Cell Proliferation on Titania Layer with In Vitro Apatite Forming Ability

2007 ◽  
Vol 330-332 ◽  
pp. 131-134
Author(s):  
S. Yabe ◽  
Kanji Tsuru ◽  
Satoshi Hayakawa ◽  
Akiyoshi Osaka ◽  
Y. Yoshida ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Hydrogen Peroxide ◽  
Cell Proliferation ◽  
Body Fluid ◽  
Surface Hydrophobicity ◽  
Diffraction Intensity ◽  
Titania Layer ◽  
Xrd Patterns ◽  
Titanium Substrates

Titania layer was fabricated on the titanium substrates with chemical treatment with 20ml or 40ml of hydrogen peroxide solution and subsequent heat treatment at 400°C, coded as CHT20 and CHT40, respectively. CHT20 spontaneously deposited apatite on the surface in a simulated body fluid (SBF), while CHT40 did not. TF-XRD patterns showed that the diffraction intensity of anatase of CHT20 was higher than that of CHT40. It was suggested that the thicker titania layer indicated in vitro apatite forming ability. The cell proliferation of CHT20 and CHT40 were lower than NT and HT. Since the surface of titania layers became hydrophobic after autoclaving, we can suppose that the cell proliferation on CHT20 and CHT40 were lower than NT and HT due to their surface hydrophobicity.

Improvement of Apatite-Forming Ability of Tantalum Metal by CaCl2 Treatment

2007 ◽  
Vol 361-363 ◽  
pp. 681-684
Author(s):  
Deepak K. Pattanayak ◽  
Tomiharu Matsushita ◽  
Hiroaki Takadama ◽  
Tadashi Kokubo ◽  
Takashi Nakamura
Keyword(s):  
Heat Treatment ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Edx Analysis ◽  
Na Ions ◽  
Naoh Treatment ◽  
Tantalum Metal ◽  
Cacl2 Treatment ◽  
Xrd Patterns

Tantalum metal was soaked in NaOH and CaCl2 solutions, and then subjected to heat treatment at 500°C. EDX analysis showed that about 6.5 at. % of Na was incorporated into the surface of the tantalum metal by the first NaOH treatment. These Na+ ions were replaced by Ca2+ ions by the subsequent CaCl2 treatment. According to TF-XRD patterns, an amorphous sodium tantalate was seemed to be formed on the tantalum metal by the NaOH treatment and transformed into amorphous calcium tantalate by the CaCl2 treatment. This phase was crystallized into Ca2Ta2O7 by heat treatment. Critical detaching load of the surface of the CaCl2-treated tantalum metal was as low as 5mN, while as high as 42mN after the heat treatment. Apatite-forming ability of the NaOH-treated tantalum metal in a simulated body fluid (SBF) was appreciably increased by the CaCl2 treatment and maintained even after the heat treatment.

Effect of Annealing on Microstructure of Hydroxyapatite Coatings and their Behaviours in Simulated Body Fluid

2014 ◽  
Vol 922 ◽  
pp. 657-662 ◽  
Author(s):  
Sharidah Azuar Abdul Azis ◽  
John Kennedy ◽  
Peng Cao
Keyword(s):  
Heat Treatment ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Ion Beam ◽  
Apatite Formation ◽  
Ion Beam Sputtering ◽  
Different Temperatures ◽  
Higher Temperature ◽  
Xrd Patterns ◽  
Post Deposition

In this study, hydroxyapatite (HA) coatings on Ti6Al4V substrate were deposited using an ion beam sputtering technique. Owing to its medical applications, the crystalline phases present in the HA must be controlled. This study investigated the effect of post-deposition heat treatment at different temperatures and evaluated the microstructure of the HA coatings and their behaviours in simulated body fluid (SBF). The post-deposition treatment of the as-deposited samples was carried out in an air-circulated furnace at a temperature between 3000C and 6000C. The XRD patterns reveal that the minimum temperature to transform the HA coating from amorphous to crystalline phase is 4000C. A higher temperature at 6000C leads to a growth of the crystalline HA phases. Fourier transform infrared spectroscopy (FTIR) measurements show the existence of hydroxyl and PO-bonds in all coatings and the amounts varied with temperature. Atomic Force Microscopy (AFM) study suggests that the nanostructured crystalline HA starts to grow at 4000C and becomes more obvious at a higher temperature of 6000C. The simulated body fluid (SBF) test reveals that better apatite formation with post deposition heat treatment at 6000C would potentially enhance the formation of new bone (osseointegration).

Bioactivity of Plasma-Sprayed Diopside Coating In Vitro

2005 ◽  
Vol 288-289 ◽  
pp. 319-322 ◽  
Author(s):  
Wei Chang Xue ◽  
Chuan Xian Ding ◽  
Cong Cao ◽  
Yuqi Dong
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Proliferation ◽  
Cross Section ◽  
Body Fluid ◽  
Human Osteoblast ◽  
Suitable Candidate ◽  
Plasma Sprayed ◽  
Scanning Electron

A new bioceramic coating based on diopside was prepared by plasma spraying. The surface and cross-section microstructure of the coating were examined by scanning electron microscopy. The thermal expansion coefficient of the diopside coating measured by a dilatometer adapted to that of titanium alloy. The bond strength of the coating was about 32.5 MPa, which is higher than that of HA coatings used in orthopedics and dentistry. The bioactivity of diopside coating was evaluated in vitro. After 15 days soaking in simulated body fluid, an apatite layer was formed on the surface of the coating. The cytocompatibility was investigated by studying the behaviour of human osteoblast cultured directly onto the surface of the coating. MTT assay was performed to assess the influence of the coating on cell proliferation. The morphologies of the cell were observed by SEM after incubation for 1 and 7 days. The results obtained indicated that plasma sprayed diopside coating may be a suitable candidate for bone and dental implant.

In Vitro Characterization of 3D Beta-Tricalcium Phosphate Scaffolds Reinforced with Phosphate Based-Bioactive Glass for Bone Replacement

2016 ◽  
Vol 720 ◽  
pp. 108-113
Author(s):  
Criseida Ruiz-Aguilar ◽  
E.A. Aguilar-Reyes ◽  
Ana Edith Higareda-Mendoza ◽  
C.A. León-Patiño
Keyword(s):  
Heat Treatment ◽  
Tissue Engineering ◽  
Cell Proliferation ◽  
Bone Tissue ◽  
Present Contribution ◽  
Beta Tricalcium Phosphate ◽  
Bone Replacement ◽  
Factor Therapy

Bone tissue engineering is an excellent alternative to reduce bone disorders and conditions, by inducing new functional bone regeneration starting from the synthesis of the biomaterials and then the combination of cell and factor therapy. In the present contribution, the scaffolds were made with a ratio of 80 wt.% of β-TCP and 20 wt. % of phosphate-based bioglass, in addition the phosphate-based bioglass was reinforced with zirconia in different amounts (0, 0.5 and 1.0 mol%) with the aim to reduce the dissolution rate, improve the osteoconduction and the osteogenesis of the bone tissue. The results obtained by μCT of the scaffolds containing zirconia showed a wide pore size distribution between 1.5 and 303 μm. AlamarBlue assays showed that the cell proliferation of MC3T3-E1 preosteoblasts scaffolds were sixfold increase in relation to the number of the initial cells. FE-SEM helped to observe the cauliflower structure of HA and DRX showed that crystalline phases formed after heat treatment were (NaCaPO4 and NaZr5PO4) owing both to the crystallization and combination of the bioglass and β-TCP .

Heat Treatment Effect on Biological Behavior of Polyetheretherketone Composites

2022 ◽  
Vol 54 ◽  
pp. 119-128
Author(s):  
Alaa A. Mohammed ◽  
Jawad K. Oleiwi
Keyword(s):  
Heat Treatment ◽  
Simulated Body Fluid ◽  
Mtt Assay ◽  
Body Fluid ◽  
Antibacterial Properties ◽  
Biological Behavior ◽  
Apatite Formation ◽  
Crystalline Polymers ◽  
Density Methods

Polyetheretherketone is a semi-crystalline thermoplastic polymer, that so with heat treatments, it is possible to get different properties which are very important for the material performance. Heat treatment is a broadly utilized to develop the semi-crystalline polymers properties. In the present investigation, annealing of polyetheretherketone (PEEK) was carried out at temperatures above its glass transition temperature (Tg) to study its effects upon the biological conduct of the control and PEEK ternary composites. The bioactivity of the specimens was evaluated by investigating the apatite formation after immersion for different periods in a simulated body fluid (SBF). The biocompatibility of specimens was assessed by MTT assay. Additionally, the antibacterial property of the specimens versus S. aureus was observed with the optical density methods. The results manifested that the formation of hydroxyapatite was obviously observed on specimens after immersion for (7 and 14 days) in the simulated body fluid (SBF). Otherwise, the results of MTT assay recorded the PEEK specimens that excited the activity of fibroblasts, and therefore a high cytocompatibility was noticed and the specimens revealed antibacterial properties against S. aureus. So, the results of the bioactivity, biocompatibility and antibacterial tests in vitro demonstrated that the heat treatment enhanced biological behavior.

Evaluation of Sodium Titanate Coating on Titanium by Sol-Gel Method In Vitro

2007 ◽  
Vol 330-332 ◽  
pp. 777-780 ◽  
Author(s):  
Fang Fang Wang ◽  
Yun Mao Liao ◽  
Min Wang ◽  
Ping Gong ◽  
Xiao Yu Li ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Sol Gel ◽  
Sodium Titanate ◽  
Homogeneous Surface ◽  
Gel Method ◽  
Sol Gel Method ◽  
Xrd Patterns

In this study, an exclusive sodium titanate (Na2Ti6O13) coating on titanium was fabricated by sol-gel method and evaluated in vitro. The coating was characterized by SEM and XRD. The bioactivity of the Na2Ti6O13 coating was evaluated by the biomimetic growth of apatite on its surface after soaked in an acellular simulated body fluid (SBF) for a period of time. In vitro osteoblasts culture was carried out to determine cytocompatibility by the measurement of the proliferation and alkaline phosphatase (ALP) activity of the cells. XRD patterns showed that Na2Ti6O13 was well crystallized when the coating was heated at 800°C. SEM observation exhibited that the Na2Ti6O13 coated titanium had a homogeneous surface without any cracks. After immersion in SBF, the apatite layer can be formed on the coating. The cells culture showed that the osteoblasts grew well on the Na2Ti6O13 coated titanium. It can be concluded that Na2Ti6O13 coating on titanium obtained by sol-gel method is bioactive.

Preparation of Poly(L-lactic Acid) Hybrid Membrane with Silicon-Ion-Releasing Ability

2007 ◽  
Vol 330-332 ◽  
pp. 1305-1308 ◽  
Author(s):  
Hirotaka Maeda ◽  
Emile Hideki Ishida ◽  
Toshihiro Kasuga
Keyword(s):  
Heat Treatment ◽  
Cell Proliferation ◽  
Lactic Acid ◽  
Body Fluid ◽  
Cellular Proliferation ◽  
Hybrid Membrane ◽  
Poly Lactic Acid ◽  
Calcium Carbonates ◽  
Before And After ◽  
Proliferation Ability

A novel poly(lactic acid) (PLA)/calcium carbonates hybrid membrane containing siloxane was prepared using aminopropyltriethoxysilane (APTES) for biodegradable bone guided regeneration. The PLLA in the membrane was an amorphous phase. By heating the membrane at 100 °C for 1 h, the PLLA in the membrane crystallized. Numerous pores of 0.5-1 ,m in diameter were newly formed at the surface. After soaking the membranes before and after heat-treatment in simulated body fluid, the amount of silicon species in SBF released from the membrane after heat-treatment was higher than that before heat-treatment. A test of osteoblast-like cellular proliferation on the membrane showed the membrane after heat-treatment has much higher cell-proliferation ability than that before heat-treatment.

Dental Ceramics Modified by Ternary Glass-Ceramic Coatings: Characterization and In Vitro Bioactivity Study

2008 ◽  
Vol 396-398 ◽  
pp. 111-114
Author(s):  
Xanthippi Chatzistavrou ◽  
Nikolaos Kantiranis ◽  
Lambrini Papadopoulou ◽  
Eleana Kontonasaki ◽  
Aldo Roberto Boccaccini ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Ternary Mixture ◽  
Body Fluid ◽  
Ceramic Coatings ◽  
Dental Ceramics ◽  
Dental Ceramic ◽  
Crystal Phases ◽  
Glass Ceramic Coatings ◽  
Ceramic Hydroxyapatite

In this study new ternary bioactive mixtures were investigated, which are appropriate for applications as coatings on the surface of dental ceramics. The fabrication of mixtures based on the combination of dental ceramic, hydroxyapatite and bioactive glass was demonstrated. The mixtures were characterized by FTIR, XRD and SEM and their bioactive behavior was investigated by immersion in Simulated Body Fluid (SBF). The ternary mixture consisted of a high fusing leucite-feldspathic dental ceramic which exhibited the highest bioactive behavior assigned to the characteristic crystal phases occurring under the specific heat treatment investigated.

Cell Proliferation on Titania Layer with In Vitro Apatite Forming Ability

2007 ◽  
pp. 131-134
Author(s):  
S. Yabe ◽  
Kanji Tsuru ◽  
Satoshi Hayakawa ◽  
Akiyoshi Osaka ◽  
Y. Yoshida ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Titania Layer

scholarly journals Surface Modification of the Ti-6Al-4V Alloy by Anodic Oxidation and Its Effect on Osteoarticular Cell Proliferation

Coatings ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 491
Author(s):  
Itzel P. Torres-Avila ◽  
Itzia I. Padilla-Martínez ◽  
Nury Pérez-Hernández ◽  
Angel E. Bañuelos-Hernández ◽  
Julio C. Velázquez ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Titanium Oxide ◽  
Anodic Oxidation ◽  
Ammonium Fluoride ◽  
Biological Evaluation ◽  
In Vitro Assays ◽  
X Ray Diffraction ◽  
Inner Diameter ◽  
Xrd Patterns

This investigation describes the formation of crystalline nanotubes of titanium oxide on the surface of a Ti-6Al-4V alloy and its biological evaluation. The formation of nanotubes was performed by the anodic oxidation technique with a constant work potential of 60 V but with different anodizing times of 10, 20, 30, 40, 50, and 60 min used to evaluate their effects on the characteristics of the nanotubes and their biological activity. A mixture of ethylene glycol, water, and ammonium fluoride (NH4F) was used as the electrolytic fluid. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) were applied to determine the morphology and crystalline nature of the nanotubes, showing a well-defined matrix of nanotubes of titanium oxide with a crystalline structure and a diameter in the range of 52.5 ± 5.13 to 95 ± 11.92 nm. In contrast, the XRD patterns showed an increase of defined peaks that directly correlated with treatment times. Moreover, in vitro assays using an innovative cell culture device demonstrated that the inner diameter of the nanotubes directly correlated with cell proliferation.

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