Effects of Processing Parameters on the Morphology and Size of Electrospun PHBV Micro- and Nano-Fibers

2007 ◽  
Vol 334-335 ◽  
pp. 1233-1236 ◽  
Author(s):  
Ho Wang Tong ◽  
Min Wang
Keyword(s):  
Tissue Engineering ◽  
Polymer Solution ◽  
Polymer Concentration ◽  
Solution Concentration ◽  
Processing Parameters ◽  
Electrospun Fibers ◽  
Effective Technique ◽  
Electrospinning Technique ◽  
Needle Diameter ◽  
Short Time

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was used to fabricate micro- and nano-fibrous, non-woven mats by electrospinning for potential tissue engineering applications. The morphology and size of electrospun fibers were assessed systematically by varying the processing parameters. It was found that the diameter of the fibers produced generally increased with electrospinning voltage, needle diameter for the polymer jet and polymer solution concentration. Beaded fibers were readily produced at low PHBV concentrations, whereas the needle was blocked within a very short time during electrospinning when the PHBV concentration was too high. At the polymer concentration of 7.5 % w/v, it was shown that beadless PHBV fibers could be generated continuously by adjusting the electrospinning parameters to appropriate values. This study has clearly demonstrated that electrospinning can be an effective technique to produce PHBV micro- and nano-fibers. It has also been shown that composite fibers containing hydroxyapatite (HA) can be produced using the electrospinning technique.

scholarly journals Processing parameters for electrospinning poly(methyl methacrylate) (PMMA)/titanium isopropoxide composite in a pump-free setup

2018 ◽  
Vol 1 ◽  
pp. 27
Author(s):  
Leah Nyangasi ◽  
Dickson Andala ◽  
Charles Onindo ◽  
Alphonse Wanyonyi ◽  
Josphine Chepngetich
Keyword(s):  
Methyl Methacrylate ◽  
Polymer Solution ◽  
Solution Concentration ◽  
Titanium Isopropoxide ◽  
Processing Parameters ◽  
Solution Viscosity ◽  
Poly Methyl Methacrylate ◽  
Size And Morphology ◽  
Set Up ◽  
Almost All

Background: Electrospinning is a technique for producing nanofibers, useful in many fields of nanotechnology. The size and morphology of the nanofibers obtained depends on the polymer solution properties, the parameters of the equipment and the conditions of the surrounding. In almost all reported electrospinning set ups, a pump ,which regulates the flow of the polymer solution, has been included as one of the requirements. In this study, the effects of solution concentration, viscosity, voltage and the distance from the tip of the syringe to the aluminum collector on the morphology and diameters of poly(methyl methacrylate)(PMMA) fibers were investigated, using a pump-free electrospinning set up. Methods: Varied PMMA concentration (50 -120 mg/mL), voltage (10-18 kV) and distance (5 – 18 cm) of electrospinning were studied and the optimum electrospinning conditions identified.  PMMA/ titanium isopropoxide solution of ratio 1:2 was prepared, electrospun at optimized conditions (15 kV, 18 cm, Dichloromethane/Dimethylformamide 60:40) and the fibers obtained analyzed using a scanning electron microscope. Results: Solutions of PMMA whose concentrations were less than 50 mg/mL, produced beads on fibers, whereas those at ~ 100 mg/mL formed the best bead-free fibers of diameter 350±50 nm. The results showed a direct dependence of fiber diameter on the solution viscosity. Fibers of larger diameters were obtained when the distance from the tip of the syringe to the aluminum collector and voltage were increased but at higher distances (>18 kV) fewer fibers were collected. When the voltage was steadily increased, the fibers broadened and the diameters were non-uniform due to splaying and splitting. Increasing the distance between the pipette-tip and the collector from 10 to 18 cm resulted in reduced electric field which in turn yielded fewer fibers. Conclusions: The results obtained in a pump free set-up were comparable to those eletrospun in the presence of a pump.

Effects of Process Parameters on Formation of Hybrid Tissue Constructs

2013 ◽  
Author(s):  
Karen Chang Yan ◽  
Pamela Hitscherich ◽  
James Ferrie
Keyword(s):  
Tissue Engineering ◽  
Processing Parameters ◽  
Living Cells ◽  
Live Cells ◽  
Solution Flow Rate ◽  
Electrospinning Technique ◽  
Solution Flow ◽  
Tissue Constructs ◽  
Key Issues ◽  
Working Distance

Tissue engineering is a promising aspect of regenerative medicine that is aimed at constructing functional tissues and organs. While progresses in tissue engineering have led successful clinic applications, challenges remain for more complex tissues/organs that require concerted efforts from multiple types of cells. One of the key issues in building replacements for complex tissues/organs is to mimic the organ’s complex natural organization using a mixture of engineered materials and living cells [1]. Electrospinning has shown promise as a technique to create the microenvironment necessary for cell growth and proliferation for tissue engineering applications[2–4], while multiple fabrication methods have been developed to manipulate live cells(e.g. cell printing) [5–7]. To this end, a system integrating polymer electrospinning technique and pressure-driven cell deposition method is currently under development for forming hybrid tissue constructs with living cells and polymers. This study focuses on examining morphology of electrospun fibers as function of processing parameters including working distance and solution flow rate.

Polycaprolactone(PCL)/Gelati(Ge)-Based Electrospun Nanofibers for Tissue Engineering and Drug Delivery Application

2014 ◽  
Vol 554 ◽  
pp. 57-61 ◽  
Author(s):  
Lor Huai Chong ◽  
Mim Mim Lim ◽  
Naznin Sultana
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Electrospun Nanofibers ◽  
Processing Parameters ◽  
Natural Polymer ◽  
Electrospinning Technique ◽  
Minimum Concentration ◽  
Promising Alternative ◽  
Injured Tissue ◽  
Biomaterial Scaffold

Recent development of tissue engineering has been emphasized on tissue regeneration and repairing in order to solve the limitation of organ and tissue transplantation issues. Biomaterial scaffold, which plays an important role in this development, not only provides a promising alternative in order to improve the efficiency of cell transplantation in tissue engineering but also to deliver cells with growth factors and drugs into injured tissue to increase the survival of cell via drug delivery system. In this study, nanofibers were fabricated through blending of a synthetic polymer polycaprolactone (PCL) and a natural polymer Gelatin (Ge) using electrospinning technique. Processing parameters were optimized to determine the most suitable properties of PCL/Ge nanofibers. The surface morphology of PCL/Ge nanofibers were then characterized using Scanning Electron Microscopy (SEM). Six samples of nanofibers from different amount of gelatin mixed with 10% PCL (w/v) were successfully fabricated. Experimental results showed that 18kV of high voltage provided more homogenous and less beaded nanofibers. Meanwhile, the 0.8g of Ge in 10% PCL (w/v) was set as the maximum concentration while 0.2g of Ge in 10% PCL (w/v) was set as the minimum concentration to reduce the bead formation.

scholarly journals A new design of an electrospinning apparatus for tissue engineering applications

2017 ◽  
Vol 3 (2) ◽  
Author(s):  
Juliana R. Dias ◽  
Cyril Dos Santos ◽  
João Horta ◽  
Pedro Lopes Granja ◽  
Paulo Jorge Da Silva Bartolo
Keyword(s):  
Tissue Engineering ◽  
Simple Technique ◽  
Electrospun Nanofibers ◽  
Processing Parameters ◽  
Wound Dressings ◽  
Engineering Applications ◽  
Electrospinning Technique ◽  
Skin Tissue Engineering ◽  
Spinning Process ◽  
The Stability

The electrospinning technique is being widely explored in the biomedical field due to its simplicity to produce meshes and its capacity to mimic the micro-nanostructure of the natural extracellular matrix. For skin tissue engineering applications, wound dressings made from electrospun nanofibers present several advantages compared to conventional dressings, such as the promotion of the hemostasis phase, wound exudate absorption, semi-permeability, easy conformability to the wound, functional ability and no scar induction. Despite being a relatively simple technique, electrospinning is strongly influenced by polymer solution characteristics, processing parameters and environmental conditions, which strongly determine the production of fibers and their morphology. However, most electrospinning systems are wrongly designed, presenting a large number of conductive components that compromises the stability of the spinning process. This paper presents a new design of an electrospinning system solving the abovementioned limitations. The system was assessed through the production of polycaprolactone (PCL) and gelatin nanofibers.  Different solvents and processing parameters were considered. Results show that the proposed electrospinning system is suitable to produce reproducible and homogeneous electrospun fibers for tissue engineering applications.

Fabrication and Characterization of Polycaprolactone (PCL)/Gelatin Electrospun Fibers

2014 ◽  
Vol 554 ◽  
pp. 52-56 ◽  
Author(s):  
Mim Mim Lim ◽  
Naznin Sultana ◽  
Azli Bin Yahya
Keyword(s):  
Fiber Diameter ◽  
Electrospun Fiber ◽  
Weight Ratio ◽  
Processing Parameters ◽  
Electrospun Fibers ◽  
Average Fiber Diameter ◽  
Polymer Blending ◽  
Electrospinning Technique ◽  
Sem Image

Over the past few decades, there has been considerable interest in developing electrospun fibers by using electrospinning technique for various applications. Polymer blending is one of the most effective methods in providing desired properties. In this study, synthetic polymer polycaprolactone (PCL) was blended together with natural polymer gelatin where both of them have different properties. It is done by using electrospinning technique. 10 %w/v and 14 %w/v PCL/gelatin electrospun fibers were successfully electrospun with different weight ratio. Processing parameters were set constant in this study and only solution parameters were altered. The optimized electrospun fiber formed was 14 %w/v PCL/gelatin 70:30 with average fiber diameter of 246.30 nm. No beaded fiber was formed in this scanning electron microscope (SEM) image. The result obtained also showed that by increasing the overall polymeric concentration of PCL/gelatin, average fiber diameter decreases. Fiber diameter was also found decreasing with the increase of the concentration of gelatin in the same concentratoin of PCL/gelatin blended electrospun fiber. Blending of PCL and gelatin in different weight ratio had provided different properties of electrospun fibers. It is believed that blended electrospun fibers can be used for biomedical applications.

scholarly journals Sputtering of Electrospun Polymer-Based Nanofibers for Biomedical Applications: A Perspective

Nanomaterials ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 77 ◽  
Author(s):  
Hana Kadavil ◽  
Moustafa Zagho ◽  
Ahmed Elzatahry ◽  
Talal Altahtamouni
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Surface Modification ◽  
Wound Dressing ◽  
Biomedical Applications ◽  
Fiber Composites ◽  
Metal Particles ◽  
Electrospun Fibers ◽  
Electrospinning Technique ◽  
Wide Range

Electrospinning has gained wide attention recently in biomedical applications. Electrospun biocompatible scaffolds are well-known for biomedical applications such as drug delivery, wound dressing, and tissue engineering applications. In this review, the synthesis of polymer-based fiber composites using an electrospinning technique is discussed. Formerly, metal particles were then deposited on the surface of electrospun fibers using sputtering technology. Key nanometals for biomedical applications including silver and copper nanoparticles are discussed throughout this review. The formulated scaffolds were found to be suitable candidates for biomedical uses such as antibacterial coatings, surface modification for improving biocompatibility, and tissue engineering. This review briefly mentions the characteristics of the nanostructures while focusing on how nanostructures hold potential for a wide range of biomedical applications.

scholarly journals Research concerning fabrication of fibrous osteoconductive plga/hap nanocomposite material using the method of electrospinning from polymer solution

2013 ◽  
Vol 13 (3) ◽  
pp. 57-66
Author(s):  
Izabella Krucińska ◽  
Maciej Boguń ◽  
Olga Chrzanowska ◽  
Michał Chrzanowski ◽  
Paulina Król
Keyword(s):  
Dimethyl Sulfoxide ◽  
Polymer Solution ◽  
Rheological Properties ◽  
Biodegradable Polymer ◽  
Polymer Solutions ◽  
Solution Concentration ◽  
Commercial Product ◽  
Electrospinning Technique ◽  
Macroscopic Structure ◽  
Trade Name

Abstract The aim of the work was to obtain nano fibrous structures from biodegradable polymer with the addition of hydroxyapatite using electrospinning technique. Research was conducted with two types of solvent: dichloromethane and 50:50 mixture of dimethyl sulfoxide and dichloromethane. As a polymer a copolymer of L-lactide and glycolide (PLGA), commercial product with trade name Resomer®LG 824, was used. The preliminary electrospinning tests enabled to match optimal polymer solution concentration of tested samples. Rheological properties of all tested polymer solutions has been determined. Influence of electrospinning conditions and the type of solvent on macroscopic structure has been investigated.

Preparation of Drug Loaded Polyurethane Thin Layer on the Silicone Tube by Electrospinning Technique for Stent Application

2014 ◽  
Vol 804 ◽  
pp. 131-134
Author(s):  
Dong Geun Shin ◽  
Y.J. Lee ◽  
Y. Kim ◽  
W.T. Kwon ◽  
S.R. Kim ◽  
...  
Keyword(s):  
Thin Layer ◽  
Polymer Solution ◽  
Coating Thickness ◽  
Adhesive Strength ◽  
Atmospheric Pressure ◽  
Drug Loading ◽  
Atmospheric Pressure Plasma ◽  
Solution Concentration ◽  
Silicone Tube ◽  
Electrospinning Technique

Drug loaded polyurethane (PU) thin layer was prepared on the silicone tube by electrospinning technique. Microstructure of the PU layer was varied from nanoporous web to dense coating depending on the polymer solution concentration and the amount of drug loaded. It can be easily adjusted the coating thickness and porosity accurately and controlled the drug loading and releasing properties. However, adhesive strength between PU layer and silicone tube was very weak and easily broken away and white turbidity also another important problem. So, surface of silicone tube was atmospheric-pressure plasma (APP) treated for improving the adhesive and removing white turbidity phenomenon.

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