Pengaruh Vitamin D3 Terhadap Glukosa Darah Tikus Wistar  Yang Dipapar Asap Rokok

Cigarettes are the biggest health problem around the world. Nicotine in cigarettes has been proved insulin receptor resistance and can reduce insulin secretion in pancreatic β cells, which causes an increase in blood glucose levels. One of the efforts to reduce blood glucose levels is to consume vitamin D3. Vitamin D3 is a prohormone that plays a role in metabolic functions in cells and repair functions of cells. Purpose: to know the effect of vitamin D3 toward glucose blood level of male Rattus norvegicus Wistar exposed by cigarette smoke. Methods: This research conducted with pure experimental pre- and post-test with control group design. The samples used were 24 male rats. The sample divided into four groups, including K1, K2, K3, and P1. K1, which is not exposed by cigarette smoke and not given of vitamin D3. K2 is a group exposed by cigarette smoke but not given vitamin D3. K3 is a group that is not exposed by cigarette smoke but given vitamin D3 a dose of 0.2 µgr /head. P1 is a group exposed by cigarette smoke and given a vitamin D3 dose of 0.2 µgr /head. Results: Paired T-test showed increased blood glucose levels that are meaningful (p<0,05) In groups K2 (p=0,018) and P1 (p=0,035). One-way Anova showed there were differences in blood glucose levels that are meaningful between groups (p=0,001). Statistical analysis Post Hoc LSD showed significant differences in group K1 with K2 (p=0,004), group K1 with K3 (p=0,038), group K1 with P1 (p=0,038), group K2 with K3 (p=0,000), group K2 with P1 (p=0,004). Conclusion: Vitamin D3 can reduce fasting blood glucose levels of Rattus norvegicus exposed by cigarette smoke.

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UJI ANTIDIABETIK EKSTRAK ETANOL BUNGA PEPAYA (Carica papaya L.) TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽

10.35799/pha.9.2020.27423 ◽

2020 ◽

Vol 9 (1) ◽

pp. 160

Author(s):

Adinda Fransisca Pongoh ◽

Edwin De Queljoe ◽

Henki Rotinsulu

Keyword(s):

Blood Glucose ◽

Rattus Norvegicus ◽

Carica Papaya ◽

Fasting Blood Glucose ◽

Ethanol Extract ◽

Control Group ◽

Homogeneity Test ◽

Glucose Levels ◽

White Rats ◽

Blood Glucose Levels

ABSTRACT This study aims to determine the antidiabetic activity of papaya flower ethanol extract (Carica papaya L.) against male white rats (Rattus norvegicus) induced by alloxan. This research is experimental. Fifteen rats were divided into 5 treatment groups, each group consisted of 3 rats. The first group was the negative control group given Aquades, the second group was positive control given Glibenklamid, the three groups were 200 mg dose variation groups, four groups were 400 mg dose variations, and the five groups were 800 mg dose variations. Previously, rats were examined fasting blood glucose levels, then mice were induced by an alloxan dose of 120 mg / kgBW intraperitoneally. On the 3rd day blood glucose levels were examined and then treated according to groups for 7 days, measurement of blood glucose levels after the treatment was carried out once every 3 days namely day 3, day 7, and day 10. Data obtained were then analyzed statistically using SPSS , including normality test (Shapiro-Wilk), homogeneity test (Levene), ANOVA test (One way). The results of this study indicate that Papaya Flower (Carica papaya L.) Ethanol Extract can provide the best blood glucose level reduction effect at a dose of 800 mg. Keywords : Antidiabetic, Papaya Flower Extract (Carica papaya L.), Diabettes Mellitus, Male White Rat (Rattus norvegicus), Aloxan. ABSTRAK Penelitian ini bertujuan untuk mengetahui efektivitas Antidiabetik Ekstrak Etanol Bunga Pepaya (Carica papaya L.) Terhadap Tikus Putih Jantan (Rattus norvegicus) Yang Diinduksi Aloksan. Penelitian ini bersifat eksperimental. Sebanyak 15 ekor tikus dibagi ke dalam 5 kelompok perlakuan masing- masing kelompok terdiri dari 3 ekor tikus. Kelompok pertama kelompok Kontrol negatif yang diberikan Aquades, Kelompok kedua Kontrol Positif yang diberikan Glibenklamid, kelompok tiga kelompok variasi dosis 200 mg, kelompok empat kelompok variasi dosis 400 mg, dan kelompok lima kelompok variasi dosis 800 mg. Sebelumnya tikus dilakukan pemeriksaan kadar glukosa darah puasa, selanjutnya tikus diinduksi Aloksan dosis 120 mg/kgBB secara intraperitoneal. Pada hari ke 3 diperiksa kadar Glukosa darah kemudian diberikan perlakuan sesuai kelompok selama 7 hari, pengukuran kadar glukosa darah setelah perlakuan dilakukan 3 hari sekali yaitu hari ke 3, hari ke 7, dan hari ke 10. Data yang diperoleh kemudian dianalisis secara statistik menggunakan SPSS, meliputi uji normalitas (Shapiro-Wilk), uji homogenitas (Levene), uji ANOVA (One way). Hasil penelitian ini menunjukkan bahwa Ekstrak Etanol Bunga Pepaya (Carica papaya L.) dapat memberikan efek penurunan kadar glukosa darah yang paling baik yaitu pada dosis 800 mg. Kata kunci : Antidiabetik, Ekstrak Bunga pepaya (Carica papaya L.), Diabettes Mellitus, TikusPutih Jantan (Rattus norvegicus), Aloksan.

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Effect of Celery Extract on Fructose Induced Insulin Resistance Rattus norvegicus

Folia Medica Indonesiana ◽

10.20473/fmi.v56i4.23411 ◽

2020 ◽

Vol 56 (4) ◽

pp. 269

Author(s):

Devitya Angielevi Sukarno ◽

Arifa Mustika ◽

Purwo Sri Rejeki

Keyword(s):

Insulin Resistance ◽

Blood Glucose ◽

Treatment Group ◽

Rattus Norvegicus ◽

Therapy Group ◽

Fasting Blood Glucose ◽

Apium Graveolens ◽

Control Group ◽

Glucose Levels ◽

Blood Glucose Levels

Diabetes mellitus (DM) is one of the global health problems and in the top 4th ranks as the main cause of death in developing countries (IDF, 2015). The pathogenesis of type II DM involves abnormalities in insulin secretion and activity that leads to insulin resistance. This research aims to study the efficacy of celery (Apium graveolens) as a prevention of insulin resistance. In this study, the samples were 45 Wistar rats (Rattus norvegicus), male sex, aged 4-6 weeks, weight 150-175 grams, and had normal fasting blood glucose levels by tested before treatment. The experimental animals were divided into 5 groups, K1 was negative control group (insulin resistance by given 20% fructose 1,86 g/kg BW PO qDay); K2 was positive control group (those given 20% fructose 1,86 g/kg BW PO qDay and standard insulin resistance therapy metformin 500 mg/kg BW PO qDay); the K3 treatment group was given 20% fructose 1,86 g/kg BW PO qDay and celery extract 200 mg/kg BW PO qDay; the K4 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 400 mg/kg BW PO qDay; and the K5 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 600 mg/kg BW PO qDay. The treatment had been given every day for 60 days. Fasting blood glucose levels were measured using a Glucometer. Fasting blood insulin levels were measured using ELISA, HOMA-IR was calculated using a standardized formula, and GLUT4 protein expression was measured using immunohistochemistry. It the end of the intervention, there was a significant decreased in fasting blood glucose (FBG) in K4 group compared with K1 (p <0.05), insulin resistance in K1 was characterized by a higher HOMA-IR value compared to the therapy group, especially K4 and K5 (p <0.05). There was an increase in GLUT-4 expression on K4 and K5 compared with K1 (p<0.05). It can be concluded that celery extract has antihyperglycemia effect and furthermore it can prevent insulin resistance condition.

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Effect of Celery Extract on Fructose Induced Insulin Resistance Rattus norvegicus

Folia Medica Indonesiana ◽

10.20473/fmi.v56i4.24602 ◽

2021 ◽

Vol 56 (4) ◽

pp. 269

Author(s):

Devitya Angielevi Sukarno ◽

Arifa Mustika ◽

Purwo Sri Rejeki

Keyword(s):

Insulin Resistance ◽

Blood Glucose ◽

Treatment Group ◽

Rattus Norvegicus ◽

Therapy Group ◽

Fasting Blood Glucose ◽

Negative Control ◽

Control Group ◽

Glucose Levels ◽

Blood Glucose Levels

Diabetes mellitus (DM) is one of the global health problems and in the top 4th ranks as the main cause of death in developing countries (IDF, 2015). The pathogenesis of type II DM involves abnormalities in insulin secretion and activity that leads to insulin resistance. This research aims to study the efficacy of celery (Apiumgraveolens) as a prevention of insulin resistance. In this study, the samples were 45 Wistar rats (Rattus norvegicus), male sex, aged 4-6 weeks, weight 150-175 grams,and had normal fasting blood glucose levels by tested before treatment. The experimental animals were divided into 5 groups, K1 was negative control group (insulin resistance by given 20% fructose 1,86 g/kg BW PO qDay);K2 was positive control group (those given 20% fructose 1,86 g/kg BW PO qDayand standard insulin resistance therapy metformin 500 mg/kg BW PO qDay); the K3 treatment group was given 20% fructose 1,86 g/kg BW PO qDay and celery extract 200 mg/kg BW PO qDay; the K4 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 400 mg/kg BW PO qDay; and the K5 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 600 mg/kg BW PO qDay. The treatment had been given every day for 60 days.Fasting blood glucose levels were measured using a Glucometer. Fasting blood insulin levels were measured using ELISA, HOMA-IR was calculated using a standardized formula, and GLUT4 protein expression was measured using immunohistochemistry. It the end of the intervention, there was a significant decreased in fasting blood glucose (FBG) in K4 group compared with K1 (p <0.05), insulin resistance in K1 was characterized by a higher HOMA-IR value compared to the therapy group, especially K4 and K5 (p <0.05). There was an increase in GLUT-4 expression on K4 and K5 compared with K1 (p <0.05). It can be concluded that celery extract has antihyperglycemia effect and furthermore it can prevent insulin resistance condition.

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Potency of Swallow Nest Extract (Collocalia fuciphago) as Pancreaprotector Through Oxidative Mechanism in Rats (Rattus norvegicus) Induced by Streptozotocin

Berkala Kedokteran ◽

10.20527/jbk.v14i1.4534 ◽

2018 ◽

Vol 14 (1) ◽

pp. 15

Author(s):

Agnes Frethernety ◽

Satriyandi M ◽

Francisca Diana Alexandra

Keyword(s):

Blood Glucose ◽

Rattus Norvegicus ◽

Fasting Blood Glucose ◽

Water Extract ◽

Clinical Syndrome ◽

Control Group ◽

P Value ◽

Treatment Groups ◽

Glucose Levels ◽

Blood Glucose Levels

Abstract: Diabetes mellitus is a clinical syndrome with characteristics of hyperglycemia. Hyperglycemia can lead to the formation of excessive free radical compounds that trigger oxidative stress, especially on pancreatic β cells. Swallow nest contains amino acids which are insulin-forming compounds and glutathione that can act as a major reducing agent and antioxidant defense. The purpose of this study is to analyze the effect of swallow’s nest water extract as a pancreaprotector through oxidative mechanism in rats (Rattus norvegicus) induced by streptozotocin. The rats induced by streptozotocin at a dose of 40 mg/kg BW and then randomly divided into 4 groups. Control groups were given aquades and 3 treatment groups, respectively were administered swallow’s nest water extract at doses of 1, 10, 100 mg/kg BW for 21 days. Fasting blood glucose levels were measured on 7, 14 and 21 day. MDA measured after the animal is determined at day 28th.Level of fasting blood glucose and MDA were analyzed by statistic using Kruskal Wallis test followed by multiple comparisons Mann Whitney test. There was a significant decrease in fasting blood glucose level with p = 0.04 in all treatment groups compared the control group. The administration of swallow nest extract significantly decreased MDA pancreas with p value = 0.000 and dose 100 mg/kgBW extract of swallow’s nest significantly decrease pancreatic MDA level. The administration of swallow’s nest water extract shows decrease of fasting blood sugar and has a protective effect on the rats’ pancreas through oxidative mechanism. Keywords: Swallow's nest extract, MDA

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Is There A Way to Prevent Aging? A Study Using Metformin in Blood Sugar Levels and Serum Levels of IGF-1

Al-sihah The Public Health Science Journal ◽

10.24252/al-sihah.v13i1.21694 ◽

2021 ◽

Vol 13 (1) ◽

pp. 51

Author(s):

Arlina Wiyata Gama ◽

Arief Santoso ◽

Ika Yustisia ◽

Nurpudji Astuti Taslim ◽

Irfan Idris ◽

...

Keyword(s):

Blood Glucose ◽

Calorie Restriction ◽

Fasting Blood Glucose ◽

White Male ◽

Serum Levels ◽

Male Rats ◽

Control Group ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Reduce Blood Glucose

Aging is influenced by lifestyle, which one is by consuming excessive calories. In recent years there have been many studies on aging and age-related diseases, calorie restriction mimetic is one of them. Metformin is a calorie restriction mimetic that is attractive to gerontologists. This research analyzed the effect of metformin as a calorie restriction mimetic on fasting blood glucose and IGF-1 serum levels in old white male Wistar rats. This study conducted with an experimental study with a pre and post-test pattern with controlled group design on male rats (Rattus novergicus) Wistar strain which was divided into 3 groups, control group (K), the calorie restriction group (P1), and the metformin group (P2), 6 rats each group. Blood glucose levels were measured by a glucometer and serum IGF-1 levels were measured with an ELISA kit, where blood samples were taken from the tail of the rats. In general, the provision of metformin and calorie restriction tended to reduce blood glucose levels, but increased serum IGF-1 levels. There is a significant relationship between blood glucose levels and serum IGF-1 levels. It’s necessary to conduct further research to determine the effective dose and maximum dose of metformin to reduce blood glucose level and serum IG-1 levels which can be slow down the aging process.

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UJI AKTIVITAS ANTIDIABETES EKSTRAK ETANOL BUAH CABAI MERAH ( Capsicum annuum L. ) TERHADAP MENCIT PUTIH JANTAN

SCIENTIA : Jurnal Farmasi dan Kesehatan ◽

10.36434/scientia.v9i1.221 ◽

2019 ◽

Vol 9 (1) ◽

pp. 86 ◽

Cited By ~ 1

Author(s):

Zulkarni Zulkarni

Keyword(s):

Blood Glucose ◽

Capsicum Annuum ◽

Fasting Blood Glucose ◽

Ethanol Extract ◽

Negative Control ◽

Control Group ◽

Glucose Levels ◽

Capsicum Annuum L ◽

Blood Glucose Levels ◽

Significant Difference

This Research was conducted to determine the effect of ethanol extract from red chilli (Capsicum annuum L)in lowering blood glucose levels of hyperglycemic male white mices. This study used 30 malewhitemices and divided into 6 groups: negative control group, the positive control group, the treatmentgroup withdosage of 200 mg / kgweight, 400 mg / kgwieght, 600 mg / kg weight and a comparison group with glibenclamide with dosage of 5 mg / kgweightadministered orally for 21 days. The level of fasting blood glucose was checked 6 days after dexamethasone induced, and after the ethanol extract of red chilies on day 7th, 14th, and 21st. The data was analyzed statistically with one –way and two-way Anova by usingSPSS16 program and proceed with the test Duncan to look the significant difference between treatments. The results showed that the ethanol extract from red chilies with a dosage of 200mg / kgweight, 400mg / kgweight, 600 mg / kgweight showed the effect in lowering blood sugar levels in male white mices hyperglycemia significantly (p <0.05). The duration ethanol extract of red chili affected blood glucose levels. The most effective duration in lowering blood glucose levels is the administration of a preparation within 21 days.

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Study on optimization and stability of a single dose streptozotocin-induced diabetic modeling in rats

10.21203/rs.3.rs-91976/v1 ◽

2020 ◽

Author(s):

Yao Zhang ◽

jiao Zhang ◽

Ming Hong ◽

Jingyi Huang ◽

Rui Wang ◽

...

Keyword(s):

Blood Glucose ◽

High Fat Diet ◽

Female Rats ◽

Male Rats ◽

Control Group ◽

High Fat ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Sd Rats ◽

Male And Female Rats

Abstract BackgroundOptimization of experimental conditions in streptozotocin induced diabetic model in Sprague Dawley (SD) rats to evaluate the stability of the model.MethodsMale and female SD rats were randomly divided into control group, STZ 45 group (STZ: 45 mg / kg), STZ 65 group (STZ: 65 mg / kg), STZ 85 group (STZ: 85 mg / kg), high fat diet with STZ 45 group (STZ: 45 mg / kg), high fat diet with STZ 65 group (STZ: 65 mg / kg), high fat diet with STZ 85 group (STZ: 85 mg / kg). N = 6 in each group. The changes of body weight and blood glucose were observed dynamically.ResultsThere was no significant difference in blood glucose or body weight between the STZ 45 group and the control group in both male and female rats, whether or not they were on a high-fat diet. However, there were significant differences in blood glucose between the high-dose STZ group and the control group in both male and female rats, regardless of whether the rats were on a high-fat diet or not (P < 0.05 or P < 0.01). Compared with the control group, there were significant differences in blood glucose levels (P < 0.05 or P < 0.01) and higher blood glucose levels in the male rats fed with the normal diet than that in those fed with the high-fat diet.ConclusionsIn this study, male rats fed with ordinary feed and injected STZ dose of 65 mg / kg were the most stable and ideal diabetic rat.

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Red dragon fruit (Hylocereus spp.) peel marmalade effectively improve blood glucose and lipid profile of hypercholesterolemic wistar rats

JURNAL GIZI INDONESIA ◽

10.14710/jgi.9.1.61-67 ◽

2020 ◽

Vol 9 (1) ◽

pp. 61-67

Author(s):

Natalia Desy Putriningtyas ◽

Intan Permatasari ◽

Dita Oktaviani ◽

Anastasia Servia Raha ◽

Siti Wahyuningsih

Keyword(s):

Blood Glucose ◽

Functional Food ◽

Wistar Rats ◽

Fasting Blood Glucose ◽

Control Group ◽

Fruit Peel ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Dragon Fruit ◽

Significant Difference

Background: Polyphenols, antioxidants, dietary fiber, and vitamin contained in the red dragon fruit peel. Red dragon fruit peel can be processed into marmalade. Red dragon fruit peel marmalade has the potential to be a functional food. Functional food is food that has a physiological function based on scientific studies.Objectives: The objective of this study is to analyze the effect of red dragon fruit peel marmalade on fasting blood glucose levels, HDL, LDL, and triglycerides levels of hypercholesterolemic Wistar rats.Methods: This study used a pre-&post-test control group design. Hypercholesterolemic male Wistar rats were randomly assigned into five groups. Hypercholesterolemia was induced by 1% cholesterol powder and 0.5% cholic acid for two weeks. All groups received standard chow. Samples were grouped into five groups: K-; K+; K1 (0.94 g/kg b.wt/day); K2 (1.41 g/kg b.wt/day); K3 (1.88 g/kg b.wt/day). The intervention was carried out for 28 days. GDP level was measured using the GOD-PAP. HDL, LDL, and triglyceride were analyzed with spectrophotometry. GDP, HDL, LDL, and triglyceride levels were measured twice before fasting. A paired t-test and one-way ANOVA were used to analyze the data.Results: The result showed that K-; K1; K2; K3 had a significant difference between groups before and after the intervention (p<0.05). Red dragon fruit peel Marmalade was able to reduce the levels of GDP, LDL, triglycerides, and increase HDL (p <0.05).Conclusion: Red dragon fruit peel marmalade reduced fasting blood glucose levels, LDL, triglyceride levels, and increased HDL levels of hypercholesterolemic Wistar rats.

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The Potential of Strawberry, Rome Beauty Apple, and New Combination on Fasting Blood as Supporting Diet Therapy in Patients with Type II Diabetes Mellitus

Global Medical & Health Communication (GMHC) ◽

10.29313/gmhc.v9i1.6409 ◽

2021 ◽

Vol 9 (1) ◽

Author(s):

Niken Yuliwati ◽

Riezky Faisal Nugroho

Keyword(s):

Blood Glucose ◽

Fasting Blood Glucose ◽

Control Group ◽

Type Ii ◽

Control Group Design ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Post Test ◽

Post Hoc ◽

Diabetes Melitus

Strawberries (Fragaria x ananassa) and Rome beauty apples (Malus sylvestris) contain high antioxidants to reduce oxidative stress and improve the glycemic response with diabetes mellitus (DM) type II. This study aimed to analyze the effect of strawberries, Rome beauty apples, and their combination to decrease fasting blood glucose levels of type II DM patients. This experimental research used a randomized pre-post-test with a control group design. Subjects were 44 patients divided into four groups: K (control, not given the fruit), P1 (strawberries 200 g/day), P2 (apples 300 g/day), and P3 (the combination strawberries 100 g/day, apples 150 g/day) for 14 days in February–March 2019 in Surakarta. The inclusion criteria were DM patients aged 40–55 years who received oral antidiabetic drug therapy. Exclusion criteria were type II DM patients who, based on the doctor's diagnosis, had complications such as gastritis, hypertension, chronic renal failure, heart disease, liver disease, stroke, cancer, patients with consumption of antioxidant supplements. Mean of blood glucose level on K (149.82±39.125), P1 (141.00±38.079), P2 (128.73±31.841), and P3 (177.73±50.176). Analysis paired t test results showed a significant difference between before and after treatment (p=0.045). To find out which group was the most meaningful, the Bonferroni post hoc test. Decrease of fasting blood glucose levels with group K (−10.55, p>0.05), P1 (−30.18, p<0.05), P2 (40.27, p<0.05), and P3 (−32.91, p<0.05). ANOVA test was used to analyze, and the post hoc Bonferroni test with a control comparison was performed, resulting in p=0.028, in P2 and p>0.05 in P1 and P3. In conclusion, strawberries, Rome beauty apples, and their combination significantly decreased fasting blood glucose levels of type II DM patients, with the highest and most significant decrease in Rome beauty apples. POTENSI STROBERI, APEL ROME BEAUTY, DAN KOMBINASI BARU TERHADAP GULA DARAH PUASA SEBAGAI PENUNJANG TERAPI DIET PADA PASIEN DIABETES MELITUS TIPE IIStroberi (Fragaria x ananassa) dan apel Rome beauty (Malus sylvestris) mengandung antioksidan tinggi untuk mengurangi stres oksidatif dan memperbaiki respons glikemik pada diabetes melitus (DM) tipe II. Tujuan penelitian adalah menganalisis pengaruh buah stroberi, apel Rome beauty, dan kombinasinya terhadap penurunan kadar glukosa darah puasa pasien DM tipe II. Jenis penelitian eksperimen dengan rancangan randomized pre-post-test dengan control group design. Subjek penelitian 44 pasien yang dibagi empat kelompok, yaitu K (kontrol tidak diberi buah), P1 (stroberi 200 g/hari), P2 (apel Rome beauty 300 g/hari) dan P3 (kombinasi stroberi 100 g/hari dan apel 150 g/hari) selama 14 hari pada bulan Februari–Maret 2020 di Surakarta. Kriteria inklusi adalah pasien DM usia 40–55 tahun yang mendapat terapi obat antidiabetik oral. Kriteria eksklusi adalah pasien DM tipe II yang berdasar atas diagnosis dokter mengalami komplikasi seperti mag, hipertensi, gagal ginjal kronik, penyakit jantung, penyakit hati, strok, kanker, dan pasien dengan konsumsi suplemen antioksidan. Kadar glukosa darah rerata pada K (149,82±39,125), P1 (141,00±38,079), P2 (128,73±31,841), dan P3 (177,73±50,176). Hasil analisis paired t test menunjukkan terdapat perbedaan yang signifikan antara sebelum dan sesudah perlakuan (p=0,045). Untuk mengetahui kelompok mana yang paling bermakna, dilakukan Uji Post Hoc Bonferroni. Penurunan kadar glukosa darah puasa pada K (−10,55; p>0,05), P1 (−30,18; p<0,05), P2 (−40,27; p<0,05), dan P3 (−32,91; p<0,05). Analisis dilakukan dengan uji ANOVA dan Uji Post Hoc Bonferroni dengan perbandingan kontrol menghasilkan p=0,028 pada P2 serta p>0,05 pada P1 dan P3. Simpulan, pemberian stroberi, apel Rome beauty, dan kombinasinya signifikan menurunkan kadar glukosa darah puasa pasien DM tipe II dengan penurunan tertinggi dan bermakna pada apel Rome beauty.

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The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v5i2.12076 ◽

2019 ◽

Vol 5 (2) ◽

pp. 57

Author(s):

Yesi Warisman ◽

Arinda Lironika Suryana ◽

Zora Olivia

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Wistar Rats ◽

Fasting Blood Glucose ◽

Diabetic Rats ◽

Control Group ◽

Standard Diet ◽

Glucose Levels ◽

Blood Glucose Levels

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel. Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

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