FATTY ACID COMPOSITION AND LIPID CONTENT OF LATE LARVAL AND PUPAL STAGES OF THE EASTERN HEMLOCK LOOPER, LAMBDINA FISCELLARIA FISCELLARIA (LEPIDOPTERA: GEOMETRIDAE)

1977 ◽  
Vol 109 (3) ◽  
pp. 347-350 ◽  
Author(s):  
Gary B. Dunphy ◽  
Kevin M. W. Keough ◽  
Richard A. Nolan

AbstractA total of 14 known fatty acids and one unknown fatty acid were detected in the third instar, fourth instar, prepupa, and female and male pupae of the eastern hemlock looper, Lambdina fiscellaria fiscellaria. The C8:0,3 C10:0, C22:1, and C24:0 fatty acids and an unknown fatty acid were not detected in the third instar larvae, and the unknown was also absent from the fourth instar larvae. The C24:0 fatty acid was absent from the prepupae and both the male and female pupae. The C22:1 fatty acid was absent from the prepupae. Otherwise, developmental stages contained the C8:0, C10:0, C14:0, C15:0, C16:0, C18:0, C18:1, C18:2, C18:3,C20:0, C20:1, C22:0, C22:1, and the C24:0 fatty acids and the unknown fatty acid. In general, the level of a given fatty acid decreased with succeeding developmental stages. The lipid level was also determined for each developmental stage.


Biologia ◽  
2007 ◽  
Vol 62 (6) ◽  
Author(s):  
Ozlem Cakmak ◽  
Mehmet Bashan ◽  
Ali Satar

AbstractTotal lipid and the fatty acid compositions of phospholipid and triacylglycerol fractions, prepared from eggs, 3rd instars of larvae, pupae, male and female adults of Lertha sheppardi, were analyzed by gas chromatography and gas chromatography-mass spectrometry. The effect of diet (adults’ nutrition) on fatty acid composition of L. sheppardi adults was also investigated. Total lipid of L. sheppardi considerably increased in adults compared with immature stages. There was a significant decrease in total lipid level in larval stage in contrast with egg stage. Qualitative analysis revealed the presence of 14 fatty acids during all stages. The major components were C16 and C18 saturated and unsaturated components which are ubiquitous to most animal species. In addition to these components, one odd-chain (C17:0) and prostaglandin precursor fatty acids were found. The fatty acid profiles of phospholipids and triacylglycerols were substantially different. In phospholipid fraction, monounsaturated fatty acids were the major proportion of fatty acids in both sex of adults and pupae, whereas polyunsaturated fatty acids were the most dominant fatty acids in eggs and 3rd instars. Results of triacylglycerol fraction revealed that fatty acid composition of eggs had higher level of C16:1, C18:0 and C18:3n-3 content than that of 3rd instars and pupae, which suggests accumulation of energetic and structural reserve materials during embryonic development. At more advanced developmental stages, mainly in adult females, the amount of C16:1 increased once again, which may be related to the need for accumulation of sufficient energy and of carbon reservoir in the developing new vitellum. Percentages of C18:1 were significantly high in adult stages compared to other stages. These findings indicate that the accumulation and consumption of fatty acids fluctuate through different development stages. Diet did not effect the fatty acid composition of L. sheppardi adults.



2020 ◽  
Vol 12 (16) ◽  
pp. 6320 ◽  
Author(s):  
Radisav Dubljević ◽  
Božidarka Marković ◽  
Dušica Radonjić ◽  
Danijela Stešević ◽  
Milan Marković

Natural pastures in Montenegro, together with natural meadows, are the most important forage source and comprise 95% of the used agricultural area (UAA). The aim of this study was to estimate the changes in grassland biomass, chemical and floristic composition and fatty acid composition in milk of cows grazed on Agrostis stolonifera semi-natural wet grassland in the Central region of Montenegro. Investigation was conducted during three phenological phases: early vegetative, optimal vegetative and full reproductive phase. The chemical composition of the grassland biomass was changed through the phenological phases. A significant increase during the experimental period in the content of dry matter and crude fibers and a decrease in crude protein content were determined. The pasture community consisted of 52 species, belonging to 19 families. The total amount of plant species classified as excellent, very good and good quality was 7.69%, while medium quality plants amounted to 13.46%, low quality plants to 19.23%, valueless plants to 34.62% and toxic plants to 25%. The total quality index was 4.166. The highest quality index of this community was in the second phenological phase, then in the third phase the lowest quality was in the first phenological phase. According to the floristic composition and biomass quality, this lowland pasture can be qualified as being of moderate to good quality. The content of milk fat, protein and solid non-fat (SNF) was increased from the first to the third vegetation phase, but the differences between the phases were not significant (p > 0.05). The content of a majority of saturated fatty acids (SFAs) also increased, while the total content of monounsaturated fatty acids (MUFAs) was high in the second phase and decreased significantly in the third phase. The content of polyunsaturated fatty acids (PUFAs) was constant in the first two phases, while it significantly decreased in the third phase. The highest content of most of MUFAs and PUFAs was obtained in the second phenological phase. The content of fatty acids in the third phase significantly affected (p < 0.05) by the phenological phase. This research contributes to the improvements in the grazing management of wet grasslands as well as the animal performances (milk yield and quality of milk).



2022 ◽  
Vol 2022 ◽  
pp. 1-10
Author(s):  
Rebecca Coughlan ◽  
Siobhan Moane ◽  
Tracey Larkin

The low saturated fatty acid content of rapeseed oil has resulted in it being classed as one of the most health-benefiting culinary oils. This study determines whether Irish rapeseed oils contain identical fatty acid profiles or whether distinct profiles exist between producers and producers’ successive oil batches. The fatty acid content of Irish rapeseed oils was determined in terms of the desirable MUFA and PUFA and saturated content of these oils. The fatty acid composition demonstrated significant differences in individual unsaturated fatty acid content, while total saturation had insignificant differences. Saturated fatty acid content ranged from 6.10 to 15.8%, while unsaturated fatty acids ranged from 84.20 to 90.10%. Moreover, individual fatty acid content exhibited significant differences ( p < 0.05 ). Oleic acid (C18:1), linoleic acid (C18:2), and stearic acid (C18:0) contents were considered significantly different from other fatty acids detected. The third successive batch from each producer exhibited lower oleic acid content, and the third batch contained higher linoleic acid content, at the same time maintaining a desirable unsaturated fatty acid composition. Studies suggest that differences in the fatty acid composition may be due to cultivation practices such as climate, soil composition, sowing and harvesting, processing techniques, and oxidation reactions.



2007 ◽  
Vol 23 (5-6-1) ◽  
pp. 467-474
Author(s):  
M. Furman ◽  
T. Polak ◽  
S. Vidakovic ◽  
L. Gasperlin ◽  
B. Zlender

The aim of the study was to investigate the effect of diet and sex on fat content, fatty acid composition and cholesterol content of dried pork neck. There were 12 barrows and 12 gilts separated in 4 groups regarding different nutritive supplement. The first group was fed basic ratio with supplement of linseed, the second with canola, the third with canola and vitamin E and the forth group without nutritive supplement (control group). All 24 samples of dried neck were analyzed in laboratory. There was no effect of nutritive supplement on fat and cholesterol content. Diet with linseed resulted in higher ?-linolenic fatty acid content in dried neck. Canola diet resulted in higher oleic and linoleic acid contents. Barrows dried necks were fattier than gilts, but there were no difference in cholesterol content. Gilts had more polyunsaturated fatty acids and less saturated fatty acids than barrows.



1981 ◽  
Vol 27 (7) ◽  
pp. 670-674 ◽  
Author(s):  
Gary B. Dunphy ◽  
K. M. W. Keough ◽  
Richard A. Nolan

The major fatty acids (> 10%) of Entomophthora egressa were C16:0 and C18:1. Minor fatty acids, which varied with the stage of fungal development, included C11:0, C12:0, C13:0, C14:0, C15:0, C16:1, C17:0, C18:0, C18:2, C18:3, C20:1, C20:2, C20:3, C20:4, C20:5, and two unidentified unsaturated fatty acids. Differences were observed between the total fatty acid levels of C12:0, C14:0, C17:0, C18:0, and C20:5 and the degree of unsaturation of the fatty acids of 37-h protoplasts grown in modified Grace's medium and a simplified growth medium (SGM). The levels of C12:0, C14:0, C18:1, C20:4, and C20:5 decreased and the levels of C18:0 and C20:2 increased with the formation of spherical hyphal body (shb) initials. With the production of mature shb increased levels of C12:0, C14:0, C15:0, C18:1, C20:4, and C20:5 were detected. During the germination of the shb the levels of C14:0, C16:1, C18:1, and C20:4 increased, whereas C15:0 and C20:5 levels declined. The fatty acid levels, except for Cl2:0, C13:0, and C20:2, remained constant during the mycelial stage. The degree of fatty acid unsaturation decreased during early stages of development (protoplasts through shb initials). In SGM the degree of fatty acid unsaturation was lowest during the shb initial stage and highest during the shb stage. The total lipid level increased during shb maturation and declined during shb germination.



1985 ◽  
Vol 54 (03) ◽  
pp. 563-569 ◽  
Author(s):  
M K Salo ◽  
E Vartiainen ◽  
P Puska ◽  
T Nikkari

SummaryPlatelet aggregation and its relation to fatty acid composition of platelets, plasma and adipose tissue was determined in 196 randomly selected, free-living, 40-49-year-old men in two regions of Finland (east and southwest) with a nearly twofold difference in the IHD rate.There were no significant east-southwest differences in platelet aggregation induced with ADP, thrombin or epinephrine. ADP-induced platelet secondary aggregation showed significant negative associations with all C20-C22 ω3-fatty acids in platelets (r = -0.26 - -0.40) and with the platelet 20: 5ω3/20: 4ω 6 and ω3/ ω6 ratios, but significant positive correlations with the contents of 18:2 in adipose tissue (r = 0.20) and plasma triglycerides (TG) (r = 0.29). Epinephrine-induced aggregation correlated negatively with 20: 5ω 3 in plasma cholesteryl esters (CE) (r = -0.23) and TG (r = -0.29), and positively with the total percentage of saturated fatty acids in platelets (r = 0.33), but had no significant correlations with any of the ω6-fatty acids. Thrombin-induced aggregation correlated negatively with the ω3/6ω ratio in adipose tissue (r = -0.25) and the 20: 3ω6/20: 4ω 6 ratio in plasma CE (r = -0.27) and free fatty acids (FFA) (r = -0.23), and positively with adipose tissue 18:2 (r = 0.23) and 20:4ω6 (r = 0.22) in plasma phospholipids (PL).The percentages of prostanoid precursors in platelet lipids, i. e. 20: 3ω 6, 20: 4ω 6 and 20 :5ω 3, correlated best with the same fatty acids in plasma CE (r = 0.32 - 0.77) and PL (r = 0.28 - 0.74). Platelet 20: 5ω 3 had highly significant negative correlations with the percentage of 18:2 in adipose tissue and all plasma lipid fractions (r = -0.35 - -0.44).These results suggest that, among a free-living population, relatively small changes in the fatty acid composition of plasma and platelets may be reflected in significant differences in platelet aggregation, and that an increase in linoleate-rich vegetable fat in the diet may not affect platelet function favourably unless it is accompanied by an adequate supply of ω3 fatty acids.



Author(s):  
Mohamed Haniff Hanafy Idris ◽  
Yanty Noorzianna Manaf ◽  
Mohd Nasir Mohd Desa ◽  
Amalia Mohd Hashim ◽  
Muhamad Shirwan Abdullah Sani ◽  
...  


Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 941
Author(s):  
Ewa Szpunar-Krok ◽  
Anna Wondołowska-Grabowska ◽  
Dorota Bobrecka-Jamro ◽  
Marta Jańczak-Pieniążek ◽  
Andrzej Kotecki ◽  
...  

Soybean is a valuable protein and oilseed crop ranked among the most significant of the major crops. Field experiments were carried out in 2016–2019 in South-East Poland. The influence of soybean cultivars (Aldana, Annushka), nitrogen fertilizer (0, 30, 60 kg∙ha−1 N) and inoculation with B. japonicum (control, HiStick® Soy, Nitragina) on the content of fatty acids (FA) in soybean seeds was investigated in a three-factorial experiment. This study confirms the genetic determinants of fatty acid composition in soybean seeds and their differential accumulation levels for C16:0, C16:1, C18:1n9, C18:2, C18:3, and C20:0 as well saturated (SFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids. Increasing the rate from 30 to 60 kg ha−1 N did not produce the expected changes, suggesting the use of only a “starter” rate of 30 kg ha−1 N. Inoculation of soybean seeds with a strain of Bradyrhizobium japonicum (HiStick® Soy, BASF, Littlehampton, UK and Nitragina, Institute of Soil Science and Plant Cultivation–State Research Institute, Puławy, Poland) is recommended as it will cause a decrease in SFA and C16:0 acid levels. This is considered nutritionally beneficial as its contribution to total fatty acids determines the hypercholesterolemic index, and it is the third most accumulated fatty acid in soybean seeds. The interaction of cultivars and inoculation formulation on fatty acid content of soybean seeds was demonstrated. An increase in the value of C16:0 content resulted in a decrease in the accumulation of C18:1, C18:2, and C18:3 acids. The content of each decreased by almost one unit for every 1% increase in C16:0 content. The dominant effect of weather conditions on the FA profile and C18:2n6/C18:3n3 ratio was demonstrated. This suggests a need for further evaluation of the genetic progress of soybean cultivars with respect to fatty acid composition and content under varying habitat conditions.





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