scholarly journals Extraction and identification of microplastics from mussels: Method development and preliminary results

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Raffaelina Mercogliano ◽  
Serena Santonicola ◽  
Gennaro Raimo ◽  
Maurizio Gasperi ◽  
Giampaolo Colavita
Keyword(s):  
Preliminary Analysis ◽  
Method Development ◽  
Adriatic Sea ◽  
Aquatic Organisms ◽  
Fish Muscle ◽  
Extraction Yield ◽  
Environmental Risks ◽  
Feeding Strategies ◽  
Average Abundance ◽  
Recovery Percentage

Microplastics (MPs) are an emerging threat to marine ecosystems. One of the primary environmental risks is their bioavailability for aquatic organisms. Some fish and bivalves are of particular interest because their feeding strategies expose them to particles present in the water column. The aim of the study was to assess an extraction method in order to isolate and quantify MPs from fish gastrointestinal tract (n.8) and muscle (n.4), and bivalves (n.8) samples. The accuracy of the method was assessed through the calculation of the recovery percentage in samples spiked with a known number of MPs using microscopic observation. Successively, the extraction was preliminarily applied on n.20 mussels collected from mariculture plants of the Tyrrhenian and the Adriatic Sea. The results of the digestion protocol showed an average extraction yield of 80% in fish gastrointestinal tracts, 90% in fish muscle samples, and 95% in mussels. Preliminary analysis carried out on farmed mussels showed an average abundance of 3.8 items/individual, and 0.5 items/g of tissue, among those black, was the most represented color.

scholarly journals Studying sanitary and hygienic conditions of bream and water in its habitat

2021 ◽  
Vol 0 (4) ◽  
pp. 39-45
Author(s):  
Nuria Abdrakhimovna Kanieva ◽  
Elizaveta Aleksandrovna Stepanenko ◽  
Irina Volkova ◽  
Ksenia Popova
Keyword(s):  
Pathogenic Bacteria ◽  
Raw Materials ◽  
Aquatic Organisms ◽  
Fish Muscle ◽  
Microbiological Analysis ◽  
Abramis Brama ◽  
Mean Values ◽  
Anaerobic Microorganisms ◽  
Bream Abramis Brama ◽  
Study Results

The article highlights the data on microbiological monitoring of fish raw materials and water of the Volga-Caspian fishing channel in 2020-2021. The most indicative organisms for assessing the quality of the aquatic environment are benthos-eating fish. Bream Abramis brama (Linnaeus, 1758) is one of the valuable objects of fishing in the Astrakhan region. As a commercial object of river coastal fishing, bream has a higher bacterial contamination than fish caught in the sea. In this regard, studying the conditions for bacteriocenosis of fish raw materials, as well as the factors that determine the pathogenicity of its microflora and sanitary and epizootic monitoring by the microbiological tests remain the most relevant research of aquatic organisms. Quantitative and qualitative indicators of the bream microflora were estimated, according to the analysis of 180 species. The indicators of the water microflora were analyzed using the results of studies of 90 samples. The species identification of the isolated microflora was carried out according to the Bergey identification keys. In the samples of bream muscles there was determined the total number of facultative anaerobic microorganisms and aerobic mesophylls, presence of coliform microorganisms, as well as a causative agent of Staphylococcus aureus, pathogenic bacteria Salmonella and Listeria, and parahemolytic vibrios Vibrio. The study results were processed, according to the methods of biological statistics in the Microsoft Excel 2010 software. The results are presented as mean values and standard errors (M ± m). The general microbiological analysis of the fish muscle tissue confirmed the satisfactory sanitary state of bream Abramis brama (L., 1758) caught in the fishing area of the Volga-Caspian Canal during the study period. Pathogenic microflora in the form of E. coli bacteria, bacteria Salmonella, L. monocytogenes and St. aureus were not found

scholarly journals The Effect of Neonicotinoid Insecticides on the Structure and Stability of Bio-Macromolecules

2021 ◽  
Author(s):  
Valéria Verebová ◽  
Jana Staničová
Keyword(s):  
High Risk ◽  
Aquatic Organisms ◽  
Environmental Risks ◽  
Biological Macromolecules ◽  
Serum Albumins ◽  
Biological Origin ◽  
Structure And Stability ◽  
The World ◽  
The Impact ◽  
Very High

Insecticides are among the most widely used pesticides in the world. They are preparations of chemical and biological origin used to control insects, which means its killing or preventing its destructive activity. Majority are used in forestry, agriculture, and households. Neonicotinoids represent the class of insecticides that is most frequently used in the world and replaced by more dangerous pyrethroids, organophosphates, and carbamates. In recent years, the focus has been mainly on the ecological and environmental risks caused by the use of neonicotinoids. These insecticides pose a very high risk to bees and also to soil and aquatic organisms. It is therefore highly topical to address the impact of neonicotinoids on biological systems on individual bio-macromolecules (DNA and serum albumins). Monitoring the impact of neonicotinoids on the structure and stability of biological macromolecules may contribute to reducing the use of these insecticides, as well as to considering and adjusting the tolerances of insecticides and their residues in food.

scholarly journals Microplastics in Surface Sediments along the Montenegrin Coast, Adriatic Sea: Types, Occurrence, and Distribution

2021 ◽  
Vol 9 (8) ◽  
pp. 841
Author(s):  
Neda Bošković ◽  
Danijela Joksimović ◽  
Milica Peković ◽  
Ana Perošević-Bajčeta ◽  
Oliver Bajt
Keyword(s):  
Fourier Transform ◽  
Surface Sediment ◽  
Surface Sediments ◽  
Adriatic Sea ◽  
Marine Debris ◽  
Acrylate Copolymer ◽  
Synthetic Materials ◽  
Sampling Locations ◽  
Average Abundance ◽  
Common Shape

Considering that microplastics are widespread in the marine environment, in this study we evaluated the presence, identify distribution, abundance, shape type, and color of microplastics in surface sediment along the Montenegrin coast, on the Adriatic Sea. These preliminary results provide the first published record of microplastics found in the surface sediment of this area and highlight the importance of microplastics as a component of marine debris. We documented the presence of microplastics at all sampling locations. The identification of polymer types was performed using Fourier-transform infrared (FTIR) spectroscopy, whereby the presence of three polymer types became evident: polypropylene (54.5%), polyethylene (9.7%), and acrylate copolymer (2.0%). Another 22.2% of particles were unidentified polymers, and the remaining 11.5% were non-synthetic materials. The most common shape type of microplastics was filaments (55.5%), followed by granules (26.3%), fragments (14.9%), and films (3.3%). The dominant colors of microplastics followed the order: blue > yellow > red > clear > black > green > blue-white > white. The average abundance of microplastics in all sampling locations was 609 pieces of microplastic/kg of dry sediment. Compared with other studies, the surface sediment of the Montenegrin coast is moderately to highly polluted with microplastics, depending on the examined location.

The environmental risks of pharmaceuticals beyond traditional toxic effects: Chemical differences that can repel or entrap aquatic organisms

2021 ◽  
Vol 268 ◽  
pp. 115902
Author(s):  
Raquel Sampaio Jacob ◽  
Cristiano V.M. Araújo ◽  
Lucilaine Valéria de Souza Santos ◽  
Victor Rezende Moreira ◽  
Yuri Abner Rocha Lebron ◽  
...  
Keyword(s):  
Aquatic Organisms ◽  
Environmental Risks ◽  
Toxic Effects

scholarly journals Effect of Sublethal Concentrations of Cadmium on the Histo-pathological Changes of Muscles of Planiliza abu Juveniles (Heckel, 1843)

2020 ◽  
Vol 33 (2) ◽  
pp. 207-217
Author(s):  
Entesar SH. Hashim ◽  
Rajaa N. Al-Yassein
Keyword(s):  
Morphological Changes ◽  
Aquatic Organisms ◽  
Fish Muscle ◽  
Low Doses ◽  
Muscle Bundle ◽  
Cd Toxicity ◽  
Muscle Tissues ◽  
International Issue ◽  
Detrimental Impact ◽  
Sublethal Concentrations

Water heavy metals contamination is an international issue due to its detrimental impact on the environment, aquatic organisms and impairment of the ecological balance systems. Cadmium is a heavy metal that is extremely toxic, even in low doses, thus it is a big concern to the ecosystem. Cd can cause several Patho-morphological changes in various organs in fish. This study was evaluated the effect of sublethal concentrations of Cd toxicity and effects on Histopathological of muscle tissues of Planiliza abu Juvenile. Series of Cd concentrations (0.5- 2) ppm were used and the 15d LC50 was 0.4 ppm. Several histopathological alterations were observed which increase gradually with increased concentration, included, marked thickening and hypertrophy of muscle bundle were will observed  in lowest concentration (0.5 ppm). While in the highest concentration (2 ppm),  atrophy, splitting, and separation with intracellular edema were marked  in fish muscle bundle.

scholarly journals KELIMPAHAN DAN KEANEKARAGAMAN GASTROPODA PADA ZONA INTERTIDAL DI PULAU BANGKA BAGIAN TIMUR

2018 ◽  
Vol 3 (1) ◽  
pp. 10-21 ◽  
Author(s):  
Okto Supratman ◽  
Arthur Muhammad Farhaby ◽  
Jemi Ferizal
Keyword(s):  
Water Quality ◽  
Resource Management ◽  
Data Collection ◽  
Intertidal Zone ◽  
Water Resource Management ◽  
Diversity Index ◽  
Aquatic Organisms ◽  
Anthropogenic Activity ◽  
Mining Activities ◽  
Average Abundance

 AbstractEnvironmental damage from anthropogenic activity will lead to a decrease in water quality, which then affects aquatic organisms including Gastropoda. So it is necessary to do a study of diversity and abundance of gastropods in East Bangka Island.The study was conducted from June to December 2017. The research locations were divided into 6 stations. Data collection of gastropods using squares of size 50 cm x 50 cm, performed by direct gastropod removal by hand, then identified in Water Resource Management Laboratory. The results of this study found 12 species of gastropods from 8 families. The families found are Cerithideidae (2 species), Cerithiidae (1 species), Cirridae (1 species), Nassariidae (2 species), Neritidae (2 species), Planaxidae (1 species), Muricidae (1 Species) and Costellariidae (2 Species ). The highest abundance of gastropods is Certhidea cingulata species with an average abundance of 124.54 ind / m2. The value of diversity index of gastropod in the eastern part of the island of Bangka categorized small, it is thought to be caused by loss of habitat as a result of mining activities at sea. Keywords: Gastropods, Diversity, Abundance, Intertidal Zone

scholarly journals Concentration of Metals in Native and Invasive Species of Fish in the Fluvial-Lagoon-Deltaic System of the Palizada River, Campeche

Fishes ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 72
Author(s):  
María del Refugio Castañeda-Chávez ◽  
Fabiola Lango-Reynoso ◽  
Gabycarmen Navarrete-Rodríguez ◽  
Armando Toyokazu Wakida-Kusunoki
Keyword(s):  
Muscle Tissue ◽  
Fish Species ◽  
Biological Diversity ◽  
Sampling Site ◽  
Aquatic Organisms ◽  
Dry Weight ◽  
Fish Muscle ◽  
Invasive Fish ◽  
Significant Difference ◽  
Microwave System

Aquatic organisms, such as fish, are important indicators of the bioavailability of metals in coastal environments, demonstrating the capacity of different species to bioaccumulate these metallic elements. The fluvial-lagoon system of the Palizada River is an important ecosystem for its terrestrial and aquatic biological diversity where fishing is an important productive activity in this system. The objective of this research was to evaluate the concentration of Pb and Cd in the muscle tissue of native and invasive fish species in this area. For this, the digestion of fish muscle samples was carried out with a CEM MARS 5 Digestion Microwave System, while the quantification of metals was performed with an atomic absorption equipment. All analysis muscle samples from native and invasive fish showed Pb and Cd in their content. By sampling site there was a statistically significant difference (p < 0.05), with a maximum Pb concentration of 7.760 µg g−1 at the LLAR site (Laguna Larga). In terms of species, the maximum Cd concentration was obtained in the Cyprinus carpio with 6.630 ± 0.127 and in Pterygoplichthus pardalis with 6.547 ± 0.873 µg g−1 (dry weight). The presence of metals such as Pb and Cd in muscle tissue of native and invasive fish species represents an important bioindicator of environmental exposure in the study area and a potential risk to public health, as these species are commonly consumed.

A review of microplastic pollution in commercial fish for human consumption

2022 ◽  
Vol 0 (0) ◽  
Author(s):  
Pouran Makhdoumi ◽  
Hooshyar Hossini ◽  
Meghdad Pirsaheb
Keyword(s):  
Health Hazard ◽  
Daily Intake ◽  
Aquatic Organisms ◽  
Fish Muscle ◽  
Human Consumption ◽  
Human Populations ◽  
Commercial Fish ◽  
Environmental Threat ◽  
Global Environmental ◽  
Fish Samples

Abstract Today microplastics (MPs) have received worldwide attention as an emerging environmental pollution which is one of the four major global environmental threat and health hazard to human as well. Unfortunately, MPs have been founded in the all environments and media include air, water resources, sediments, and soil. It should not be forgotten MPs have also been detected in food and processing products like tuna. MPs can be ingested by marine organisms such as zooplankton, fish and birds. Accumulation and distribution of MPs by commercially important aquatic organisms is expected to lead to greater exposure risk for human populations with possible adverse effects over time. The aim of this work was to review the published literature regarding the contamination of commercial fish muscle for human consumption. Furthermore, a short revision of the environmental contamination and human health effects by MPs are included. We also estimated human daily intake considering the worldwide contamination of commercial fish muscle ranged from 0.016 items/g muscle of fish to 6.06 items/g muscle of fish. MPs have been found in 56.5% of the commercial fish samples analysed here. As fish is used in human food table across the word, they constitute a long-term exposure route for all humans and raise the concern about the potential public health risk.

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