Antibacterial Effect of Gold Nanoparticles Coated Dental Floss against Cariogenic Bacteria

2021 ◽  
Vol 904 ◽  
pp. 293-300
Author(s):  
Nattakan Traitanon ◽  
Surachai Dechkunakorn ◽  
Pornpen Tantivitayakul ◽  
Primana Punnakitikashem ◽  
Wassana Wichai ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Brain Heart Infusion ◽  
Blood Agar ◽  
Streptococcus Sobrinus ◽  
Posterior Teeth ◽  
Dental Floss ◽  
Cariogenic Bacteria ◽  
Additional Tool ◽  
Broth Microdilution Method ◽  
Inhibition Zones

Background: Carious lesions can occur on the proximal surfaces of the posterior teeth. Streptococcus mutans and Streptococcus sobrinus, are the main acidogenic bacteria that are commonly associated to dental caries. Interproximal cleaning is an important form of oral self-care habits, considering such areas of the dentition are easily affected by caries. Accordingly, dental floss has been used as an additional tool to enhance the quality of the cleaning process. It is reasonable that dental flossing should reduce interproximal caries risk because it is capable of removing parts of the interproximal plaque. The aim of this study was to evaluate the antibacterial activity of gold nanoparticle (AuNPs), when coated on unwaxed dental floss.Methods: Streptococcus mutans and Streptococcus sobrinus were cultured in Brain Heart Infusion (BHI) agar. Broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of the AuNPs with subcultures so obtained. Then, the bacteria were grown and spread on blood agar on which identical lengths (20mm) of unwaxed dental floss coated with gold nanoparticles (AuNPs) at concentration of 0.05, 0.1, and 0.5 mg/mL were placed. Control included untreated unwaxed dental floss and unwaxed dental floss with 0.2% Chlorhexidine (CHX). Five randomized sites of the inhibition zones were measured in millimeters in each concentration per floss. Means ± S.D. of the inhibition zones were calculated.Results: The MIC and MBC of AuNPs against S. sobrinus and S. mutans were 0.5 μg/mL and 1.0 μg/mL, respectively. The results indicated that gold nanoparticles (AuNPs) coated unwaxed dental floss, placed on blood agar with S. mutans and S. sobrinus demonstrated significant inhibition of bacterial growth. Gold nanoparticles (AuNPs) coated unwaxed dental floss at 0.05, 0.1, 0.5 mg/mL resulted in zones of inhibition ranging from 2.93 ± 0.30 mm to 4.71 ± 0.32 mm for S. sobrinus and 2.95 ± 1.73 mm to 5.18 ± 0.61 mm for S. mutans, respectively.Conclusion: Invitro study demonstrated that the AuNPs-coated unwaxed dental floss had antibacterial activities against cariogenic bacteria.

scholarly journals Antimicrobial Activity of the Essential Oil ofPlectranthus neochilusagainst Cariogenic Bacteria

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Eduardo José Crevelin ◽  
Soraya Carolina Caixeta ◽  
Herbert Júnior Dias ◽  
Milton Groppo ◽  
Wilson Roberto Cunha ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Chemical Constituents ◽  
Moderate Activity ◽  
Significant Activity ◽  
Streptococcus Sobrinus ◽  
Cariogenic Bacteria ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Major Chemical

This work used the broth microdilution method to investigate the antimicrobial activity of the essential oil obtained from the leaves ofPlectranthus neochilus(PN-EO) against a representative panel of oral pathogens. We assessed the antimicrobial activity of this oil in terms of the minimum inhibitory concentration (MIC). PN-EO displayed moderate activity againstEnterococcus faecalis(MIC = 250 μg/mL) andStreptococcus salivarus(MIC = 250 μg/mL), significant activity againstStreptococcus sobrinus(MIC = 62.5 μg/mL),Streptococcus sanguinis(MIC = 62.5 μg/mL),Streptococcus mitis(MIC = 31.25 μg/mL), andLactobacillus casei(MIC = 31.25 μg/mL), and interesting activity againstStreptococcus mutans(MIC = 3.9 μg/mL). GC-FID and GC-MS helped to identify thirty-one compounds in PN-EO;α-pinene (1, 14.1%),β-pinene (2, 7.1%),trans-caryophyllene (3, 29.8%), and caryophyllene oxide (4, 12.8%) were the major chemical constituents of this essential oil. When tested alone, compounds1,2,3, and4were inactive (MIC > 4000 μg/mL) against all the microorganisms. These results suggested that the essential oil extracted from the leaves ofPlectranthus neochilusdisplays promising activity against most of the evaluated cariogenic bacteria, especiallyS. mutans.

scholarly journals Effects of Baseplates of Orthodontic Appliances with in situ generated Silver Nanoparticles on Cariogenic Bacteria: A Randomized, Doubleblind Cross-over Clinical Trial

2015 ◽  
Vol 16 (4) ◽  
pp. 291-298 ◽  
Author(s):  
Roghayeh Ghorbanzadeh ◽  
Babak Pourakbari
Keyword(s):  
Clinical Trial ◽  
Silver Nanoparticles ◽  
Biofilm Formation ◽  
Orthodontic Appliances ◽  
Bacterial Cells ◽  
Streptococcus Sobrinus ◽  
Double Blind ◽  
Biofilm Inhibition ◽  
Cariogenic Bacteria

ABSTRACT Aim Polymethyl-methacrylate (PMMA) is commonly used primarily for baseplates of orthodontic appliances (BOA). The activities of cariogenic bacteria in biofilm on these surfaces may contribute to dental caries, gingival inflammation and periodontal disease. The PMMA incorporated with nanoparticles of silver (NanoAg-I-PMMA) and NanoAg in situ in PMMA (NanoAg-IS-PMMA) have been shown to control the growth of cariogenic bacteria, but clinical trial of anti-cariogenic application of these novel materials in orthodontics has not been evaluated. The main aim of the study is to compare the clinical effectiveness of using NanoAg-IS-PMMA and NanoAg-I-PMMA for construction of new BOA in inhibiting the planktonic growth and biofilm formation of the cariogenic bacteria. Materials and methods Twenty four patients with a median age of 12.6 years (7-15) harboring Streptococcus mutans, Streptococcus sobrinus and Lactobacillus acidophilus as well as Lactobacillus casei participated in the randomized, doubleblind, cross-over study. The experimental BOA, NanoAg-ISBOA and NanoAg-I-BOA, contained 0.5% w/w NanoAg while the control BOA was standard PMMA. Antibacterial effect of NanoAg-IS-BOA and NanoAg-I-BOA was assessed against test cariogenic bacteria by planktonic and biofilm bacterial cells growth inhibition. Results The average levels of test cariogenic bacteria in saliva decreased about 2 to 70 fold (30.9-98.4%) compared to baseline depending on the microorganism type and test BOA. Biofilm inhibition analysis demonstrated that NanoAg-I-BOA and NanoAg-IS-BOA inhibited the biofilm of all test bacteria by 20.1 to 79.9% compared to BOA. NanoAg-IS-BOA had a strong anti-biofilm effect against S. mutans, S. sobrinus and L. casei. However, NanoAg-I-BOA showed only slight antibiofilm effects on test bacteria. Most notably, at all period of the clinical trial, NanoAg-IS-BOA showed a higher antibacterial activity than NanoAg-I-BOA. Conclusion Based on the novel data that presented here, the NanoAg-IS-BOA had strong antimicrobial activity in the planktonic phase and subsequent biofilm formation of the cariogenic bacteria. Clinical significance Wearing of NanoAg-IS-BOA has the potential to minimize dental plaque formation and caries during orthodontic treatment. How to cite this article Ghorbanzadeh R, Pourakbari B, Bahador A. Effects of Baseplates of Orthodontic Appliances with in situ generated Silver Nanoparticles on Cariogenic Bacteria: A Randomized, Double-blind Cross-over Clinical Trial. J Contemp Dent Pract 2015;16(4):291-298.

scholarly journals Green ultrasonic synthesis, Characterization and Antibacterial activity of Silver and Gold Nanoparticles mediated by Ganoderma lucidum extract

2020 ◽  
Vol 4 (2) ◽  
Keyword(s):  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Metal Nanoparticles ◽  
Cytotoxic Activities ◽  
Gold And Silver Nanoparticles ◽  
Silver And Gold Nanoparticles ◽  
E Coli ◽  
Inhibition Zones

Metal nanoparticles possess an extensive scientific and technological significance due to their unique physiochemical properties and their potential applications in different fields like medicine. Silver and gold nanoparticles have shown to have antibacterial and cytotoxic activities. Conventional methods used in the synthesis of the metal nanoparticles involve use of toxic chemicals making them unsuitable for use in medical field. In our continued effort to explore for simple and eco-friendly methods to synthesize the metal nanoparticles, we here describe synthesis and characterization of gold and silver nanoparticles using Gonaderma lucidum, wild non-edible medicinal mushroom. G. lucidum mushroom contain bioactive compounds which can be involved in the reduction, capping and stabilization of the nanoparticles. Antibacterial activity analysis was done on E. coli and S. aureus. The synthesis was done on ultrasonic bath. Characterization of the metal nanoparticles was done by UV-VIS., High Resolution Transmission Electron Microscope (HRTEM) and FTIR. HRTEM analysis showed that both silver and gold nanoparticles were spherical in shape with an average size of 15.82±3.69 nm for silver and 24.73±5.124nm for gold nanoparticles (AuNPs). FTIR analysis showed OH and -C=C- stretching vibrations, an indication of presence of functional groups of biomolecules capping both gold and silver nanoparticles. AgNPs showed inhibition zones of 15.5±0.09mm and 13.3±0.14mm while AuNPs had inhibition zones of 14.510±0.35 and 13.3±0.50mm on E. coli and S. aureus respectively. The findings indicate the potential use of AgNPs and AuNPs in development of drugs in management of pathogenic bacteria.

scholarly journals A public health concern: emergence of carbapenem-resistant Klebsiella pneumoniae in a public transportation environment

2020 ◽  
Vol 75 (10) ◽  
pp. 2769-2772
Author(s):  
Tingting Cao ◽  
Yuanyuan Liu ◽  
Yiming Li ◽  
Yang Wang ◽  
Zhangqi Shen ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Public Transportation ◽  
Genetic Relationships ◽  
Brain Heart Infusion ◽  
Health Concern ◽  
Resistant Bacteria ◽  
Carbapenem Resistant ◽  
Broth Microdilution Method ◽  
Carbapenem Resistant Enterobacteriaceae ◽  
Beijing Subway

Abstract Objectives This study was designed to understand the prevalence of antibiotic-resistant bacteria in the Beijing subway environment and the potential transmission of carbapenem-resistant Enterobacteriaceae in a public transportation environment. Methods Carbapenem-resistant isolates were selected on brain heart infusion agar supplemented with meropenem (0.5 mg/L) and antimicrobial susceptibility testing was conducted using the broth microdilution method. WGS analyses were conducted for 11 Klebsiella pneumoniae isolates to identify resistance genes. The genetic relationships among the isolates were evaluated by MLST and PFGE. Results We identified 11 carbapenem-resistant K. pneumoniae isolates from the Beijing subway environment. WGS revealed three STs among the 11 isolates, with 9 isolates classified as ST726 and containing a blaNDM-5-carrying IncX3 plasmid. The genetic environment of blaNDM-5 was very similar to that observed in other blaNDM-5-containing clinical isolates. Conclusions The presence of carbapenem-resistant Enterobacteriaceae in a public transportation environment is concerning and indicates that regular antimicrobial resistance surveillance is urgent and necessary.

scholarly journals Response of Group B Streptococcus to the Combination of Gold Nanoparticles-Erythromycin as a Nanoweapon

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Leila Fozouni ◽  
Prastoo Vaezi ◽  
Ania Ahani Azari
Keyword(s):  
Gold Nanoparticles ◽  
Pregnant Women ◽  
Rural Areas ◽  
Group B Streptococcus ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Broth Microdilution ◽  
Wide Range ◽  
Broth Microdilution Method ◽  
Group B

Background: Group B Streptococcus (GBS) causes a wide range of adverse effects in both mothers and infants during pregnancy and after delivery. Objectives: This study aimed to evaluate the effects of erythromycin either alone or in combination with gold nanoparticles (AuNPs) on the clinical GBS isolated from pregnant women. Methods: This descriptive cross-sectional study was performed on 106 women aged 16 - 48 years. After identification of GBS strains by phenotypic and genotypic methods (PCR), erythromycin-resistant isolates were identified using the Kirby-Bauer test and broth microdilution method according to CLSI-2015 guidelines. The antibacterial properties and minimum inhibitory concentration (MIC) of erythromycin (either alone or combined with AuNPs) were assessed by the agar well-diffusion and broth microdilution methods, respectively. Results: The frequency of GBS isolates was significantly high in the pregnant women aged less than 40 years (73.9%) (P = 0.0251), those with a history of abortion (60.9%) (P = 0.038), and residents of rural areas (60%) (P = 0.038). Moreover, 65.2% of the isolates were resistant to erythromycin. The MIC of AuNPs-erythromycin combination required to inhibit the growth of 50% of GBS isolates (MIC50 = 0.25 μg/mL) was significantly lower than the concentration of AuNP-erythromycin required to inhibit the growth of 90% of the isolates (MIC90 = 1 μg/mL) (P = 0.02), indicating a 16-fold lower dose than the values for erythromycin and AuNPs alone. In the agar well-diffusion method, the average diameter of the growth inhibition zone of AuNPs-erythromycin was 2.5-fold greater than that of free erythromycin (P = 0.037). Conclusions: The results showed that the combination of erythromycin with AuNPs increased the antibacterial effects of erythromycin against GBS isolates.

AGE RELATED PECULIARITY OF THE CONTACT POINTS OF THE POSTERIOR TEETH FROM THE POINT OF VIEW OF RESTORATIVE DENTISTRY

2021 ◽  
pp. 95-102
Author(s):  
Ginali A.N. ◽  
Nikolaev A.I. ◽  
Permyakova A.V.
Keyword(s):  
Surface Area ◽  
Age Groups ◽  
Point Of View ◽  
Special Technique ◽  
Posterior Teeth ◽  
Dental Floss ◽  
Contact Areas ◽  
Contact Points ◽  
Age Related ◽  
Direct Restoration

Reconstruction of the age-related anatomical and physiological features of the contact surfaces and contact points inherent in natural teeth in the treatment of caries of posterior teeth with a direct restoration with light-cured composite is one of the unsolved problems of practical dentistry. The study is devoted to the determination of age-related characteristics of the localization and shape of contact points of posterior teeth in permanent dentition. The assessment of localization and shape of 1224 contact points of posterior teeth in 236 patients aged 20 to 59 years was carried out. Comparison of data about the surface area, shape, vertical and horizontal positions of contact points related to teeth anatomic structures in the interdental space was performed. The analysis of interproximal intraoral radiography data, examination and measurement of the parameters of interdental contacts using dental floss, analysis of the display of contact points on dental impressions made according to a special technique were carried out. The localization of the contact areas of the posterior teeth with a displacement in the vestibular direction, the associated asymmetry of the buccal and lingual (palatal) interdental embrasures, as well as a gradual increase in the surface area of contact areas and a decrease in the depth of the occlusal interdental embrasures, correlated with the age of the patients, were recorded. The average numerical values of the listed parameters are derived, taking into account the age of the patients. Anatomical and topographic features of the interdental spaces of the posterior teeth in adult patients are characterized by the following features: the contact areas of the posterior teeth are displaced in the vestibular direction, the buccal and lingual (palatal) interdental embrasures are asymmetric; dynamics of the depth of occlusal interdental embrasures, linear dimensions and area of contact areas is expressed as average digital values for different age groups: 20-40 years: contact areas – 1×2 mm, S = 2 mm2, depth of occlusal interdental embrasure – 1.5 mm; over 40 years old: contact pads – 1.5 × 3 mm, S = 4.5 mm2, the depth of the occlusal interdental embrasure – 1.0 mm.

Evaluation of enrichment, storage, and age of blood agar medium in relation to its ability to support growth of anaerobic bacteria

1976 ◽  
Vol 4 (5) ◽  
pp. 394-399
Author(s):  
C W Hanson ◽  
W J Martin
Keyword(s):  
Colony Size ◽  
Agar Medium ◽  
Anaerobic Bacteria ◽  
Basal Medium ◽  
Brain Heart Infusion ◽  
Storage Conditions ◽  
Blood Agar ◽  
Minor Effect ◽  
Rich Media ◽  
A Minor

By measuring the colony size of a variety of anaerobic bacteria isolated from clinical specimens, an evaluation was made of the benefits derived from the addition of several enrichments to blood agar medium commonly used for the growth of anaerobes. Similar methods were used to study the effects of various storage conditions and age of the medium. The results were compared with those obtained on freshly prepared and enriched blood agar plates as well as commercially available blood agar plates. Freshly prepared and enriched blood agar was found to give substantially larger colonies than could be grown on commercially obtained blood agar plates when both were inoculated and incubated under identical conditions. Storage of plating media under CO2 for periods of up to 72 h had only a minor effect on the growth of the anaerobic bacteria studied, but longer periods of storage under CO2 resulted in a less efficient plating medium. Nonenriched brain heart infusion (BHI) was found to be a better basal medium than Trypticase soy agar (TSA) medium. Colony size on fully enriched BHI blood agar plates was greater than nonenriched BHI greater than nonenriched TSA greater than commercially prepared nonenriched TSA plates. The data suggest that freshness of the plates may be as important as using rich media.

PENURUNAN PRODUKSI ASAM DAN PERTUMBUHAN BAKTERI STREPTOCOCCUS SOBRINUS SETELAH TERPAPAR REBUSAN DAUN SIRIH MERAH 10%

2013 ◽  
Vol 17 (4) ◽  
pp. 324-328
Author(s):  
Tetiana Haniastuti ◽  
Ristini Asih
Keyword(s):  
Brain Heart Infusion ◽  
Brain Heart Infusion Broth ◽  
Streptococcus Sobrinus

Karies merupakan penyakit infeksi yang disebabkan oleh bakteri kariogenik yang terdapat di dalam rongga mulut, antara lain Streptococcus sobrinus. Bakteri tersebut mampu menghasilkan asamorganik yang menyebabkan demineralisasi email gigi, sehingga menghancurkan jaringan keras gigi. Sirih merah merupakan salah satu tanaman yang banyak ditanam oleh masyarakat Indonesia. Secara tradisional, rebusan daun sirih merah sering digunakan oleh masyarakat untuk mengobati penyakit gigi dan mulut. Penelitian ini bertujuan untuk mengetahui potensi antikariogenik rebusan daun sirih merah dengan menguji produksi asam dan pertumbuhan Streptococcus sobrinus setelah terpapar rebusan daun sirih merah. Produksi asam dan laju pertumbuhan diuji dengan menambahkan rebusan daun sirih merah konsentrasi 5% dan 10% serta akuades (kontrol negatif) dan Listerine(kontrol positif) pada media brain heart infusion broth yang mengandung 1% glukosa. Media kemudian diinokulasi dengan 106 sel bakteri dan diinkubasi pada suhu 37oC selama1, 2, 4, 6, dan 8 jam. Absorbansi media diukur secara spektrofotometri pada panjang gelombang 550 nm, sedangkan pH media ditentukan dengan menggunakan pH-meter. Hasil uji Kruskal Wallismenunjukkan bahwa tidak terdapat perbedaan pH yang signifikan antar kelompok sesaat setelah perlakuan (p> 0,05). Hasil uji Mann Whitney menunjukkan bahwa kelompok yang terpapar rebusan daun sirih merah 10% mempunyai pH yang secara signifikan lebih tinggi (p< 0,05) dibandingkan kelompok yang lain, mengindikasikan bahwa paparan rebusan daun sirih merah 10% mampu menurunkan produksi asam dari S. sobrinus. Setelah 24 jam, jumlah koloni bakteri S. sobrinus pada kelompok yang terpapar rebusan daun sirih merah 10% secara signifikan lebih sedikit dibandingkan kelompok kontrol negative (p< 0,05), tapi secara signifikan lebih banyak dibandingkan kelompok kontrol positif (p< 0,05). Hal ini mengindikasikan bahwa paparan rebusan daun sirih merah 10% dapat menurunkan jumlah koloni S. sobrinus. Dari hasil penelitian ini disimpulkan bahwa rebusan daun sirih merah 10% mempunyai potensi antikariogenik karena mempunyai kemampuan menurunkan produksi asam dan laju pertumbuhan S. sobrinus.

Sign in / Sign up

Export Citation Format

Share Document