scholarly journals The Effect of Moringa Oleifera Methanol Leaves Extracts on Sodium/Potassium (Na+ /K+ ) Atpase in Streptozotocin-Induced Experimental Diabetic Albino Male Rat Models

2021 ◽  
Vol 16 (2) ◽  
pp. 159-165
Author(s):  
E.M. Alozie
Keyword(s):  
Blood Glucose ◽  
Moringa Oleifera ◽  
Glycosylated Hemoglobin ◽  
Fasting Blood Glucose ◽  
Male Rats ◽  
Male Rat ◽  
Phytochemical Analysis ◽  
Sodium Potassium ◽  
Glucose Levels ◽  
Potassium Atpase

Background: An evaluation of the effect of Moringa oleifera methanol leaves extracts on Sodium/Potassium ATPase  xin streptozotocin-induced experimental diabetic albino male rats’ model.Methods: Q ualitative phytochemical analysis was carried out on the methanol extracts of M. oleifera leaves usingacceptable chemical methods. The LD50 of the plant extract was conducted and was non-lethal at 5000mg/kg. Thirty, albino male rats weighing 120g – 180g were arranged into five groups, comprising six rats per group. Parameters such as sodium/potassium ATPase (Na+/K+ ATPase), glycosylated hemoglobin (HbA1c), and fasting blood glucose levels were assayed.Results: M.oleifera methanol leaves extract (at 500mg/kg and 1000mg/kg) increased sodium/potassium -ATPase activities in an albino male rat induced with hyperglycemia. It was also observed that the extract at doses of 500mg/kg (132.67 + 8.14) and1000mg/kg (114.00 + 15.38) for fasting blood glucose, 500mg/kg (6.29 + 0.26) and1000mg/kg (6.08 + 0.26) for glycosylated haemoglobin (HbA1c) were effective in ameliorating diabetes induced bystreptozotocin.Conclusions: This study established that the decrease in the activity of sodium/potassium -ATPase in streptozotocininduced type II diabetes in albino male rats can be increased to normal functionality by oral administration of M. oleifera methanol leaves extracts at doses of 500mg/kg and 1000mg/kg.

scholarly journals Markers of glycemic control in the mouse: comparisons of 6-h- and overnight-fasted blood glucoses to Hb A1c

2008 ◽  
Vol 295 (4) ◽  
pp. E981-E986 ◽  
Author(s):  
Byoung Geun Han ◽  
Chuan-Ming Hao ◽  
Elena E. Tchekneva ◽  
Ying-Ying Wang ◽  
Chieh Allen Lee ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glycemic Control ◽  
Glycosylated Hemoglobin ◽  
Fasting Blood Glucose ◽  
Glucose Monitoring ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Overnight Fasting ◽  
Highly Correlated ◽  
Method R

The present studies examined the relationship between fasting blood glucose and Hb A1cin C57BL/6J, DBA/2J, and KK/HlJ mice with and without diabetes mellitus. Daily averaged blood glucose levels based on continuous glucose monitoring and effects of 6-h vs. overnight fasting on blood glucose were determined. Daily averaged blood glucose levels were highly correlated with Hb A1c, as determined with a hand-held automated device using an immunodetection method. R2values were 0.90, 0.95, and 0.99 in KK/HIJ, C57BL/6J, and DBA/2J, respectively. Six-hour fasting blood glucose correlated more closely with the level of daily averaged blood glucose and with Hb A1cthan did blood glucose following an overnight fast. To validate the immunoassay-determined Hb A1c, we also measured total glycosylated hemoglobin using boronate HPLC. Hb A1cvalues correlated well with total glycosylated hemoglobin in all three strains but were relatively lower than total glycosylated hemoglobin in diabetic DBA/2J mice. These results show that 6-h fasting glucose provides a superior index of glycemic control and correlates more closely with Hb A1cthan overnight-fasted blood glucose in these strains of mice.

scholarly journals Pengaruh Vitamin D3 Terhadap Glukosa Darah Tikus Wistar Yang Dipapar Asap Rokok

2020 ◽  
Vol 1 (2) ◽  
pp. 130-138
Author(s):  
Lolita Putri Nanda Utami ◽  
Hetti Rusmini ◽  
Yessi Nurmalasari ◽  
Dessy Hermawan
Keyword(s):  
Blood Glucose ◽  
Cigarette Smoke ◽  
Vitamin D3 ◽  
Rattus Norvegicus ◽  
Fasting Blood Glucose ◽  
Male Rats ◽  
Control Group ◽  
Not Given ◽  
Glucose Levels ◽  
Blood Glucose Levels

Cigarettes are the biggest health problem around the world. Nicotine in cigarettes has been proved insulin receptor resistance and can reduce insulin secretion in pancreatic β cells, which causes an increase in blood glucose levels. One of the efforts to reduce blood glucose levels is to consume vitamin D3. Vitamin D3 is a prohormone that plays a role in metabolic functions in cells and repair functions of cells. Purpose: to know the effect of vitamin D3 toward glucose blood level of male Rattus norvegicus Wistar exposed by cigarette smoke. Methods: This research conducted with pure experimental pre- and post-test with control group design. The samples used were 24 male rats. The sample divided into four groups, including K1, K2, K3, and P1. K1, which is not exposed by cigarette smoke and not given of vitamin D3. K2 is a group exposed by cigarette smoke but not given vitamin D3. K3 is a group that is not exposed by cigarette smoke but given vitamin D3 a dose of 0.2 µgr /head. P1 is a group exposed by cigarette smoke and given a vitamin D3 dose of 0.2 µgr /head. Results: Paired T-test showed increased blood glucose levels that are meaningful (p<0,05) In groups K2 (p=0,018) and P1 (p=0,035). One-way Anova showed there were differences in blood glucose levels that are meaningful between groups (p=0,001). Statistical analysis Post Hoc LSD showed significant differences in group K1 with K2 (p=0,004), group K1 with K3 (p=0,038), group K1 with P1  (p=0,038), group K2 with K3 (p=0,000),  group K2 with P1  (p=0,004). Conclusion: Vitamin D3 can reduce fasting blood glucose levels of Rattus norvegicus exposed by cigarette smoke.

scholarly journals Effect of atypical antipsychotics on blood glucose levels and HbA1c in patients of schizophrenia and bipolar disorder.

2021 ◽  
Vol 28 (02) ◽  
pp. 158-164
Author(s):  
Abdul Hafeez Bughio ◽  
Shafak Ansari ◽  
Rajesh Kumar ◽  
Muhammad Hassan Sheikh ◽  
Tarachand Devrajani
Keyword(s):  
Diabetes Mellitus ◽  
Bipolar Disorder ◽  
Blood Glucose ◽  
Atypical Antipsychotics ◽  
Glycosylated Hemoglobin ◽  
Fasting Blood Glucose ◽  
Bipolar Disorders ◽  
Antipsychotic Agents ◽  
Antipsychotic Therapy ◽  
Glucose Levels

Objectives: To evaluate the effect of atypical antipsychotics on serum glucose levels and HbA1C in patients of schizophrenia and bipolar disorder. Study Design: Quasi-experimental study. Setting: Department of Neurology and Sir C. J Institute of Psychiatry LUMHS Jamshoro/Hyderabad. Period: Dec 2018-Dec 2019. Material & Methods: Total 360 participants of age more than 15 years of either gender presenting with psychiatric illness i.e. schizophrenia and bipolar disorder and prescribed same brand of antipsychotic drugs were included in the study. Fasting blood glucose (FBS), random blood glucose (RBS) and glycosylated hemoglobin (HbA1c) were measured at baseline and 6th months after treatment with atypical antipsychotic agents. SPSS version 23 was used to analyze data. Results: A total of 360 patients were enrolled in the study duration, among them 338 patients were followed up till 6 months, while 22 patients were lost to follow up. The mean age of the study sample was reported as 39.33±8.83 years. At baseline mean FBS, RBS and HbA1c were reported as 92.52±9 mg/dl, 143.21±14.91, 5.83±0.37 which significantly increase after treatment with antipsychotics at 6 months (p<0.05). About 23.6% developed diabetes mellitus and 21.3% developed hyperglycemia at the end of 6 months. Conclusion: Non-diabetic treatment naïve schizophrenia and bipolar disorders patients have higher chances developing side-effects on the glucose regulations after initiation of antipsychotic therapy. Overall, the early identification and diagnosis of antipsychotic-induced diabetes mellitus and hyperglycemia requires proper evaluation, reporting, and physician and patient awareness.

scholarly journals TEST OF THE EFFECTIVENESS ANTIHIPERGLIKEMIA OF COMBINATION CUCUMBER JUICE(Cucumis sativus) AND TOMATO (Solanum lycopersicum L) ON THE WISTAR MALE RAT

2017 ◽  
Vol 4 (1) ◽  
pp. 16-23
Author(s):  
Suntoro ◽  
Ressi Susanti ◽  
Robiyanto
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Tomato Juice ◽  
Male Rat ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Significant Difference ◽  
Decrease Blood Glucose ◽  
Tolerance Method

Diabetes mellitus is a metabolic disease with characteristics of hyperglycemia. Conventional treatments using oral medications such as glibenclamide and metformin have many side effects. Therefore, it is necessary to find an alternative drug that is more effective and safer. Some agents that are often used as anantihiperglikemia are cucumber and tomato.The purpose of this study was to determine the effectiveness of combination of cucumber and tomato juice to decrease blood glucose levels of male Wistar ratsusingglucose tolerance method. Male Wistar rats were fasted for 10 hours and then measured the levels of fasting blood glucose. Then animals test were given the juice (single and combination), 30 minutes later were given a dose of sucrose load of 6.75 g / KgBW orally. Blood glucose levels of animal testre-measured at 30th, 60th, 90th, and 120th minutes after loading sucrose using a glucometer.The measurement results showed that blood glucose levels of the combination of cucumber and tomato juice group with dose ratio of 28 g/KgBW:16.8 g/KgBW gave decrease blood glucose levels significantly compared to the negative control group (p≤0.05) and didn’t not provide a significant difference compared to the metformin group (p ≥0.05).

scholarly journals Is There A Way to Prevent Aging? A Study Using Metformin in Blood Sugar Levels and Serum Levels of IGF-1

2021 ◽  
Vol 13 (1) ◽  
pp. 51
Author(s):  
Arlina Wiyata Gama ◽  
Arief Santoso ◽  
Ika Yustisia ◽  
Nurpudji Astuti Taslim ◽  
Irfan Idris ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Calorie Restriction ◽  
Fasting Blood Glucose ◽  
White Male ◽  
Serum Levels ◽  
Male Rats ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reduce Blood Glucose

Aging is influenced by lifestyle, which one is by consuming excessive calories. In recent years there have been many studies on aging and age-related diseases, calorie restriction mimetic is one of them. Metformin is a calorie restriction mimetic that is attractive to gerontologists. This research analyzed the effect of metformin as a calorie restriction mimetic on fasting blood glucose and IGF-1 serum levels in old white male Wistar rats. This study conducted with an experimental study with a pre and post-test pattern with controlled group design on male rats (Rattus novergicus) Wistar strain which was divided into 3 groups, control group (K), the calorie restriction group (P1), and the metformin group (P2), 6 rats each group. Blood glucose levels were measured by a glucometer and serum IGF-1 levels were measured with an ELISA kit, where blood samples were taken from the tail of the rats. In general, the provision of metformin and calorie restriction tended to reduce blood glucose levels, but increased serum IGF-1 levels. There is a significant relationship between blood glucose levels and serum IGF-1 levels. It’s necessary to conduct further research to determine the effective dose and maximum dose of metformin to reduce blood glucose level and serum IG-1 levels which can be slow down the aging process.

Detection of Glucose in Human Serum Based on Silicon Dot Probe

2020 ◽  
Vol 16 (6) ◽  
pp. 744-752
Author(s):  
Kuan Luo ◽  
Xinyu Jiang
Keyword(s):  
Quantum Dots ◽  
Blood Glucose ◽  
Human Serum ◽  
Organic Dyes ◽  
Limit Of Detection ◽  
Fasting Blood Glucose ◽  
Glucose Biosensor ◽  
Metal Nanoclusters ◽  
Glucose Levels ◽  
Highly Sensitive

Background: Diabetes Mellitus (DM) is a major public metabolic disease that influences 366 million people in the world in 2011, and this number is predicted to rise to 552 million in 2030. DM is clinically diagnosed by a fasting blood glucose that is equal or greater than 7 mM. Therefore, the development of effective glucose biosensor has attracted extensive attention worldwide. Fluorescence- based strategies have sparked tremendous interest due to their rapid response, facile operation, and excellent sensitivity. Many fluorescent compounds have been employed for precise analysis of glucose, including quantum dots, noble metal nanoclusters, up-converting nanoparticles, organic dyes, and composite fluorescent microspheres. Silicon dot as promising quantum dots materials have received extensive attention, owing to their distinct advantages such as biocompatibility, low toxicity and high photostability. Methods: MnO2 nanosheets on the Si nanoparticles (NPs) surface serve as a quencher. Si NPs fluorescence can make a recovery by the addition of H2O2, which can reduce MnO2 to Mn2+, and the glucose can thus be monitored based on the enzymatic conversion of glucose by glucose oxidase to generate H2O2. Therefore, the glucose concentration can be derived by recording the fluorescence recovery spectra of the Si NPs. Results: This probe enabled selective detection of glucose with a linear range of 1-100 μg/mL and a limit of detection of 0.98 μg/mL. Compared with the commercial glucometer, this method showed favorable results and convincing reliability. Conclusion: We have developed a novel method based on MnO2 -nanosheet-modified Si NPs for rapid monitoring of blood glucose levels. By combining the highly sensitive H2O2/MnO2 reaction with the excellent photostability of Si NPs, a highly sensitive, selective, and cost-efficient sensing approach for glucose detection has been designed and applied to monitor glucose levels in human serum with satisfactory results.

scholarly journals Association of a Self-Paid Medically Supervised Weight Management Program with Reversal of Obesity-Associated Impaired Fasting Glucose

2021 ◽  
Vol 11 (2) ◽  
pp. 386-394
Author(s):  
Vijaya Surampudi ◽  
Xinkai Zhou ◽  
Chi-Hong Tseng ◽  
David Heber ◽  
Zhaoping Li
Keyword(s):  
Weight Loss ◽  
Blood Glucose ◽  
Retrospective Analysis ◽  
Weight Management ◽  
Impaired Fasting Glucose ◽  
Fasting Glucose ◽  
Fasting Blood Glucose ◽  
Management Program ◽  
Weight Management Program ◽  
Glucose Levels

Aims: The progression of prediabetes to T2DM can be delayed through diet modification and weight management. However, the intensive lifestyle program is often not covered by medical insurance. This retrospective analysis evaluates the association of a patient self-paid weight management program on an improvement of blood sugar in overweight and obese patients with impaired fasting glucose (IFG). Methods: The medical records of 4634 patients who participated in the self-pay UCLA Weight Management Program were reviewed and 2572 patients met the criteria for this retrospective analysis to examine whether this program was associated with the reversal of IFG over 3 months among 1396 patients with normal fasting glucose (NFG) and 1176 with IFG. Results: The patients with IFG lost comparable amounts of weight (10.5 ± 1.3 kg) at three months, as did the subjects with NFG (10.1 ± 1.3 kg). Fasting blood glucose in the IFG group decreased from 108.49 ± 6.4 to 101.8 ± 9.41 mg/dL (p < 0.0001) after three months. There were also significant reductions in triglycerides, and both systolic and diastolic blood pressure in both groups in association with weight loss. Conclusion: Our medically supervised self-pay multidisciplinary weight management program was associated with reduced fasting blood glucose levels in patients with IFG over three months with comparable weight loss to patients with NFG.

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