scholarly journals Suppression of Tumorigenesis: Modulation of Inflammatory Cytokines by Oral Administration of Microencapsulated Probiotic Yogurt Formulation

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Aleksandra Malgorzata Urbanska ◽  
Arghya Paul ◽  
Jasmine Bhahena ◽  
Satya Prakash
Keyword(s):  
Treatment Group ◽  
Intestinal Inflammation ◽  
Interleukin 1 ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Western Blots ◽  
Reactive Protein ◽  
Probiotic Yogurt ◽  
Factor Alpha ◽  
Irritable Bowel

The objective of this study was to examine the ability of a novel microencapsulated probiotic yogurt formulation to suppress the intestinal inflammation. We assessed its anticancer activity by screening interleukin-1, 6, and 12 (IL-1, 6, 12), secretory levels of tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), prostaglandinE2  (PGE2), and thromboxane B2 in the digesta obtained from the duodenum, jejunum, proximal, and distal segments of the ileum of C57BL/6J-ApcMin/J mice. Formulation-receiving animals showed consistently lower proinflammatory cytokines' levels when compared to control group animals receiving empty alginate-poly-L-lysine-alginate (APA) microcapsules suspended in saline. The concentrations of IL-12 found in serum in control and treatment group animals were significant:46.58±16.96 pg/mL and158.58±28.56 pg/mL for control and treatment animals, respectively. We determined a significant change in plasma C-reactive protein:81.04±23.73 ng/mL in control group and64.21±16.64 ng/mL in treatment group. Western blots showed a 71% downregulation of cyclooxygenase-2 (COX-2) protein in treatment group animals compared to control. These results point to the possibility of using this yogurt formulation in anticancer therapies, in addition to chronic gut diseases such as Crohn's disease, irritable bowel syndrome (IBS), and inflammatory bowel disease (IBD) thanks to its inflammation lowering properties.

scholarly journals Serum levels of inflammatory mediators as prognostic biomarker in silica exposed workers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
José Jesús Blanco-Pérez ◽  
Sara Blanco-Dorado ◽  
Javier Rodríguez-García ◽  
Mª Elena Gonzalez-Bello ◽  
Ángel Salgado-Barreira ◽  
...  
Keyword(s):  
Inflammatory Mediators ◽  
Transforming Growth Factor ◽  
Interleukin 1 ◽  
Interleukin 2 ◽  
Serum Levels ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Reactive Protein ◽  
Prediction Of Mortality ◽  
Alpha 1 Antitrypsin

AbstractSilicosis is a diffuse interstitial lung disease caused by sustained inhalation of silica and silicates. Several cytokines are activated by their inhalation and can mediate the process of pulmonary fibrosis. The identification of biomarkers could allow an early diagnosis before the development of radiological alterations and help monitor the evolution of patients. The objetive of this study was to determine the clinical significance of specific biomarkers, to estimate their association with the development, severity and/or progression of silicosis, and identify determinants of this evolution. We conducted a prospective observational study in patients attending the pulmonology clinic from 2009 to 2018. Serum levels of the following inflammatory mediators were assessed: interleukin-6 (IL-6), interleukin 2 receptor subunit alpha (IL2R) interleukin 1 beta (IL1B), interleukin-8 (IL-8), tumour necrosis factor-alpha (TNF-α), transforming growth factor-beta1 (TGF-β1), alpha-1 antitrypsin (AAT), C-reactive protein (CRP), lactate dehydrogenase (LDH) and ferritin in subjects exposed to silica, with and without silicosis. Association between those inflammatory mediators with lung function measurements and radiological severity of disease and their impact on prognosis were analysed. 337 exposed to silica (278 with silicosis) and 30 subjects in the control group were included. IL-8, α1AT, ferritin, CRP and LDH levels were higher in silicosis than in those exposed to silica without silicosis. IL-8, LDH and AAT levels were associated with progression of silicosis and IL-6, IL-8, LDH, AAT, ferritin, and CRP with vital status. The results of the ROC analysis indicated the potential of IL-8 as a biomarker in the presence of silicosis and for the prediction of mortality.

AdultBrugia malayimitochondrial and nuclear fractions impart Th1-associated sizeable protection against infective larval challenges inMastomys coucha

2009 ◽  
Vol 83 (1) ◽  
pp. 83-95 ◽  
Author(s):  
S. Shakya ◽  
A.K. Srivastava ◽  
S. Misra-Bhattacharya
Keyword(s):  
Interleukin 1 ◽  
Peritoneal Macrophages ◽  
Control Group ◽  
Rodent Host ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Antibody Isotypes ◽  
Factor Alpha ◽  
Mastomys Coucha

AbstractProtective immunity to the subperiodic human filariid,Brugia malayi, was explored in the rodent host,Mastomys couchaafter vaccination with subcellular fractions derived from the adult stage of the parasite. The highest level of protection was conferred in animals vaccinated with the ‘mitochondria rich’ (MT) fraction, in which microfilaraemia and worm burden were markedly reduced by 67.2 and 65.9%, respectively, followed by the ‘nucleus rich’ (NR) fraction, showing reductions of 62 and 52.3%, respectively, over the non-immunized control group. Mastomys vaccinated with MT and NR, displayed a significant increase in the level of antigen-specific serum immunoglobulin G (IgG). The levels of IgG2a, IgG2b and IgM antibody isotypes were remarkably elevated in both the MT and NR immunized groups, while IgG1 and IgG3 levels were low. Apart from antibodies, both these fractions also led to marked antigen-specific lymphoproliferationin vitro, along with enhanced release of nitric oxide by peritoneal macrophages. There was an increased population of CD4+ and CD8a+T-cells in MT immunized animals, as measured by flow cytometry, accompanied by elevated levels of proinflammatory cytokines; interferon gamma (IFN-γ), tumour necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β) in the culture supernatants of the activated splenocytes. The results suggest that both NR and MT contain proinflammatory molecules which evoke a protective Th1 type of immune response.

Simulation stress model of the undersea environment activates the central nervous, neuroendocrine and immune systems and anxiety in rats

2020 ◽  
pp. 445-453
Author(s):  
Ying Tang ◽  
Ying-Xin Zou ◽  
Wei Fan ◽  
Shuang-Hong Chen ◽  
Yi-Qun Fang ◽  
...  
Keyword(s):  
Stress Responses ◽  
Interleukin 1 ◽  
Control Group ◽  
Stress Model ◽  
Necrosis Factor Alpha ◽  
Immune Systems ◽  
Tnf Α ◽  
The Central Nervous System ◽  
Factor Alpha ◽  
Necrosis Factor

The present study was designed to assess the stress responses to a simulation model of the undersea environment that is similar to some undersea working conditions such as submarine rescue, underwater salvage and underwater construction. Restraint, hyperbaric air and immersion were chosen to produce the simulation stress model in rats for four hours. Rats were randomized into five groups: control group, restraint (R) group, hyperbaric air (H) group, restraint plus hyperbaric air (RH) group, and restraint plus hyperbaric air plus immersion (RHI) group. The results showed that the responses to the simulation stress model of the undersea environment induced by R, H, RH and RHI involved the upregulated norepinephrine (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT) of the central nervous system (CNS), upregulated adrenocorticotropic hormone (ACTH), corticosterone (CORT) and blood glucose of the neuroendocrine system, upregulated interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) of the immune system, and increased anxiety in rats. Compared with hyperbaric air, restraint tended to activate stronger stress responses. Conclusively, this work established a simulation stress model of the undersea environment induced by restraint, hyperbaric air and immersion. It further provided experimental data of such a model that showed significant activation of the CNS, neuroendocrine and immune systems and anxiety in rats. In this experiment we provided an experimental basis for undersea work such as working aboard a submarine.

scholarly journals The Value of Safflower Yellow Injection for the Treatment of Acute Cerebral Infarction: A Randomized Controlled Trial

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Le-Jun Li ◽  
Yu-Mei Li ◽  
Ben-Yu Qiao ◽  
Shan Jiang ◽  
Xin Li ◽  
...  
Keyword(s):  
Cerebral Infarction ◽  
Controlled Trial ◽  
Interleukin 1 ◽  
Acute Cerebral Infarction ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Once Daily ◽  
Rbc Aggregation ◽  
Factor Alpha ◽  
And Control

Background. Safflower Yellow Injection has been reported as a treatment for acute cerebral infarction in recent studies in China. However, there is a lack of availability of the evidence for the efficacy and safety of Safflower Yellow Injection for the treatment of acute ischemic stroke. So we investigated the effects of Safflower Yellow Injection for the treatment of acute cerebral infarction.Method. All subjects were randomly divided into Safflower Yellow Injection group given Safflower Yellow Injection (80 mg) and control group given placebo (0 mg) injection by intravenous drop once daily for 14 days. National Institute of Health Stroke Scale (NIHSS); hemorheological detection; coagulation function; and serum inflammatory markers, tumor necrosis factor-alpha (TNF-α), interleukin-1β(IL-1β), and interleukin-6 (IL-6), were used to investigate the effects before and 14 days after the treatment.Results. The scores of NIHSS were decreased on day 7 and day 14 after treatment. The hemorheological index of RBC deformation and RBC aggregation were significantly improved, prothrombin time (PT) increased, and fibrinogen (FIB) and TNF-α, IL-1β, and IL-6 were decreased in patients treated with Safflower Yellow injection on day 14 after treatment (P<0.05).Conclusion. Data suggests that Safflower Yellow Injection therapy may be beneficial for acute cerebral infarction.

scholarly journals SLEEP DISORDERS IN THE COVID-19 PANDEMIC: NARRATIVE REVIEW

2021 ◽  
Keyword(s):  
Interleukin 1 ◽  
Well Being ◽  
Quality Of Sleep ◽  
Necrosis Factor Alpha ◽  
Secondary Sources ◽  
Reactive Protein ◽  
Factor Alpha ◽  
The Individual ◽  
Sensory Processes

Sleep is a biological need that can be affected by various factors and interferes with physical and mental well-being. Thus, the current pandemic of COVID-19 has changed the routine of a large part of the population, as well as affected the quality of sleep and has become a risk factor for the development of problems. In this context, the present study sought to highlight the importance of sleep quality for different situations. In view of this, the article understands how the current pandemic scenario has interfered with the quality of sleep and, consequently, with the population’s biopsychosocial paradigm. This is a bibliographic review with data collection from secondary sources in March and April 2021, carried out in the SciELO and PubMed databases. It has been observed that sleep deprivation makes it possible to increase cytokine activity, namely interferon (IFN), tumor necrosis factor alpha (TNF-α), interleukin-1-beta (IL-1-beta), favoring the increase in inflammatory markers such as C-reactive protein (CRP), this set of changes negatively affects immunity and is directly related to changes in sleep patterns. Therewith, from the analysis, it is possible to perceive the harm that the scenario of COVID-19 brought to the quality of sleep, causing several losses in daily activities. Therefore, it can be inferred that changes related to sleep contribute to the absence of alertness and changes in perception as well as in sensory processes, making the individual lethargic and with a reduction in the cognitive ability to perform functions of essential attributions for their quality of life.

scholarly journals Efficiency of postoperated microwave therapy of the patients with inflammated tubo-ovarian tumors

2002 ◽  
Vol 1 (4) ◽  
pp. 80-87
Author(s):  
I. D. Evtushenko ◽  
A. P. Romanova
Keyword(s):  
Blood Serum ◽  
Interleukin 6 ◽  
Interleukin 1 ◽  
Cervical Canal ◽  
Necrosis Factor Alpha ◽  
Reactive Protein ◽  
Quantitative Characteristics ◽  
Factor Alpha ◽  
Microwave Therapy ◽  
Necrosis Factor

The aim of the research is the study of efficiency of early postoperative rehabilitation of patients with purulent inflammatory formations of uterine appendages with microwave therapy. Postoperative treatment of 36 women-patients with the use of combined skin and intravaginal microwave exposure was done. The clinical characteristics of postoperative period and the results of the therapy in 18 month were studied. Dynamical changes of quantitative characteristics of cells at leukemia, of C-reactive protein, content in blood serum, in cervical canal and endometrium of tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 were estimated. If comparing with the patients who didn’t get physiotherapy exposure the use of microwave therapy at the patients with purulent tubo-ovarian formations was followed by earlier disappearance of inflammation, restoration of quantitative characteristics of leucocytes in blood, normalization of C-reactive protein concentration in blood serum and normal characteristics of erythrocyte sedimentation rate. Diminution of tumor necrosis factor-alpha in blood serum, interleukin-1 beta and interleukin-6, reduction of concentration in cervical canal and endometrium interleukin-6 were observed. In the postoperative period under the microwave therapy womenpatients had pain in pelvis, disturbance of menstrual cycle, 26,5% of women had the reproductive function to be restored.

scholarly journals Estimation of Some Interleukins in Cerebrospinal Fluid in Children with Meningitis

2019 ◽  
Vol 12 (04) ◽  
pp. 2151-2155
Author(s):  
Entedhar R. Sarhat ◽  
Zubaidah N. M Albarzanji ◽  
Chateen I. Ali Pambuk
Keyword(s):  
Cerebrospinal Fluid ◽  
Serum Glucose ◽  
Control Group ◽  
Serum Total Protein ◽  
Necrosis Factor Alpha ◽  
Reactive Protein ◽  
Tnf Α ◽  
The Central Nervous System ◽  
Factor Alpha ◽  
Necrosis Factor

Meningitis is often associated with cerebral compromise which may be responsible for neurological squeal in nearly half of the survivors. Little is known about the mechanisms of CNS involvement in bacterial meningitis. the current study was to analyze the clinical significance of cerebrospinal fluid (CSF) concentrations of interleukin (IL)- 6, IL-1, IL-8, IL-10, tumor necrosis factor-alpha (TNF-α), and C-reactive protein in children with meningitis (n=35) and compared with control subjects (n=28). Serum total protein, interleukine-6, IL-1 , IL-8, IL-10 , and, CRP, and TNF-α levels were found to be significantly high in study group, whereas serum glucose IL-10 levels were significantly low comparing with control group. Positive correlation was observed between IL-6with glucose , CRP , and IL-8 , also between IL-10 with protein , TNF with IL-8 ,and IL-1 with CRP . whereas there was negative correlation between IL-6 with protein , TNF , IL-1 , IL-10 , also between IL-1 with IL-8 ,between IL-10 with, and TNF , IL-10 with glucose . More studies performed in suitable models of meningitis are required in order to establish the routine use of inflammatory markers in the diagnosis of infectious diseases of the central nervous system

scholarly journals Iron metabolism, cytokine secretion in patients with rheumatologic pathology

2021 ◽  
Vol 98 (9-10) ◽  
pp. 691-698
Author(s):  
V. T. Sakhin ◽  
E. V. Kryukov ◽  
M. A. Grigoryev ◽  
S. P. Kazakov ◽  
A. V. Sotnikov ◽  
...  
Keyword(s):  
Iron Metabolism ◽  
Saturation Index ◽  
Transferrin Saturation ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Reactive Protein ◽  
Tnf Α ◽  
Factor Alpha ◽  
Group 3 ◽  
Group 2

Aim. To study the eff ect of hepcidin, soluble transferrin receptor (sTfR ), and cytokines on iron metabolism and the development of anemia in rheumatologic patients, to propose a working version of the classifi cation of anemia of chronic diseases (ACD) according to the major nosotropic factor.Material and methods. 126 patients with rheumatic disease, 34 men (45.8 (36–54.9) years old), 92 women (49.5 (38–60) years old) were examined. Group 1 included 41 patients with ACD. Group 2 included 29 patients with the combination of ACD and IDA and 34 patients with iron defi ciency anemia (IDA). Group 3 included 34 patients with IDA and 29 — with the combination of ACD and IDA. Control group included 22 patients without anemia. Comparative analysis between groups with and without anemia and correlation analysis of hemogram parameters, iron metabolism, C-reactive protein (CRP), hepcidin, sTfR , interleukin-6 (IL-6), IL-1β, IL-10, interferon gamma (INF-γ) and tumor necrosis factor alpha (TNF-α) were performed.Results. In the ACD group, the concentrations of hepcidin, ferritin, CRP, IL-6 were increased in comparison with other groups. The correlation was revealed between erythrocytes, hemoglobin and IL-6 (r = −0.3 and −0.6), IL-10 (r = −0.4 and −0.4), INF-γ (r = −0.4 and −0.3), TNF-α (r = −0.3 and −0.3), hepcidin (r = −0.5 and −0.7), sTfR (r = −0.5 and −0.7). Dependence was shown between IL-6 and iron (r = –0.6), transferrin saturation index (TSI) (r = −0.5), ferritin (r = −0.5), CRP (r = 0.5), between TNF-α and TIBС (r = −0.6), transferrin (r = −0.6), ferritin (r = −0.7), between IL-1β and TIBC, ferritin, transferrin (r = −0.4). The correlation was noted between hepcidin and IL-6 (r = 0.5), IL-10 (r = 0.4), between sTfR and IL-6 (r = 0.4), IL-10 (r = 0.6), INF-γ (r = 0.4).Conclusion. The multicomponent genesis of anemia in patients with rheumatologic disease was detected. The signifi cance of disorders in iron metabolism, the eff ect of hepcidin, sTfR and cytokines on the development of anemia was found. A working version of ACD classifi cation (with a predominant iron defi ciency, with violations of the regulatory mechanisms of erythropoiesis, with insuffi cient production of erythropoietin) has been put forward.

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