weeks injection, new hypoderm generated in 12 weeks and packaged the hydrogels after 24 weeks. Consequently the gels are promised for the application in the biomaterials area.

Cell adhesion and proliferation enhancement by gelatin nanofiber scaffolds

2011 ◽  
Vol 26 (6) ◽  
pp. 565-577 ◽  
Author(s):  
Shih-Ching Wu ◽  
Wei-Hong Chang ◽  
Guo-Chung Dong ◽  
Kuo-Yu Chen ◽  
Yueh-Sheng Chen ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cost Effective ◽  
Average Diameter ◽  
Gelatin Film ◽  
Cross Linking ◽  
Electrospinning Technique ◽  
Cell Compatibility ◽  
The Cross ◽  
Electrospun Gelatin ◽  
Cell Adhesion And Proliferation

Gelatin nanofibers (GNs) prepared by electrospinning were cross-linked with glutaraldehyde vapor to improve their water-resistant ability. After cross-linking treatment, the form of the fibers expressed no substantial change, but the average diameter of the fibers increased with increasing cross-linking time. The swelling induced by the moisture during the cross-linking process was moderated when the cross-linking time reached 45 min. The contact angle measurements confirmed that the electrospun gelatin fibers were more hydrophilic than the gelatin film (GF). Increasing the cross-linking time did not alter the hydrophilic properties of the gelatin fibers. The cell compatibility was evaluated based on 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay, scanning electron microscope and confocal microscope observations, and Western blot analysis by culturing MG-63 cells on the GFs and GNs. The nanofibrous structure fabricated by an electrospinning technique was found to enhance cell adhesion and proliferation. This process is a cost-effective simulation of GN structures’ promising applications on scaffold preparation for tissue engineering.

Multiple Cross-Linking of a Small Peptide to Form a Size Tunable Biopolymer with Efficient Cell Adhesion and Proliferation Property

2018 ◽  
Vol 19 (10) ◽  
pp. 3994-4002 ◽  
Author(s):  
Payel Dowari ◽  
Shriya Saha ◽  
Bapan Pramanik ◽  
Sahnawaz Ahmed ◽  
Nilotpal Singha ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cross Linking ◽  
Small Peptide ◽  
Multiple Cross ◽  
Cell Adhesion And Proliferation

scholarly journals Novel Scaffolds Fabricated Using Oleuropein for Bone Tissue Engineering

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Hui Fan ◽  
Junfeng Hui ◽  
Zhiguang Duan ◽  
Daidi Fan ◽  
Yu Mi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Mechanical Tests ◽  
Cell Counting ◽  
Cross Linking ◽  
The Cross ◽  
Cell Adhesion And Proliferation

We investigated the feasibility of oleuropein as a cross-linking agent for fabricating three-dimensional (3D) porous composite scaffolds for bone tissue engineering. Human-like collagen (HLC) and nanohydroxyapatite (n-HAp) were used to fabricate the composite scaffold by way of cross-linking. The mechanical tests revealed superior properties for the cross-linked scaffolds compared to the uncross-linked scaffolds. The as-obtained composite scaffold had a 3D porous structure with pores ranging from 120 to 300 μm and a porosity of73.6±2.3%. The cross-linked scaffolds were seeded with MC3T3-E1 Subclone 14 mouse osteoblasts. Fluorescence staining, the Cell Counting Kit-8 (CCK-8) assay, and scanning electron microscopy (SEM) indicated that the scaffolds enhanced cell adhesion and proliferation. Our results indicate the potential of these scaffolds for bone tissue engineering.

Optimization of Scaffold for a Successful Hydrogel-Seeding Method for Vascular Tissue Engineering

2007 ◽  
Vol 342-343 ◽  
pp. 333-336 ◽  
Author(s):  
In Su Park ◽  
Sang Heon Kim ◽  
Jeong Woo Han ◽  
Young Gun Ko ◽  
Eun Na Chung ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Pore Size ◽  
Vascular Tissue ◽  
Porous Scaffolds ◽  
Cell Seeding ◽  
Established Method ◽  
Collagen Hydrogel ◽  
Seeding Method ◽  
Elastic Polymer ◽  
Cell Adhesion And Proliferation

Porosity and pore size are needed for successful cell seeding and proliferation into porous scaffolds. This study was focused on a hydrogel-seeding method to improve cell adhesion and proliferation in tubular porous scaffolds for vascular grafts application. Tubular scaffolds were fabricated from a biodegradable elastic polymer, poly(L-lactide-co-ε-caprolactone) (PLCL) (50:50, Mn 1.58×105), by an extrusion-particulate leaching method. Vascular smooth muscle cells (VSMCs) were dispersed in collagen hydrogel and then seeded into the tubular PLCL scaffolds having various pore sizes, 50-100 μm, 100-200 μm, and 300-500 μm, respectively. As a result, the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds. Especially, the cell proliferation efficiency was improved by using the hydrogel-seeding method as compared with by using a previously established method. In summary, this study demonstrates that the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds in the hydrogel-seeding method.

Study the Biodegradation of a Injection Human-Like Collagen Hydrogel

2012 ◽  
Vol 535-537 ◽  
pp. 2291-2295 ◽  
Author(s):  
Yu Zhang Du ◽  
Dai Di Fan ◽  
Xiao Xuan Ma ◽  
Chen Hui Zhu ◽  
Li Jun Zhang
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Carboxymethyl Chitosan ◽  
Cross Linking ◽  
In Vitro Degradation ◽  
Collagen Hydrogel ◽  
Degradation Time ◽  
Scanning Electron

In this paper, the cross-linking hydrogels were synthesized by EDC crosslinker and Carboxymethyl chitosan (CMCS)/Human-like collagen (HLC). The morphology of the hydrogels was observed by scanning electron microscope (SEM). The important performance of these hydrogels is biodegradation which was assessed in two ways: (1) Through enzyme solution experiment in vitro , investigating in vitro degradation property. (2) Injecting the hydrogels to mice subcutaneous , after 2,4,12,and 24 weeks of healing ,the rats were sacrificed and explanted specimens were prepared for histology analysis. Investigating degradation time in vitro and in vivo , and studying the histology .HLC hydrogels degradation time gets eight to ten months, in addition has good compatibility of the histological performances. The hydrogels are promised for the applications in the biomaterials area.

scholarly journals Investigation of the functional network modifier loading on the stoichiometric ratio of epoxy resins and their dielectric properties

2021 ◽  
Author(s):  
Istebreq A. Saeedi ◽  
Sunny Chaudhary ◽  
Thomas Andritsch ◽  
Alun S. Vaughan
Keyword(s):  
Glass Transition ◽  
Dielectric Properties ◽  
Electrical Properties ◽  
Epoxy Resins ◽  
Functional Network ◽  
Cross Linking ◽  
Network Modifier ◽  
Epoxy Resin System ◽  
The Cross ◽  
The Impact

AbstractReactive molecular additives have often been employed to tailor the mechanical properties of epoxy resins. In addition, several studies have reported improved electrical properties in such systems, where the network architecture and included function groups have been modified through the use of so-called functional network modifier (FNM) molecules. The study reported here set out to investigate the effect of a glycidyl polyhedral oligomeric silsesquioxane (GPOSS) FNM on the cross-linking reactions, glass transition, breakdown strength and dielectric properties of an amine-cured epoxy resin system. Since many previous studies have considered POSS to act as an inorganic filler, a key aim was to consider the impact of GPOSS addition on the stoichiometry of curing. Fourier transform infrared spectroscopy revealed significant changes in the cross-linking reactions that occur if appropriate stoichiometric compensation is not made for the additional epoxide groups present on the GPOSS. These changes, in concert with the direct effect of the GPOSS itself, influence the glass transition temperature, dielectric breakdown behaviour and dielectric response of the system. Specifically, the work shows that the inclusion of GPOSS can result in beneficial changes in electrical properties, but that these gains are easily lost if consequential changes in the matrix polymer are not appropriately counteracted. Nevertheless, if the system is appropriately optimized, materials with pronounced improvements in technologically important characteristics can be designed.

scholarly journals The Effect of Surfactant-Modified Montmorillonite on the Cross-Linking Efficiency of Polysiloxanes

Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2623
Author(s):  
Monika Wójcik-Bania ◽  
Jakub Matusik
Keyword(s):  
Total Pore Volume ◽  
Cross Linking ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
The Cross ◽  
Chain Lengths ◽  
First Time ◽  
Substituent Influence ◽  
Benzyl Substituent

Polymer–clay mineral composites are an important class of materials with various applications in the industry. Despite interesting properties of polysiloxanes, such matrices were rarely used in combination with clay minerals. Thus, for the first time, a systematic study was designed to investigate the cross-linking efficiency of polysiloxane networks in the presence of 2 wt % of organo-montmorillonite. Montmorillonite (Mt) was intercalated with six quaternary ammonium salts of the cation structure [(CH3)2R’NR]+, where R = C12, C14, C16, and R’ = methyl or benzyl substituent. The intercalation efficiency was examined by X-ray diffraction, CHN elemental analysis, and Fourier transform infrared (FTIR) spectroscopy. Textural studies have shown that the application of freezing in liquid nitrogen and freeze-drying after the intercalation increases the specific surface area and the total pore volume of organo-Mt. The polymer matrix was a poly(methylhydrosiloxane) cross-linked with two linear vinylsiloxanes of different siloxane chain lengths between end functional groups. X-ray diffraction and transmission electron microscopy studies have shown that the increase in d-spacing of organo-Mt and the benzyl substituent influence the degree of nanofillers’ exfoliation in the nanocomposites. The increase in the degree of organo-Mt exfoliation reduces the efficiency of hydrosilylation reaction monitored by FTIR. This was due to physical hindrance induced by exfoliated Mt particles.

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