Bio-Treatment of Crude Oil Polluted Water Using Mixed Microbial Populations of P. Aureginosa, Penicillium Notatum, E. Coli and Aspergillus Niger

2009 ◽  
Vol 62-64 ◽  
pp. 802-807
Author(s):  
K.O. Obahiagbon ◽  
C.N. Owabor
Keyword(s):  
Aspergillus Niger ◽  
Crude Oil ◽  
Microbial Population ◽  
Oil Spills ◽  
Microbial Populations ◽  
Polluted Water ◽  
Penicillium Notatum ◽  
E Coli ◽  
Hydrocarbon Content ◽  
Total Hydrocarbon Content

Bioremediation as a strategy of clean- up of crude oil spills in water using a mixed microbial population of E. Coli, Aspergillus niger, Penicillum notatum and Pseudomonas aeruginosa was investigated in this study. The sample of crude oil polluted water containing the mixed microbes and the control was monitored for parameters such as BOD, pH, Total Hydrocarbon Content (THC) and Turbidity over a period of nine weeks. At the end of the bioremediation period, the results obtained showed that the sample remediated with the mixed microbes gave a THC drop from 14975mg/l -1.960mg/l (99.9% drop) compared to the control with a drop from 14975 – 18.72mg/l (99.8% drop). A BOD drop from 1447.4mg/l – 17.1mg/l (98.8?% drop) for the sample mixed microbes and 1447.4 – 90.8mg/l (93.7% drop) for the control. Turbidity values increased from 5.0 NTU – 25.2NTU for sample with mixed microbes and 5.0 NTU – 23.1 NTU for the control. The values of THC (1.96 mg/l) and BOD (17.1 mg/l) at the end of the experimentation period fell within the FEPA and DPR limits of 10mg/l and 30mg/l for THC and BOD respectively.The results above thus indicates that the use of mixed microbial population of Pseudomonas aureginosa, Penicillium notatum, E. Coli and

scholarly journals Impacts of Artisanal Crude Oil Refining Activities on Soil Microorganisms

2019 ◽  
pp. 1-9
Author(s):  
Douglas, Salome Ibietela ◽  
Cornelius, Blessing Tamunonegiyeofori
Keyword(s):  
Crude Oil ◽  
Soil Microorganisms ◽  
Microbial Population ◽  
Soil Samples ◽  
Oil Refining ◽  
Bacterial Counts ◽  
Total Hydrocarbon ◽  
Hydrocarbon Content ◽  
Microbiological Methods ◽  
Total Hydrocarbon Content

Aim: To evaluate the effect of illegal crude oil refining activities on soil microorganisms using standard microbiological methods. Study Design: This study employs laboratory experimental design, statistical analysis of the data and interpretation. Place and Duration of Study:  Soil samples were taken once a month for three months (May- July, 2018) from Ke in Degema Local Government Area of Rivers State, Nigeria, where illegal crude oil refining activities are ongoing. Methodology: Using standard microbiological methods, total culturable heterotrophic bacterial counts, total fungal counts, Hydrocarbon utilizing bacterial and fungal counts were analysed to evaluate the effect of the activities. Total hydrocarbon content of the soil samples was also analysed. Results: The populations of the total heterotrophic bacterial, fungal and hydrocarbon utilizing bacterial (HUB) and fungal (HUF) counts of the contaminated soil were enumerated. The mean total heterotrophic bacterial counts in Station 1 around the pot ranged from 2.5 x 105 to 1.8 x 106cfu/g, fungal counts ranged from 2.1 x 103 to 4.4 x 104cfu/g, HUB (HUB) counts ranged from 4.2 x 104 to 6.4 x 105cfu/g and hydrocarbon utilizing fungal (HUF) counts ranged from 1.5 x 103 to 4.0 x 103cfu/g. The results of soil samples taken 20m away from the Pot location ranged from 7.0 x 105 to 8.2 x 106cfu/g for total heterotrophic bacterial counts, fungal counts ranged from 2.3 x 103 to 1.5 x 104cfu/g, HUB ranged from 4.7 x 104 to 5.7 x 105cfu/g and HUF ranged from 2.0 x 103 to 3.5 x 103cfu/g. Also, the results of total heterotrophic bacterial counts for Station 2 ranged from; 4.3 x 105 to 3.3 x 106cfu/g, fungi 2.0 x 103 to 3.3 x 104cfu/g, HUB ranged from 3.8 X 104 to 5.4 x 104cfu/g  and HUF 1.6 x 103 to 3.5 x 103cfu/g, while 20m away from the Pot total heterotrophic bacteria ranged from 1.3 x 107 to 6.5 x 107cfu/g, fungi 5.8 x 103 to 1.4 x 105cfu/g, HUB 5.4 x 104 to 1.1x 105cfu/g and HUF 3.1 x 103 to 4.7 x 104cfu/g. While the control samples taken from inside the community where no such activity is on, ranged from 2.6 x 107 to 7.9 x 107cfu/g for total heterotrophic bacterial counts, total heterotrophic fungal counts ranged from 2.8 x 104 to 5.3 x 104cfu/g, HUB 2.0 x 102 to 3.1 x 102cfu/g and HUF 2.0 x 101 to 2.3 x 101cfu/g. twelve bacterial genera were identified and eight fungal genera: Bacillus, Alcaligenes, Flavobacterium, Acinetobacter, Pseudomonas, Micrococcus, Proteus, Serratia, Enterobacter, Streptococcus, Escherichia, Staphylococcus, Penicillum, Aspergillus, Fusarium, Mucor, Rhizopus, Geotrichum, Candida, and Cladosporium. Total hydrocarbon content ranged from 106 to 281mg/kg across the locations.  When compared with the control, it was observed that the microbial population and diversity were adversely affected. These variations observed in the microbial population are indicative of the effect of the illegal refinery on the soil microorganisms. Conclusion: The results of this study indicates that the continuous contamination of the soil environment by the activities of illegal crude oil refining, lead to a decrease in microbial population and diversity. This may result in devastating ecological damage, adversely affecting the ecological balance which may affect food chain and in turn animals and humans.

scholarly journals Elephant Grass (Pennisetum Purpureum) Mediated Phytoremediation of Crude Oil-Contaminated Soil

2019 ◽  
Vol 3 (1) ◽  
pp. 105-111 ◽  
Author(s):  
L. O. Bobor ◽  
B. E. Omosefe
Keyword(s):  
Crude Oil ◽  
Contaminated Soils ◽  
Control Sample ◽  
Bacterial Count ◽  
Pennisetum Purpureum ◽  
Elephant Grass ◽  
Total Hydrocarbon ◽  
Hydrocarbon Content ◽  
And Control ◽  
Total Hydrocarbon Content

Phytoremediation is an economic and environmentally friendly method for the remediation of hazardous crude oil contaminated soils. In this study, phytoremediation of crude oil contaminated soils by elephant grass (pennisetum purpureum) was investigated over a 40-day period. Grass clumps were harvested and transplanted into plastic buckets filled with 1kg of soil contaminated with 30 ml and 40 ml of crude oil and an uncontaminated control sample. An additional control sample was contaminated with 30 ml of crude oil with no elephant grass. The samples were analyzed periodically for changes in pH, total hydrocarbon content, total viable bacterial count, and total fungal count. The pH of the soil samples generally ranged from 5.26 to 7.85. After 40 days of treatment, the total hydrocarbon content decreased from 320 mg/kg to 38 mg/kg and from 590 mg/kg to 46 mg/kg in samples contaminated with 30 ml and 40 ml of crude oil respectively. Plant growth was uninhibited in contaminated and control samples as the heights increased by 34.5-42.8 cm. The results of the study further demonstrate the phytoremediation capabilities and tolerance of elephant grass in crude-oil contaminated microcosms.

scholarly journals Bioremediation Potential of Pseudomonas aeruginosa KX828570 on Crude Oil Spill Polluted Marshland and Terrestrial Soil Treated with Oil Spill Dispersant

2019 ◽  
pp. 1-15
Author(s):  
Renner Renner Nrior ◽  
Augusta Ogechi Inweregbu
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Moisture Content ◽  
Crude Oil ◽  
Oil Spill ◽  
Heterotrophic Bacteria ◽  
Soil Samples ◽  
Total Hydrocarbon ◽  
Hydrocarbon Content ◽  
Rivers State ◽  
Total Hydrocarbon Content

Aim: To investigate bioremediation potentiality of Pseudomonas aeruginosa KX828570 on crude oil Polluted Marshland and Terrestrial Soil treated with oil spill dispersant Study Design: The study employs experimental design, statistical analysis of data and interpretation. Place and Duration of the Study: Soil samples were collected from K-Dere, Gokana L.G.A, and were transported to the Microbiology Laboratory of Rivers State University, Port Harcourt, Nigeria for analyses while Oil spill dispersant (OSD/LT and OSD/Seacare) were from Barker and Hughes Nig Ltd (formally mil park Nigeria limited), all in Rivers state, Nigeria. This investigation study lasted for 28 days and sampling was done every 7day period. Methodology: Eight experimental set up were carried out using Pseudomonas aeruginosa KX828570 as the bio-augmenting organism in terrestrial and marshland soil contaminated with two Oil spill dispersant (OSD/LT and OSD/Seacare) separately. Controls for the two soil types were made without organism and treatment. Its bioremediation potential on the pollutants and two types of soil were monitored for 28 days. The setup was tilled twice a week to provide moisture and more oxygen for the organisms to thrive. Analysis of pH, Temperature, Moisture content, Total Hydrocarbon Content, Total Heterotrophic Bacteria, Dispersant Utilizing Bacteria was carried out at weekly intervals. Results: The pH of both soils ranged from 5.75 to 7.37; temperature from 270C to 340C; moisture content 03 to 0.6 across the soil samples. Total Hydrocarbon Content (THC) showed a steady decline from day 1 – 28. The percentage (%) bioremediation rates of polluted soils were as follows: Terrestrial soil+OSD/Seacare+Pseudomonas aeruginosa KX828570 69.4% > Terrestrial soil+OSD/LT+ Pseudomonas aeruginosa KX828570 65.9% > Marshland soil+OSD/Seacare+ Pseudomonas aeruginosa KX828570 52.6% > Marshland soil+OSD/Seacare+ Pseudomonas aeruginosa KX828570 47.6%. Dispersant utilizing bacterial count in marshland and terrestrial soil ranged from 6.013 to 7.338 log10Cfu/g and 6.045 to 7.301 log10Cfu/g respectively from Day 1 to 28. Conclusion:  From the investigation, it revealed that Oil spill dispersants are more degradable in terrestrial soil than marshland soil. OSD/Seacare is more biodegradable than OSD/LT in both terrestrial and marshland soil augmented with Pseudomonas aeruginosa KX828570. Thus, Pseudomonas aeruginosa KX828570 have been found to be a potential bioremediation agent in oil spill dispersant polluted marshland and terrestrial soil.

scholarly journals Impact of Illegal Crude Oil Refining in Jike-ama River of Bille Kingdom, Rivers State, Nigeria

2020 ◽  
pp. 46-51
Author(s):  
Nwachoko, Ndidi ◽  
Davies, Bekinbo ◽  
Tetam, Jack Gbenenee
Keyword(s):  
Heavy Metals ◽  
Crude Oil ◽  
Metal Content ◽  
Oil Refining ◽  
Permissible Limits ◽  
Water And Sediment ◽  
Hydrocarbon Content ◽  
Significant Difference ◽  
The Impact ◽  
Total Hydrocarbon Content

Oil exploration and exploitation is a lucrative business and one of the major sources of revenue in Nigeria. Each year, hundreds of post-impact assessment (PIA) studies are conducted to assess the impact of the hazards generated by the oil industry on social environment and on human health. This hazardous impact is the associated with the activities of illegal refining of crude oil. Thus, this work examined the impact of illegal crude oil refining activities in Jike-Ama river of Jike-Ama community. Shellfish, fish, surface water and sediment samples were randomly collected in triplicates. Physiochemical parameters, heavy metal content, polycyclic aromatic hydrocarbons (PAHs) and total hydrocarbon content of the water in addition to heavy metal content and PAHs in fish, Shellfish and sediment were determined. The results of the physicochemical parameters showed that pH, total suspended solids, biological oxygen demand, turbidity and total hydrocarbon content of water were above FEPA permissible limits while chloride, nitrate and sulphate detected in water were within FEPA permissible limits. Dissolved oxygen was observed to be below FEPA permissible limit. The mean concentration of heavy metals and PAHs in sediment showed significant difference (p<0.05) when compared with values for Shellfish and fish and were above FEPA and WHO permissible limits. Shellfish had higher concentrations of heavy metals and PAHs than fish. PAHs values in shellfish showed significant difference (p<0.05). Heavy metals concentration in fish were above permissible limits except Cr, Zn and Pb. Concentration of PAHs in fish, shellfish, surface water and sediment were generally above FEPA and WHO permissible limits. This findings suggests high concentration of contaminants in Jike-Ama river.

scholarly journals Stimulating the Rate of Phytoremediation of Chromolaena odorata in Crude Oil Contaminated Soil Using Inorganic Urea

2020 ◽  
pp. 13-25
Author(s):  
F. B. G. Tanee ◽  
K. Jude
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Latin Square ◽  
Polluted Soil ◽  
Total Petroleum Hydrocarbon ◽  
Test Plant ◽  
Chromolaena Odorata ◽  
Hydrocarbon Content ◽  
Total Hydrocarbon Content

Investigation on the use of urea in stimulating the phytoremediation of Chromolaena odorata in a crude oil contaminated soil was carried out at a crude oil spilled site at Botem-Tai, Ogoni, Nigeria. Three phytoremediation treatments labeled A – C in addition to the control (D) were used. The treatments were: A (Chromolaena odorata only), B (Chromolaena odorata + 20 g/m2 urea), C (Chromolaena odorata + 40 g/m2 urea), D (polluted soil without phytoremediation) arranged using Latin Square Design (LSD). Total petroleum hydrocarbon (TPH) and Total hydrocarbon content (THC) in soil and plant samples from the different treatment plots in addition to other soil nutrients were analyzed. The percentage reduction in TPH and THC in soil were as follows: Treatment B, {TPH (92.08%) and THC (95.37%)} > treatment A {TPH (88.95%) and THC (93.37%)}> C {TPH (78.78%) and THC (83.29%)} > Control {TPH (14.76%) and THC (32.90%)}. Treatment C had the highest TPH (2.67 mg/kg) and THC (20.57 mg/kg) accumulation in test   plant. Combining stimulant (urea) with phytoremediation also improved soil properties such  as pH, Nitrogen and Potassium. With the highest reduction of TPH and THC in treatment B (phytoremediation with 20 g/m2 urea) than other treatments is an indication that low   concentration of urea has a stimulatory effect on phytoremediation of crude oil by Chromolaena odorata.

scholarly journals Evaluation of raw pork as a commercially manufactured diet option for zoo-managed African wildcats (Felis silvestris lybica)

2017 ◽  
Vol 1 (3) ◽  
pp. 397-405
Author(s):  
C. J. Iske ◽  
C. L. Morris ◽  
K. L. Kappen
Keyword(s):  
Microbial Population ◽  
Population Variation ◽  
Microbial Populations ◽  
Plate Count ◽  
Major Protein ◽  
Raw Meat ◽  
E Coli ◽  
The Us ◽  
Aerobic Plate Count ◽  
Dietary Treatments

Abstract Second to beef, pork is a major protein source produced in the US. Properly sourced and handled pork could be utilized as a protein option for zoo-managed carnivores. Concerns of high levels of microbial populations in raw meat diets are common. The objectives of this study were to determine apparent total tract macronutrient and energy digestibility and fecal scores from cats fed a commercially manufactured raw pork-based diet compared with commercially available raw carnivore diets formulated with either horse or beef and evaluate typical microbial population variation among the diets. Dietary treatments consisted of 4 raw meat-based diets: Horse, Beef, Pork, and beef/horse Blend. All diets were highly digestible, especially fat digestibility (98.6 to 99.7%) in which there were no statistical differences among diets. Digestibility of organic matter (OM) was greater (P = 0.05) when cats consumed the Blend diet (97.2%) compared to the Pork diet (93.1%). Fecal scores ranged from 1.6 to 2.6 (on a 5-point scale), with Beef (2.6) being greater than (P = 0.01) Horse (1.6) and (P = 0.02) Pork (1.9). E. coli counts ranged from 110 to 10,000 cfu/g; total coliforms: 150 to 28,000 cfu/g; yeast: 20 to 4,000 cfu/g; mold count: not detectable to 10 cfu/g; and aerobic plate count: 23,000 to 26,000,000 cfu/g. Staphylococcus aureus was not detected in any of the diets. Salmonella was presumptive positive in the Pork and Blend diet, and was negative in the other 2 diets. In conclusion, commercially manufactured diets have varying microbial counts. All diets, including the raw pork-based diet were well utilized by exotic small cats and can be included among dietary options for managed felids.

Sequential enrichment of microbial populations exhibiting enhanced biodegradation of crude oil

1995 ◽  
Vol 41 (9) ◽  
pp. 767-775 ◽  
Author(s):  
Kasthuri Venkateswaran ◽  
Shigeaki Harayama
Keyword(s):  
Crude Oil ◽  
Microbial Population ◽  
Saturated Hydrocarbon ◽  
Microbial Populations ◽  
Microbial Culture ◽  
Initial Screening ◽  
Oil Biodegradation ◽  
Degrading Bacteria ◽  
Enhanced Biodegradation ◽  
Enriched Cultures

The distribution of oil-degrading bacteria in the coastal water and sediments of Hokkaido, Japan, was surveyed. The potential of mixed microbial populations to degrade weathered crude oil was not confined to any ecological components (water or sediment) nor to the sampling stations. One microbial culture that was stable during repeated subculturing degraded 45% of the saturates and 20% of the aromatics present in crude oil in 10 days during the initial screening. The residual hydrocarbons in this culture were extracted by chloroform and dispersed in a fresh seawater-based medium and subsequently inoculated with microorganisms from the first culture. After full growth of the second culture, the residual hydrocarbons were again extracted and dispersed in a fresh medium in which microorganisms from the second culture had been inoculated. This sequential process was carried out six times to enrich those microorganisms that grew on the recalcitrant components of crude oil. After repeated exposure of the residual crude oil to the enriched microorganisms, about 80% of the initially added crude oil was degraded. The cultures obtained after each enrichment cycle were kept, and the degradation of fresh crude oil by the enriched microorganisms was examined. The degradative activity of the enriched cultures increased as the number of enrichment cycles increased. A microbial population that had been selected six times on the residual crude oil could degrade 70% of the saturates and 30% of the aromatics of crude oil. Thus, growth of a microbial population on residual crude oil improved its ability to biodegrade crude oil.Key words: crude oil, biodegradation, sequential enrichment, saturated hydrocarbon, aromatic hydrocarbon.

Synthesis of Chalcones and Nucleosides Incorporating [1, 3, 4]Oxadiazolenone Core and Evaluation of their Antifungal and Antibacterial Activities

2020 ◽  
Vol 15 (6) ◽  
pp. 665-679
Author(s):  
Alok K. Srivastava ◽  
Lokesh K. Pandey
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Good Antibacterial Activity

Background: [1, 3, 4]oxadiazolenone core containing chalcones and nucleosides were synthesized by Claisen-Schmidt condensation of a variety of benzaldehyde derivatives, obtained from oxidation of substituted 5-(3/6 substituted-4-Methylphenyl)-1, 3, 4-oxadiazole-2(3H)-one and various substituted acetophenone. The resultant chalcones were coupled with penta-O-acetylglucopyranose followed by deacetylation to get [1, 3, 4] oxadiazolenone core containing chalcones and nucleosides. Various analytical techniques viz IR, NMR, LC-MS and elemental analysis were used to confirm the structure of the synthesised compounds.The compounds were targeted against Bacillus subtilis, Staphylococcus aureus and Escherichia coli for antibacterial activity and Aspergillus flavus, Aspergillus niger and Fusarium oxysporum for antifungal activity. Methods: A mixture of Acid hydrazides (3.0 mmol) and N, Nʹ- carbonyl diimidazole (3.3 mmol) in 15 mL of dioxane was refluxed to afford substituted [1, 3, 4]-oxadiazole-2(3H)-one. The resulted [1, 3, 4]- oxadiazole-2(3H)-one (1.42 mmol) was oxidized with Chromyl chloride (1.5 mL) in 20 mL of carbon tetra chloride and condensed with acetophenones (1.42 mmol) to get chalcones 4. The equimolar ratio of obtained chalcones 4 and β -D-1,2,3,4,6- penta-O-acetylglucopyranose in presence of iodine was refluxed to get nucleosides 5. The [1, 3, 4] oxadiazolenone core containing chalcones 4 and nucleosides 5 were tested to determined minimum inhibitory concentration (MIC) value with the experimental procedure of Benson using disc-diffusion method. All compounds were tested at concentration of 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL, 0.62 mg/mL, 0.31 mg/mL and 0.15 mg/mL for antifungal activity against three strains of pathogenic fungi Aspergillus flavus (A. flavus), Aspergillus niger (A. niger) and Fusarium oxysporum (F. oxysporum) and for antibacterial activity against Gram-negative bacterium: Escherichia coli (E. coli), and two Gram-positive bacteria: Staphylococcus aureus (S. aureus) and Bacillus subtilis(B. subtilis). Result: The chalcones 4 and nucleosides 5 were screened for antibacterial activity against E. coli, S. aureus and B. subtilis whereas antifungal activity against A. flavus, A. niger and F. oxysporum. Compounds 4a-t showed good antibacterial activity whereas compounds 5a-t containing glucose moiety showed better activity against fungi. The glucose moiety of compounds 5 helps to enter into the cell wall of fungi and control the cell growth. Conclusion: Chalcones 4 and nucleosides 5 incorporating [1, 3, 4] oxadiazolenone core were synthesized and characterized by various spectral techniques and elemental analysis. These compounds were evaluated for their antifungal activity against three fungi; viz. A. flavus, A. niger and F. oxysporum. In addition to this, synthesized compounds were evaluated for their antibacterial activity against gram negative bacteria E. Coli and gram positive bacteria S. aureus, B. subtilis. Compounds 4a-t showed good antibacterial activity whereas 5a-t showed better activity against fungi.

scholarly journals Enhancement of the soil quality of an oil-polluted ultisol using livestock wastes

2020 ◽  
Vol 12 (2) ◽  
pp. 387-398
Author(s):  
Sylvia O. OGOANAH ◽  
Uzoamaka N. NGWOKE ◽  
Edokpolor O. OHANMU ◽  
Pascal C. OKOYE ◽  
Beckley IKHAJIAGBE
Keyword(s):  
Soil Quality ◽  
Polluted Soil ◽  
Lubricating Oil ◽  
Panicum Maximum ◽  
Potential Candidate ◽  
Bacterial Colony ◽  
Total Hydrocarbon ◽  
Hydrocarbon Content ◽  
Total Hydrocarbon Content

The study investigated the enhancement of soil quality of an oil-polluted ultisol using livestock wastes. Top soil (0 - 10 cm) was obtained as a pooled sample and polluted with spent lubricating oil at 10% w/w. The soil was subsequently amended with sun-dried goat (GT), rabbit (RB), and poultry (PG) dung at 10% w/w on dry weight basis both in singles, double-mixed, and triple-mixed combinations. Twelve weeks after treatment application, results showed that there was a 93.9% decrease (p<0.05) in bacterial colony count in the oil-polluted soil compared to the control. Penicillium notatum and Aspergillus niger as well as Bacillus sp. and Proteus sp. were the prominent fungal and bacterial species identified respectively. The most abundant plant in the soil seed bank was Panicum maximum with 10.4% abundance and this showed possible involvement of the plant in remediation of oil-pollution. The total hydrocarbon content of the oil-polluted soil was 9984.0 mg/kg, compared to 3170.6 mg/kg when amended with RB+GT, implying 76.77% remediation efficiency. Among several trials employed in this study, the combination of rabbit and goat wastes proved to be more effective in reducing the total hydrocarbon content of oil-polluted soil and therefore, is recommended as a potential candidate for application in the bioremediation of such soil.

Sign in / Sign up

Export Citation Format

Share Document