Evaluation of the Effects of Ag Ion Concentration on Osteoblast Activity

We reported the apatite-forming ability of 30CaO∙70SiO2 scaffolds with 0~100 ppm Ag ions by soaking in simulated body fluid (SBF). This study was to evaluate the effects of the concentrations of Ag ions in the 30CaO∙70SiO2 gels on in-vitro biocompatibility of osteoblasts (MC3T3). After seeding cells on the surface of Ag-30CaO∙70SiO2 gels scaffold, cellular behaviors were evaluated by an assay of cell counting kit-8. Cytotoxicity of the scaffold samples was evaluated by employing the extract solutions of the scaffold samples by the assays of neutral red, MTT and BrdU. In addition, live & dead assay was performed by using a gel covering method, which the scaffolds have been directly contacted with the incubated cells on the well plate. According to the results of CCK-8 assay, the optical density value of the absorbance of the resulting solution decreased as the concentration of Ag ions in the scaffolds increased. Moreover, their cell viability was measured to be less than 50% at the Ag concentrations of 50 ppm or more, and dead cells were observed in the experiment results of both the cytotoxicity and gel covering tests. From these experimental results, we concluded that the Ag-30CaO∙70SiO2 scaffolds with less than 50 ppm Ag ion concentration were considered as biocompatible.

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Nghiên cứu tính chất vật liệu thủy tinh y sinh 45S tổng hợp từ nguyên liệu chính cát trắng

VNU Journal of Science Natural Sciences and Technology ◽

10.25073/2588-1140/vnunst.4506 ◽

2017 ◽

Vol 33 (2) ◽

Author(s):

Bùi Xuân Vương

Keyword(s):

Simulated Body Fluid ◽

Cell Viability ◽

Bioactive Glass ◽

Body Fluid ◽

In Vitro Bioactivity ◽

Melting Method ◽

Good Cell ◽

Glass Powders

A bioactive glass 46S6 with composition 46% SiO2 - 24% CaO - 24% Na2O - 6% P2O5 (wt%) was elaborated by melting method. ‘‘In vitro’’ bioactivity of bio-glass was evaluated by soaking of glass-powders in a simulated body fluid (SBF) at different times. The obtained results highlighted the bioactivity of the bio-glass by the formation of a bioactive hydroxyapatite (HA) layer on its surface. Experiments ''in vitro'' in the presence of cells confirmed the non-toxicity and the good cell viability on this bio-glass.

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A Comparative Study Between Dynamic and Static Simulated Body Fluid Methods

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.271 ◽

2006 ◽

Vol 309-311 ◽

pp. 271-274 ◽

Cited By ~ 1

Author(s):

Ji Yong Chen ◽

You Rong Duan ◽

Chun Lin Deng ◽

Qi Yi Zhang ◽

Xing Dong Zhang

Keyword(s):

Simulated Body Fluid ◽

Body Fluid ◽

Good Method ◽

Nucleation And Growth ◽

Ion Concentration ◽

Osseous Tissue ◽

Key Factors ◽

Good Agreement

In vitro method has often been used in the biodegradation/bioactivity evaluation of bioactive ceramics for its convenience and saving in time and outlay. The simulated body fluid (SBF) suggested by Kokubo was a good simulation of the osteoproduction environment in osseous tissue and has been proved to be a good method to study the bioactivity of biomaterials and the mechanism of bone bonding. But SBF is not a suitable method to research the osteoinduction of biomaterials. The results from SBF were not consistent with that from in vivo in muscle. The local ion concentration is the key factors to affect the nucleation and growth of apatite. In muscle the effect of body fluid flowing on local ion concentration cannot be ignored. A dynamic SBF suggested by these authors of this paper not only simulated the ion concentration of body fluid, but also simulated the effect of body fluid flowing on the local ion concentration near the surface or in biomaterials in muscle. The results from the dynamic SBF were in good agreement with that of the implantation experiments in muscle. The results from dynamic SBF showed that apatite only formed on the walls of macropores of the porous CaP, no apatite formed on the surface of both dense and porous CaP. The new bone only formed on the walls of macropores of porous CaP implanted in muscles, no apatite or osseous tissue could be found on the surfaces of both porous and dense CaP. The dynamic SBF preferably simulated the osteoinduction environment in non-osseous tissue and can be used in osteoinductivity evaluation of bioceramics.

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Bioactivity Behavior of YSZ-Al2O3/10HAP Bioceramics Composites in Simulated Body Fluid

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.980.13 ◽

2014 ◽

Vol 980 ◽

pp. 13-17

Author(s):

M.R.N. Liyana ◽

Nur Maizatul Shima Adzali ◽

M.Z.M. Zamzuri

Keyword(s):

Simulated Body Fluid ◽

Body Fluid ◽

Immersion Time ◽

Ph Value ◽

Dental Materials ◽

Biological Response ◽

Ion Concentration ◽

Human Blood Plasma ◽

Apatite Formation

Yttria-stabilized zirconia and alumina made significant contributions to the development of health care industry, specifically as orthopedic and dental materials. Both bioceramics are nearly inert ceramics, as they do not allow the interfacial bonding with tissue. Thus, it is necessary to provide bioactive surrounding as to elicit a specific biological response at the interface of material. This research reported the microstructure and bioactivity behavior of YSZ-Al2O3/10HAP with 30 wt. % and 60 wt. % of YSZ content. Powders were mixed before being compacted at 225MPa using uni-axial press machine. The composites were sintered at 1200 ̊C with heating rate of 10 ̊C/min. In-vitro bioactivity behavior of the composites were evaluated by immersing the composites into simulated body fluid. Results from x-ray diffraction pattern, confirmed the phase formation of apatite by the presence of Ca2P2O7, and CaO that might be useful on implant cell interaction in a body environment. The apatite formation was observed on the surfaces of the composites by SEM only after 9 days of immersion and subsequently apatite nucleation increased with prolonging immersion time. The dynamic changes in pH, between ion concentration in SBF and bioceramics surfaces correspondedwith an immersion time. Up to 30 days of immersion, the pH value of SBF stabilized approximately around pH 7.4-7.6, similar to the human blood plasma. Formation of apatite on composites surface of prepared YSZ-Al2O3/10HAP bioceramics may contribute to the improved biocompatibility and osteoconductivity.

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IN-VITRO BEHAVIOUR OF BIPHASIC CALCIUM PHOSPHATE WITH DIFFERENT RATIO OF SILICA CONTENT IN SIMULATED BODY FLUID

Journal of industrial technology ◽

10.21908/jit.2015.8 ◽

2015 ◽

Vol 23 (1) ◽

pp. 1-14

Author(s):

Sudirman Sahid ◽

◽

Nor Shahida Kader Bashah ◽

Salina Sabudin ◽

◽

...

Keyword(s):

Calcium Phosphate ◽

Simulated Body Fluid ◽

Body Fluid ◽

Biphasic Calcium Phosphate ◽

Silica Content

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Tetracalcium Phosphate/Monetite/Calcium Sulfate Hemihdrate Biocement Powder Mixtures Prepared by the One-Step Synthesis for Preparation of Nanocrystalline Hydroxyapatite Biocement-Properties and In Vitro Evaluation

Materials ◽

10.3390/ma14092137 ◽

2021 ◽

Vol 14 (9) ◽

pp. 2137

Author(s):

Lubomir Medvecky ◽

Maria Giretova ◽

Radoslava Stulajterova ◽

Lenka Luptakova ◽

Tibor Sopcak

Keyword(s):

Simulated Body Fluid ◽

Body Fluid ◽

Calcium Sulfate ◽

Powder Mixtures ◽

Liquid Component ◽

Tetracalcium Phosphate ◽

Nanocrystalline Hydroxyapatite ◽

One Step ◽

Calcium Sulfate Hemihydrate

A modified one-step process was used to prepare tetracalcium phosphate/monetite/calcium sulfate hemihydrate powder cement mixtures (CAS). The procedure allowed the formation of monetite and calcium sulfate hemihydrate (CSH) in the form of nanoparticles. It was hypothesized that the presence of nanoCSH in small amounts enhances the in vitro bioactivity of CAS cement in relation to osteogenic gene markers in mesenchymal stem cells (MSCs). The CAS powder mixtures with 15 and 5 wt.% CSH were prepared by milling powder tetracalcium phosphate in an ethanolic solution of both orthophosphoric and sulfuric acids. The CAS cements had short setting times (around 5 min). The fast setting of the cement samples after the addition of the liquid component (water solution of NaH2PO4) was due to the partial formation of calcium sulfate dihydrate and hydroxyapatite before soaking in SBF with a small change in the original phase composition in cement powder samples after milling. Nanocrystalline hydroxyapatite biocement was produced by soaking of cement samples after setting in simulated body fluid (SBF). The fast release of calcium ions from CAS5 cement, as well as a small rise in the pH of SBF during soaking, were demonstrated. After soaking in SBF for 7 days, the final product of the cement transformation was nanocrystalline hydroxyapatite. The compressive strength of the cement samples (up to 30 MPa) after soaking in simulated body fluid (SBF) was comparable to that of bone. Real time polymerase chain reaction (RT-PCR) analysis revealed statistically significant higher gene expressions of alkaline phosphatase (ALP), osteonectin (ON) and osteopontin (OP) in cells cultured for 14 days in CAS5 extract compared to CSH-free cement. The addition of a small amount of nanoCSH (5 wt.%) to the tetracalcium phosphate (TTCP)/monetite cement mixture significantly promoted the over expression of osteogenic markers in MSCs. The prepared CAS powder mixture with its enhanced bioactivity can be used for bone defect treatment and has good potential for bone healing.

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Preparation and Degradation Characteristics of MAO/APS Composite Bio-Coating in Simulated Body Fluid

Coatings ◽

10.3390/coatings11060667 ◽

2021 ◽

Vol 11 (6) ◽

pp. 667

Author(s):

Zexin Wang ◽

Fei Ye ◽

Liangyu Chen ◽

Weigang Lv ◽

Zhengyi Zhang ◽

...

Keyword(s):

Simulated Body Fluid ◽

Composite Coating ◽

Body Fluid ◽

Composite Coatings ◽

Degradation Process ◽

Ceramic Coatings ◽

Immersion Test ◽

Substrate Material ◽

Atmospheric Plasma

In this work, ZK60 magnesium alloy was employed as a substrate material to produce ceramic coatings, containing Ca and P, by micro-arc oxidation (MAO). Atmospheric plasma spraying (APS) was used to prepare the hydroxyapatite layer (HA) on the MAO coating to obtain a composite coating for better biological activity. The coatings were examined by various means including an X-ray diffractometer, a scanning electron microscope and an energy spectrometer. Meanwhile, an electrochemical examination, immersion test and tensile test were used to evaluate the in vitro performance of the composite coatings. The results showed that the composite coating has a better corrosion resistance. In addition, this work proposed a degradation model of the composite coating in the simulated body fluid immersion test. This model explains the degradation process of the MAO/APS coating in SBF.

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Tổng hợp và nghiên cứu thực nghiệm "in vitro" vật liệu Hydroxyapatit trong môi trường giả dịch thể người SBF (Simulated Body Fluid)

Dong Thap University Journal of Science ◽

10.52714/dthu.14.8.2015.230 ◽

2015 ◽

pp. 87-91

Author(s):

Vuong Bui Xuan ◽

Vi Vo Thuy ◽

Hiep Dang Tan

Keyword(s):

Simulated Body Fluid ◽

Body Fluid

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In Vitro and In Vivo Degradation, Mechanical Properties, and Histocompatibility of Weft-Knitted Silk Mesh-Like Grafts

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2022.2911 ◽

2022 ◽

Vol 12 (2) ◽

pp. 411-416

Author(s):

Liang Tang ◽

Si-Yu Zhao ◽

Ya-Dong Yang ◽

Geng Yang ◽

Wen-Yuan Zhang ◽

...

Keyword(s):

Mechanical Properties ◽

Simulated Body Fluid ◽

Body Fluid ◽

Degradation Rate ◽

Maximum Load ◽

Artificial Ligament ◽

In Vivo Degradation

To investigate the degradation, mechanical properties, and histocompatibility of weft-knitted silk mesh-like grafts, we carried out the In Vitro and In Vivo silk grafts degradation assay. The In Vitro degradation experiment was performed by immersing the silk grafts in simulated body fluid for 1 year, and the results showed that the degradation rate of the silk mesh-like grafts was very slow, and there were few changes in the mechanical properties and quality of the silk mesh-like graft. In Vivo degradation assay was taken by implantation of the silk mesh-like grafts into the subcutaneous muscles of rabbits. At 3, 6, and 12 months postoperation, the rate of mass loss was 19.36%, 31.84%, and 58.77%, respectively, and the maximum load was 63.85%, 34.63%, and 10.76%, respectively of that prior to degradation. The results showed that the degradation rate of the silk graft and the loss of mechanical properties In Vivo were faster than the results obtained in the In Vitro experiments. In addition, there were no significant differences in secretion of serum IL-6 and TNF-α between the experimental and normal rabbits (P >0.05), suggesting no obvious inflammatory reaction. The findings suggest that the weft-knitted silk mesh-like grafts have good mechanical properties, histocompatibility, and In Vivo degradation rate, and therefore represent a candidate material for artificial ligament

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Bioactivity of Silver Doped Hydroxyapatite Scaffolds in Simulated Body Fluids

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.604.175 ◽

2014 ◽

Vol 604 ◽

pp. 175-179 ◽

Cited By ~ 1

Author(s):

Lasma Poca ◽

Arita Dubnika ◽

Dagnija Loca ◽

Liga Berzina-Cimdina

Keyword(s):

Simulated Body Fluid ◽

Incubation Period ◽

Powder Diffraction ◽

Body Fluid ◽

Body Fluids ◽

Apatite Layer ◽

X Ray ◽

Simulated Body Fluids ◽

Two Phases

In the present study, thein vitrobioactivity of silver-doped hydroxyapatite (HAp/Ag) scaffolds was investigated. HAp/Ag was prepared using two different modified wet precipitation methods. The X-ray powder diffraction (XRD) results showed, that sintered HAp/Ag samples prepared using method (I) contain two phases HAp and Ag, but samples prepared by method (II) contain three different phases - HAp, Ag and AgO. After 2 month incubation period in simulated body fluid (SBF), surface of HAp/Ag scaffolds was coated with bone-like apatite. Thickness of bone-like apatite layer increased from 2 μm up to 32 μm, increasing the incubation period.

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