scholarly journals Novel Strategies for Upstream and Downstream Processing of Tannin Acyl Hydrolase

2011 ◽  
Vol 2011 ◽  
pp. 1-20 ◽  
Author(s):  
Luis V. Rodríguez-Durán ◽  
Blanca Valdivia-Urdiales ◽  
Juan C. Contreras-Esquivel ◽  
Raúl Rodríguez-Herrera ◽  
Cristóbal N. Aguilar
Keyword(s):  
Gallic Acid ◽  
Downstream Processing ◽  
Industrial Applications ◽  
Culture Conditions ◽  
Negative Effects ◽  
Nonaqueous Media ◽  
Tannery Effluents ◽  
High Production ◽  
High Production Cost ◽  
Gallic Acid Esters

Tannin acyl hydrolase also referred as tannase is an enzyme with important applications in several science and technology fields. Due to its hydrolytic and synthetic properties, tannase could be used to reduce the negative effects of tannins in beverages, food, feed, and tannery effluents, for the production of gallic acid from tannin-rich materials, the elucidation of tannin structure, and the synthesis of gallic acid esters in nonaqueous media. However, industrial applications of tannase are still very limited due to its high production cost. Thus, there is a growing interest in the production, recovery, and purification of this enzyme. Recently, there have been published a number of papers on the improvement of upstream and downstream processing of the enzyme. These papers dealt with the search for new tannase producing microorganisms, the application of novel fermentation systems, optimization of culture conditions, the production of the enzyme by recombinant microorganism, and the design of efficient protocols for tannase recovery and purification. The present work reviews the state of the art of basic and biotechnological aspects of tannin acyl hydrolase, focusing on the recent advances in the upstream and downstream processing of the enzyme.

scholarly journals Fermentative Production of Mannosylerythritol Lipids using Sweetwater as Waste Substrate by Pseudozyma antarctica (MTCC 2706)

2021 ◽  
Vol 58 (4) ◽  
pp. 246-258
Author(s):  
Jayata Mawani ◽  
Jagruti Jadhav ◽  
Amit Pratap
Keyword(s):  
Soybean Oil ◽  
Industrial Applications ◽  
Commercial Production ◽  
Liquid Chromatography Mass Spectrometry ◽  
Mannosylerythritol Lipids ◽  
Pseudozyma Antarctica ◽  
Fermentative Production ◽  
Oil Concentration ◽  
High Production ◽  
High Production Cost

Abstract Mannosylerythritol lipids are glycolipid biosurfactants with promising industrial applications. However, their commercial production is hindered due to its high production cost. The current study investigates the use of sweetwater, a by-product of the fat-splitting industry in combination with soybean oil for the production of mannosylerythritol lipids using Pseudozyma antarctica (MTCC 2706). The optimum sweetwater and soybean oil concentration of 22% and 7% (w/v) yielded 7.52 g L–1and 21.5 g L–1 mannosylerythritol lipids at shake flask and fermenter level respectively. The structure and functional groups of mannosylerythritol lipids were confirmed by fourier transform infrared (FTIR) spectroscopy, liquid chromatography-mass spectrometry (LC/MS) and 1H- and 13C-nuclear magnetic resonance (NMR) analysis. Surfactant properties, such as surface tension, critical micelle concentration, foaming and emulsification of mannosylerythritol lipids were also explored.

scholarly journals Isolation of Polyhydroxybutyrate (PHB) Producing Bacteria, Optimization of Culture Conditions for PHB production, Extraction and Characterization of PHB

2019 ◽  
Vol 6 (1) ◽  
pp. 62-68 ◽  
Author(s):  
Christina Thapa ◽  
Pallavi Shakya ◽  
Rabina Shrestha ◽  
Sushovita Pal ◽  
Prakash Manandhar
Keyword(s):  
Culture Condition ◽  
Production Cost ◽  
Micrococcus Luteus ◽  
Culture Conditions ◽  
Bacillus Pasteurii ◽  
Nacl Concentration ◽  
Phb Production ◽  
High Production ◽  
High Production Cost ◽  
Micro Organisms

Polyhydroxybutyrates (PHBs) are energy reserves synthesized by different micro-organisms such as Alcaligenes, Pseudomonas, Staphylococcus, Algae, in excess of carbon and limitation of nutrients like nitrogen. These biopolymers are suitable alternate to synthetic carbon-based polymers. However, the high production cost limits their commercialization. The aim of this study was thus, focused on optimization of culture condition for maximum PHB production in an attempt to reduce the production cost. The micro-organisms for this purpose were isolated from 4 different soil samples and screened for PHB production. Culture conditions for these organisms were optimized by changing the parameters, viz., incubation time, pH, carbon source and NaCl concentration. Thus, optimized culture condition was used to culture the isolates for extraction of PHB and its analysis. The extracted compounds on FTIR-analysis gave characteristic C=O peak of PHB, thus, confirming the seven isolates to be PHB producers. Results for optimized parameters for the isolated PHB positive species showed that synthesis of PHB was maximum at 48 hours i.e. during the early stages of stationary phase. However, different isolates favored different culture conditions. Highest PHB accumulation and growth of isolates were seen at pH 7 and 9. Similarly, it was observed that glucose was favored by 4 isolates and sucrose was favored by 3 isolates. Interestingly, NaCl concentration did not cause significant effect on neither the bacterial growth nor the PHB production. During the extraction of PHB from the optimized culture conditions, extraction of PHB from broth gave significant yield than that from agar. A good PHB yield from broth amounting to 36.41% and 34.59% was observed for Bacillus pasteurii and Micrococcus luteus respectively, showing a potential for their exploitation in industrial PHB production. At optimized conditions, 7 isolates exhibited significant PHB yields, thus showing a potential for further exploitation.

Bio-based polyhydroxyalkanoates blends and composites

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Samy A. Madbouly
Keyword(s):  
Mechanical Properties ◽  
Functional Materials ◽  
Cost Effective ◽  
Production Method ◽  
Industrial Applications ◽  
Mechanical And Thermal Properties ◽  
Wide Range ◽  
High Production ◽  
High Production Cost ◽  
New Generation

Abstract Polyhydroxyalkanoates (PHAs) are linear semicrystalline polyesters produced naturally by a wide range of microorganisms for carbon and energy storage. PHAs can be used as replacements for petroleum-based polyethylene (PE) and polypropylene (PP) in many industrial applications due to their biodegradability, excellent barrier, mechanical, and thermal properties. The overall industrial applications of PHAs are still very limited due to the high production cost and high stiffness and brittleness. Therefore, new novel cost-effective production method must be considered for the new generation of PHAs. One approach is based on using different type feedstocks and biowastes including food byproducts and industrial and manufacturing wastes, can lead to more competitive and cost-effective PHAs products. Modification of PHAs with different function groups such as carboxylic, hydroxyl, amine, epoxy, etc. is also a relatively new approach to create new functional materials with different industrial applications. In addition, blending PHA with biodegradable materials such as polylactide (PLA), poly(ε-caprolactone) (PCL), starch, and distiller’s dried grains with solubles (DDGS) is another approach to address the drawbacks of PHAs and will be summarized in this chapter. A series of compatibilizers with different architectures were successfully synthesized and used to improve the compatibility and interfacial adhesion between PHAs and PCL. Finer morphology and significantly improvement in the mechanical properties of PHA/PCL blends were observed with a certain type of block compatibilizer. In addition, the improvement in the blend morphology and mechanical properties were found to be strongly influenced by the compatibilizer architecture.

A Short Review on Recent Development of Laccase Immobilization on Different Support Materials

2021 ◽  
Vol 02 (01) ◽  
Author(s):  
Norsyafiqah Amalina Ahmad Jafri ◽  
◽  
Roshanida A. Rahman ◽  
Noorhalieza Ali ◽  
◽  
...  
Keyword(s):  
Large Scale ◽  
Cost Effective ◽  
Short Review ◽  
Industrial Applications ◽  
Innovative Strategies ◽  
Support Materials ◽  
High Production ◽  
Efficient Recovery ◽  
High Production Cost ◽  
Laccase Immobilization

Laccase is a bio catalytic agent and multi-copper enzyme containing oxidases that are potentially great for oxidizing large number of phenolic and non-phenolic compounds. However, drawbacks do arise when laccase use in large scale; low in stability, high production cost, non-reusability, sensitive towards denaturing and poor storage ability of free enzymes. These problems lead to the progress in laccase immobilization in order to facilitate the efficient recovery and re-use of the enzyme, thus enabling cost-effective in continuous processes. Apart from discussing on different methods in laccase immobilization such as entrapment, encapsulation and cross-linking in general, we have reviewed a recent development in laccase immobilization on different supports or carriers binding (natural and synthetic). Future works are recommended to focus on innovative strategies on the modified supports to improve the enzyme immobilization as well as sensible entrapment techniques for industrial applications.

A feedback controller for milling chatter vibration control using a new response matrix

2021 ◽  
pp. 107754632098820
Author(s):  
Bashir B Muhammad ◽  
Muhammad Bashir ◽  
Mukhtar F Hamza ◽  
Mustapha Abdulhadi ◽  
Muhammad A Shehu ◽  
...  
Keyword(s):  
Controller Design ◽  
Industrial Process ◽  
Milling Process ◽  
Feedback Controller ◽  
Response Matrix ◽  
Chatter Vibration ◽  
Active Feedback ◽  
High Production ◽  
High Production Cost ◽  
Milling Chatter

A chatter mark is a result of irregular vibration that affects the milling process, which results in poor surface finish, reduced work quality, machine impairment, and high production cost. This work presents an active feedback controller design using a new response matrix to suppress the free vibration in the milling process. The proposed controller considers feed rate, tooth passing frequency, and time-varying dynamic milling force coefficients. A milling experiment verifies the effect of the proposed method. The method provides a reliable way of tackling chatter vibration in an industrial process. The procedure is technically and economically beneficial.

scholarly journals Preparation of Gallic Acid Esters and Their Antioxidant Properties for Biodiesel

2014 ◽  
Vol 26 (Supp.) ◽  
pp. S217-S221 ◽  
Author(s):  
Fashe Li ◽  
Ming Li ◽  
Guirong Bao ◽  
Hua Wang ◽  
Yizhe Li ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Antioxidant Properties ◽  
Gallic Acid Esters ◽  
Acid Esters

Phase Drift in Networks of Coupled Colpitts Oscillators

2021 ◽  
Vol 31 (04) ◽  
pp. 2130010
Author(s):  
Lourdes Coria ◽  
Horacio Lopez ◽  
Antonio Palacios ◽  
Visarath In ◽  
Patrick Longhini
Keyword(s):  
Collective Behavior ◽  
Traveling Wave ◽  
Scaling Law ◽  
Industrial Applications ◽  
Basins Of Attraction ◽  
Navigation Systems ◽  
Negative Effects ◽  
Phase Drift ◽  
Wide Range ◽  
Wave Patterns

In modern times, satellite-based global positioning and navigation systems, such as the GPS, include precise time-keeping devices, e.g. atomic clocks, which are crucial for navigation and for a wide range of economic and industrial applications. However, precise timing might not be available when the environment renders satellite equipment inoperable. In response to this critical need, we have been carrying out, over the past three years, theory and preliminary experiments [Buono et al., 2018a; Buono et al., 2018b; Palacios et al., 2020], towards developing a novel and inexpensive precision timing device that can function independently of GPS availability. The fundamental idea is to exploit collective behavior generated by networks of coupled nonlinear oscillators. Common sense may suggest that synchronized oscillations may lead to higher accuracy. Previous works show, however, that it is not synchronization but rather, traveling wave patterns, in which consecutive oscillators are out of phase by a constant amount, that can better reduce the negative effects of noise and material imperfections which cause phase drift. In this work we advance the state-of-art in the network-based concept by studying, mainly computationally, collective behavior in networks of Colpitts oscillators. These type of oscillators are chosen because they offer a wide range of advantages (such as the ability to tune up the oscillations over a broad frequency range). The results highlight the regions of parameter space, including coupling strength, where traveling wave patterns have the largest basins of attraction and the ability to reduce phase drift by a [Formula: see text] scaling law, where [Formula: see text] is the number of oscillators in the network. The results should also provide guidelines for follow-up design and fabrication tasks of a network-based technology for precision timing.

scholarly journals Thermal treatment of carbon fibre reinforced polymers (Part 1: Recycling)

2019 ◽  
Vol 37 (1_suppl) ◽  
pp. 73-82 ◽  
Author(s):  
Marco Limburg ◽  
Jan Stockschläder ◽  
Peter Quicker
Keyword(s):  
Thermal Treatment ◽  
Carbon Fibre ◽  
Polymer Matrix ◽  
Energy Recovery ◽  
State Of The Art ◽  
Reinforced Polymers ◽  
Carbon Fibre Reinforced Polymers ◽  
High Production ◽  
High Production Cost

The increasing use of carbon fibre reinforced polymers requires suitable disposing and recycling options, the latter being especially attractive due to the high production cost of the material. Reclaiming the fibres from their polymer matrix however is not without challenges. Pyrolysis leads to a decay of the polymer matrix but may also leave solid carbon residues on the fibre. These residues prevent fibre sizing and thereby reuse in new materials. In state of the art, these residues are removed via thermal treatment in oxygen containing atmospheres. This however may damage the fibre’s tensile strength. Within the scope of this work, carbon dioxide and water vapour were used to remove the carbon residues. This aims to eliminate or at least minimize fibre damage. Improved quality of reclaimed fibres can make fibre reuse more desirable by enabling the production of high-quality recycling products. Still, even under ideal recycling conditions the fibres will shorten with every new life-cycle due to production-based blending. Fibre disposal pathways will therefore always also be necessary. The problems of thermal fibre disintegration are summarized in the second part of this article (Part 2: Energy recovery).

scholarly journals Whole Conversion of Soybean Molasses into Isomaltulose and Ethanol by Combining Enzymatic Hydrolysis and Successive Selective Fermentations

Biomolecules ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 353
Author(s):  
Zhi-Peng Wang ◽  
Lin-Lin Zhang ◽  
Song Liu ◽  
Xiao-Yan Liu ◽  
Xin-Jun Yu
Keyword(s):  
Low Cost ◽  
Rhizomucor Miehei ◽  
Pichia Stipitis ◽  
Raffinose Family Oligosaccharides ◽  
Alternative Substrate ◽  
Soy Molasses ◽  
High Production ◽  
Novel Strategy ◽  
High Production Cost ◽  
High Sucrose

Isomaltulose is mainly produced from sucrose by microbial fermentation, when the utilization of sucrose contributes a high production cost. To achieve a low-cost isomaltulose production, soy molasses was introduced as an alternative substrate. Firstly, α-galactosidase gene from Rhizomucor miehei was expressed in Yarrowia lipolytica, which then showed a galactosidase activity of 121.6 U/mL. Under the effects of the recombinant α-galactosidase, most of the raffinose-family oligosaccharides in soy molasses were hydrolyzed into sucrose. Then the soy molasses hydrolysate with high sucrose content (22.04%, w/w) was supplemented into the medium, with an isomaltulose production of 209.4 g/L, and the yield of 0.95 g/g. Finally, by virtue of the bioremoval process using Pichia stipitis, sugar byproducts in broth were transformed into ethanol at the end of fermentation, thus resulting in high isomaltulose purity (97.8%). The bioprocess employed in this study provides a novel strategy for low-cost and efficient isomaltulose production from soybean molasses.

86 BIRTH OF HEALTHY CALVES AFTER INTRAFOLLICULAR OOCYTE TRANSFER

2015 ◽  
Vol 27 (1) ◽  
pp. 136
Author(s):  
M. Hoelker ◽  
A. Kassens ◽  
E. Held ◽  
C. Wrenzycki ◽  
U. Besenfelder ◽  
...  
Keyword(s):  
Lipid Content ◽  
Ex Vivo ◽  
Survival Rates ◽  
Blastocyst Stage ◽  
Culture Conditions ◽  
In Vitro Production ◽  
Negative Effects ◽  
Uterine Flushing

The in vitro production (IVP) of bovine embryos is a well-established technique that has been available for nearly 20 years. However, there remain major differences between IVP-derived blastocysts and their in vivo-derived counterparts. Many studies have pointed out that most of these differences are due to the in vitro developmental environment. To circumvent these negative effects due to in vitro culture conditions, a new method – intrafollicular oocyte transfer (IFOT) – was established in the present study. Using modified ovum pick-up (OPU) equipment, in vitro-matured oocytes derived from slaughterhouse ovaries were injected into the dominant preovulatory follicle of synchronised heifers (follicular recipients) enabling subsequent ovulation, in vivo fertilization, and in vivo development. A total of 810 in vitro-matured oocytes were transferred into 14 heifers. Subsequently, 222 embryos (27.3%) were recovered after uterine flushing at Day 7. Based on the number of cleaved embryonic stages, 64.2% developed to the blastocyst stage, which did not differ from the IVP-derived embryos (58.2%). Interestingly, lipid content of IFOT-derived blastocysts did not differ from the fully in vivo-produced embryos, whereas IVP-derived blastocysts showed significantly higher lipid droplet accumulation compared with fully in vivo-derived and IFOT-derived blastocysts (P < 0.05). Accordingly, IFOT blastocysts showed significantly higher survival rates after cryopreservation than complete IVP-derived embryos (77% v. 10%), which might be attributed to a lower degree of lipid accumulation. In agreement, transfer of frozen-thawed IFOT blastocysts to synchronized recipients (uterine recipients) resulted in much higher pregnancy rates compared with transfer of IVP-derived blastocysts (42.1 v. 13.8%) but did not differ from frozen-thawed ex vivo blastocysts (52.4%). Of these presumed IFOT pregnancies, 7 went to term, and microsatellite analysis confirmed that 5 calves were indeed derived from IFOT, whereas 2 were caused by fertilization of the follicular recipient's own oocyte after AI. Taken together, IFOT-derived blastocysts closely resemble in vivo-derived blastocysts, confirming earlier suggestions that the ability to develop to the blastocyst stage is already determined in the matured oocyte, whereas the quality in terms of lipid content and survival rate after cryopreservation is affected by the environment thereafter. However, to the best of our knowledge, this is the first study reporting healthy calves after intrafollicular transfer of in vitro-matured oocytes.

Sign in / Sign up

Export Citation Format

Share Document